جستجوی مقالات مرتبط با کلیدواژه "اثرات ضد باکتریایی" در نشریات گروه "صنایع غذایی"

The use of natural substances could reduce consumption of synthetic agents in food industry. Plants attract the main concentration on this topic, Iran with special conditions has a good potential for growing the plants that have medical application. Zataria multiflora is growing in different part of Iran has antibacterial activity that could be used in food. The aim of this study was to evaluate the antibacterial effect of Zataria multiflora on Aeromonas hydrophila and the use of essence as a coat to improve the shelf life of perca filet. Fresh Zataria multiflora harvested essence was prepared by using the Clevenger apparatus. Then antibacterial property of Zataria multiflora was determined by mirobroth dilution method for measuring the minimal inhibition and bactericidal concentration at laboratory scale. Chemical composition of essence was assayed, also five different levels of essence were used as a food coat in filet. Results showed that MIC and MBC were 250 and 125 µg/ml against Aeromonas hydrophila, respectively. Also results showed that the efficacy of essence extraction was 1.84 percent, and the higher compound in Zataria multiflora essence was thymol (40.5 %). Also, results documented that the use of two level of essence (0.1 and 0.5 percent) of essence in perca filet could statistically increase the shelf life than the control, as well as improve the organoleptic properties. It’s should be concluded that the use of Zataria multiflora essence as food coat by inhibition the bacteria increase shelf life of perca filet at refrigerator storage.

Recently by no incident there is a great concern in people to consume herbal antibiotics rather than using chemical ones due to bacterial resistances witnessed in chemical treatments. In this research the antibacterial activity of aqueous and ethanol extracts of black zira cell suspension culture in comparison with seed extracts and essential oils were investigated against Staphylococus aurous, Escherichia coli and Bacillus subtilis using disk diffusion and digging hole methods. The bacterial concentration was at 106 and 107 CFU/mL. Positive and negative controls were antibiotics (Gentamicin [10 μg/disk], Tetracycline [30 μg/disk]) and solvents (5% DMSO and 80% Ethanol) respectively. Inhibitory effects were studied by measuring the growth inhibiting circle diameter. The Results showed that the range of Minimum Inhibitory Concentration (MIC) were 3.12-6.25, 12.5-50 and 25-50 mg/ml, for the essential oil, the cell extract and the seed extract respectively. According to the results a promising antibacterial activities were revealed from all black zira samples against tested bacteria with the maximum activity to the S. aurous. In contrast to E. coli, the effect of extracts on S. aurous and B. subtilis was dependent to the bacteria concentration. Overall antibacterial effects of cell extracts against tested bacteria were lesser than essential oils (100%) and higher than seed extracts and different dilution of essential oils, therefore it seems cell suspension culture of black zira is a suitable method to produce antibacterial compounds.