جستجوی مقالات مرتبط با کلیدواژه "کدوی پوست کاغذی" در نشریات گروه "منابع طبیعی"

This study was performed to investigate the effect of pumpkin (Cucurbita pepo L.) seed’s pre-treatment by salicylic acid and ascorbic acid on different aspects of seed germination and biochemical features. A factorial experiment was conducted based on a completely randomized design with three replications. Experimental treatments included two levels of seed maturity (normal seeds and aged seeds) and five levels of priming (control, ascorbic acid 50 and 100 mg/l, and salicylic acid 50 and 100 mg/l). The seed traits were finally measured included the germination percentage, first and second seed vigor index, seedling dry weight, peroxidase and superoxide dismutase activity, electrolyte leakage, soluble protein, and soluble carbohydrate. Based on the results, the seed pre-treatment with 100 mg/l ascorbic acid and salicylic acid could perform the highest germination indices. Moreover, the germination percentage (74%) was improved in aged seeds pre-treated by ascorbic acid and salicylic acid (especially at a concentration of 100 mg/l). Also, the antioxidant enzyme activity was increased while the cell electrolyte leakage was significantly decreased. In total, results indicate, the pre-treatment with salicylic acid and ascorbic acid, not only improved the germination quantity and vigor but also improved the germination quality mainly by the development of carbohydrate and protein content in pumpkin seeds.

In order to investigate the effect of sodium nitroprusside (NO donor) on improvement of aged pumpkin seeds under drought stress, a factorial experiment was carried out based on completely randomized design with three replications in Bu-Ali Sina University, Hamedan, Iran. Seed priming with different concentrations of sodium nitroprusside including 0 (hydropriming), 25, 50 and 75 µM in drought stress levels (0, -0.2, -0.46 and -0.6 MPa) was performed. Germination percentage, germination rate, seed vigor, seedling length, soluble carbohydrates, soluble proteins, memberane electrical conductivity, malondialdehyde content and antioxidant enzymes activity (catalase, ascorbate peroxidase and superoxide dismutase) were evaluated. The results showed that seed priming with different concentrations of sodium nitroprusside mitigated significant decrease of germination parameters of aged seeds under drought stress. At -0.6 MPa, seed priming with 75 µM of sodium nitroprusside increased germination rate, vigor index , soluble carbohydrates and proteins 25, 214.1, 90.8, 117.1%, as well as the activity of catalase, superoxide dismutase and ascorbate peroxidase increased by 34, 14.5, 41.4% in comparison to control, respectively. Therefore, seed priming with sodium nitroprusside specialy with 75 µM is suggested for aged pumpkin seeds by decreasing oxidative stress due to drought stress.