جستجوی مقالات مرتبط با کلیدواژه « درماتیت سبوروئیک » در نشریات گروه « پزشکی »

The genus Malassezia yeasts, yeasts are lipophilic Bazydyvmayst as members of the microbial flora of humans and warm-blooded animals often are. Seborrheic dermatitis is a chronic inflammatory disease of the skin surface is observed in adult men. The aim of this study was to investigate the prevalence of Malassezia in Lorestan, Bakhtiari nomads. sampling techniques chip was taken from dandruff head and behind the ears. Sample of 100 subjects (50 healthy subjects and 50 patients with seborrheic dermatitis) and on the Lymyng - Nvtmn agar was modified crops. Macroscopic and microscopic studies were performed on colonies of Malassezia in pure culture. In this study of yeast cells to the total sample of patients with seborrheic dermatitis in 27 (54%) patients, and 17 patients (34%) showed growth of yeast colonies. In healthy individuals, the total amount of yeast cells in the samples, 24 (48%) patients and 14 (28%) showed growth of yeast colonies. The findings showed that Malassezia yeasts in 51% of cases and 31% of the direct examination of the cultures were isolated.

Background and Seborrhoeic dermatitis (SD) is a common skin disorder. Malassezia yeasts have an important role in the etiology of SD. Since anti-fungal agents, especially in azoles are effective for treating SD, in this study, the effect of ketoconazole 2% solution on clinical signs and Malassezia in SD patients were assayed. 100 patients with SD were enrolled in this study. Patients were scored in regard to the severity of lesions at the initial evaluation and every 2 weeks for a 1 month period. Microscopic examination and culture of patients scale in days 0 and 28 were used for isolation and identification of Malassezia species. Patients were divided into two groups (ketoconazole 2% solution and shampoo) and followed after 14 and 28 days, and then clinical response was graded. 58% of patients showed lesions on their heads. In day 0, 51% of patients showed > 7 yeasts in each microscopic field. 77% of scale samples were positive to Malassezia spp. Growth and M. globosa (57.1%) had the most frequency. In day 28, 89.6% and 82.6% of treated patients with solution and shampoo showed 1-3 yeast in within entire smear, respectively. 94.8% and 82.6% of scale samples were negative to Malassezia spp growth, respectively. In day 0, patients with moderate SI had the most prevalence, whereas in day 28, patients with mild SI were predominant. Statistical test showed the correlation is significance only between SI and treatment with solution. The results of our study showed that according to decrease of yeast load and increase of improvement of SD signs after treatment with ketoconazole 2% solution, compared with ketoconazole 2% shampoo, 2% ketoconazole solution can be considered as an appropriate agent in treatment of Sd.

Background and Lipophilic yeast of the genus Malassezia are members of normal human cutaneous micro flora which are also associated with several skin diseases. It is strongly suspected that Malassezia species are responsible for pityriasis versicolor (PV), and seborrhoeic dermatitis (SD). Considering various sensitivities among Malassezia species to antifungal, accurate species identification will facilitate the treatment of relevant diseases. Malassezia species can be identified through their morphological features and bio-chemical characteristics. However, these phenotypic methods are usually time consuming, and lack sufficient discriminatory power. Development of DNA- based methods for detection and identification of Malassezia species provide helpful alternatives for solving problems. The aim of this study was to examine the distribution of Malassezia species in patients with pityriasis versicolor and seborrhoeic dermatitis, using molecular methods. A total of 63 clinical isolates of Malassezia spp, 30 strains isolated from patients with PV and 33 strains isolated from patients with SD, were studied. To investigate the strains at molecular level, genomic DNA of Malassezia isolates were extracted and amplified within the ITS1 region (located between 18S and 5.8S rDNA) by polymerase chain reaction (PCR) assay. DNA sequencing of ITS1 of rDNA in Malassezia spp was performed to type the species. Restriction fragment length polymorphism (RFLP) analysis of ITS1 PCR product with two restrictive enzymes CFOI and BSTF5I, was used in subsequent species identification. In this study, 37 patients with PV (20 females, 17 males 2 to 64 years old), 81.1% (30 case) yielded growth of Malassezia in culture, while the frequency of isolation of M. globosa was 53.3% (16 case), M. furfur 40% (12 case) and M. sympodialis 6.7% (2 case). Of the 41 patients with SD (22 females, 19 males 1 to 52 years old), 80.5% (33 case) yielded growth of Malassezia in culture, while the frequency of M. furfur was 42.4% (14 case), M.globosa 39. 4% (13 case), M. restrict 15.2% (5 case) and M. sympodialis 3% (1 case). This PCR-RFLP Profile allows us to clearly identify important Malassezia species. The results of the PCR-RFLP analyses of clinical isolates were in complete agreement with those from DNA sequencing, morphological features and bio-chemical characterization. In patients with PV, the most frequently isolated species were M. globosa, followed by M. furfur. However, in patients with SD, the most frequently isolated species were M. furfur, followed by M. globosa. The PCR-RFLP system applied for the ITS1 fragment of the rDNA is a reliable, simple and rapid method for identification of the most important Malassezia species, but further work will be necessary in applying these techniques to additional patients.