جستجوی مقالات مرتبط با کلیدواژه "استرپتوزتوسین" در نشریات گروه "پزشکی"

Oxidative stress plays a role in the pathogenesis of Alzheimer's disease. The aim of this study was to investigate the antioxidant effects of sesame oil on oxidative stress parameters caused by administration of streptozotocin in male rats.

This interventional experimental study was performed on five groups (7 on each) of male Wistar rats (250-270 g), including: the control group that did not receive any drug, the sham group that received 5 μl of saline in the lateral ventricles of the brain for 7 days, the streptotocin group which received 5 μl of streptotocin at a dose of 1.5 mg/kg in the lateral ventricles for 7 days, and two groups of streptotocin+sesame oil, which were first pre-treated by intraperitoneal injection of 5 ml/kg of sesame oil in two periods of 7 and 28 days and then received streptotocin. 48 hours after the last drug injection in the experimental groups, the hippocampuses of the rats' brains were separated and homogenized. The activity of antioxidant enzymes glutathione peroxidase, superoxide desmutase, catalase, and the total amount of glutathione in the hippocampus tissue were measured using special kits. Data analysis was done by one-way ANOVA.

The activity of glutathione peroxidase, superoxide dismutase, catalase, and total glutathione in the hippocampal tissue of streptozotocin-treated rats decreased compared to the control group (P <0.05). 28-day pretreatment with sesame oil improved the activity of glutathione peroxidase, superoxide dismutase, catalase and the total amount of hippocampal glutathione in the streptozotocin+sesame oil group to normal values, compared to the streptozotocin group (P<0.05).

By modulating the parameters of oxidative stress in the hippocampus, nutrition of sesame oil may prevent excessive reduction of antioxidant enzymes in the people with or prone to Alzheimer's disease.

Diabetes is one of the major risk factors for ischemic heart disease. The aim of this study was to investigate the effect of antioxidant drug troxerutin (TXR) on ischemia-reperfusion-induced myocardial injury in experimental streptozotocin-induced diabetic rats.

Male Wistar rats (250-270 g) were divided into four groups including control, troxerutin, streptozotocin and streptozotocin + troxerutin groups. Diabetes was induced in the rats by intraperitoneal injection of 50 mg / kg streptozotocin and rats were studied for 10 weeks. Rats in the streptozotocin + tezroxerotin group received troxerotin by oral gavage for 4 weeks from the 7th week onwards. The hearts of the rats in all groups were placed in the Langendrov apparatus after separation from the body and subjected to local ischemia for 30 minutes followed by reperfusion for 60 minutes. The amount of lactate dehydrogenase enzyme in coronary artery as well as the parameters of superoxide dismutase and glutathione peroxidase in myocardial tissue during ischemia-reperfusion were measured using special kits.

Body weight and the amount of superoxide dismutase and glutathione peroxidase enzymes were significantly decreased in the diabetic rats (P <0.05). Troxerutin in the streptozotocin + troxerutin group improved the levels of these enzymes to normal levels (P <0.05), whereas lactate dehydrogenase level decreased in this group (P <0.05).

In this study troxerutin reduced the severity of ischemia-reperfusion injury in the diabetic rats.

Diabetes mellitus is one of the most common serious metabolic disorders characterized by hyperglycemia, altered metabolism of lipids, carbohydrates and proteins. Gestational diabetes mellitus (GDM), affects 3.5–5% of all human pregnancy. Therefore, this study was done to evaluate the effect of gestational diabetes on astrocyte density in CA1 and CA3 subfields of hippocampus in rat male offspring.

In this experimental study, 12 Wistar Dams rats were randomly allocated in control and diabetic groups. Gestational diabetes induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation (GD) in experimental group and controls were received an equivalent volume normal saline injection intraperitoneally (IP). Six male offspring of cases and controls dams, at the 7, 21 postnatal day (P7, P21) were randomly selected. Animals were scarified using chloroform anesthesia. The coronal sections of brain by 6 micrometer serially were prepared. The sections were stained with PTAH. The number of astrocytes was evaluated in 100000 μm2 area of CA1 and CA3 in 1000X magnification. Data was analyzed by SPSS-11.5 and t-test.

In CA1 subfield of hippocampus in offspring, the number of astrocytes significantly reduced by 36.25% and 36.37% in diabetic group in compare to controls in the P7 and P21, respectively (P<0.05). In CA3, astrocytes density significantly reduced 36.35% and 26.5% in GD in comparison with controls in the P7 and P21, respectively (P<0.05).

This study showed that the uncontrol gestational diabetes significantly reduces astrocytes density in CA1 and CA3 subfields of hippocampus in rat offspring.