جستجوی مقالات مرتبط با کلیدواژه "اپی کاتچین" در نشریات گروه "پزشکی"

Today, despite the abundance of chemical drugs, the use of medicinal plants is increasing; Curcumin (curcumin) is a yellow pigment obtained from the turmeric plant and is considered the effective ingredient of turmeric. This crystalline and crystallized product is widely used in Indian medicine. Recently, the beneficial effects of curcumin on stomach, colon, and mouth cancers have been proven in mice. Curcumin usually plays its role through pharmacological processes such as antioxidant, anti-inflammatory, antithrombotic, apoptotic, and hepatoprotective effects. Antioxidative, antiproliferative, and antiangiogenic properties of curcumin have been noticed in recent years. However, the low solubility of curcumin and its severe degradation have made it impossible to introduce it in clinical use. In recent years, studies and reviews have been conducted to prepare and design polymeric micelles (PMMCs) on a nanoscale to improve the cellular transport of curcumin. Green tea flavonoids, including epicatechin, have recently received attention. In in vitro research, these compounds have been proposed as antioxidants by inhibiting lipid peroxidation, trapping free radicals, and chelating metal ions, and this property is mentioned due to their unique structure (therefore, the purpose of this study is to determine the effect The synergism of curcumin and epicatechin has been carried out in the investigation of apoptosis and cell proliferation of HT29 cell lines and apoptotic factors.

This study is experimental. After obtaining HT29 cells from the Pasteur Institute cell bank and completing cell passage and proliferation, the cells were distributed in triplicate wells across 7 test groups and 2 control groups (positive and negative). In test groups 1, 2, and 3, three concentrations of curcumin nanomicelles (10, 20, 50 µg/ml) were incubated in triplicate. In groups 4, 5, and 6, three concentrations of epicatechin (10, 20, 50 µg/ml) were incubated for 24 hours. Test group 7 consisted of HT29 cells treated with the IC50 concentration of nanomicelle curcumin combined with the IC50 concentration of epicatechin. Group 8, the positive control, consisted of cells treated with 5FU (100 µg/ml), while group 9, the negative control, consisted of cells exposed only to the cell culture medium. After the 24-hour incubation period, an MTT assay was performed to assess cell proliferation, and Annexin flow cytometry was used to determine the level of apoptosis.

In this study, epicatechin and curcumin have been able to decrease cell viability of colon cancer cells (HT29) and increase the percent of apoptotic cells P<0.05, which was in a dose-dependent manner, also the combination of these two drugs caused a synergistic effect in high dose P<0.05. Epicatechin in concentrations of 20, 10 μg/mL, 50, and 100 has a significant difference compared to the negative control group with P<0.05, which has caused a decrease in cell viability in colon cancer cells with increasing dose. Curcumin is the group Nanomicelle with epicatechin at IC50 concentration compared to the positive control group of 5-FU in HT-29 cell line has greatly reduced cell viability of HT-29 cell line showed that curcumin and epicatechin nano micelles had a synergistic effect. The results of cell apoptosis have shown that curcumin and epicatechin nano micelle groups at IC50 concentrations had a synergistic effect.

epicatechin and curcumin have been able to increase the death rate of colon cancer cells (HT29) by increasing apoptosis, which indicates the anti-cancer effects of these compounds. So it can suggest these compounds in colon cancer therapeutics.

It is well established that valproic acid (VPA) is teratogenic associated with oxidative stress in humans and in all animal species tested. In this study, considering the chemical composition of catechins, we investigated its protective effect on the survival of PC12 nerve cells exposed to valproic acid.

The protective effects of epicatechin (EC) at different concentrations (10, 50, 100, 500, 1000 μg/ml) on survival and viability of PC12 neuronal cells exposed to valproic acid were measured by MTT assay and oxidative stress tests (GPx, SOD, ROS, and lipid peroxidation). All data were analyzed in GraphPad Prism 8.0 using one-way ANOVA and Tukey post-test.

According to findings, EC reduced the cytotoxic effects of valproic acid at 500 and 1000 µg/ml (P<0.0001). EC significantly reduced the oxidative stress induced by valproic acid via decreasing reactive oxygen species (ROS) and malondialdehyde (MDA) production (P<0.001 and P<0.0001, respectively).

The present study showed that epicatechin could increase antioxidant potential and exhibits significant cytoprotective effect against valproic acid toxicity in PC12 cells.

Catechins in dark chocolate can improve the reduction of muscle mass in the elderly due to the sarcopenia by the positive effect on muscle growth. The purpose of this study was to investigate the effect of eight weeks resistance training and dark chocolate extract supplementation on the plasma levels of Myogenic Factor 5 (Myf5) and muscle strength in the older adults.
In this quasi-experimental study, 36 elderly adults (19 males, 17 females), were objectively selected and randomly divided into four groups: training (RT) (n=8), supplement (S) (n=10), training supplement (RT)(n=9), and control (C)(n=9). Subjects of RT and RT groups underwent a resistance training program. S and RT groups consumed capsules containing 500mg of dark chocolate extract –containing epicatechin- every day. Before and after eight weeks intervention, the plasma levels of Myf5 were measured by ELIZA method and the muscle strength was measured by 1-Repeatation Maximum (1RM) test. The data were analyzed with ANOVA and LSD post hoc tests.
Myf5 values increased significantly in RT, S and RT groups compared to C group after the intervention (P≤0.05). Myf5 values were significantly higher in RT group compared to RT and S groups (P≤0.05). Moreover, chest press and leg press maximal strength increased in RT and RT groups in comparison with C group (P≤0.05).
8-weeks resistance training with the consumption of dark chocolate extract supplement have more efficient effects on the enhancement of plasma myf5 in the older adults compared to mere resistance training.

Exercise training and nutrition are non-pharmacological strategies which can reduce the sarcopenia-induced muscle atrophy in the older adults. Investigating the skeletal muscle mass atrophy is achievable by measuring the amount of plasma follistatin, as one of the key markers of determining lean body mass. Therefore, the aim of this study was to compare the effect of dark chocolate extract supplementation with eight weeks resistance training on plasma Follistatin level in older adults.
in this semi-experimental study, 36 elderly adults (19 males, 17 females), mean age 67.21±4.19 years, randomly divided to four groups: training (EX), supplement (S), training supplement (EX), and control (C). Subjects of the training groups underwent a resistance training program including eight movements (intensity: 60-80% 1RM), three sessions per week for eight weeks. Subjects of supplements groups consumed capsules containing 500mg of dark chocolate extract –contain epicatechin- every day. Follistatin levels were measured before and after eight weeks intervention. The data were analyzed with ANOVA and LSD post hoc tests with SPSS software (version 22).
Follistatin values increased significantly in EX (P=0.01), S (P=0.02) and EX (P=0.001) groups compared to C group after the intervention. The percentage of the changes of follistatin values were significantly higher in EX group (63.15%) compared to EX (43.48) and S (29.05%) groups. Moreover, chest press and leg press maximal strength increased in EX (P=0.03) and EX (P=0.01) groups in comparison with C group (P≤0.05).
Consequently, considering the increasing effect of resistance training and dark chocolate extract supplementation on Follistatin levels in the elderly, it seems that resistance training with dark chocolate extract supplementation is a suitable strategy to reduce the effects of sarcopenia in the older adults.