جستجوی مقالات مرتبط با کلیدواژه "اکینوکوکوس گرانولوزوس" در نشریات گروه "پزشکی"

Colorectal cancer is the third most common cancer in men and the second most common in women. The use of parasites or parasite antigens is one of the newest therapeutic approaches for cancer, although it has not yet been used or approved in human trials. This study aimed to determine the cytotoxic effects of purified carbohydrates from the hyaline layer, fluid, and protoscolices of hydatid cysts on the human colorectal cancer cell line (LS174T).

This descriptive-analytical study was conducted on two sheep and two cow livers infected with hydatid cysts, and the human colorectal adenocarcinoma cell line (LS174T) purchased from the Pasteur Institute of Iran at the Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran during 2022. Cyst fluid, protoscolices, and the hyaline layer were isolated from the hydatid cyst-infected livers, and glycoproteins and glycolipids from different parts of the cyst were extracted using the chloroform-methanol method. Carbohydrates were purified using the beta-elimination method and their presence was confirmed using the phenol-sulfuric acid method. The cytotoxicity of purified carbohydrates on the LS174T cell line was evaluated using the methyl tetrazolium (MTT) assay for 24 hours.

Concentrations of 0.5 and 0.25 of purified carbohydrates from glycoproteins and glycolipids of hydatid cysts significantly inhibited the growth of LS174T cells (P<0.05). Initial cytotoxicity evaluation showed that purified carbohydrates from glycoproteins of sheep cyst fluid (38.60±1.33%), glycoproteins of cow cyst hyaline layer (36.61±1.76%), glycolipids of cow cyst hyaline layer (38.94±2.99%), glycoproteins of cow cyst protoscolices (40.27±3.66%), and glycolipids of sheep cyst protoscolices (37.94±1.99%) had significantly more pronounced inhibitory effects (P<0.05). Subsequent screening with the MTT assay revealed that purified carbohydrates from glycoproteins isolated from sheep hydatid cyst fluid (52.35±10.24%), glycolipids isolated from cow hydatid cyst hyaline layer (53.40±7.88%), and glycolipids isolated from sheep hydatid cyst protoscolices (53.05±5.06%) had significantly more pronounced inhibitory effects on the growth of LS174T cancer cells (P<0.05).

Carbohydrates isolated from glycoproteins and glycolipids of the hyaline layer, fluid, and protoscolices of hydatid cysts have significant anticancer effects on the LS174T colorectal cancer cell line.

Hydatidosis is one of the most common zoonotic parasitic diseases in the world, which is also endemic in Iran. Because no seroprevalence study of Hydatidosis has been conducted in Borujerd, this study was conducted with the aim of investigating the seroprevalence of hydatidosis and its associated risk factors among individuals attending comprehensive health centers in Borujerd, Iran, using the enzyme-linked immunosorbent assay (ELISA) method.

This descriptive-cross-sectional study was conducted on 351 serum samples from individuals attending comprehensive health centers in Borujerd city. After obtaining informed consent and completing a questionnaire, blood samples were collected, and the presence of anti-Echinococcus granulosus IgG antibody was detected by ELISA method. Data were analyzed using chi-square and Fisher's exact tests.

The findings indicated that 14 out of 351 subjects tested were positive for anti-Echinococcus antibodies, with seropositivity rates of 5.1% in men and 2.8% in women. No significant associations were observed between the presence of anti-Echinococcus antibodies and variables such as age, gender, education level, occupation, place of residence, contact with dogs, and vegetable washing practices.

The results highlighted a notable seroprevalence of hydatidosis in Borujerd (4%), which is higher than the average of infection rate in the west of Iran. Considering the endemicity of the disease in this region and also its health importance, taking necessary measures, including hygienic slaughter of livestock, prevention of feeding dogs with contaminated intestines and viscera, and collecting stray dogs in order to control and prevent this parasitic infection.

Hydatidosis is one of the most important zoonotic infections that is a health-economic concern in endemic areas. This study aimed to determine the seroprevalence of hydatidosis in Arak, Iran.

This descriptive cross-sectional study was conducted on 456 individuals attending health centers in Arak in 2020. Blood samples were taken from the subjects and tested for anti-Echinococcus antibodies by the enzyme-linked immunosorbent assay-immunoglobulin G test. The collected data were then analyzed.

It was revealed that 6 out of 456 subjects had anti-Echinococcus antibodies (1.3%, 95% confidence interval: 0.3-2.3) The mean age of the participants was 58.8±19.9 years, and frequencies of seropositive individuals were 1.6% and 1% in men and women, respectively. Moreover, 5 (1.4%) and 1 (1%) urban and rural residents were found seropositive, respectively. According to the results, 93.4% of the participants were unaware of hydatid cyst disease and 21.5% of the cases had contact with dogs (P<0.05). It was found that 213 (46.7%) individuals used only water or saltwater to wash vegetables, and all seropositive subjects were observed in this group (P=0.01).

The results of this study showed that the seroprevalence of hydatidosis was significant in Arak. Therefore, due to the health importance of hydatidosis and its endemicity in this area, it is necessary to adopt health measures to control and prevent this parasitic infection.

The common etiology of hydatid cysts in the world is Echinococcus granulosus. The first organ that is usually involved is liver, and the common GI manifestations are abdominal pain, hepatomegaly, anorexia, vomiting and jaundice. Pelvic cavity is rarely involved initially and the symptoms that patients often present are because of the pressure to adjacent organs such as bladder or rectum and bowel.

A 22 years old woman with anorexia and hypogastric pain was admitted in Imam Reza hospital in Mashhad. In abdominopelvic sonography, a cystic lesion with fine septa and low level echoes was seen in pelvic cavity and in CT scan a cystic lesion with fat density was seen in pelvic cavity. The patient was taken to operating room with possibly diagnosis of dermoid cyst of left ovary, but during operation, hydatid cyst was seen in pelvic cavity and was confirmed by pathology.

In endemic countries for hydatid disease, the hydatid cyst should be considered in differential diagnosis of any cystic lesion in any organ.

Hydatidosis is one of the most prevalent zoonotic diseases around the world that is endemic in Iran too. In rural areas, there is a higher risk of infection. To the best of our knowledge, no study was done on seroprevalence of hydatidosis in Qaemshahr, north of Iran, so, we aimed to investigate this condition and its risk factors in patients attending Qaemshahr rural health centers using ELISA method.

A total of 403 serum samples were collected. The participants completed a researcher-made questionnaire. Then, anti-echinococcus granulosus antibody was analyzed using ELISA method. Data analysis was done applying logistic regression in SPSS V24.

Eleven cases (2.73%) were found positive for anti-echinococcus granulosus antibody. There were no significant differences between seropositivity and gender, consumption of raw vegetable, non-chlorinated water and contact with dog and soil (P>0.05), but significant relationships were seen between anti-echinococcus granulosus antibody and abdominal pain and consumption of wild vegetables (P< 0.05).

In this study, the prevalence of hydatidosis was similar to other parts of Iran. Further studies considering lifestyle and other variables are suggested in all areas of Mazandaran province with similar climate to Qaemshahr.

Cancer is a disease that starts from abnormal proliferation of cells in the body and occurs in different tissues of the host body. Cancer treatment is currently being treated with chemical drugs and radiation that can have long-term complications for the patient. The aim of this study was to investigate the anticancer effects of hydatid cyst antigens on SK-BR-3 cancer cell lines.

In this study, hydatid cysts were collected from slaughterhouses in the province of Isfahan and the fluid removed with Protoscolex. Crude antigens, cyst fluid antigens and excretory-secretory antigens were extracted. SK-BR-3 cell line was cultured in RPMI medium. Different antigen fractions were added to the cells in 96-well plates and after 48 hours of treatment, cell viability was assessed using MTT assay.

All fractions had acceptable anti-cancer effects compared to the control group, which was statistically significant. The fraction of 60% of ammonium sulfate of the crude antigen had better anticancer effects than the 20% and 40% fraction, but in the liquid cyst antigen and the excretory-secretory antigen, the anticancer activity of the ammonium sulfate 40% fraction was higher than the other fractions.

The results of the present study showed that antigens isolated from human hydatid cysts have anticancer effects on SK-BR-3 cancer cell line and can be used in future studies as candidates for cancer prevention.

Infection with hydatid cysts or hydatidosis, which is a zoonotic disease, is caused by the larval stage of Echinococcus granulosus in humans and animals. Hydatidosis infection is endemic in most parts of the country. In Khoda Afarin County in East Azarbaijan, Iran, as a center of animal husbandry, there is a possibility of high exposure to the Echinococcus granulosus egg. This study was carried out to determine the seroprevalence of hydatidosis in this county population.

In this descriptive cross-sectional study, 295 blood samples were collected from the population of Khoda Afarin County in a test tube without anticoagulant within 2018 to 2019. The samples were evaluated using enzyme-linked immunosorbent assay to measure immunoglobulin G against Echinococcus granulosus. The demographic data of the participants were gathered by a questionnaire, and the results were analyzed using SPSS software (version 18).

Out of 295 samples, 12 (4.07%) people were positive. The highest percentage of seropositivity was reported in the age group of 30-39 years with 3 (3.53%) cases. The frequency rates of seropositivity in urban and rural subjects were 16.7% and 83.3%, respectively.

The obtained results showed a significant prevalence of infection in the study population (similar to those reported in some other studies carried out in different parts of Iran). Therefore, it is essential to focus on the control and prevention of the disease as a priority disease in Khoda Afarin County among different occupations and age groups.

Echinococcosis garnulosus is a major zoonotic disease, and surgery is the best treatment for it. So far, several chemical scolicidals have been used to prevent protoscolices leakage during surgery, but due to their adverse effects were not well-received. Therefore, using medicinal plants or compounds driven from them as alternatives were taken into consideration. This study was conducted to compare the in vitro effect of Cannabis sativa essential oil with albendazole on protoscolices of hydatid cyst.

In this descriptive-analytic study, essential oil of C. sativa was prepared from aerial parts of the plant in flowering season in university of Camerino, Italy, and Gas chromatography – mass spectrometry (GC-MS) analysis was performed. Protoscolices were extracted from the livers infected with hydatid cyst and were exposed to different concentrations of C. sativa (1, 2, 5 and 10 μg/ml) for 10, 30, 60 and 120 minutes. The viability of protoscolices was measured by 0.1% eosin staining. Albendazole was used as a standard medicinal drug.

Essential oil of C. sativa at all tested concentrations led to significant mortality in protoscolices which showed a direct relation with increase in concentration (P<0.05). The highest concentration of albendazole after 2 h led to 13.24% mortality rate while, this amount with C. sativa essential oil at 2µg/ml reached to 20.9%, and at 10µg/ml reached to 26.08%.

This study showed that C. sativa essential oil has promising scolicidal effect compared to albendazole and can be suggested as a natural protoscolicidal agent.

This study aims to evaluate the scolicidal effects of oak (Quercus infectoria) extract on hydatid cyst protoscoleces in vitro and ex vivo.

Following the collection of protoscoleces from sheep livers infected with fertile hydatid cysts from a slaughterhouse in Khorramabad, they were treated with various concentrations of oak extract (250-1000 µg/mL) for 5-40 min in vitro and ex vivo. Finally the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining).

The mean of the mortality of protoscoleces was 100% after 5 min incubation with the concentration of 1000 mg/ml of oak extract. On the other hand, the mean of the mortality of protoscoleces after 10 incubation with the concentration of 500 mg/ml of oak extract was 100%. However, these results were not observed in the ex vivo analysis. That is, oak extract requires a longer length of time to display potent protoscolicidal effects.

The results of this investigation revealed that oak extract could have a significant scolicidal activity on hydatid cyst protoscoleces. However, further research, particularly in human and animal subjects, is required to reach this conclusion.
Keywords: Hydatidosis, Echinococcus granulosus, extract, hydatid sand, hcolicidal.

Cystic echinococcosis (CE) is a zoonotic infection caused Echinococcus granulosus with worldwide distribution. As one of the problems that can be encountered after treating CE patients is the risk of postsurgical relapses or treatment failure, a long-term clinical and serological follow-up is required to evaluate the success and failure of therapy. We used extract protoscolex antigen from fresh sheep hydatid cyst collect from slaughtered in Ahvaz. Overall we tested 180 serum samples comprising: 41 sera from post surgically confirmed CE from 6 month to 20 years after surgery, 69 sera from patients with symptomatic CE and 50 sera from non CE patients and 20 heterologous sera were evaluated with SDS-PAGE gel electrophoresis and western blotting. The sensitive of the 38 KDa PSC Antigen was 67 % pre and 83 % after surgery and 100% specify. All hepatic CE and 66.6 % of pulmonary CE sera was reacted with PSC Antigen. The results indicate that the hydatid specific antibodies of IgG, IgG1 and IgG4 are the most important antibodies for the serological diagnosis of CE during the active stage of the disease and Ig G, particularly sub-class Ig G4 was more suitable for postoperative follow up.

  Hydatid disease of urinary tract is limited to renal involvement by echinococcus granolosus. Involvement of other parts of the urinary tract has been limited. However, the other types of echinococcuses are rare. The aim of this study was to report experiences with renal hydatid cyst, and a brief review of articles.   In the present study, 16 patients aged 9 to 61 were operated in Yasuj, Iran, from September 2001 to September 2005. Diagnostic methods included ultrasound, CT scan, MRI and serologic tests. Fourteen patients were diagnosed by open surgery with peristaltic neoplasia through peristaltic peristalsis and 2 patients diagnosed with renal tumor, but their pathology was diagnosed. In addition, in this study review was carried out of the published English-language studies in Pabmind and Scopus.   The mean age of the patients 39 years (9 to 61 years), the ratio of women to men, serologic tests were negative in both cases, which were negative for nephrectomy, ultrasound, CT scan and MRI had no specific differentiation for renal hydatid cyst.   Renal hydatid cyst is still one of the health problems that should be considered. Due to advances in imaging techniques, rapid diagnosis of kidney hydatid cyst is possible, but renal hydatid cyst should be rejected in any cystic mass in the kidney, especially in endemic areas before surgery.

Hydatidosis is one the most important zoonotic infections that is a critical issue of health economics in endemic areas. The aim of this study was to estimate the prevalence level of hydatidosis and parasitic disease encounters in Hamadan. This cross sectional study was conducted on 1000 individuals referring to health centers in Hamadan in 2017. The samples and demographic data had been collected before the serum samples were subjected to anti-Echinococcus IgG antibody detection by ELISA method. Data were analyzed using SPSS and Fisher's exact test. Out of the total participants, four subjects (two males and two females, 0.4%) were considered positive for anti-Echinococcus antibody. Two of the seropositive individuals were residing in rural and the other two subjects were living in urban areas. In addition, about ninety nine percent of the cases had no knowledge of hydatid cyst disease. Three of the seropositive subjects had a history of contact with dog (29.3%). Moreover, they used water for washing raw vegetables (36.2%). The prevalence level of hydatidosis in this area is lower than that of other regions in Iran. However, it is a must to implement management and prevention programs to control and reduce the infection levels in the country due to the endemicity and health economics importance of this issue.

Hydatidosis is one of the most important zoonotic diseases with global distribution. Due to the prevalence of the disease in country, the study aimed to determine the clinical and laboratory findings in patients with hydatidosis admitted to surgical departments of educational hospitals, Imam Khomeini and Golestan, in Ahvaz city during 2004-2014.
Data of the patients including demographic characteristics, and clinical and laboratory findings were recorded in a prepared form and analyzed using descriptive statistics and Chi-square tests by the SPSS software.
From a total of all patients, 49 (55.7%) and 39 patients (44.3%) were females and males respectively; 25 patients were resident in rural areas and 62 in urban areas. The mean age of the patients was 30.6±2 years. Also, the maximum and minimum percentages of the disease were observed in the age group 20-29 and 80-89, respectively. Recurrence was observed in 9 patients. The highest prevalence of the hydatid cyst was in the right lobe of the liver (26.1%). Among the methods of imaging, sonography and MRI had the highest and lowest use in the diagnosis of the disease, respectively.
Findings of this study show that the most common organs affected are the liver and the younger age group and suburbs are more prone to hydatidosis. Due to the chronicity of the disease and the late diagnosis during the visit, screening for endemic areas and high-risk persons is recommended.

Hydatidosis is one of the most important zoonotic parasitic disease worldwide caused by Echinococcus granulosus. Given the importance of parasite genotyping in disease control, this study was aimed to evaluate the genetic variation of the camel and dog isolates in Isfahan.
Material and This cross-sectional study was done on infected livers and lungs of camels with hydatid cyst (n=51) during 2015-2016, collected from Zarinshahr and Najafabad industrial abattoir and 200 adult E. granulosus worms collected from stray dogs in different geographical regions of Isfahan for the molecular identification of mitochondrial genes (CO1, ND1). The DNA of protoscolices and adult worms were extracted, the PCR and sequencing were done using specific primers. Pairwise sequence alignment was used to identify the genetic similarity between the sequences and the registered ones in Gen bank using Clustalw2 software.
The PCR and sequencing of CO1 and ND1 genes on dog and camel samples showed different E. granulosus genotypes, including the common sheep strain (G1), bovid strain(G3) and camel strain (G6) in Isfahan region. The most frequent strains were the common sheep strain (G1). The genetic similarity between both ND1 and CO1 isolates taken from camel, dog and the isolates registered in Gene bank were 30.25-100% and 27.47-100% for camel and dog, respectively.
Mitochondrial ND1 and CO1 genes are proper molecular markers for identifying the genetic variation of the camel and dog E. granulosus. The dominant strain of the worm in Isfahan, is the same as the other parts of the country, i.e., the G1 genotype (the most common sheep strain) belonged to the G1- G1 E. granulosus complex.

Hydatidosisis one of the most common diseases of human and animals from Cestoda class with worldwide distribution. This disease of both public health and economic damage is very important. This parasite has a high genetic diversity and includes a complex of different genotypes .Usually in areas where disease is endemic; biologically there is a relatively high genetic diversity in parasites. Studies in different parts of the world demonstrate that genotype variation and the nature of Echinococcus granulosus influenced on the life cycle of parasite, transmission routes, pathogenesis, and antigenicity, immunogenicity, response to medication, epidemiology and control of the disease. The main aim of the present study was to investigate genotype diversity of E. granulosus which was isolated from stray dogs in Ilam city, West of Iran.
Adult worms were collected from the small intestine of the stray dogs. DNA was extracted from the adult worms and the partial mitochondrial DNA Cytochrome Oxidasesubunit 1 (Cox1) was amplified by PCR then the products were digested by using Alu1 and HpaII restriction enzymes. Finally, a number of PCR products were bidirectionally sequenced. Sequence and phylogenetic analyses were performed using MEGA6 (Mega software. net).
Findings: Totally, twenty stray dogs out of 75 (26.66%) showed infection with E. granulosus. Amplified PCR product for all isolates was a band of approximately 450bp. Alu1 digested the product into two bands of approximately 175bp and 275bp fragments, while the HpaII cut the product into 265bp and 185bp fragments for all dog samples. Based on the DNA sequencing and PCR-RFLP results, dog samples indicated to pertain the genotypic similarities. According to the phylogenetic tree, there is at least one genotype of parasite, which belongs to E. granulosous sensu stricto (G1–G3) complex and overall isolates sequences of mtDNA indicated 100 % homology with references G1, G2, and G3 sequences in the GenBank database.
Discussion & The results of this study indicated that genotypic similarities between the sizes of DNA bands of E. granulosus from all isolates with PCR-RFLP and sequencing methods indicated the occurrence of similarities genotypes of E.granulosus in Ilam region. There is at least one genotype of parasite, which belongs to E. granulosous sensu stricto(G1-G3)complex.

Treatment of hydatidosis in humans, mainly surgery with the use of drugs is combined. During surgery, the risk of fluid leakage hydatid cyst that was content to adjacent tissues, resulting in recurrence of the disease. Ephedra is a plant that grows in various regions of Iran and used to treat as antibacterial and antifungal in certain diseases. The aim of this study was to in vitro evaluate the effects of different concentrations of aqueous extract of Ephedra major at different times.
Infected liver to hydatid cysts were collected from slaughterhouses. Protoscoleces viability was assessed by staining Eosin 1.0 percent. Protoscoleces activities of aqueous extract 1/0, 01/0, 001/0 mg/ml at 10, 20, 30 and 60 minutes were analyzed. Saline and saturated brine were used as positive and negative controls.
The highest percentage of household protoscoleces dilution of 0.1 (13.58%) was observed (P= 0.001). Protoscoleces lowest scolicidal effect rate was found in the dilution 0.001 (2.47%)
The effect of aqueous extract of Ephedra major plumbing protoscoleces not significant. Therefore, it cannot be used as a cache protoscoleces strong and appropriate.

Background & Hydatidosis is a zoonotic disease that caused by infection with the larvae of Echinococcus granulosus. Different antigens produced in larval stage of this parasite that recombinant vaccine base these antigens created significant immunity in infected animals. One of the important antigens is p14-3-3 that it's recombinant antigen created considerable immunity in mouse models. In this study according to the high immunity of antigen epitopes region the coding sequence of T-cell epitopes of P14-3-3 was cloned into pEGFP-N1vector in order to produce an effective DNA vaccine model to stimulate high level of Th1 immune response. In this study bioinformatics tools were used to prediction of linear T-Cell epitopes of Echinococcus granulosus P14-3-3 ζ antigen. The nucleotide coding sequence of these epitopes was synthesized by PCR. the ampliqon was digested with XhoI restriction enzyme and cloned into pEGFP–N1 vector That has been purificated by modified sambrook method with CaCl2 and PEG6000..Positive colony was selected by direct colony PCR and confirmed by the sequencing.and evaluation of it's expression in Eukaryotic cells was done by transformed to CHO cell line with electroporation. Linear T-cell epitopes of Echinococcus granulosus P14-3-3 after prediction,synthesis and amplification wae successfully cloned into pEGFP-N1 vector that purificated by new method with maximum vector and minimum RNA concentration.The expression of new constract in CHO cell line as a eukaryotic cells achivment by fluorescent microscope and will be used as a DNA vaccine model to evaluation immuno response in mouse models. Successfully cloning of The linear T-cell epitppes coding sequence of Echinococcus granulosus P14-3-3ζ antigen into pEGFP-N1 verificated by sequencing and fluorscent microscope images demonstrated expression of recombinant protein in CHO cell line.

Echinococcosis disease is one of the most common diseases of human and animals in many parts of the world, including Iran, which is caused by larval stage of Echinococcus granulosus. This parasite has a high genetic diversity and including a complex of different genotypes (strains). Usually in areas where disease is endemic, there is a relatively high genetic diversity in parasite biologically. Studies in different parts of the world demonstrate that genotype variation and the nature of E.granulosus influenced on the life cycle of parasite, transmission routes, pathogenesis, and antigenicity, immunogenicity, response to medication, epidemiology and control of the disease. The aim of present study was to determination of genotype isolates of human and sheep hydatid in Ilam province for the first time.

20 hydatid cyst samples were collected from sheep Ilam Industrial slaughterhouses and 10 human samples from Ilam hospitals. After DNA extraction of protoscoleces, mitDNA NADH dehydrogenase subunit 1 (nad-1) areas was amplified by PCR and the PCR products were analyzed by PCR-RFLP.

Based on the results which obtained, the size of proliferative nad-1 products was 550 base pairs. Patterns of parts which obtained from PCR products after cutting by AluI and RsaI enzymes showed that all samples had a similar RFLP pattern but HpaII enzyme did not cut any region and had no change in fragment size.

The results of this study indicated that genotypic similarities between the size of DNA bands of E. granulosus from human and sheep isolates with PCR-RFLP method indicated the occurrence of similarities genotypes of E.granulosus in Ilam region. There is at least one genotype of parasite, which belongs to E. granulosous sensu stricto (G1-G3) complex.

Background and Hydatidosis is a parasitic disease that transmits by Echinococcusgranulosus eggs excreted from the feces of infected dogs to humans and animals. This parasite is one of the tapeworms of the family Taeniidae which causes widespread health problems among humans and also economic loss in animal. Therefore, designing a new generation vaccine is important to prevent the disease. In this study, the linear T-cell epitopes were selected. The coding sequence of epitopes was synthesized and cloned to pEGFP-N1vector. The recombinant vector was transfected into CHO cells by genpulser. Expression of fusion Protein was confirmed by fluorescence microscopy. The accuracy of cloning was confirmed by both colony PCR on the clone and the subsequent sequencing. Fluorescence microscopy showed fusion protein was expressed in CHO. The results showed that, linear T-cell epitope of EG95 antigen was cloned correctly in pEGFP vector and its expression confirmed that this recombinant vector can be used to construct a DNA vaccine model against the Echinococcusgranulosus.