جستجوی مقالات مرتبط با کلیدواژه "بیفیدوباکتریوم" در نشریات گروه "پزشکی"

Probiotics are living microorganisms that, if prescribed in sufficient quantity, have beneficial effects on the host's health. Consuming a large number of live bacteria increases the possibility of infection and inflammatory reactions, especially in people with a weak immune system. Therefore, in recent years, new terms such as postbiotics have been added to the literature of researchers. This study aimed to investigate the antimicrobial effect of the culture supernatant of three probiotic strains of Bifidobacterium as a postbiotic on the growth of Pseudomonas aeruginosa bacteria in laboratory conditions.

In order to conduct this experimental study, three probiotic strains of Bifidobacterium bifidum, Bifidobacterium longum, and Bifidobacterium lactis as well as Pseudomonas aeruginosa, were cultivated. Based on the standards, the lack of contamination of probiotic culture was checked and finally, after preparing the culture supernatant of probiotics, the effect of each probiotic strain on inhibiting the growth of Pseudomonas bacteria was compared to the negative and positive control.

  The greatest inhibition of growth was achieved by Bifidobacterium bifidum. (16/33±1.52mm). No significant difference was observed between groups Bifidobacterium bifidum and positive control. The synergistic effect between probiotics was not seen.

Probiotics and postbiotics can act as natural antibiotics against harmful pathogens. For appropriate and targeted exploitation, it seems necessary to identify a strain with a better effect on each pathogen.

Production of functional foods is one of the most important achievements in food industry and using these products provides the convergence of two important events in life; namely receiving nutrients from the diet and promoting health for consumers at the same time. This study aimed to verify the viability of microorganisms and stability of these microorganisms in digestive conditions of the body in order to benefit from the claimed properties of these products.

In this experimental study, 14 samples of probiotic yogurts were collected from three dairy factories in Mazandaran province in summer 2020. The test method consisted of three main steps; determining the viability of probiotic bacteria in the samples over time, then simulating the conditions of the gastrointestinal conditions, including pH changes and exposure to pepsin and bile salts, and finally, evaluating the resistance of microorganisms after passing the conditions of gastric and intestinal simulated condition. Statistical analysis was performed using SPSS.

In the study of viability of Bifidobacterium and Lactobacillus, a significant difference was observed between the mean number of colonies both at different weeks and different pH levels (P<0.05). Yet, the number of probiotic bacteria remained within acceptable standard range (106 cfu/g) over time and did not show a considerable reduction even after passing the acidic and alkaline conditions and tolerating the simulated digestive conditions.

According to this study, if strains (probiotic bacteria) resistant to acid and bile are selected and also suitable environmental conditions are used, consumption of probiotic yogurts can be of great benefit in improving the health of consumers.

Intestinal colonization of the newborn is essential for establishment, maturation and maintenance of the gut mucosal barrier. The greatest difference between the microbiota of breast milk and Infant formula infants feeding is the numbers and species composition of Bifidobacteria. In this study, we tried to identify the native Bifidobacterium isolates obtained from the human’s breast milk and the feces of their paired infants in rural areas of Markazi Province in 2015. In this descriptive study, 28 samples from mothers’ milk and 28 samples from paired infants feces were collected and cultured. Suspicious colonies were picked up and confirmed by phenotypic identification and finally specific primers were designed for genotypic detection by PCR assay. Finally, the results were analyzed using spss18 software. Findings: In this study, amongst Fifty-six samples, 31 (55%) different Bifidobacterium species including 15 (36%) B. bifidum, 14 (34%) B. longum, and, 12 (29%) B. 1 were isolated. Out of which, 12 (29%) isolates including B. longum (6), B. breve (4) and B. bifidum (2) were shared between six mother-infant pairs. The correlation coefficient of bifidobacteria isolated from breast milk against their feces was +0.821 (p-value <0.05). By observing the 29 percent contribution and the positive correlation coefficient between breast milk and fecal bifidobacterium in our study, it probably be acknowledged that the main source of intestinal bifidobacterium in the early stages of birth is breast milk.

Probiotic milk products with valuable nutritional and health properties is one the most important issues of nutrition and medicine. The aim of present study was to produce chocolate milk containing microencapsulated probiotic bacteria.
In the present study, the Lactobacillus casei and Bifidobacterium animalis were injected into chocolate milk both in free and microencapsulated forms. Sodium alginate and resistant starch were used for microencapsulation via extrusion method. The changes in probiotic bacteria count and their sensory acceptability were evaluated at 5°C for 21 days. Also, survival rate of bacteria was determined in simulated gastrointestinal conditions.
The significant survival rates of microencapsulated bacteria was observed in chocolate milk and also in simulated gastrointestinal condition in all conditions (p Based on the sensory physicochemical evaluations, the chocolate milk can be produced containing microencapsulated Lactobacillus casei and Bifidobacterium animalis with expiration time of 6 and 9 days, respectively. Given the important role of such products in disease prevention and boosting the immune system, so the use of them is recommended in time of war.

Obesity and Diabetes are two metabolic diseases that are dependent on genetic and various environmental factors. Today، obesity and type 2 diabetes cannot just be attributed to genome changes، bad eating habits، or physical activity reduction. Gut microflora is another effective factor that has an important role in health. Recently، numerous experiments have been done to investigate the relation between metabolic diseases and bacterial populations in the gut. The purpose of this study is to assess the bifidobacteria of the intestinal microbiota in people with type 2 diabetes and compare with healthy persons. In this study، 40 male and female adults، 20 of whom were diagnosed with type 2 diabetes، were studied. For this study، age، gender، and body-mass index (BMI) are matched in diabetic and healthy persons. After fecal sample collection from diabetic and healthy persons، and extraction of DNA from samples، culture and extraction of DNA from standard bifidobacteria were done as a positive control. After that، cloning in PTZ57 R/T plasmid and extraction cloning plasmid was done. For quantitative investigation of bifidobacteria، Real-time PCR molecular technique was done by using special primer of bifidobacterium and standard curve. To study the number of bifidobacteria in people with type 2 diabetes and compare with healthy persons، statistical analysis was done. Average number of bacteria in people with type 2 diabetes is more than in healthy persons، but Student’s independent t-test) P = 0. 09) and Mann-Whitney test (P = 0. 1) indicate that there are no meaningful differences between them. Quantitative calculation with Real-time PCR indicates that the number of bifidobacteria is not effective in Iranian type 2 diabetes patients.

Probiotic bacteria is added directly to food components and it has beneficial effect on function and the health of organisms. The bifidogenic factors enter the colon where they contribute to an increase lactic acid bacteria population including Lactobacilli and Bifidobacteria and they inhibit enteric pathogenic bacterial growth. The aim of this study is to investigate the effect of culture medium on metabolic and antibacterial of probiotic bacteria.

In this study، the probiotics bacterial and intestine pathogenic are to be used. Lactobacilli and Bifidobacterium were identified by plating samples on MRS medium، Gram Staining and standard biochemical methods. The effect of antagonistic probiotics was investigated in the presence of growth factor in the method well diffusion Ager on the Shigella flexneri (PTCC 1234)، Escherichia coli (PTCC 1552)، Salmonella typhi (PTCC 1609) and the culture medium pH was measured.

The probiotics bacterial growth in MRS and lactose1%، sorbitol، raffinose، riboflavin were shown the effect antibacterial. The results of the study show the most antagonistic activity in commercial strain Lactobacillus acidophilus on Shigella flexneri and lower activity was in Lactobacillus casei (PTCC 1608)، and Salmonella typhimurium (PTCC 1609)، and also in Bbifidobacterium bifidum، it showed the most decrease pH value.

According to the result of the study، adding growth factors to MRS medium base and lactose 1%، probiotic growth was increased and which also increased antagonistic activity.

Probiotics are viable microorganisms which improve human health by inhibiting or reducing the number of pathogenic microorganisms. The most common probiotic strains are Lactobacilli and Bifidobacterium. Varying results have been reported about the effect of different probiotic bacterial strains on oral health and decrease in the counts of salivary Streptococcus mutans strains in previous studies. To have a favorable effect on oral health, a probiotic must have the capacity to adhere to tooth surfaces and must be integrated in the bacterial biofilm; it should also be able to compete with cariogenic bacteria and prevent their proliferation. Permanent and daily consumption of probiotics has been recommended for optimal results. Studies have suggested that probiotics can improve the oral cavity microbial equilibrium and the probable benefits increase with an early childhood use; however, caution should be exercised in the administration of probiotics in children because their oral microflora is not been established yet. Although bacteriotherapy with probiotics seems to have inhibitory effects on pathogenic oral microorganisms, further studies are necessary before any recommendations on their clinical use. The aim of this study was to review probiotics, mechanisms of their function, therapeutic effects, application techniques, their cariostatic effects and a review of randomized controlled clinical trials in this regard.

For the first time in Iran 40 strains of Bifidobacterium were isolated from faces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and 1 as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, 2 methods of PCR with genus-specific primers of Bif164f and Bif601r and 16SrRNA gene sequence analysis were applied. Results of PCR confirmed the obtained phenotypic identifications. Moreover by this method the 8 remaining strains were identified as Bifidobacterium species. Using sequencing 16SrRNA gene, 5 B.Longum strains were identified that had different fermentation pattern from B.Longum. Some new ribose negative Bifidobacterium longum strains were also identified. The obtained results present new strains of Bifidobacterium longum.