جستجوی مقالات مرتبط با کلیدواژه « تحرک اسپرم » در نشریات گروه « پزشکی »

Asthenozoospermia is an infertility condition in which a person has reduced sperm motility and so, the chance of the sperm fertilizing the egg in the female reproductive tract is reduced. This study aimed to investigate sperm DNA damage using SCSA and TUNEL tests in a large population of infertile men with asthenozoospermia, comparing them with a population of normozoospermic individuals with healthy sperm samples.

In this observational case-control study, semen parameters were assessed according to the World Health Organization guidelines (2010) guideline in a large population of asthenozoospermic (1383) and normozoospermic (2235) men referring to Isfahan Fertility and Sterility Center. In addition, sperm DNA fragmentation were assessed by TUNEL, and SCSA tests. For comparison of study variations between two groups, independent samples t-test was used, and for correlation between parameters, Pearson's correlation coefficient was carried out. P<0.05 was considered significant.

Despite no significant differences in sperm volume and body mass index, the means of sperm concentration, count, total sperm motility and total progressive were significantly lower in the asthenozoospermic men compared to normozoospermic men (P<0.001). In addition, the means of high DNA stainability as well as sperm DNA fragmentation assessed by SCSA and TUNEL tests were significantly higher in asthenozoospermic men compared to normozoospermic men (P<0.001).

In asthenozoospermic men, sperm DNA is severely damaged, so before using assisted reproductive techniques in these individuals, it is better to use antioxidant treatment and/or novel sperm preparation methods for treatment of them.

Objective:

 Infertility is affected by various factors. The hookah use can aggravate this problem. This study aims to determine the effect of hookah use on male infertility.

Methods :

This is a cross-sectional study on 40 men with hookah use and 40 non-users who referred to Imam Reza Hospital in Bandar Abbas, Iran in 2019. Data were collected using a researcher-made questionnaire, and semen parameters were analyzed. Independent t-test, Mann-Whitney U test, and Chi-square test were used to assess the relationship of hookah use with infertility-related parameters in abnormal and normal sperms of the two groups.

Results :

There was no significant difference between the two groups in terms of demographic parameters; hookah use was more in young and employed men with academic education. Means of sperm count,  motile sperm, sperms with progressive sperm motility, inactive sperms with progressive motility, and normal-shape sperms in the hookah users were 22±12, 35±10, 6±3, 21±6, 34±8 %, respectively, which were significantly lower than in non-users.

Conclusion:

 Given the adverse effects of hookah use on male fertility, health managers should increase the awareness of people about its effects.

The aim of the present study was to investigate the effects of endurance training and resistance programs on sperm parameters and spermatogenesis in diabetic rats.

In this experimental study, in one group, 24 healthy male Wistar rats were assigned into three subgroups (control, endurance training, and resistance training). The next group (n=24) consisted of male rats, in which diabetes was induced by intraperitoneal injection of nicotinamide (120mg/kg( + streptozotocin (65mg/kg( after 12 hours of starvation. This group was divided into three subgroups (diabetic control, diabetic endurance training, and diabetic resistance training). One week after the induction of diabetes, resistance, and endurance training protocols were performed in all groups for 10 weeks. Twenty-four hours after the last training session, the left epididymis was removed to examine sperm parameters.

Blood glucose levels significantly reduced in diabetic group with endurance and resistance trainings (P<0.0001). Sperm parameters (count, motility, and morphology) did not significantly increase in the diabetic group with endurance and resistance training programs compared with those in the diabetic control group (P<0.05). Spermatogenesis indexes (tubul differentiated index, spermiogenesis index, and repopulation index) significantly increased in the diabetic group with endurance and resistance training programs compared with those in the diabetic control group (P<0.0001).

Both types of exercises improved spermatogenesis indexes, but further experiments are needed to evaluate the role of moderate-intensity exercise on the oxidative stress in fertility of diabetic rats.

Infertility is one of the disorders, which its prevalence is increasing and several factors associated with reduction of sperm quality, can play an important role in this disorder. The aim of this study was to determine the correlation between dietary antioxidants intake and sperm quality in infertile men with oligozoospermia.
 
This cross-sectional study was conducted on 175 infertile men with oligozoospermia in Isfahan Infertility Center in 2018. In this study, a food frequency questionnaire with confirmed validity, was used to assess dietary intake. Sperm sample was collected with 3-day abstinence of sex, and two samples were taken for more assurance. Sperm parameters (concentration, motility, count, and morphology), were evaluated according to WHO standards. Linear regression analysis was used to determine the relationship between each antioxidant and sperm quality parameters. Dara were analyzed using linear regression and covariance analysis.
 
In this study, the mean age of the subjects was 32.19 years. The mean weight and body mass index of the individuals, were 85.28kg and 26.87kg/m2. There was a significant reverse correlation between beta-carotene and vitamin C intake with DFI. Also, there was a significant correlation between the intake of lutein and beta-cryptoxanthine with the count and concentration of sperm.
 
The findings of the present study indicated an inverse correlation between some of the antioxidants and related sperm parameters. Therefore, broader research with a higher target community, is required for further investigation.

Antioxidant alpha lipoic acid and β-defensin 1 protein may have a positive effect on the quality of sperm parameters. In this study, the effect of sperm incubation with alpha lipoic acid antioxidant and β-defensin 1 protein were assessed on sperm motility and viability at different times. In this experimental study, routine semen analysis was carried out on 59 infertile men according to the World Health Organization criteria (2010). Semen samples were processed by density gradient centrifugation. Then, samples were divided into three groups: control, processed semen sample treated with alpha-lipoic acid, and processed semen sample treated with beta-defensin 1. Then, all portions were evaluated for sperm motility and viability. The results were analyzed using one-way ANOVA. The mean percentage of motility in alpha-lipoic acid and β-defensin 1 groups after 1 and 2 hours, showed a statistically significant increase (p=0.05), while at the 3rd hour, only alpha-lipoic acid group had a significant increase in sperm motility. The mean percentage of sperm viability in alpha-lipoic acid and β-defensin 1 groups showed a significant increase compared to the control group after 1, 2, and 3 hours (p≤0.05). Although both alpha-lipoic acid and beta-defensin protein are effective for maintenance of sperm motility and viability after separation of time, it is recommended to use alpha-lipoic acid in sperm processing medium to maintain sperm motility and viability.

Today, we are dealing with the waves including, the waves emitted from wireless devices connecting to the Internet, which can have a different negative impact on human health. The aim of the present study was investigated the effect of electromagnetic waves emitted from the tablet connected to internet on the movement and number of sperm in adult male rat. 40 male rats were divided into two control and treatment groups with electromagnetic radiation emitted from a tablet connected to the Internet, respectively. Sperm count and sperm motility in both groups were analyzed by T-test. The results showed that the electromagnetic waves emitted from the tablet connected to the Internet caused a significant decrease in the total number of sperms and had a significant effect on sperm motility. In the treatment group with electromagnetic waves from tablet, the number of healthy and damaged sperm had significantly decrease and increase respectively compared to the control group, but there was no significant difference between semi motile sperms in the two experimental groups. It can be concluded that the electromagnetic waves emitted from the internet-connected tablet lead to reduced count and motility of sperm.

There are various techniques for treatment of male infertility, nowadays. At the beginning, evaluations are performed to determine the cause of infertility and the treatment. These include advanced molecular evaluations and assessment of sperm parameters. There is no study investigating the relationship between sperm parameters as an elementary index of male infertility, and DNA methylation as an important epigenetic mechanism with rat sperm ubiquitination, so far. The aim of this study was to evaluate the motility, morphology, DNA methylation, and ubiquitination in rat sperm and to determine the relationship between them.
First, 10 male mature rats were kept in experimental condition for 9 weeks (one cycle of spermatogenesis). After sacrificing, their semen samples were used to determine the sperm parameters and smear preparation. Through prepared smear and immunofluorescence assay, percentage of ubiquitinationed and methylated sperm were determined and finally, the correlation coefficient between them was calculated.
Findings: There was no significant correlation between the ubiquitination and DNA methylation. However, there was an inverse and significant correlation between the percentage of ubiquitination and morphological abnormalities in spermatozoa (P Ubiquitination, as one of the important molecular processes, prevents the participation of defective sperm in fertilization, and transmission of disorders to next generation. DNA methylation and ubiquitination affect sperm chromatin, but these two processes act in an independent manner.

Terfezia Boudieri (TB) has been used as a sexual stimulant for men in traditional medicine. TB containing fatty acid, flavonoids, beta-carotene, minerals and antioxidants such as, catechin, therefore, this study aimed to investigate the effects of the hydroalcoholic extract of Terfezia Boudieri on sperm and testosterone levels in male rats.
In this study, 21 adult male Wistar rats, each weighing approximately 250±20g, were randomly divided into three groups (n=7). The first group (control) with no treatment, the second group (sham group) received normal saline (extract solvent) and the third group, was injected intraperitoneally (IP) with 105 mg / kg of methanolic extract of Terfezia boudieri (TBME) (0.2 ml) for 21 days. The blood samples were collected to determine the concentration of testosterone and finally, the weight and size of the testicles and epididymis, the number and the percentage of sperm moving were evaluated Data were analyzed using ANOVA and Turkey's post- hoc tests.
The results of the present study showed that serum levels of testosterone, body weight of rat, testis weight , sperm count and sperm motility in the experimental group were significantly increased compared with the control group (p The results of this study showed that TB at 105 mg/kg dosage can increase the levels of testosterone and improve sperm parameters and therefore, TB can be used to treat sexual impotence and infertility in males.

Gold is a heavy, valuable metal with valance of three. The most important applications of gold are in jewelrymaking, dentistry, electronics, various catalysers, mintage, as well as sports and military medals. Researchers investigated the effects of age, nutrition, and chemical toxins on male fertility. This article aims to investigate some of the reasons behind the Islamic order concerning the decorative use of gold for men.
Material and

In this cross-sectional study, semen samples from 25-35 year-old males were collected and a standard questionnaire was used to record medical information of the participants. Then, Perkin-Elmer method (1973) was applied to assess the amount of gold in semen samples with an accuracy of nanogram by flameless atomic absorption using carbon rod atomizer.

Average gold content per unit of semen in non-goldsmith males was 92.2 ng/ml. The amount of gold content per unit of semen revealed a significant and logical relationship with percentage of natural motile and none-motile sperms. According to the data collected by the questionnaire, reduced fertility in male goldsmith population was significant.

The relationship between reduced sperm motility and increase in the amount of gold content per unit of sperm, in addition to the findings regarding a significant reduction in fertility rates among the jewelers of Tehran and Mazandaran provinces of Iran, may confirm the narratives of the Prophet Mohammad and the Shia Imams in prohibiting the use of gold ornaments or utensils for men.

Backgrounds & apium graveolens contains antioxidant activity and high level of polyphenolics. The purpose of this study was to determaine the effect of Apium graveolens seeds extract on semen parameters and serum testosterone level in mice. In the present experimental study, sixty male mice were divided into three experimental groups and a control group. The hydroalcoholic seed extract of Apium graveolenas L. was administered intraperitoneally at the doses of 200, 400 and 800 mg/kg for 14 days. A week after the final injection, blood samples were collected for hormonal assay. Then, the testes weight, sperm count and cauda epididymal sperm motility was assessed. Data were analyzed by ANOVA and Tukey's test. The results were compared with the control group indicating a significant increase in the total number of sperm at dose 400 mg.kg and increase sperm motility was seen in groups receiving 200 and 400 mg.kg respectively (P<0.001). Increased testosterone levels in the group receiving 400 mg.kg compared with the control group was observed (P<0.01). A significant increase was seen in testes weight compared with the control group (P<0.05). Apium graveolens seed extract appeared to be effective in improving semen parameters and serum total testosterones were dose dependent.

Varicocele is the most common cause of infertility in men. Increased release of free oxygen radicals is one of the most important mechanisms of damage in the pathophysiology of varicocele. The aim of this study was to determine the effects of saffron extract treatment on sperm parameters (sperm count, motility and morphology) in rats with experimentally induced varicocele. In this experimental study, 24 male Wistar rats were randomly divided into four groups: varicocele without treatment, sham, control, varicocele with treatment. Varicocele was induced in both varicocele groups. Saffron ethanolic extract (50 mg/kg) was daily administered intraperitoneally in the varicocele group with treatment for eight weeks. After eight weeks, the sperm parameters were studied in all groups. Data were analyzed using one way ANOVA. All sperm parameters in the varicocele groups were significantly reduced in comparison to the control group. The sperm parameters were significantly improved by saffron ethanolic extract. With regards to the results, crocin and safranal in saffron extract which probably eliminate free radicals can be effective in treatment of infertility caused by varicocele.

there is a great concern for the possible adverse effects of cell phones and noise waves and it looks these weaves effects on the individuals’ health. Thus, the present study was conducted to examine the effect of cell phone waves, severity noise, and simultaneous effect of cell phone waves and severity noise on sperm motility and sexual hormones in adult male rats. this experimental study was performed on twenty eight Wistar adult male rats (200-250 gr). The animals were randomly assigned to four groups (n=7): control group, two-week exposure to cell phone simulated waves group, exposure to noise group, and simultaneous exposure to cell phone simulated waves and sound group. The means of sperm motility in all groups were determined with criterions of WHO and sexual hormones concentration of blood plasma in all groups were determined by RIA. The results were analyzed by one-way ANOVA statistical technique followed by Tukey test using SPSS (version 16) software. sperm motility in the exposure to cell phone simulated waves and simultaneous exposure to cell phone simulated waves and noise groups decreased significantly compared to control group (P<0.05). The testosterone hormone concentration of blood plasma in all exposure groups decreased significantly compared to control group (P<0.05). exposure to cell phone waves and simultaneous exposure to cell phone simulated waves and sharp noise can cause a significant decrease in sperm motility and also, can cause a significant decrease the testosterone hormone concentration in adult male rats resulting in abnormality fertility.

It is known that concentration of docosahexaenoic acid (DHA) is reduced in the spermatozoa membrane of asthenozoospermic men. The aim of the present study was to investigate the effects of combined supplementation of DHA and vitamin E on changes in the serum and membrane fatty acids in asthenozoospermic men. This randomized، triple-blind، placebo-controlled clinical trial included 50 (from among 275) asthenozoospermic men with less than 50% sperm motility and less than 25% rapid progressive motility referred to Avicenna Infertility Clinic in Tehran، Iran. They were randomly assigned to one of two groups by stratified blocked randomization. Participants in the intervention and control group took، daily for 12 weeks، 465 mg of DHA plus 600 IU of vitamin E and two placebos، respectively. Sperm characteristics، serum and membrane fatty acid levels، dietary intakes، anthropometric measurements and physical activity were determined at the baseline and at the end of the study. Statistical analyses were performed using the SPSS software، the statistical tests being analysis of covariance، Student''s t-test، paired-sample t-test، repeated measurement، Wilcoxon، and Mann-Whitney tests. Out of the 50 participants entering the study، 22 in the intervention group and 20 in the control group completed the study. As compared to the control values، there were significant increases in the number of sperms (p<0. 001)، sperm concentration (p<0. 037)، percent of total motile sperms (p<0. 001)، percent of motile sperms with a progressive motility (p<0. 001)، DHA content of sperms (p<0. 001)، vitamin E concentration of serum (p<0. 001) and percent of DHA content of serum (p<0. 001) in the intervention group. The mean changes ( SD) in the total number of motile sperms and DHA content of the serum were 3. 60  2. 24 (p<0. 001) and 2. 88  0. 57 (p<0. 001)، respectively. Based on the findings، it may be concluded that combined supplementation of docosohexaenoic acid and vitamin E in asthenozoospermic men can lead to increases in sperm concentration of of docosohexaenoic acid and sperm motility. This fatty acid combined with vitamin E as an antioxidant may have a role in improving sperm motility.

Infertility treatment is important due to the high prevalence and destructive effects of psychological and social concerns of infertility. Intra Uterine Sperm Insemination (IUI) is a cost-effective and noninvasive treatment method of infertility. The aim of this study was to examine the relationship between sperm count, sperm motility, women's age and IUI success. This analytical study was performed on 55 couples, with 63 treatment cycles, in Kosar infertility center, Qazvin during 2009-2010. Basic information on women's age and their husband’s occupation was obtained through questionnaires. Sperm count and sperm motility were determined according to the WHO criteria. Sperm were prepared by swim up method and were transferred to the uterus with a catheter. After two weeks, the pregnancy was confirmed by urinary β-HCG test. Data were analyzed by Chi-square and Fisher exact tests and P≤0.05 was considered significant. The success rate was 9.5 % per cycle of IUI. No s ignificant difference was seen between the mean age of women in both successful and unsuccessful IUI groups. The relationship between the mean number of spermatozoa, the mean percentage of sperm progressive motility in the semen and pregnancy was not statistically significant. With regard to the results, women's age and percentage of sperm progressive motility could not be used to predict the success rate of IUI, accurately.

Considering the decrease of omega-3 fatty acids in spermatozoa of asthenozoospermic men, the aim of the present study was to investigate the effects of combined docosahexaenoic acid (DHA) and vitamin E supplements on spermatogram in asthenozoospermic males. In this randomized, triple-blind, placebo-controlled clinical trial, out of 275 men who referred to Avicenna infertility clinic, fifty asthenozoospermic males, defined as less than 50% sperm motility or less than 25% with rapid progressive motility, were randomly assigned to one of two groups according to the stratified blocked randomization. Participants in the intervention group took daily 465 mg of DHA and 600 IU of vitamin E; and those in the control group took daily two placebos for 12 weeks. Sperm characteristics, dietary intakes, anthropometric measurements and physical activity were measured at the baseline and at the end of the study. Statistical analyses were performed using the SPSS software, the statistical tests being analysis of covariance, Student's t-test, paired-samples t-test, Wilcoxon and Mann-Whitney. Out of 50 participants, 22 men in the intervention group and 20 men in the control group completed the protocol of the study. Number of sperms, sperm concentration, percentage of motile sperms and percentage of motile sperms with a straight direction increased in the intervention group, as compared with the control values (p<0.05). According to this research, combined DHA and vitamin E supplements led to increasing sperm concentration and sperm motility; however no significant changes occurred in sperm morphology and vitality in asthenozoospermic men. DHA and vitamin E, as an antioxidant, may improve sperm motility.

One of the causes of male infertility is the lack of proper mobility. One way to deal with this problem is to add adenosine to human sperms in the laboratory. Therefore, this study aimed to determine the effect of different doses of adenosine on sperm motility in the ejaculates of fertile and infertile individuals. In this experimental laboratory study, 60 samples of seminal fluid of infertile and fertile men who referred to Yazd Infertility Center were analyzed according to WHO guidelines. Sperm counts, morphology and motility (fast, slow and immotile) were studied. After using Swim-up technique, the washed samples were divided into 4 groups, containing doses of 0, 2, 5 and 10 mg adenosine. The sperm parameters were evaluated after the addition of adenosine. Data were analyzed statistically using paired T-test and independent T-test. The initial assessment indicated significant differences in sperm parameters, except for slow motility between the two groups. In fertile group, dose of 5 mg adenosine increased sperm count, and rapid motility, but lowered the rates of sperm immotility at doses of 5 and 10 mg. In the infertile group, sperm count improved at dose of 5 mg adenosine, but it showed no effect on slow sperm motility. 5 mg adenosine also improved rapid sperm motility, but dose of 10 mg had no effect. Both 5 and 10 mg adenosine significantly lowered rates of immotile sperms. Based on the results of this study, it can be concluded that addition of 5 mg adenosine to washed sperms can improve sperm motility of infertile men in vitro.