جستجوی مقالات مرتبط با کلیدواژه "تعلیق اندام تحتانی" در نشریات گروه "پزشکی"

Exercise training is employed as a practical method to combat muscle atrophy. The purpose of this study was to investigate the effects of high-intensity interval training (HIIT) conducted before and after hindlimb suspension (HLS) on the gene expression of MSTN and ActRIIB in the skeletal muscle of male Wistar rats.

Thirty-two male rats, aged four to six months, were randomly divided into four groups (N=8 each): (1) control, (2) training-suspension-retraining, (3) non-training-suspension-retraining, and (4) training-suspension-non-training. The HIIT duration was five weeks, while the HLS lasted for two weeks. Gene expression levels were assessed using RT-PCR.

A significant decrease in the expression of MSTN and ActRIIB was observed in the soleus muscle of the group (2) compared to the other groups (p<0.05). There was also a notable reduction in MSTN expression in the extensor digitorum longus (EDL) muscle among the experimental groups relative to the control group (p<0.05), with the group (2) showing a greater effect than the others. Additionally, a significant decline in ActRIIB expression in the EDL muscle of the group (2) was noted compared to the other groups (p<0.05). The group (3) also exhibited a significant decrease in ActRIIB expression in the EDL muscle compared to the control group (p<0.05).

The findings of this study suggest that the training-retraining approach (with HIIT) is particularly effective in mitigating muscle atrophy induced by HLS. These results highlight the HIIT training model as a highly efficient treatment method for preventing and reducing atrophy in rodent skeletal muscle.

Resistance training increases muscle mass and inhibits atrophy by changing the muscle mass and regulating the expression of muscle proteins. The purpose of this study was to investigate the effect of a detraining and retraining period on gene expression of ATF4 and P53 in the plantaris muscle of rats after hindlimb suspension.

32 male rats aged 4 to 6 months were randomly divided into four groups (N=8): 1- control (G1), 2- training-suspension-retraining (G2), 3- detraining-suspension-retraining (G3) and 4- training-suspension-detraining (G4). The duration of resistance training was four weeks and the duration of the suspension protocol was two weeks. At the end of the study, the animals were anesthetized using chloroform and dissected and their plantaris muscle was separated. The measurement of gene expression was done by RT-PCR. Anova and Tukey's test were used to determine the difference between variables.

A significant difference was observed between the research groups in ATF4 and p53 gene expression (F=16.57) (p=0.0001) and (F=55.07) (p=0.0001). In addition, there was a significant decrease in ATF4 and p53 gene expression in G2, G3 and G4 compared to G1. Also, ATF4 gene expression was significantly decreased in G2 compared to G3 (p<0.05).

Overall, the findings of the present study showed that resistance training is a very effective tool in preventing and controlling atrophy caused by immobility in animal model.