جستجوی مقالات مرتبط با کلیدواژه "روتین" در نشریات گروه "پزشکی"

Hepatic cholestasis is a primary cause of liver failure and can lead to complications in other organs, including the kidneys. Kidney damage resulting from liver dysfunction is known as cholemic nephropathy. Oxidative stress is a key mechanism in causing this damage. Oxidative stress occurs when the ratio of free radicals to antioxidant enzymes is imbalanced, with free radicals prevailing. Medicinal plants are often used to treat and prevent diseases due to their antioxidant properties. Rutin (ROT), a bioflavonoid with antioxidant properties found in certain foods, may offer protective effects. Therefore, this study aimed to investigate the protective effect of rutin on kidney damage following hepatic cholestasis in rats.

This experimental study included 50 Wistar rats, randomly divided into 5 experimental groups (n=10). Cholestasis was induced by ligating the common bile duct. Rutin was administered daily via gavage in different doses (30, 60, and 120 mg/kg) to the affected rats for 14 days. At the end of the intervention, the animals were anesthetized, tissue samples were collected, and serum and urine samples were obtained for further testing. Antioxidant enzyme activities, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), were measured in kidney tissue homogenates using an ELISA kit, following the manufacturer’s instructions. For assessing reactive oxygen species (ROS) and malondialdehyde (MDA) levels, 500 mg of kidney tissue was homogenized in 5 ml of cooled KCl solution. The protective effects of rutin were evaluated by analyzing serum biochemical factors (BUN and creatinine), urine parameters (glucose and protein), oxidative stress markers (ROS and MDA), and antioxidant enzyme activities (GPx, SOD, and CAT). Statistical analysis was performed using GraphPad software, with one-way ANOVA and Tukey’s test.

The study results showed that, following cholestasis induction, urinary biochemical factors (glucose and protein) significantly increased. However, rutin treatment significantly reduced urinary glucose and protein excretion (P<0.05). Additionally, in cholestatic animals, serum biochemical factors (BUN and creatinine) increased markedly compared to the control group, but following rutin administration, these biomarker levels significantly decreased compared to the cholestatic group (P<0.05). Oxidative stress marker analysis revealed that ROS and MDA levels were significantly elevated in the kidney tissues of cholestatic animals compared to controls; rutin treatment significantly reduced these levels in the treated group compared to the cholestatic group (P<0.05). Furthermore, GPx, CAT, and SOD enzyme activities were significantly reduced in the cholestatic group, but after rutin administration, these enzyme activities significantly increased compared to the cholestatic group (P<0.05).

The present study demonstrates that rutin administration, at various doses, had a beneficial effect in reducing serum and urinary factors and oxidative stress markers while enhancing antioxidant enzyme activity in kidney damage resulting from chronic liver failure.

 Aging is one of the important factors in the development of age-related diseases. Acrylamide can be produced during carbohydrate-rich foods prepared at high temperatures. Recently, studies showed that acrylamide can induce cellular senescence. On the other hand, Rutin as a natural flavonoid, has a potent antioxidant activity.

 This study aims to evaluate the ptotective effect of Rutin on oxidative-induced cellular senescence induced by acrylamide.
Methods NIH3T3 mouse embryonic fibroblast cells were used in this study, which treated by different concentrations of acrylamide and Hydrogen Peroxide (H2O2). Oxidative stress was assessed by measuring the malondialdehyde and glutathion concentrations. To evaluate the senescence process, β-galactosidase activity was measured by enzyme staining and β-galactosidase activity assay kit. The MTT assay was used to evaluate the cell viability. 

 Exposure of cells to acrylamide and H2O2 significantly reduced the cell viability, and Rutin significantly improved cell viability in cells treated by acrylamide (P<0.05). Rutin also increased glutathion level in cells treated by acrylamide and H2O2 (P<0.05). The rate of lipid peroxidation was significantly lower in the group treated by rutin and H2O2 than in the H2O2 group (P<0.05).The β -galactosidase activity was increased in the H2O2 and acrylamide groups. The use of rutin with acrylamide significantly reduced the activity of β-galactosidase enzyme compared to the acrylamide group (P<0.05). 

 Oxidative stress may be an important mechanism in acrylamide-induced  senescence in NIH3T3 cells. On the other hand, Rutin, as a potent antioxidant, can reduce cellular senescence by inhibition of oxidative damage.

In this work, a new method was developed for simultaneous determination of dopamine and rutin at glassy carbon electrode modified with CuO nanoparticles doped multi-walled carbon nanotubes (CuONPs–MWNT/GCE). This modified electrode has shown excellent electrocatalytic activity toward the oxidation of rutin in acetate buffer solution (pH=5). The modified electrode lowered the overpotential of the reaction by ~500 mV, and this advantage of modified electrode made it aproprate to measure trace values of dopamine in the presence of rutin. First, the electrochemical behavior of CuO nanoparticles, incorporated in the modified electrode, was studied. Then, the behavior of oxidation of rutin and dopamine at the modified electrode was investigated by differntial puls voltammetry. A linear calibration plot was obtained over the range of 0.05-580 µmol L–1 rutin and 0.09-600 μmol L–1 for dopamine. Detection limits of 0.013 µmol L–1 rutin and 0.021 μmol L–1 dopamine were obtained. The relative standard deviation of ten replicate measurements for rutin and dopamine was measured 1.2% and 1.53% respectively. The high sensitivity, low detection limit and reproducibility of the modified electrode, made the proposed electrode suitable for the determination of dopamine in the presence of rutin in pharmaceutical samples

Anxiety is one of the psychological problems before surgery. Providing information and training of patients are vital components of nursing care for reducing anxiety level. This study was performed aiming at comparing routine education and Video CD on anxiety level before general surgery. In this study, 60 surgery candidate patients were randomly assigned into three 20 person groups. All patients completed demographic data and Spielberger questionnaires in their admission day. Then, Patients’ education was performed by two methods of lecture and video CD in the day before surgery. Then, patients again completed Spielberger questionnaire two hours before surgery. Data were analyzed using t-, ANOVA, and Tukey tests. A p<0.05 was considered significant. The mean total score of anxiety in three groups, in the day before surgery had no significant difference (p=0.51), but, a significant difference was seen among the three groups, in terms of anxiety 2 hour before surgery (p<0.032). Also, Tukey test showed a significant difference between the education and control groups (p<0.01). The results of this study showed that both routine and video CD educations decrease anxiety level before surgery. It is suggested that these educations to be placed in nursing care before surgery.

Background and Despite routine episiotomy is questioned in specialized midwifery books for more than a decade, this method is still routinely performed in Iran. In addition to increasing health care costs it will increase the workload of nursing and midwifery. This study was done with the aim of determining the frequency of episiotomy and its related factors in a labor ward in Sari city. It was a descriptive cross–sectional study. The sample (n=915), women referred to Labor ward of Emam Khomeini hospital of Sari city in Iran were recruited by census during 2007. Data was collected using an information sheet including three parts of demographic characteristics of women, obstetrical characteristics and probable related factors of episiotomy, and the type of delivery (with or without episiotomy). Data was analyzed by descriptive statistics, c 2 and Mantel-Haenszel tests using SPSS-PC (v.16). Episiotomy was performed in 812 cases (88.7%). There was a significant relationship between episiotomy and maternal age (P=0.023), Parity (P=0.01), the first minute APGAR score (P=0.003), fifth minute APGAR score (P=0.000), and birth weight (P=0.000). There was no relationship between prolonged second stage, use of forceps and vacuum, neonatal head circumference, maternal literacy and residency. Regarding the routine use of episiotomy in some labor wards, and in order to physiologic management of labor and creating a good experience for mothers, it is suggested that episiotomy only be performed on the basis of indication. It will also reduce mortality, complications, and the health care costs and also nurses’ and midwives’ workload.