جستجوی مقالات مرتبط با کلیدواژه « شاخص های استرس اکسیداتیو » در نشریات گروه « پزشکی »

Paraquat is one of the chemical herbicides with high toxicity. In case of incorrect consumption, this chemical causes fatal poisoning in people, especially those active in the field of agriculture. Nowadays, using compounds of natural origin to improve or reduce the harmful effects of agricultural pesticides is one of the important issues that has attracted the attention of researchers in this field. Quercetin as a natural flavonoid is found in large quantities in fruits and vegetables. As a kind of natural antioxidant, this compound has great power in removing free radicals. The purpose of this research was to evaluate the protective effects of quercetin on ovarian toxicity caused by paraquat in rats.

To conduct this experimental research, 30 adult Wistar rats with an average age of 6 to 8 months and a weight range of 160 to 180 gr were randomly divided into 5 experimental groups including 6 rats in each group. Paraquat (1.25 mg/kg, intraperitoneally) was injected into mice. Then quercetin was injected intraperitoneally in concentrations of 50, 100, and 200 mg/kg. The negative control group received only normal saline. After 24 hours, the animals were sacrificed and their ovaries were evaluated for changes in oxidative stress indices.

The results showed that paraquat significantly increased the reactive oxygen species (ROS), protein carbonyl, and lipid peroxidation, and significantly decreased glutathione content and survival of ovarian tissue cells when compared with the control group (P<0.001). The results showed that different concentrations of quercetin could inhibit the lipid peroxidation reaction caused by paraquat toxicity, and the positive control group had a statistically significant difference compared to the negative control group (P<0.001). By administering quercetin in different concentrations, we saw a decrease in the production of lipid peroxidation. From a statistical point of view, quercetin at the concentration (mg/kg) of 50 had a significant difference with the positive control group (P<0.01) and this significant difference was also evident at the concentrations of (mg/kg) 100 and 200 (P<0.0001). Increasing the concentration of quercetin was associated with improved performance in inhibiting paraquat toxicity.

Results showed that quercetin can be useful as an effective antioxidant combination in improving oxidative stress indicators and ovarian tissue of mice. Quercetin can reduce paraquat-induced oxidative damage in rat ovarian tissues in a dose-dependent manner. Quercetin can potentially be used to control ovarian toxicity, modulate or inhibit oxidative factors, strengthen the immune system, and strengthen the antioxidant system in animal models. Probably, it can be used as a kind of safe supplement in combination with other drugs affecting the immune system. However, this hypothesis along with the effect of other drug interactions should be confirmed using appropriate in vivo studies. Understanding the process of cell death caused by paraquat and the inhibitory effect of natural antioxidants on it provides a new road map for future studies to develop new therapeutic strategies.

Diabetes mellitus, a metabolic disorder caused by a defect in the secretion or function of insulin. Arginine is an amino acid that is lower in these patients. Octopine is an arginine derivative. Therefore, the present study evaluated the effect of opine on glucose and stress oxidative indices in streptozotocin-induced diabetic rats.

In this study, 16 male mice were divided into two groups including diabetic and non-diabetic controls and 16 male mice in two experimental groups. The first experimental group received opine treatment at 50 mg and second experimental group received opine treatment at 100 mg of opine for 3 weeks. Diabetes was induced in diabetic rats through intraperitoneal injection of 60 mg/kg of Streptozotocin. Blood samples were enrolled from mice in fasting status and the level of malondialdehyde (MDA) was measured by thiobarbituric acid method, glucose levels using Pars-Azmoon Company Kit, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity using Randox (England) kits.

The opine consumption had no significant effect on serum glucose and MDA, while 50 mg opine treatment significantly increased the activity of SOD and GPX enzymes in comparison with diabetic rats.

The consumption of octopine had no improving effect on blood glucose levels but it improved antioxidant status in diabetic rats.

Not having physical activity with a physiological function disorder and reducing the body's resistance to oxidative stress. The green tea supplement increases plasma antioxidant defense system during rest and during exercise. The purpose of this study was to determine the effect of eight weeks of periodic aerobic exercise and green tea supplementation on oxidative stress indices in young girls inactive. The present semi-experimental study was carried out in the form of a pre and post design test. The statistical population consisted of all inactive young girls in Sanandaj city. 30 volunteers were randomly selected and divided into three groups of 10, including Practice-supplements, Practice-placebo and supplement alone. The periodic aerobic exercise program increased over the course of eight weeks, triple periods/week. Primary blood samples were taken at baseline before the start of supplementation, and the second blood samples were taken 48 hrs following the end of the 8-week activity. The Total Antioxidant Capacity (TAC) and Malondialdehyde (MDA) were measured using ELISA kit. One-way analysis of variance (ANOVA) with Tukey's post hoc test and t-test with significant level of p≤0.05 were used. There was a significant difference in MDA between the three groups. With MDA, the practice-placebo group showed a significant difference with the supplement group. However, in the TAC index, no significant difference was observed between the three groups in the post-test phase. Based on the results of t-test, the MDA index decreased significantly in all three groups, but the TAC index decreased significantly in the supplementation group. Regular physical activity seems to work like a supplemental anti-oxidant supplement which in stressful conditions maintains and improves the health of the person and addition of green tea supplement doubles the effect.

Zinc is part of the antioxidant system, so zinc deficiency impairs the body's antioxidant defense system and leads to increased oxidative stress. The objective of this study was to investigate the effect of zinc oxide on the antioxidant status in rat.
Twenty-five male Wistar rats of 5-8 weeks’ old were divided into five groups of five animals each. Next, during the following 10 days, four groups (1-4) of the rats were exposed to different doses: 50, 00, 150 and 200 mg/kg of zinc oxide (ZnO) as the intraperitoneal injection; and, group 5 was considered as the control group. After this stage, 2 ml blood was collected from the jugular vein of the rats and oxidant and antioxidant parameters containing glutathione peroxidase (GPX), superoxide dismutase (SOD), total antioxidant capacity (TAC) and lipid peroxidation (LPO) were assayed.
Activity of SOD increased significantly in the groups treated with different doses of ZnO (P The results of this study showed that the used concentrations of ZnO increased significantly SOD and TOS and reduced insignificantly GPX compared to the control group which can be a marker of oxidative stress induction.

Background and In recent years the role of nutrition and herbal medicine are considered in the control of cardiovascular risk factors. The aim of this review was to evaluate the effect of nigella sativa on glycemic indices, lipid profiles, markers of inflammation, oxidative stress, and anthropometric indices.
An electronic literature search was conducted on websites such as Science direct, Google scholar, SID, Iranmedex, and Scopus using the following keywords: nigella sativa, Timokinon, type 2 diabetes, insulin resistance, blood glucose, lipid profile, metabolic syndrome, body weight, inflammation, and antioxidant. Published articles (until August 2014) in English and Persian were selected.
Nigella sativa in various interventions improved insulin resistance, lowered blood sugar and HbA1c, increased insulin production, and decreased hepatic gluconeogenesis and glucose absorption in the intestine. Nigella sativa also showed improvements in lipid profile. It can improve the inflammatory status (by reducing the inflammatory cytokine and anti-inflammatory increase in the inhibition of NF-KB factor and Nitric oxide) and oxidative stress (by increasing the activity of antioxidant enzymes and reducing free radicals, such as Malondialdehyde) in treatment of diabetes. Nigella sativa modifies the metabolic syndrome associated with type 2 diabetes by improving anthropometric parameters.
Nigella sativa have a favorable effect on management of cardiovascular risk factors such as dyslipidemia, inflammatory markers, and oxidative stress.

Liver ischemia-reperfusion (IR) is one of the common consequences of liver surgery, especially during liver transplantation which results in organ dysfunction. Acute hepatic injury causes systematic inflammatory responses which may finally lead to functional disturbances in remote organs such as heart, lungs and kidneys. In this study, the effects of a potent inhibitor of inflammatory cytokines (pentoxyfilline, PTX) and a well known antioxidant, (N-acetylcysteine, NAC), was evaluated on renal functional damage and oxidative stress following liver IR. Five groups of six male rats were used. Group one was sham operated. In group 2, 90 min liver partial ischemia was conducted by a clamp around hepatic artery and portal vein and followed by 4 hours of reperfusion. In group 3 and 4, PTX or NAC was injected intraperitoneally before the ischemia, while in group 5 both drugs were co-administered. The levels of ALT, AST, ALP, BUN and creatinine in serum as well as MDA and GSH levels in renal tissues were measured. Significant increase in the serum levels of ALT, AST in IR group is indicative of liver functional damages comparing to sham operated rats. Elevated BUN levels and increased renal tissue MDA and decreased GSH levels in IR group demonstrates a significant kidney functional damage and oxidative stress comparing to sham group. Administration of PTX alone and PTX+NAC prevented the IR-induced increase in renal MDA levels. Administration of both drugs and their co-administration prevented the reduction in renal GSH levels. Pretreatment with PTX and NAC before liver IR induction may prevent renal oxidative stress by protection of cellular GSH concentration and a reduction in MDA levels.

Background and Paraoxon is the active form of parathion, which is an organophosphate pesticide (OP). The toxic effects of some OPs are not limited to inhibition of cholinesterase, they are capable to produce free radicals and induce disturbance in body antioxidant systems. The purpose of this study was to evaluate the effect of paraoxon on oxidative stress indexin the kidney of rat.Wistar male rats were randomly divided in four groups including: control (corn oil as paraoxon solvent) and three paraoxon groups receiving different doses (0.3, 0.7 and 1mg/kg) by intraperitoneal injection. 24 hours after injection, animal was given anesthesia and kidney tissue removed. After kidney tissue hemogenation, superoxidedismutase (SOD) and catalase (CAT), lactate dehydrogenase (LDH) and glutathione S- transferase (GST) activities, glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods.At doses higher than 0.3 mg/kg paraoxon, kidney SOD and CAT activities were significantly increased, comparing with the control, while GSH level was significantly decreased. There were no significant changes observed in GST, LDH activities and MDA levels.The results suggest that paraoxon induces the production of free radicals and oxidative stress. The enhanced activity of antioxidant enzymes in kidney of rats probably wasa function of the increased detoxification capacity. Depletion of tissue GSH is a prime factor, which can impair the cell’s defense against the toxic actions of free radicals.