جستجوی مقالات مرتبط با کلیدواژه "میکروپلیت دایلوشن" در نشریات گروه "پزشکی"

In this study, the effect of Myrtus extracts on 25 methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli ESBL strains isolated from patients were compared by two methods.

15 methicillin-resistant Staphylococcus aureus (MRSA) and 10 Escherichia coli ESBL isolates were used in this study. Fresh Leaves of Myrtus were collected from the herbal medicine farm. Extraction was performed using a reflux distillation. The effect of concentrations 0.195-100 micrograms per ml of Myrtus extract on clinical isolates was analyzed in disk diffusion method compared with micro broth dilution method and with MTT in 545 nm on an ELISA reader apparatus.
Findings: Inhibition zone diameter for the minimum effective concentration of 50 micrograms per milliliter in all isolates of ESBL and MRSA were as 8±1 mm and 11±1. Minimum Inhibitory Concentration (MIC) was 6.25mic/ml and Minimum Bactericidal Concentration (MBC) was determined 12.5mic/ml for E. coli ESBL. Furthermore, the amounts for MIC and MBC was determined as 12.5 and 25 mic/ml, respectively for Staphylococcus aureus.

The results of this study showed compliance of two methods in evaluation of drug-resistant clinical isolates. It was proved that the disk diffusion method could be determining range of effective concentration but micro broth method determines the effective concentration carefully. It is recommended that results obtained from disk diffusion not to be basis for final decisions in traditional medicine studies. Bacterial behavior in the broth and determination of the point of death greatly increases the accuracy of the results.

Hypercom perfuratum has medicinal properties in traditional medicine with antidepressant and anti-microbial effects. In this study, a new form of aqueous extract of Hypercom was prepared as a micro emulsion form, and its antimicrobial properties was investigated in comparison with ordinary aqueous extract on clinical strains of gram-negative bacteria.
Ten types of clinical strains were investigated in this work. Hypericum extract was prepared by water in boiling temperature and micro emulsion with the help of two types of emulsifiers. Antibacterial properties of the two materials were performed by determining the inhibition zone in a disk diffusion method and the measurement of minimum inhibitory concentrations (MIC) by the microplate dilution and a ELISA reader device.
Salmonella typhi has the highest zone of inhibition around 42 mm at a concentration of 8 mg/ml for extract and 30 mm in 0.1mg/ml micro emulsion but Pseudomonas had no zone. The MIC with microplate for Salmonella had greatest effect on the amount of 0.01563 mg/ml for Shigella concentration of 0.00625 mg/ml respectively. MIC for E. coli and Proteus extract 0.5 mg/ml and the microemulsion 0.025 mg/ml for both bacteria.
Comparison of Hypercom extract and its microemulsion form on gram negative bacteria confirmed that microemulsion in lower concentrations had the antibacterial effect equal to aqueous extract. The use of microemulsion of the extract was recommended in clinical study of herbal medicines.