جستجوی مقالات مرتبط با کلیدواژه "anti-proliferative" در نشریات گروه "پزشکی"

Rosemary is an aromatic plant with ancient and modern applications as a spice and herbal remedy. Due to the strong antioxidant potential of rosemary, the present study investigated the anti-proliferative and pro-apoptotic characteristics of rosemary on luminal A and triple-negative breast cancer cells. The effect of rosemary extract on the WNT10B and β-Catenin genes was also evaluated. The WNT10B and β-Catenin expression were measured by real-time PCR.  The outcomes of the MTT assay and AnnexinV/PI flow cytometry assay showed that exposure of MCF-7 and MDA-MB-231 cells to rosemary reduced cell viability in a dose-time-dependent routine and promoted apoptosis in breast cancer cells. It was revealed that the extract could exert cytotoxic and apoptotic effects by downregulation of WNT10B and β-Catenin. Our results suggest rosemary as a promising complementary herbal medicine for breast cancers, without the adverse effects of chemotherapy drugs.

Melanoma is the most serious kind of skin cancer which has significantly increased in recent decades, and the importance of its primary treatment is increased widely. Ficus carica leaves have various therapeutic impact such as anti-inflammatory, anti-proliferative and apoptotic activity.

Hence, regarding the F. carica effect on the treatment of various diseases, the present research was conducted to identify the effect of methanolic extract of F. carica leaf on apoptosis induction in B16F10 melanoma cancer cells.

Cell survival was estimated by MTT assay after treatment of B16F10 cells in various concentrations of F. carica leaf extract (150, 250, 350, 450, 550, 650, 750 and 850 µg /mL) for 24 and 48 h. Cell apoptosis was analysed by AO/PI and DAPI staining, Annexin V/Propidium Iodide flow cytometry. Moreover, Real-time PCR was utilized to evaluate the expression of apoptotic genes including p53, Bax, caspase-3 and caspase-9 genes.

MTT assay results indicated that methanolic extract of F. carica leaf prevented the proliferation of B16F10 cells in a dose and time dependent manner. AO/PI staining results showed an elevation in apoptotic cells in treated groups and DAPI indicated that F. carica extract resulted in chromatin condensation and fragmentation. Annexin V revealed the increasing percentage of apoptotic cells after treatment. In addition, the up-regulation of apoptotic genes confirmed the apoptosis inducing potential of F. carica leaves in B16F10 cells by Real-time PCR.

Thus, methanolic extract of F. carica leaves could be suggested as an effective anti-cancer agent for further studies on melanoma cancer.

Pistacia atlantica is one of the species of Anacardiaceae that grows in the wild in different regions of Iran. Traditionally, anacardiaceae family has antibacterial, fungicidal and cytotoxic properties. Therefore, the present study was designed to investigate the possible cytotoxic and anti-proliferative properties of Baneh gum. Cytotoxicity of the plant gum was determined using MTT assay on MCF-7 human breast cancer cells. The cellular makers of apoptosis (caspase3 and P53) and cell proliferation (Cyclin-D1) were evaluated by western blotting. Doxorubicin was used as anticancer control drug in combination treatment. The result showed that Baneh gum (100 µg/ml) significantly induced cell damage, activated caspase3 and increased P53 protein level. In addition, Cyclin-D1 was significantly decreased in gum-incubated cells. Furthermore, combination treatment of cells with Baneh gum (25 µg/ml) and doxorubicin (200 nM) produced a significant cytotoxic effect as compared to each drug alone. In conclusion, Baneh gum (100 µg/ml) has a potential pro-apoptotic/anti-proliferative property against human breast cancer cells and its combination with doxorubicin in low doses may induce cell death effectively and be a potent modality to treat this type of cancer.

Pentagamavunon-1 (PGV-1) is a curcumin analogue that shows cytotoxic activity invarious cancer cells. In this study, we evaluated the effect of PGV-1 on a highly metastatic breastcancer cell line, the 4T1 cells, as an anti-metastatic and anti-proliferative agent. Cell viability was evaluated using MTT assay; while cell cycle profile, apoptosisincidence, and ROS intracellular level were determined by flow cytometry. Cell senescence wasobserved under senescence-associated-β-galactosidase (SA-β-gal) staining assay. The expressionof matrixmetalloproteinase-9 (MMP-9) was determined using immunoreaction based-ELISA,while other proteins expression were detected using immunoblotting. Curcumin and PGV-1 showed cytotoxic effects on 4T1 cells with IC50 value of 50 and4 μM, respectively. The cytotoxic activity of PGV-1 was correlated to the induction of G2/M cellcycle arrest and cell senescence. Furthermore, PGV-1 increased the accumulation of intracellularROS level. We also revealed that PGV-1 bound to several ROS-metabolizing enzymes,including glyoxalase I (GLO1), peroxiredoxin 1 (PRDX1), N-ribosyldihydronicotinamide:quinone reductase 2 (NQO2), aldo-keto reductase family 1 member c1 (AKR1C1). As an antimetastaticagent, PGV-1 showed less inhibitory effect on cell migration compared to curcumin.However, PGV-1 significantly decreased MMP-9 protein expression in a dose-dependentmanner suggesting it still potent to inhibit metastatic cells. Overall, our findings suggest that PGV-1 is potential to be developed as an antiproliferativeand anti-metastatic agent.

Metformin specifically inhibits the regulation of cell proliferation and survival via several mechanisms. This study aimed to investigate the anti proliferative effect of metformin against different cancer cell lines, in order to delineate its specificity and elucidate its mechanism. The inhibitory concentration (IC50) of metformin against several cancer cell lines was calculated and statistically compared with the corresponding values of doxorubicin as a reference drug using One way ANOVA followed by Tukey Kramer post test. Metformin was effective against PC3>Caco>HeLa>Hep-2>A549>Hep- G2>MCF7 cancer cell lines. The potency of metformin as anticancer agent in reference to doxorubicin ranged between 1.1 and 8%. The anti proliferative effect of metformin was attributed to its reduction of the glucose uptake and oxidation, leading to alteration of cell metabolism. Its anti proliferative effect against PC3 cell line with a minor effect against MCF7 cell line indicates that metformin helps the patients with positive androgen rather than estrogen receptor cancer cells.

Curcumin is a polyphenol extracted from the Curcuma plant. Curcumin has been used widely in ayurvedic medicine for centuries; it has a variety of biological properties including anti-oxidant, analgesic, anti-inflammatory and antiseptic activity. Curcumin has shown anti-cancer activities through variety of biological pathways engaged in mutagenesis, oncogene expression, cell cycle regulation, apoptosis, tumorigenesis and metastasis. Curcumin has proved anti-proliferative effect in many cancers, and is an inhibitor of the transcription factor NF-κB and downstream gene products (including c-myc, Bcl-2, COX-2, NOS, Cyclin D1, TNF-α, interleukins and MMP-9). Furthermore, curcumin affects a variety of growth factor receptors and cell adhesion molecules involved in tumor growth, angiogenesis and metastasis. However, a limiting factor is its extremely low bioavailability which hinders its use as therapeutic agent. Therefore, many technologies have been developed to overcome this limitation. We summarize to develop curcumin delivery aims and increase solubility for improving curcumin bioavailability and anticancer potential for therapy.

Cancer, a major cause of mortality worldwide, is a group of diseases distinguished by uncontrolled growth and expansion of abnormal cells. According to American Cancer Society, melanoma, a kind of skin cancer, is one of the most prevalent cancers. The side effects of chemical treatment developed more demands on natural products. Flavonoids, polyphenol compounds, with anticancer and antioxidant activity attracted more attention to themselves.
Through this investigation the effect of myricetin on cell proliferation was determined by MTT (Methylthiazolyl diphenyl-tetrazolium bromide) assay. A375 cell lines were seeded in a 96 wells plate and were exposed to different concentrations of myricetin (10, 15, 20, 40, 60, 80, and 100µΜ). After considered times, the MTT solution was added, then the viability of cells was detected by measuring the absorbance on 570 and 630 nm.
Our finding showed that low concentration of myricetin (up to 25µM) has no toxicity effect. Also the result confirmed the IC50 of myricetin on melanoma cells for three ordered period (24, 48, 72 hours) as following: 50, 40, 35µΜ, respectively.
According to this research, myricetin has anti-proliferative effect on melanoma cells, which can be used as a therapeutic agent. We hope that this study could be used as a mile stone in future researches to acquire confirmative results.