جستجوی مقالات مرتبط با کلیدواژه "balb-c" در نشریات گروه "پزشکی"

Immunotherapy is a new strategy to prevent and treat cancers. Cancer-testis antigens has gained lots of attention as the target of anti-cancer immunotherapy. In this study, Brother of regulator of imprinted sites (BORIS) cancer-testis antigen was used to design a DNA vaccine using bioinformatics tools, and its efficacy was assessed in murine colon cancer model in Balb/C mice.

In our previous study, the immunodominant regions of the BORIS antigen were identified. They were used to design a DNA vaccine inside the pcDNA3.1 plasmid after appropriate modifications and reverse translation. The DNA vaccine was used to immunized BALB/c mice four times at weekly intervals. 14 days after the last injection, the levels of specific cytokines and antibodies were measured. Moreover, to evaluate the protective effect of the DNA vaccine, colorectal cancer cells (CT26) were injected into mice, and tumors’ growth progression evaluated for 28 days.

Vaccination with this multiepitope DNA vaccine increased the level of gamma interferon (IFN-γ) and interleukin 4 (IL-4) cytokines (P < 0.001) and specific antibodies [immunoglobulin G1 (IgG1), IgG2a, IgG total] (P < 0.001) compared to control groups [phosphate buffered saline (PBS) and pcDNA3.1-VAC]. Moreover, mean tumors volume significantly decreased (P < 0.001) in the DNA vaccine immunized group compared to control groups.

This study suggests that the use of a BORIS antigen-derived DNA vaccine can be used as a strategy to induce a significant anti-tumor immune response, and inhibit murine colon tumors growth.

Aims: Developing an effective vaccine against Human Immunodeficiency Virus (HIV) is necessary. The aim of this study was the immunological evaluation of HIV-1 VLP harboring MPER-V3 in BALB/c mice model.
In the present experimental research, presenting 40 female mice, which were between 6 and 8 weeks old, were used for immunization with VLP MPER-V3. The mice were divided into 8 groups and in each group, 5 mice were considered. Injections were performed three times at three weeks intervals and subcutaneously in a volume of 100μl per mouse. Two weeks after last injection, mouse blood samples were collected by retro-orbital bleeding and immune responses were evaluated in serum for levels of total IgG and splenocytes for cytokine assay, using ELISA method. The data analysis was performed, using Mann-Whitney test and one-way analysis of variance.
Findings: The level of total antibody production was very high in all groups that had VLP alone or with adjuvant immunity, having a significant difference with the control group (p VLPs can stimulate the humoral immune system in mice immunized with these particles alone or formulated with adjuvant. Also, the level of production of IL-5 in the presence of the vaccine candidate as VLP increased significantly.

Fenugreek (Trigonella foenum- graceum L.) is a medicinal and edible plant in the family Fabaceae. The aim of this study was the effect of hydro-alcohol extract of fenugreek seed on reproductive system of female Balb/c mice. The animals divided to five groups of ten members: control group, placebo and three experimental groups. At first all animals synchronized. The control group received no drugs, while placebo group received physiologic serum. The three experimental groups received i.p injection of 50, 100 and 200 mg/kg/2days extract for 20 days. After 10 injections, blood samples were taken from all groups, hormonal measurement, including FSH, LH, progesterone and estradiol were performed by RIA technique. The results are analyzed to use of analysis of unilaterally variance and Duncan’s test by SPSS version11.5 program. Then these groups compared with control group. Ovaries were sectioned and observed by light microscope. Results &Discussion The data presented in this study showed significant decrease in the level of LH and FSH, also significant increase the level of estradiol in the whole experimental groups, while the level of progesterone only increased in the experimental groups. The results of histological studies of sections showed significant decrease in the folliculogenesis in the three experimental groups. Also significant increase the number of corpus luteum in experimental groups. The degeneration of ovary was observed in experimental group (200 mg/kg/2days extract). According to the results of folliculogenesis the ovary tissue in high dosage that indicates anti-fertility effect in female mice.