جستجوی مقالات مرتبط با کلیدواژه "berberine chloride" در نشریات گروه "پزشکی"

 Diabetes is considered as a major risk factor for cardiovascular diseases. Aerobic exercises and consumption of herbal supplements are the main interventions in the control and treatment of type 2 diabetes. 

 The purpose of this study is to investigate the effect of eight weeks of aerobic exercise training with berberine chloride supplementation on the expression of PGC-1a and SIRT-1 genes in cardiac muscle in male Wistar diabetic rats.

 In an experimental study, 56 adult male Wistar rats were randomly divided into healthy control groups (C), diabetic control group (D), diabetic group + 15 mg/kg berberine chloride (D-B1) ), diabetic group + 30 mg/kg berberine chloride (D-B2), diabetic group + aerobic exercise (DT), diabetic group + aerobic exercise + 15 mg/kg berberine chloride (DTB1) and diabetic group + exercise Aerobic + 30 mg\/kg berberine chloride (DTB2) were divided. The training protocol consisted of eight weeks and 3 training sessions every week, which started with a speed of 10 m/min for 10 minutes in the first week and reached a speed of 18 m/s and a duration of 40 minutes in the eighth week. Berberine chloride supplement in two doses of 15 and 30 mg/kg was given by gavage to the animals daily for 8 weeks. One-way analysis of variance and Tukey's post hoc test were used to check the changes.

 The results of one-way ANOVA showed no significant difference in the expression of PGC1-a gene (P = 0.071) and SIRT1 (P = 0.135) between the research groups.

 According to the results of the present study, it seems that aerobic exercise and taking two doses of 15 and 30 mg berberine chloride supplements do not have a significant effect on mitochondrial biogenesis indicators. Considering the limited literature, other studies are recommended to investigate higher doses of berberine chloride in combination with different training methods.

A serious complication of diazinon is the occurrence of oxidative stress in the respiratory system. The combination of medication and exercise rehabilitation has not been studied. The aim of this study was to assess the effects of berberine chloride alongside resistance training on apoptosis and oxidative stress markers of lung tissue in male rats exposed to diazinon.

Forty-eight adult male Wistar rats were divided into 1: resistance exercise + berberine
chloride dose 2mg/kg + diazinon; 2: resistance exercise + berberine chloride dose 15mg/kg + diazinon; 3: toxic (diazinon 1.5 mg/kg); 4: resistance training + diazinon; 5: berberine chloride dose 2mg/kg + diazinon; 6: berberine chloride dose 15mg/kg + diazinon; 7: intact control and 8: normal saline. To induce oxidative stress, diazinon was injected intraperitoneally 1.5mg/kg. Berberine chloride was used as 2 and 15mg/kg through intraperitoneal injection for 4 weeks. Resistance training was conducted 3 sessions/week for 4 weeks including climbing a vertical ladder. Lung tissue was exposed to evaluate pathohistological test, apoptosis and oxidative stress markers.

Berberine chloride and exercise had a significant effect on decreasing ROS, MDA, caspase-3, and increasing GSH level, but no effect on the 8-OHDG. The exercise alone has no significant effect on ROS, MDA, 8-OHDG, caspase-3 and GSH.

Berberine chloride alongside sport rehabilitation should be used as an effective treatment on lung apoptosis and oxidative stress with acute poisoning of diazinon. The most important mechanism is the effect of improving oxidative defense and anti-inflammatory effects.

Diazinon is one of the most widely-used organophosphate pesticides in the world. This toxin enters the body in various ways and induces oxidative stress in various tissues. It has been proved that activation of Unfolded Protein Response (UPR) under oxidative stress is a steady mechanism for maintaining cell function and survival. Therefore, the present study aimed to review the effect of Resistance Training (RT) and Berberine Chloride (BC) on the apoptosis-related UPR signaling pathway in the hippocampus of diazinon-poisoned rats.

In this experimental study, 40 male Wistar rats weighing 250 ±50 g were randomly divided into eight groups of five rats of 1) diazinon + 2 mg/kg BC + RT, 2) diazinon + 15 mg/kg BC + RT, 3) diazinon, 4) diazinon + RT, 5) diazinon + 2 mg/kg BC, 6) diazinon + 15 mg/ kg BC, 7) healthy control, and 8) sham. The groups were treated for 5 weeks. At the end of the fifth week, ATF-4, ATF-6, and CHOP gene expression in hippocampus tissue were measured by quantitative real-time RT-PCR.

Diazinon significantly increased the expression of ATF-4, ATF-6, and CHOP in the hippocampus tissue of rats. Administrating 15 mg/kg BC with RT significantly decreased these genes, indicating a decrease in the rate of apoptosis in the hippocampus.

This study showed that RT and BC have a protective effect against diazinon-induced toxicity in the hippocampus.

Exercise and herbal medicine Berberine are known as anti-inflammatory agents. This study aimed to evaluate the effect of 4-weeks of endurance training and Berberine Chloride (BC) consumption on inflammatory factors and glycemic index in male wistar diabetic rats.

In an experimental trial, 36 male wistar rats divided into 6 groups of 6 rats including 1) control, 2) 15 mg/kg BC, 3) 30 mg/kg BC, 4) endurance training, 5) endurance training with 15 mg/kg BC and 6) endurance training with 30 mg/kg of BC. During 4 weeks, rats in groups 2, 3, 5, and 6 received BC by gavage at specified doses, and rats in groups 4- 6 also ran on the treadmill at speeds of 10-15 m/min for 10-30 minutes. For statistical analysis of data-independent sample T-test, two-way ANOVA were used (P-value= 0.05).

Training and BC significantly increased function of pancreatic beta cells and reduced FBS, TNF-α, and IL- 6 (P-value= 0.001); Training significantly increased VO2max and insulin; interaction of training and BC on an increase of VO2max and reduction of TNF-α were significant (P-value= 0.001) and 30 mg/kg BC reduced TNF-α and FBS much more than 15 mg/kg BC (P-value= 0.001).

It appears that Endurance training and BC can decrease glycemic index and inflammatory markers of diabetes and the effects of BC is dose-dependent, so that the 30 mg/kg BC is more effective rather than the 15 mg/kg BC.

One of the most common agricultural pesticides is organophosphorus pesticides. Diazinon is one of the most widely used organophosphorus pesticides used in the fight against pesticides in the country. Much research has recently been done on the impact of exercise on brain function and several biological mechanisms have been proposed for the effects of exercise and physical activity on brain function. It is said that resistance training increases neuronal plasticity due to an increase in insulin-like growth factor-1 (IGF-1). Research has shown that IGF-1 can cross the blood-brain barrier and play an important role as a neurotrophic factor, especially in the hippocampus, and activate various important central mechanisms related to cognitive processes. Exercise also affects the expression of angiogenic genes such as PDGF and VEGF.

We randomly divided 40 male Wistar rats weighing 250 ± 50 g into eight groups of five and treated them with diazinon, berberine chloride, and resistance training. The groups were treated for five weeks. At the end of the fifth week, the levels of IGF-1, PDGF, and VEGF gene expression in hippocampal tissue homogenates were measured using Quantitative real-time PCR. Data were analyzed using ANOVA and Tukey’s post hoc test.

The results showed that resistance training and berberine chloride significantly increased the levels of IGF-1, PDGF, and VEGF gene expression in poisoned rats.

Based on the results of this study, resistance training and berberine supplementation have significant effects on reducing the harmful effects of diazinon toxin (P < 0.05) and increasing IGF-1, PDGF, and VEGF gene expression in poisoned rats.

Diazinon is an insecticide from the organophosphorus group, which can increase the oxidative stress in the body. It has been established that training and antioxidant supplementation can improve oxidative stress system by decreasing malondialdehyde (MDA); meanwhile, increasing the activities of antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT).

The aim of this study was to investigate the effect of resistance training (RT) and berberine chloride (BC) supplementation on oxidative stress indices in the cerebellum tissue of diazinon-poisoned rats.

Fifty-six Wistar rats were divided into seven groups (n = 8), including resistance training (RT) (each session 2 sets of 6 repetitions, each one required 8 to 12 active movements with intensity of 10% - 50% of body weight, 3 sessions per week), BC2 (BC: 2 mg/kg), BC15 (BC: 15 mg/kg), RT + BC2, RT + BC15, diazinon, and control. MDA, SOD, and CAT were measured in the cerebellum tissue.

RT and BC significantly reduced MDA protein levels (P < 0.01), while increased SOD protein levels (P < 0.01) and CAT protein levels (P < 0.01) in the cerebellum tissue of diazinon-poisoned rats; RT with BC had interactive effects on decreasing MDA (P < 0.01), while increasing SOD (P < 0.01) and CAT protein levels (P < 0.01). Also, the consumption of 15 mg/kg BC rather than 2 mg/kg BC significantly increased SOD protein levels (P = 0.02).

It seems that RT combination with BC has Interactive effects on the improvement of oxidative stress markers in the cerebellum tissue of diazinon-poisoned rats