جستجوی مقالات مرتبط با کلیدواژه "body fluids" در نشریات گروه "پزشکی"

Welding fumes (WFs) contain heavy metals that can induce oxidative stress and health issues in welders. This review investigated the relationship between oxidative stress biomarkers and trace metals in welders’ bodily fluids.

Online databases such as Scopus, PubMed, Web of Science, Science Direct, EMBASE, and Google Scholar were reviewed, with a specific emphasis on the effects of metal fume exposure during welding. Specific keywords such as “welding fumes”, “metal fumes”, “antioxidant enzymes”, “biomarkers”, and similar terms were employed to search for articles published between 2004 and 2023. After the evaluation of article titles and abstracts, this study reviewed a total of 19 articles.

Studies suggest that welders experience oxidative stress due to changes in trace metals in their body fluids, affecting antioxidant enzymes and oxidative stress biomarkers. Elevated heavy metals in welders’ biological samples lead to oxidative stress and inflammation, even at low levels. Certain metals in blood and urine, such as lead (Pb), manganese (Mn), iron (Fe), chromium (Cr), and cadmium (Cd), positively correlate with serum superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels. There is also a significant positive correlation between serum/EBC MDA and blood/urine Pb, Mn, Cd, Cr, and Fe, indicating cellular damage, lipid peroxidation, and reduced antioxidant capacity. Additionally, welders may experience more DNA damage compared to non-welders.

Exposure to WFs significantly altered oxidative stress biomarkers in bodily fluids, underscoring the importance of the relationship between oxidative stress and trace metal imbalances in WF-related injuries. These factors could serve as valuable biomarkers for monitoring workers exposed to WFs.

 The surface properties of dental and orthopedic implants are directly related to their osseointegration rate. Coating and/or modifying the implant surface might reduce the time of healing. In this study, we aimed to examine the effects of a hybrid surface consisting of a brushite surface coating and cross-linked water-soluble eggshell membrane protein on the osseointegration of Ti screws under in vivo conditions.

  Twenty Ti alloy screws were implanted monocortically in anteromedial regions of New Zealand rabbit tibiae. Ten screws were untreated and used as controls. The remaining 10 screws were coated with calcium phosphate and following cross-linked with ostrich eggshell membrane protein. All rabbits were sacrificed six weeks after the surgery. Peri-screw tissues were evaluated by µ-CT, histological and histomorphometrical methods. 

 The μ-CT assessments indicated that the experimental group had significantly higher mean BSA and TbN than those of the control group (p ˂ 0.05). BV, TbSp, TbTh and BMD scores of the control and experimental groups were quite similar (p > 0.05). The vascularization score of the experimental group was significantly higher than the control group (4.29 vs. 0.92%). No sign of the graft-versus-host reaction was observed.

  Our findings reveal that coating Ti alloy implants with calcium phosphate cross-linked with strich eggshell membrane protein increases the osseointegration of Ti alloy screws by increasing the bone surface area, number of trabeculae and vascularization in the implant site.

Healthcare workers are exposed to certain infectious diseases through needlestick injuries and exposure to blood and other bodily fluids. This study aimed to determine the prevalence of needlestick injuries and blood and other body fluids exposure among healthcare workers at Namazi Hospital in Shiraz, Iran.

Methods & Materials: 

This cross-sectional study was conducted on 656 Healthcare workers, including students and staff in medical, nursing, laboratory, service, and operating room groups, at the Namazi Hospital, in 2019. A questionnaire with four parts, including demographics, questions about using sharps containers, needle recapping, masks, and glasses, and the experience of sharps injuries or blood and body fluid exposure and reporting them, was distributed using the quota sampling method. To analyze the results, the T‑test and Chi-square test were performed.

Nearly 75.2% of the participants always used sharps containers, and 21.7% never recapped syringes. Sharps injuries and blood and body fluid exposure occurred in 40.7% and 39.8% of the participants. Occupation (P<0.001), being a student (P: 0.048), and age (P: 0.008) were associated with sharp injuries. Occupation (P: 0.001), gender (P: 0.016), and age (P<0.001) differed in exposed and no exposed participants to blood and body fluids. The prevalence of reporting exposure was not significantly different between men and women, occupation groups, students and staff, and different ages (P> 0.05).

Hospital managers should provide effective educational interventions to protect against occupational hazard exposures. Also, they should provide protective equipment for Healthcare workers.

Early detection of malignancies in the serous fluids has been remained an issue. A classic diagnostic tool for the ascites and pleural effusions is cytologic study (morphology) with approximately 98% specificity for the detection of cancer cells. This study aimed to evaluate the diagnostic value of three complementary markers in the serosal fluids of patients with malignant cytology and suspected cases.

Seventy two patients with serosal effusion treated in three teaching hospitals were studied. The cases underwent a diagnostic workup to determine the pleural effusion malignancy and etiologies. Complementary markers, including CEA, CA15-3, and CA125 were measured in serosal fluids of three categories of benign, suspicious, and malignant. The study was carried out by Chemiluminescence immunoalayzer. The morphologies were re-evaluated by a consulting Cytopathologist.

Of 72 serosal fluid specimens, 41 (56.9%) were related to pleural effusion and 31 (43.1%) were related to ascites. The sensitivity of CEA, CA125, and CA15-3 biomarkers were 64, 84, and 68%, respectively, and the specificity of each test was 100, 86, and 96%, respectively. This was statistically achieved for the combination of the area of markers below the curve (AUC), 0.93 and 90% sensitivity and 91% specificity.

The results suggest that complementary CA125, CA15-3, and CEA markers assayed with well-developed immunoassay method might be useful in the differentiation between malignant and benign effusions while combined with conventional cytology. CA125 yielded a significant correlation between cytomorphology and biomarkers based on the correlation coefficient analysis.

Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors which take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples.

Application of CT- scanning image information and radiation physical characteristics of the biomaterials are two measurable assays for presenting modified cells.

This study presented that CT number (HU) and linear attenuation coefficient contain useful information which can be determined during usual CT scanning for the prediction of breast cancerous cells existence based on hemoglobin concentration.

This experimental study used breast phantom containing major and minor vessels with diameters of 10 and 5 mm, respectively. The major vessels are filled by water, fat, hemoglobin (Hb) as a normal and 4× concentration of hemoglobin (4×Hb) as a cancerous breast cells, then scanned by single slice CT (GE, Hi Speed) 120 kVp, 100 mA for the determination of linear attenuation coefficient (µL).

The CT numbers were for water (-7 to +7 HU), Hb (22±6 HU) and 4×Hb (80±4 HU). The difference between Hb and 4×Hb was significant (p <0.000). Minimum µL was 0.1190±0.00680 cm-1 for fat and maximum was 0.1449±0.00794 cm-1 for 4×Hb.

The study of CT number and linear attenuation coefficient of different concentration of Hb provides a possibility for early predicting of breast cancerous cells existence (4×Hb).

This study aimed to systematically review previous studies conducted in Iran on the prevalence of injuries caused by needlestick, sharp, and cutting instruments and exposure to blood and body fluids. In this systematic review, all published full-text articles authored by Iranian researchers in Farsi or English in the field of needlestick injuries among healthcare workers during the period 2001-2016 were examined. Searches were performed on Iranian databases such as Scientific Information Database (SID), Iran Magazine Information Bank (Mag Iran), Iranian Journals of Nursing (N Index), and Directory of Medical Sciences Articles (Iran Medex) as well as international databases including Google Scholar, ISI Web of Knowledge, Scopus, PubMed, Science Direct, and CINAHL. The prevalence of injuries caused by needles ranged from 10.0% to 84.29% in different studies. The prevalence of exposure to blood and body fluids ranged from 13.46% to 79%. The results indicated that almost half of Iranian healthcare workers during their daily work are at the risk of needlestick injury and exposure to blood and body fluids. Hence, research and needlestick injury reduction strategies should be emphasized, and modifications should be made to organizational factors such as the development of educational programs, prevention along with performance monitoring, engineering factors such as redesigning tools to promote safety, and behavioral factors such as behavior modification of capping needles.

In this paper, preparation, bioactivity, and osteoblast cell behavior of cortical bone derived nanobiphasic calcium phosphate (nano-BCP) are presented. The calcined bovine bone samples with the addition of di-ammonium hydrogen phosphate were heated at 700°C for 100 min, and thus nano-BCP with the composition of 63/37 hydroxyapatite (HA)/β-tricalcium phosphate (β-TCP) was produced. Scanning electron microscopy (SEM) images, energy dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) analysis of immersed samples in simulated body fluid (SBF) solution showed that a uniform layer was formed on the surface after 7 days with the chemical composition of HA. The results indicated that the nano-BCP sample developed excellent bioactivity after 28 days. The nano-BCP samples showed better cell proliferation compared to pure HA samples. After 7 days in cell culture, the prepared nano-BCP (HA/β-TCP) exhibited the maximum proliferation of the MG-63 osteoblast cells.

Occupational hazards, exposure to blood and body fluids (BBF) accidents and safety precautions constitute an important public health issue. We assessed the prevalence and determinants of exposure to occupational hazards among nurses, and their knowledge of occupational safety precautions. In a cross-sectional study, we surveyed 332 nurses working in 4 hospitals, Nablus, West Bank, Palestine, by a questionnaire. Bivariate analysis tested the associations between ever exposure and the high likelihood of BBF exposure and the independent socio-demographic and occupational variables. Binary logistic regression analysis was used to assess the associations between the same two exposures and selected independent variables (those significant in the bivariate analysis). Prevalence of ever exposure to BBF was 51.7%, and was associated with working in private and charitable hospitals (OR 2.62, 2.68, respectively), having 4–6 family members (OR 0.52) and “nursing” being as one's top career choice at university (OR 0.48). The prevalence of high likelihood of BBF exposure was 62.2%, and was associated with working in charitable and private hospitals (OR 7.81, 2.43, respectively) and “nursing” being as one's top career choice (OR 0.57). Regarding knowledge, most respondents believed it is necessary to enact laws and regulations regarding occupational safety precautions, reported the use of sharps containers, immediate disinfection after an accident, reporting an accident, and using personal protective equipment. Nurses had adequate knowledge of the risks of their hospital work. Nevertheless, they exhibited high prevalence of exposure to BBF accidents. Future studies are needed to re-evaluate existing occupational safety guidelines in hospitals, establish monitoring and evaluation protocols for health care worker's adherence to the guidelines, and institute well-defined policies for reporting occupational injury incidents so these can be handled appropriately.

Health care workers occupationally are exposed to blood and other body fluids which might increase their risk of acquiring blood-borne pathogens and psychological stress. The aim of the present study was to determine the lifetime occupational exposure to needlestick injuries and body fluids among doctors and medical students in Rafsanjan University of Medical Sciences (2009). In this descriptive study, 100 doctors and medical students were asked to complete a valid and reliable self-reporting questionnaire regarding their lifetime occupational exposure to needlestick and body fluids. Data were analysed using SPSS. T-test and Chi-square test were used to compare the groups. The results highlighted that 57 (60%) of the respondents had at least one lifetime occupational exposure to needlestick and 45 persons (48.4%) had exposure to body fluids. However, only in 30 (31.5%) cases the event was reported to the appropriate authorities. The highest number of needlestick injuries occurred within the emergency departments (n=29, 51%), during nightshift (n=29, 51%), and with suture needle (n=37, 65%). Occupational exposures to needlestick and body fluids among doctors and medical students in our study have occurred with a relatively high lifetime incidence. Improving medical attentions might have positive effects on decreasing the incidence and prevalence of these constant occupational threats.