جستجوی مقالات مرتبط با کلیدواژه "disk diffusion method" در نشریات گروه "پزشکی"

Diabetes is one of the most important metabolic diseases worldwide. Wound infections due to antibiotic resistant bacteria can cause lower limbs ulceration and amputation in diabetic patients.The present study was performed with the aim of the evaluation of antibacterial effects of cellulose disc from kombucha- on bacteria isolated from diabetic foot ulcers.

In this descriptive-analytical study,bacterial were isolated from diabetic wounds and identified based on biochemical and molecular characterization. Then the antibacterial effect of Kombucha cellulose layer wasevaluated on the isolates using disc diffusion (qualitative) and agar dilution (quantitative) methods, and the data was statisticaly analyzed.

The most frequency of pathogenic bacteria that isolated in the present study from diabetic wounds were included 56% Escherichia coli (E.coli), 22% Enterobacter cloacae (E. cloacae), 6% Citrobacter diversus (C. diversus), 4% for each of Enterobacter aerogenes (E. aerogenes), Citrobacter freundii (C. freundii) and Klebsiella pneumonia (K. pneumonia), and 2% for each of methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus (S. aureus). TheResults of antimicrobial effect of kombucha cellulose disc showed that the disc weighing 0.5 mg was effective on all bacteria during agar disk diffusion method and the largest diameter of the growth inhibition zone was related to MRSA (27.5 mm).The minimum inhibitory concentrations (MICs) of Kombucha cellulose layer were 12.5 mg/ml on MRSA, 25 mg/ml on S. aureus, 75 mg/ml on  E. aerogenes, C. diversus and K. pneumonia, 71.15 mg/ml on E.coli, 85 mg/ml on E.cloacae, and 100 mg/ml on C. freundii.

The findings of this study showed that the cellulose layer of Kombucha has excellent antibacterial effects against  infectious bacteria in diabetic wounds and can be used in various medical and therapeutic targets.

Urinary tract infection (UTI) is one of the most common bacterial infections in childhood that delayed diagnosis and treatment can cause irreversible complications. The aim of this study was to determine the prevalence of urinary tract infection in elementary school children in Khorramabad city, Iran. In this descriptive cross-sectional study, 460 female students of elementary and secondary schools of Khorramabad city were recruited after completing a questionnaire including symptoms of infection, medical and disease history, and urine culture. Antibiotic susceptibility pattern was determined by disk diffusion method accordance with Clinical and Laboratory Standards Institute (CLSI) instructions. Statistical analysis were performed by SPSS software. Of 460 students, based on urine culture results 12 (2.6%) had urinary tract infection. Asymptomatic bacteriuria in 5 patients (1.1%) and positive culture and clinical symptoms were reported in 7 cases (1.5%). Escherichia coli (83.3%) was reported as the main cause of UTI among the tested samples. The highest antibiotic susceptibility was related to imipenem (91.6%) and ceftazidime (83.3%). The prevalence of UTI, pathogenic microbial agents and sensitivity to antibiotics in different regions and periods has a significant difference, which can be due to different health levels, constant change in incidence of germs that cause UTI and various antibiotics resistance. Because almost half of the cases of urinary tract infection are asymptomatic, screening for primary school children is necessary.