جستجوی مقالات مرتبط با کلیدواژه « dorema aucheri » در نشریات گروه « پزشکی »

Herbal products are beneficial compounds with many applications in human life. In this study the chemical composition and cytotoxic activity of the essential oil of the aerial parts of Dorema aucheri were assessed.

The essential oil was extracted by hydrodistillation after drying the aerial parts of D. aucheri, collected from the mountains around Yasuj city in the South-West of Iran. The oil composition was determined by GC/MS. To evaluate in vitro cytotoxic activity, the apoptotic effects of the essential oil were investigated against SW48 and SW1116 colorectal cancer cell lines by (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium) bromide (MTT) assay and flow cytometry.

The essential oil yield was obtained 0.02% (W/W). Twenty-five compounds were identified in the oil, and the main constituents were caryophyllene (E) (31.29%), Phytol (14.92%), gurjunene (β-) (9.84%), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (8.7%), and n-hexadecanoic acid (8.09%). The MTT assay showed that the IC50 values of the essential oil for SW48 and SW1116 cell lines were 1.4 and 1.2 mg/mL, respectively. The results of flow cytometry showed that the essential oil significantly increased the apoptosis in SW48 cell line compared with the vincristine (P < 0.05). It also increased the apoptosis in SW1116 cells compared with the vincristine, but this difference is not significant.

The essential oil of D. aucheri consisted of high amounts of caryophyllene and showed significant cytotoxic effects against SW48 and SW1116 cancerous cell lines.

Antibiotics resistance and unpleasant side effects of AChE inhibitors have led to an increased interest in herbs as potential sources. Ferula aucheri (Syn: Dorema aucheri) an indigenous species of Ferula (Apiaceae) grows in Iran and is used as food and medicinal plant. The present study was aimed to identify the oil composition and evaluate antimicrobial and AChE inhibitory activity of flowering tops, fruits and roots.

The chemical composition of the oils was recognized by GC and GC‐MS. The antimicrobial effects were assessed on 12 microorganisms by disc diffusion and micro-well dilution methods and AChE inhibitory potentials by a modified version of Ellman's method.

Sixty five compounds were identified from different organs and the notable characteristics have been high amounts of sesquiterpenes. Germacrene B (14.96%) and β-caryophyllene (12.87%) were distinguished as major components of flowering tops. Cis-dihydroagarofuran (9.02%) and δ-cadinene (8.28%) were identified as the remarkable constituents of fruit. δ-cadinene (18.25%) and gurjunene (12.62%) were detected from the roots by high content. All volatile oils exhibited lower MICs on Bacillus subtilis, Klebsiella pneumonia, Shigella dysenteriae,and Salmonella paratyphi-A serotype compared with gentamicin. Root and fruit oils were more effective than gentamicin against Escherichia coli and flowering tops oils proved lower MICs versus Staphylococcus aureus. Fruits and root oils showed weak potency for inhibiting AChE with IC50 values 554.05±4.65 and 239.69±3.5 μg/mL, respectively and flowering tops exhibited moderate activity (179.06±4.3 μg/mL).

The findings demonstrated that F. aucheri essential oils possessed antimicrobial activities with inhibition properties toward AchE.

Aflatoxins are one of the highly toxic secondary metabolites, which are mainly produced by Aspergillus parasiticus. This species frequently cause of food and agricultural products contamination including cereals, peanuts, and crops in the field. During recent years, researchers have considered research on elimination of aflatoxin and antifungal effects of medicinal herbals, such as Alpinia galanga L and Dorema aucheri. In this study, the effect of A.galanga L and D.aucheri a natural compound was examined on Aspergillus parasiticus growth, aflatoxins production and the aflR gene expression.
Antifungal susceptibility A.galanga L and D.aucheri was performed according to CLSI document M38-A2. Quantitative changes in aflR gene level of expression were analyzed by Real-time PCR method.
Our result obtained that the MIC of extracts on A. parasiticus growth 250 mg/mL for D.aucheri and 800 mg/mL for A.galanga L. D.aucheri has antitoxic properties as well as its effective ability to decrease aflatoxin production. The level of aflR gene expression was decreased significantly after the exposure of fungal cell to D.aucheri extract, but A.galanga L didn’t have significant effect.
This research indicated that D.aucheri has antifungal effects more than A.galanga L. Due to our obtained result we can suggest that D.aucheri herbal extract may have antifungal potential in medicine or agriculture.

Dorema aucheri is a plant of Apiaceae family which is used widely in some states of Iran. Different extracts and essential oil of Dorema species contain flavonoids and cumarin compounds which have anti‑hypertensive, cholesterol‑ and triglycerides‑lowering properties. This study was undertaken to evaluate the genotoxic properties of hydroalcoholic and aqueous extracts of D. aucheri on human hepatoma cells using the comet assay method for safety evaluation.

In this method, after incubation of cells with different concentrations of extracts, cell suspensions were added to pre‑coated normal agarose slides. After lysis, electrophoresis and neutralization process, staining was done by ethidium bromide and comets were observed using a fluorescence microscope. Tail length, percentage of DNA in tail and tail moment parameters were measured.

Statistical analysis of the results demonstrated that concentrations more than 500 μg/ml of hydroalcoholic and aqueous extract of D. aucheri were genotoxic.

It can be concluded from the results that taking the concentrations less than these dosages of extracts are safe but more studies are required to determine genotoxic mechanisms of this plant.

Dorema aucheri is a plant that grows in Iran. In Persian it is called (Bilhar). This experimental study was carried out at Ferdowsi University of Mashhad in 2014. After collection and preparation of aqueous and ethanolic extracts of Dorema aucheri (Bilhar), The antibacterial activity of ethanolic and aqueous extracts of Bilhar was evaluated against 7 laboratory strains of microorganisms, including 4 Gram positive (Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus and Bacillus subtilis) and 3 Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris). Its effects against human pathogen microorganism were determined using “Spreading of the Extract on Medium Surface” and “Disk Agar Diffusion Method”, Minimal Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) were determined for this extract. Collected data were analyzed by SPSS software using one-way ANOVA. The zone of inhibition for the ethanolic extract varied from 8 mm for P. aeruginosa to 24 mm for S. pyogenes and from 7 mm for P. aeruginosa to 19 mm for S.pyogenes in the aqueous extract. The minimum inhibitory concentration (MIC) of the extracts ranged between 2 mg/ml and 64 mg/ml while the minimum lethal concentration (MLC) ranged between 4 mg/ml and 256 mg/ml. Among of tested strains, P. aeruginosa has maximum MIC and MBC. 30 and 40 mg/mL Concentrations of Redcurrant have significant antimicrobial effect on bacteria. Antibacterial effect of extracts was decreased with decrease of extract concentration in disk. According to result, ethanolic extract of Dorema aucheri have antimicrobial effect on growth of all of the strains exposed analyzes and antimicrobial effect of that was maximum on Gram-positive bacterum of S. pyogenes. P. aeruginosa showed the highest level of resistance against the aqueous and ethanolic Bilhar extracts. The present study demonstrated that the ethanol leaf extract of Dorema aucheri hold an excellent potential as an antibacterial agent.

The geographic map of cancer prevalence differs due to environmental and dietary factors in various populations. High prevalence of a number of cancers in some regions is thought to be attributed to local dietary habits. Dorema aucheri (Bilhar) is used commonly as an herbal medicine in some regions including Iran. The aim of this study was to evaluate whether Dorema aucheri has carcinogenic effects in albino mice or not. The Dorema aucheri leaves were extracted by Soxhlet method and were injected intraperitoneally and randomly into 28 healthy albino mice which were divided into seven groups. One was put aside as the non-injected control group. The second control group was chosen to be injected by a known carcinogen. Another group was injected by carcinogen and then, Bilhar extract. The left four groups were injected the extracts in a dose- dependent manner, increasingly in the range of 0.4 – 3.2mL/kg. Extract injections were repeated every 48- hour intervals for three times. Then, liver and serum samples were analyzed biochemically and pathologically. The pathologic and biochemical studies showed that the injection of plant extracts caused necrosis, inflammation of the liver tissue, cell proliferation, cholestasis, and there were significant increases in release of liver enzymes [ALP, ALT (SGPT) and AST (SGOT)] and bilirubin compared to the non-injected control group. The level of liver damage was dose dependent. Dorema aucheri has potential hepatotoxic capacities and possibly this may be related to the high prevalence of cancer in some regions of Iran.