جستجوی مقالات مرتبط با کلیدواژه « doxycycline » در نشریات گروه « پزشکی »

Adipose-derived Mesenchymal stem cells (ASCs) have garnered attention for their regenerative potential; therefore, their cellular senescence-related gene expression remains crucial in therapeutic contexts. Nowadays, combination therapies have shown promising results in reducing senescent cells. This study investigated the effects of vitamin C, doxycycline, and azithromycin co-treatment on the key cellular senescence-associated genes in ASCs.

Human ASCs were cultured and treated for 24 hr with vitamin C, doxycycline, azithromycin, and a combination of three drugs. Total RNAs were extracted, and the expression of p21, p16, Nanog, Oct4, and Sox2 genes was assessed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Additionally, cell cycle alterations were analyzed via flow cytometry after treatment with these compounds. 

Notably, vitamin C treatment resulted in a significant down-regulation of p21 gene expression (P<0.01), implicating the potential role of vitamin C in promoting cell cycle progression. Doxycycline treatment led to a significant up-regulation of p21 and p16 gene expression (P<0.05), as it has previously been shown to induce cell cycle arrest. Similarly, azithromycin treatment predominantly increased p21 expression (P<0.05). Besides, cell cycle analysis revealed that each compound had changed the distribution of cells across different phases of the cell cycle. 

The combined use of all three drugs yielded intricate interactions, suggesting a complex yet promising approach to future research. According to our findings, the major difference in the combination drug-treated group (VDA) can be explained by the neutralizing effect of these three components in the environment.

Doxycycline is commonly prescribed as the primary prophylactic treatment for women undergoing hysterosalpingography (HSG). This study aimed to compare the prophylactic effectiveness and safety of azithromycin and doxycycline in terms of treating infections caused by HSG.

This double-blind randomized clinical trial enrolled 107 women referred to Amiralmomenin Hospital in Arak, Iran, for HSG. After evaluation based on inclusion and exclusion criteria, the participants were randomly allocated to either the doxycycline or
azithromycin group. The primary outcome was the incidence of adverse effects. The patients were also followed up to determine PCR test results for the clearance of chlamydia infection.

The cumulative incidence of side effects was 1.85% in the doxycycline group, with adverse effects observed in only one patient. Conversely, no adverse effects were reported in the azithromycin group (P = 0.505). In the doxycycline group, one patient (1.85%) tested positive in the PCR test, while no positive PCR tests were recorded in the azithromycin group. Neither group exhibited cases of fever or required additional treatments. The results of statistical analyses did not reveal any statistically significant differences between the compared groups (P > 0.05).

A single dose of azithromycin could be considered interchangeable with a 1-week course of doxycycline as antibiotic prophylaxis in women undergoing HSG, as it provides similar effectiveness and safety.

Human adipose-derived mesenchymal stromal/stem cells (hASC) constitute an attractive source of stem cells for cell-based therapies in regenerative medicine and tissue engineering as they are easy to acquire from lipoaspirate, expansion, and genetic modification ex vivo. The combination of Pdx-1, MafA, and NeuroD1 has been indicated to possess the ability to reprogram various types of cells into insulin-producing cells. The aim of this study is to investigate whether MafA and NeuroD1 would cooperate with Pdx-1 in the differentiation of hASC into insulin-producing cells.

In this experimental study, we generated polycistronic expression vectors expressing Pdx1 and MafA/NeuroD1 with a reporter from a human EF-1α promoter using 2A peptides in a single tet-off lentiviral vector system. Briefly, hASC were transduced with the lentiviral vectors and allowed to differentiate into insulin-producing cells in vitro and in vivo. Thereafter, RNA expression, dithizone staining, and immunofluorescent analysis were conducted.

Cleaved transcriptional factors from a single tet-off lentiviral vector were functionally equivalent to their native proteins and strictly regulated by doxycycline (Dox). Insulin gene expression in hASC transduced with Pdx1, Pdx1/ MafA, and Pdx1/NeuroD1 in differentiation medium were successfully increased by 1.89 ± 0.39, 4.81 ± 0.98, 5.51 ± 0.63, respectively, compared to venus-transduced, control hASC. These cells could form dithizone-positive cell clusters in vitro and were found to express insulin in vivo.

Using our single tet-off lentiviral vector system, Pdx-1 and MafA/NeuroD1 could be simultaneously expressed in the absence of Dox. Further, this system allowed the differentiation of hASC into insulin-producing cells

Acne vulgaris is a common skin disease. Choosing an appropriate treatment modality is important. We compared benzoyl peroxide 5% gel vs. new topical dapsone 5% gel in combination with doxycycline to improve acne. In a clinical trial, 60 cases with acne vulgaris aged 18-25 years were divided randomly into two groups, DD (dapsone 5% gel plus oral 100 mg doxycycline) and BD (benzoyl peroxide 5% gel plus oral 100 mg doxycycline). Topical dapsone 5% gel was made for the first time at Guilan University of Medical Sciences. The lesion counts, side effects, and acne severity (GAAS) were examined at baseline, 2, 4, 8, and 12 weeks. Satisfaction and improvement were assessed after 12 weeks. The Mann-Whitney, chi-squared, Wilcoxon, and Friedman tests were used for statistical analysis in SPSS v. 21. Inflammatory and non-inflammatory lesions were similar between the groups. Lesions were reduced within groups (P > 0.05). GAAS scores were similar between groups but decreased in both groups after 12 weeks (P = 0.003). Side effects (especially skin dryness) were less in the BD group after 12 weeks (P = 0.017), though erythema and skin irritation were less in the DD group (P > 0.05). Both groups reported a similar improvement rate (85%). However, satisfaction was more in the DD group (78%). The new dapsone 5% gel seems to be as effective as benzoyl peroxide 5% in combination with doxycycline. Considering its good tolerability, safety, and acceptability, it is suggested as an appropriate treatment for moderate acne vulgaris. (Clinical trial number: IRCT2017072035195N1)

Alopecic and aseptic nodule of the scalp (AANS) is a rare entity characterized by the presence of sterile, culturenegative nodules/cysts and areas of non-scarring alopecia.

We describe a case on the vertex of a 26-year-old man with a two-week history of a nodular, moderately-fluctuant, alopecic lesion. The patient also had cutis verticis gyrata (CVG).

The aspirate culture was negative and histopathologic findings were nonspecific. We also performed a trichoscopy of the lesion. After eight weeks, the patient showed dramatic clinical response to doxycycline and a dose of intralesional corticosteroid.

This is the first report of the simultaneous occurrence of AANS and primary essential CVG. AANS is a probably under-reported cause of non-scarring alopecia with a rapid and dramatic response to non-surgical treatment and a generally good prognosis.

Removing the pathogenic microorganisms from the root canal system is the key to a successful endodontic therapy. This study aimed to evaluate the antibacterial efficiency of three antibacterial agents and a new combination against selected endodontic pathogens.

In this in vitro study, the efficacy of three different antibacterial agents namely clindamycin, metronidazole, doxycycline, and their com-bination (CMD) was evaluated against seven bacterial strains associated with endo-dontic infections to determine their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). selective media were used to culture Candida albicans (C. albicans), Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli), Enterococcus faecalis (E. faecalis), Streptococcus mutans (S. mutans), Bacillus subtilis (B. subtilis) subsp. spizizenii, and Actinomyces actinomycetemcomitans (A. actinomycetemcomitans). All the tests were repeated in triplicate. The MIC and MBC values were reported as mean ± standard deviation. Data were analyzed by the Shapiro-Wilk test, the Kruskal-Wallis test, and Wilcoxon signed-rank test (P<0.05).

The intergroup comparisons of MIC for clindamycin versus CMD (P=0.036), metronidazole versus CMD (P=0.016), and doxycycline versus CMD (P=0.016) demonstrated significant differences. No other significant difference was noted (P>0.05). Intergroup comparisons of MBC for clindamycin versus CMD (p=0.036), metronidazole versus CMD (P=0.022), and doxycycline versus CMD (p=0.016) demonstrated significant differences. No other significant difference was noted (P>0.05).

CMD showed superior antibacterial efficacy than each individual antibiotic, and can be used effectively against the abovementioned endodontic patho-gens for their predictable elimination during endodontic therapy.

The prevalence of Helicobacter pylori (H. pylori) in developing countries is 50.8%, with the highest occurrence presented in Africa (79.1%). It increases the risk of chronic gastritis, peptic ulcer, cancer of the stomach, and lymphoma. The effect of standard
treatment for H. pylori eradication is below 80%, and evaluation of alternative lines of treatment is needed. We aimed to compare the hybrid, reverse hybrid, and levofloxacin quadruple therapies as first-line therapy in Egypt.

This was a randomized interventional trial done in the clinics affiliated with the Internal Medicine Department. 330 individuals were selected according to the inclusion criteria. They were divided into three groups: group 1 (110 subjects who received a reverse hybrid regimen), group 2 (110 subjects who received a hybrid regimen), and group 3 (110 subjects who received a non-bismuth levofloxacin quadruple regimen).

Group 3 had a significantly lower eradication rate of 82.7% versus 92.7% and 91.8% in groups 1 and 2, respectively. There were non-significant differences in the incidence rates of adverse events among the three groups.

Both the reverse hybrid and hybrid groups had good eradication rates in the Egyptian population, but non-bismuth levofloxacin quadruple therapy did not obtain a sufficient eradication rate.

In this study, we evaluated the effects of nanofiber and film polymers with doxycycline for treating a wound in a diabetic rat model. 108 male rats were divided into six groups, the control group, the diabetic control, and the groups were diabetic rats receiving different wound dressing. At the 3rd, 7th, and 14th days, macroscopic/histologic imaging and tissue sampling were performed. Tissues were analyzed for IL-1β, TNF-α, IL-10, TIMP-1, and MMP-2 by using ELISA. Dressings of chitosan, polyvinyl alcohol and doxycycline increased the rate of wound closure, the volume of collagen, dermal, and epidermis; in addition, it increased the number of fibroblasts and basal cell epidermis cells, vascular length, and decreased the number of neutrophil cells. Inflammatory cytokines and MMP-2 were decreased, and anti-inflammatory IL-10 and TIMP-1 were increased. It was ultimately attained that the combination of chitosan/ polyvinyl alcohol /doxycycline could be a useful dressing for the healing of diabetic wounds.

Self-inflicted acne excoriée is often observed in patients with compulsive skin picking, posing a challenge for diagnosis and treatment as the exact psychiatric cause remains unknown. Studies have suggested that serotonin reuptake inhibitors such as fluoxetine may help in the management of this condition. To determine the efficacy and safety profile of fluoxetine in the treatment of acne excoriée and to study the psychological profiles of patients.

Sixty patients of either sex aged ≥16 years with acne excoriée were assigned to either Group A (n=30), which received oral fluoxetine (F) (20 mg/day) and oral doxycycline (D) (100 mg/day) with topical clindamycin (1%), or Group B (n=30), which received a placebo (P), oral doxycycline (100 mg/day), and topical clindamycin (1%). Patients were evaluated for standardized rating scales and followed up every two weeks for 12 weeks.

Female gender predominance was observed in both groups, with most patients exhibiting anxiety and depression. With each follow-up visit (2nd, 3rd, 4th, and 5th) in both groups (A and B), the mean AESI (91.4% vs. 26.7%), HAM-A (80% vs. 27.7%), MADRS (68.1% vs. 28.2%), YBOCS (98.27% vs. 15.63%), PGA (88.47% vs. 31.38%), and VAS (99.17% vs. 37.67%) scores decreased from baseline by the final visit, indicating improved patient conditions. Significant differences were observed between the two groups (p <0.001) in the overall response, indicating that fluoxetine was more efficacious than the control. No remarkable side effects were noted.  

Fluoxetine efficiently managed acne excoriée without remarkable side effects.

Self-inflicted acne excoriée is often observed in patients with compulsive skin picking, posing a challenge for diagnosis and treatment as the exact psychiatric cause remains unknown. Studies have suggested that serotonin reuptake inhibitors such as fluoxetine may help in the management of this condition. To determine the efficacy and safety profile of fluoxetine in the treatment of acne excoriée and to study the psychological profiles of patients.

Sixty patients of either sex aged ≥16 years with acne excoriée were assigned to either Group A (n=30), which received oral fluoxetine (F) (20 mg/day) and oral doxycycline (D) (100 mg/day) with topical clindamycin (1%), or Group B (n=30), which received a placebo (P), oral doxycycline (100 mg/day), and topical clindamycin (1%). Patients were evaluated for standardized rating scales and followed up every two weeks for 12 weeks.

Female gender predominance was observed in both groups, with most patients exhibiting anxiety and depression. With each follow-up visit (2nd, 3rd, 4th, and 5th) in both groups (A and B), the mean AESI (91.4% vs. 26.7%), HAM-A (80% vs. 27.7%), MADRS (68.1% vs. 28.2%), YBOCS (98.27% vs. 15.63%), PGA (88.47% vs. 31.38%), and VAS (99.17% vs. 37.67%) scores decreased from baseline by the final visit, indicating improved patient conditions. Significant differences were observed between the two groups (p <0.001) in the overall response, indicating that fluoxetine was more efficacious than the control. No remarkable side effects were noted.

Fluoxetine efficiently managed acne excoriée without remarkable side effects.   Keywords: acnevulgaris,fluoxetine, doxycycline

Scaling and root planing (SRP) for the treatment of periodontitis may be less effective in some patients. This study evaluated the effectiveness of local doxycycline as an adjunct to SRP among smokers with periodontitis compared to SRP alone in randomized controlled trials (RCTs).

For this systematic review and meta‑analysis, PubMed and Scopus databases were searched till November 2018 for English publications. RCTs that compared the effect of local doxycycline adjunct to SRP among smokers with periodontitis were selected. Patient characteristics, disease characteristics, and outcome data on clinical attachment level (CAL) and periodontal probing depth at 1, 3‑ and 6‑month follow‑up was extracted. Quality of selected studies was assessed by the revised Cochrane Risk of Bias 2.0 tool. Random effects model and trial sequential analysis were performed. GRADE approach was used to assess the quality of evidence.P > 0.05 was considered as statistically significant.

Five trials were included in the review. Local use of doxycycline as an adjunct to SRP was effective in gain of 1.1 mm (0.47–1.74, P = 0.091) in CAL at 6 months calculated from two studies. The evidence was of low quality, and at least a total of 866 patients are required for conclusiveness.

Local doxycycline as an adjunct to SRP significantly improved clinical attachment in smokers with periodontitis and can be recommended. Studies are required with long‑term follow‑up and patient‑related outcome data.

Prevention of inflammation in early stages will be useful in maintaining vitality of the organism. The objective of this study was to evaluate the effects of doxycycline (DOX) or meloxicam (MLX) monotherapy and combination therapy on the levels of inflammatory mediators in the brain tissues of rats with Escherichia coli lipopolysaccharide (LPS)-induced brain inflammation. Seventy-eight rats were divided into the following groups: control (n=6), LPS (0.5 µg/10 µl intracranial) (n=18), LPS (0.5 µg/10 µl intracranial)+DOX (40 mg/kg intraperitoneal) (n=18), LPS (0.5 µg/10 µl intracranial)+MLX (2 mg/kg intraperitoneal) (n=18) and LPS (0.5 µg/10 µl intracranial)+DOX (40 mg/kg intraperitoneal)+MLX (2 mg/kg intraperitoneal) (n=18) groups. Brain tissues were harvested from all rats in the control group and from six rats each in the four experimental groups at 1, 3 and 6 hr under anaesthesia. The levels of tumor necrosis factor α (TNFα), interleukin 4 (IL-4), IL-6, IL-10, IL-17, brain-derived neurotrophic factor (BDNF), matrix metalloproteinase 3 (MMP-3), tissue inhibitor of metalloproteinase 3 (TIMP-3) and cyclooxygenase 2 (COX-2) in the brain tissues were measured using ELISA kits with ELISA device. LPS administration increased proinflammatory cytokines (TNF, IL-6, IL-17), and MMP-3 levels and decreased anti-inflammatory cytokines (IL-10, IL-4), and BDNF levels. The lowest TNFα levels were detected in the LPS+MLX group (P<0.05). All the drug treatment groups showed decreased IL-17 and COX-2 levels compared to the LPS groups. DOX or MLX monotherapy exerts neuroprotective effects against brain inflammation by decreasing proinflammatory cytokine levels and by increasing anti-inflammatory cytokines levels.

Periodontal ligament fibroblasts (PDLF) play a key role in periodontal wound healing and tooth‑supporting structures. Various approaches have been tried to enhance the fibroblastic activity such as laser irradiation or doxycycline application. The current study explored the influence of laser irradiation and doxycycline application on human PDLF. The aim of the study was the effect of low‑level laser treatment and doxycycline application on the expression of collagen I and matrix metalloproteinase‑8 (MMP8) from cultured human periodontal ligament cells.

In this experimental study After preparation of human PDLF in three replications, they were divided into five treatment groups. The first group was day 0, which was used for standardization. The second group was the control group, which received no treatment within 4 days of the study. The third group was treated with doxycycline 30, µm cc daily for 4 consecutive days. The fourth group was treated with diode laser 2 J cm2 daily for 4 consecutive days. The fifth group was treated with both doxycycline and laser irradiation pertaining to the third and fourth groups. After 4 days of treatment, cells were tested for collagen I and MMP‑8 secretion through real‑time‑polymerase chain reaction and ELISA reader. The data were analyzed using the ANOVA and least significant difference pair tests ( P < 0.05 ).

Treatment of human PDLF either with diode laser or doxycycline reduced the secretion of MMP‑8 significantly. The maximum reduction was related to doxycycline application. Regarding collagen, I, only doxycycline application significantly increased collagen I secretion. Other groups showed no significant increase in collagen I secretion.

This study showed that treatment of human PDLF either with diode laser or doxycycline significantly reduced MMP‑8. Treatment with doxycycline significantly increased the secretion of collagen I.

The aim of this study was to compare the substantivity of chlorhexidine (CHX) and doxycycline bond to the dentin in diffusion disk method.

A total of 92 dentin disks were prepared from Bovine’s teeth root. After removing cementum and standardizing disks in weight and outer diameter, they were irrigated with sodium hypochlorite 2.5%, ethylenediaminetetraacetic acid 17% and sterile saline and sterilled in autoclave. Then, the first group (n = 36) were irrigated with chlrohexine 2% and the second group (n = 36) with doxycycline 100 mg/mL, each for a period of 10 min. The third group (n = 20) was considered as the control group. Samples were divided into four subgroups, which after 1 day, 3, 6, and 12 weeks were incubated in plates containing Muller Hinton agar broth and Enterococcus faecalis for a period of 48 h at 37°C temperature; then the diameter of the zone of inhibition was measured. The antimicrobial effect of irrigating solutions without binding to the dentin was also studied using the plate well method. One‑way and univariate variance tests as well as Post‑hoc Tukey were used for data analyses.

The diameter of the zone of inhibition of doxycycline group was significantly more than the CHX group from the beginning to the 3rd week (P < 0.005). After 3rd week, mean diameter in doxycycline group declined as compared with the CHX group up to 12th week (P < 0.005). The difference between means in two groups was not significant in the 3rd week (P = 0.87).

The results of this study show that CHX and doxycycline show antibacterial activity for 12 weeks; although after 3rd week, the substantivity of CHX was significantly greater than doxycycline. Both of these irrigants can be used in other pharmacological and medical fields whenever a long‑lasting antibacterial action is needed.

The antimicrobial activity of a wound dressing is a key factor for preventing and treating wound infection. The current study evaluated the physiochemical properties and antimicrobial activities of semi-IPNs (interpenetrating polymer networks) based on chitosan/polyvinyl alcohol (PVA) films and nanofibers as candidates for wound dressings and investigated the effects of morphologies (nanofibrous mats and films), crosslinking conditions of chitosan chains (uncrosslinked and crosslinked with genipin), and the presence of antibacterial drug (doxycycline) on their physicochemical and antibacterial properties. The morphology, chemical structure, fluid uptake, water vapor transmission rate, antimicrobial activity, and doxycycline release profile were assayed using SEM, FTIR spectroscopy, swelling test, permeation test, agar diffusion antibiogram, and dissolution test, respectively. The results demonstrated that crosslinking chitosan with genipin reduced the diameter of nanofibers, fluid uptake, and drug release from both nanofiber mats and film samples. According to the results, wound dressings with film morphology have better antimicrobial activity than those with nanofiber. The chitosan/PVA/Doxycycline 1% film has the potential for use as an antimicrobial wound dressing.

Acetaminophen (APAP) toxicity threatens human health due to increased mortality associated with its overdose. Doxycycline (DC) because of its properties such as antioxidant and anti-inflammatory can be a good therapeutic strategy to treat the acute toxicity induced by APAP. Male mice were divided to six groups in two periods of 3 and 24-h as normal saline, APAP 400 mg/kg, DC 100 mg/kg and groups treated by 25, 50 and 100 mg/kg DC just before APAP, respectively. At the end of the 3-h and 24-hour periods, the hepatic index, biochemical parameters including serum aspartate transaminase (AST) and alanine transaminase (ALT) activity and hepatic catalase activity, reduced glutathione (GSH) and malondialdehyde (MDA) levels in liver and histopathological changes were evaluated. The results indicated that DC had no apparent effect on the hepatic index but significantly normalized the level of biochemical parameters and reduced APAP induced liver damage. Overall, it be concluded that DC can inhibit or resolve harmful effects of APAP through antioxidant and anti-inflammatory properties. However, more studies are needed to understand exact mechanism of DC and its application for clinical use.

Antibiotics are commonly used in the treatment of acne vulgaris. Considering the rise of antibiotic resistance, alternative medications may be used in the main anti-acne armamentarium. The aim of this study was to investigate the efficacy of oral azithromycin in the treatment of acne vulgaris. Database searches were performed in PubMed and Scopus using the keywords "azithromycin" and "acne". Azithromycin 500 mg once daily for 3 days per week or in cycles of 10 days for 12 weeks are the most commonly used regimens. Available experimental data suggest that oral azithromycin is an effective and well-tolerated option for treatment of acne vulgaris.

Chlamydia psittaci is an intracellular and zoonotic pathogen that is transmitted through contact with birds. This pathogen causes various clinical manifestations, including subclinical cases and fatal cases. On the other hand, the variety and low specificity of many of the symptoms make diagnosis difficult while early detection of the disease causes a significant reduction in mortality. The patient was a 47-year-old man with a history of headache, fever, chills, dyspnea, and productive cough with a primary diagnosis of sinusitis, which was treated with co-amoxiclav. Due to the lack of recovery, the patient was hospitalized with the diagnosis of pneumonia and was treated with meropenem and levofloxacin. After showing no improvement and finding out about the history of contact with a parrot, the treatment was changed to Doxycycline and Ceftriaxone, which led to the reduction of symptoms. Eventually, due to the patient’s positive serological tests, the diagnosis of Chlamydia Pneumonia was established and the patient was discharged with oral doxycycline. As the fatality rate of psittacosis in absence of treatment is high, it is necessary to treat the patient immediately.