جستجوی مقالات مرتبط با کلیدواژه "e‑selectin" در نشریات گروه "پزشکی"

The surface of endothelial cells is covered with cell adhesion molecules including E‑selectin, intercellular adhesion molecule‑1 (ICAM‑1), and vascular cell adhesion molecule 1 (VCAM‑1) that mediate the adhesion and extravasation of leukocytes and play a pivotal role in inflammatory response. The aim of this study was to investigate the role of expression of adhesion molecules in inflammatory bowel disease (IBD) patients, irritable bowel syndrome (IBS) patients, and normal colonic mucosa.

IBS and IBD patients along with normal colonic mucosa were recruited in the study. In all groups, two biopsies were taken from each of the three anatomical sites (terminal ileum, cecum, and rectum). Three monoclonal antibodies, E‑selectin mAb, VCAM‑1 mAb, and ICAM‑1 mAb, were applied for immunohistochemical analysis.

In IBD patients, the expression of intensity of E‑selectin, VCAM‑1, and ICAM‑1 was found decreased, at least in cecum and rectum, in comparison with IBS patients and controls (P < 0.001, P < 0.005, and P < 0.007, respectively). Comparison of the expression of intensity of the aforementioned molecules in IBS patients and controls revealed significant augmentation at the cecum and rectum of IBS patients.

The expression of adhesion molecules appeared lower in IBD patients compared to IBS patients and controls. In addition, the expression of adhesion molecules appeared higher in IBS compared to the control group. Therefore, it could be hypothesized that the expression of adhesion molecules could be considered as an early event in the process of proinflammatory IBS group and may be other factors play a crucial role in the process of intestinal inflammation in IBD patients.

Hypercoagulable state is a common serious problem in patients with end‑stage renal disease (ESRD). ESRD patients are in a condition of chronic inflammation. An increased level of E‑selectin, “a key adhesion molecule that regulates leukocyte bindings to endothelium at damaged sites,” accompanies the higher risk of inflammation in ESRD patients. We aimed to investigate the possible correlation among E‑selectin as an adhesion molecule, coagulation factors, and inflammatory factors in children with ESRD.

Thirty‑five child patients with ESRD who had been on regular dialysis treatment were registered in our study. Nighteen sex‑ and age‑matched healthy volunteers were used as the control group. Laboratory tests were requested for the evaluation of hematological and biochemical parameters, and parathyroid hormone (PTH), and for coagulation state; fibrinogen, protein C, and protein S were measured. The enzyme‑linked immunosorbent assay (ELISA) (Biomerica, CA, and IDS, UK). for serum E‑selectin assay was provided by R and D Systems (Abingdon, UK).

Hemoglubolin (Hb), blood urea nitrogen (BUN), creatinine, calcium, PTH, triglyceride (TG) concentrations in serum as well as E‑selectin showed significant difference between the two study groups, as indeed was expected. Serum E‑selectin was significantly higher (P value = 0.033) in dialysis patients than in healthy subjects. E‑selectin was positively correlated only with phosphorus in ESRD children (r = 0.398, P = 0.018). No association was found for other parameters.

Although in our study circulating E‑selectin concentration “as an inflammatory maker” is independently positively associated with limited blood markers, for better evaluation, well‑designed cohort studies should be examined in ESRD children.