جستجوی مقالات مرتبط با کلیدواژه "gas chromatography mass spectrometry" در نشریات گروه "پزشکی"

 Olax subscorpioidea is a medicinal plant that Africans use to treat numerous ailments, including cancer. This research examines the antioxidant, anticancer, and in-silico properties of ethyl acetate fraction of Olax subscorpioidea’s (OSEA) on 7,12-Dimethylbenz(α) anthracene (DMBA)-induced cell proliferation in female rats.

Forty female Sprague Dawley rats averaging 110 ± 20 g were induced proliferation with DMBA (80 mg/kg) and treated with ethyl acetate fraction (250 mg/kg BW) of O. subscorpioidea or tamoxifen (6.6 mg/kg BW) before and after induction. The trial lasted 22 weeks. In-vivo antioxidant parameters such as superoxide dismutase (SOD), malondialdehyde (MDA), and reduced glutathione (GSH) were examined. Likewise, carcinoma antigen marker (CA153), and DNA methyltransferase 3-like (DNMT3L) activity were measured. Gas chromatography-mass spectrometry (GC-MS) detected the bioactive compounds, and molecular docking studies predicted the mechanism of action of OSEA against DNA methyltransferase.

Treatment with OSEA significantly increased the SOD activity, enhanced GSH levels, and lowered the levels of MDA, CA-153, and DNMT3L in DMBA-exposed rats. The GC-MS analysis of OSEA revealed the presence of 40 bioactive compounds. The molecular docking revealed that 4-cyclopentene-1,3-dione (-6.407 kcal/mol), 2-(2-hydroxyethylthio) (-4.926 kcal/mol) and 3,4,5,6-tetrahydrophthalic anhydride (-6.16 kcal/mol) had the lowest binding energies against DNMT1, DNMT3A, and DNMT3B, respectively. 2-(2-hydroxyethylthio) was the least toxic. The molecular dynamic simulation revealed that the interaction between DNMT3A and 2-(2-hydroxyethylthio) propionic was stable to an extent.
The in-silico and biochemical analysis of the ethyl acetate fraction of O. subscorpioidea showed that it can protect against lipid peroxidation and oxidative stress and may be a potent source of drug that serves as an effective therapeutic in the future.

Muscari Miller. (Asparagaceae family) contains about 50 species worldwide, which are distributed in the Central and Southeastern Europe, Southern Russia, Africa, and some area of Asia such as Iran, Iraq, Afghanistan, Anatolia, and Syria. This study was designed to assess the antioxidant and antimalarial activities of Muscari inconstrictum Rech. f. seeds as one of the Iranian species of Muscari genus. In addition, preliminary phytochemical analysis of the extracts with different polarities was performed.

The essential oil of M. inconstrictum seeds was prepared using Clevenger and extracted with n-hexane, chloroform, and methanol (MeOH) by Soxhlet apparatus. Gas chromatography-mass spectrometry (GC-MS) was used for the characterization of essential oil. Total phenol and flavonoid contents were measured using Folin–Ciocalteu and aluminum chloride reagents. Free radical scavenging and antimalarial activities were investigated via 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and cell-free ß-hematin formation methods.

GC-MS analysis of the volatile oil of seeds demonstrated the presence of sesquiterpenoid, alkanes, fatty acid, and linear alcohol structures as the main constituents. Among different extracts of M. inconstrictum seeds, the methanolic extract showed significant antioxidant activity, which can be related to the presence of flavonoid and other phenolic structures. Furthermore, chloroform extract is introduced as the most potent antimalarial part.

It seems that further studies on the M. inconstrictum seeds are necessary to focus on pure compounds and their biological activities.

To study the anticancer activity of Plantago major, we assessed the effect of ethanolic, methanolic and acetonic extracts of this plant on HCT-116, SW-480, and HEK-293 cell lines as control.

The cytotoxic activity, biocompatibility, and toxicity were evaluated by MTT assay, hemolysis, and Artemia salina-LD50 (on mice) tests, respectively. The analysis of the extracts of compounds was performed by GC-MS analysis.

The results showed that all the extracts had the most antiproliferative properties on the HCT-116 cell line. The root extract of P. major was more effective than the aerial parts, and IC50 values of ethanolic, methanolic and acetonic root extracts were 405.59, 470.16, and 82.26 µg/mL, respectively on HCT-116 cell line at 72 h. Hemolysis degree of the ethanolic extract of P. major aerial and root parts were approximately 1% at 400 μg/mL. No significant interference of the RBC was observed. The ethanolic extracts, the Artemia survived every concentration used, and no toxicity was observed. One week after the oral administration of different parts of P. major extracts, none of the mice died, even those were preserved with 2000 mg/kg. These results of GC/MS analysis showed that P. major extracts contain potential anticancer compounds, such as stearic acid (8.61%) in aerial parts of methanolic extract and 1,2- Benzenedicarboxylic acid, mono(2-ethylhexyl)ester (88.07% and 40.63%) in aerial and root parts of acetonic extract of P. major.

Our findings suggest that the P. major is a source of important pharmaceutical compounds with antiproliferative properties.

Over the years, management of human pathogenic microorganisms has primarily relied on the use of synthetic antibiotics. In the recent past, Tagetes patula essential oils (EOs) and their phytochemistry and bioactivities have received great attention in research. Purpose and

In this study, the component, antimicrobial activity, and antioxidant capacity (ferric-reducing antioxidant power assay) of EOs from five plant parts (shoot at vegetative growth stage [TPSV], shoot at flowering growth stage [TPSF], flower [TPF], fruit [TPS], and root [TPR]) of T. patula were investigated. The antibacterial activity against five gram-negative bacterial isolates (including Serratia fonticola, Klebsiella pneumoniae, Acinetobacter baumannii, Proteus mirabilis, and Escherichia coli) and five gram-positive bacterial isolates (including Staphylococcus aureus, S. epidermidis, S. saprophyticus, Streptococcus agalactiae, and Streptococcus oralis) was studied using broth microdilution method. FRAP assay was also used to evaluate their antioxidant activity.

One hundred and twenty-five compounds of the total EOs were identified, constituting a mixture of oxygenated monoterpenes (33%), monoterpene hydrocarbons (25%), oxygenated sesquiterpenes (19%), sesquiterpene hydrocarbons (12%), and furanocoumarins (8%). In this paper, for the first time, more than 60 new compounds were isolated from T. patula such as bergapten, sylvestrene, (E)-β-farnesene, (E)-epoxy-ocimene, (Z)-jasmone, γ-gurjunene, and γ-himachalene. The EOs of T. patula showed potent antibacterial activity against the studied bacteria with the highest growth inhibition observed in E. coli after 24 h of incubation (MIC value 0.08 and MBC value 0.32 µL/mL). The TPS-EO had the highest mean value for ferric-reducing ability at the three test times, whereas TPR-EO had no activity.

It was concluded that the potential biocidal activity of T. patula EOs could be substantially associated with their oxygenated constituents or the synergistic activity of their major and minor chemical components.

Propolis as a natural product has shown beneficial effects on human health. This study was aimed to investigate the chemical compositions and biological activity of three different extracts of propolis from two distinct geographic areas in Iran.

The chemical composition of Iranian propolis extracts that were collected in the Spring of 2016 from two provinces in northern Iran: Ardabil and Polur in Mazandaran Province were measured through gas chromatography-mass spectrometry (GC-MS) methods. In addition, antimicrobial activity and cytotoxicity effect on HN5 and LNCaP cell lines were evaluated. The data were analyzed using one-way ANOVA and p<0.05 was considered as significant.

The GC-MS analysis identified the presence of compounds that belonged to the different groups such as aromatics acids and their related esters, flavonoid and flavonoid derivatives and terpenes. Flavanone was the most dominant compound of flavonoids. The maximum growth inhibition was observed against S. aureus of ethanolic extract of propolis (p<0.05). Moreover, cytotoxicity showed that ethanolic and dichloromethane extracts had more inhibitory effects on cell lines than the water extract.

The results determined that extracts had the highest percentage of flavonoids. Therefore, it is expected that the synergistic effect of the main components of propolis is related to the increase of biological activity of propolis.

Rose water is the known product of distillation process of Rosa damascena Mill. It is considered as relaxing and calming agent in religious ceremony as well as favorable food and beverage additive. Rose water contamination can limit its quality and applications. In this study, the combination of ultrafiltration and ultraviolet treatment (F-U treatment) was used to reduce rosewater contamination in traditional and industrial rosewater products. The compositions of essential oils of samples were also analyzed by gas chromatography–mass spectrometry (GC/MS) before and after treatment. The results demonstrate that F-U treatment was able to reduce microbial load in all samples without any significant effects on essential oil constituent. GC/MS analysis shows that phenethyl alcohol and citronellol are the main compounds identified in samples before and after treatment. Consequently, application of ultrafiltration and ultraviolet (UV) radiation not only can diminish microbial contamination but also do not decompose the chemical compounds of samples. On the basis of these results, the combination of ultrafiltration and UV radiation beside other physical methods may be valuable alternative technique to obtain high-quality rose water with preserving its constituents, which may be lost during traditional thermal methods.

The organophosphorus pesticides are still applied in some developing countries including most regions of Iran. It is important to make sure that the level of pesticide residues at the time of harvesting vegetable crops (e.g. tomato and cucumber) is assessed in order to control that their levels are within the permissible limit in the domestic as well as overseas markets; and most importantly the governments should ban the application of such compounds. Regarding the increasing consumption of pesticides in agriculture, the current study aimed at determining the amount of pesticide residues in crops such as tomatoe and cucumber to ensure that Codex maximum residue limit (MRL) alimentarius is not violated.

A simple multiresidue method, using gas chromatography-mass spectrometry (GC-MS) and solid phase extraction, was employed to detect the pesticides with a sufficient extraction method.

The lowest level of pesticide residues were concerning carbaryl, fenpropat, and endosulfan in cucumber samples selected from greenhouses and fields with 11.1%, while the highest level was concerning diazinon in cucumber samples of the fields with 55.5%. Carbaryl and permethrin showed the high residue means of 0.37 and 0.72 µg/g in tomato and cucumber samples, respectively.

Vegetables and fruits with a high level of pesticide residues, which particularly violated the MRL, are sold in markets of Tehran, Iran. Therefore, it is suggested that the application of pesticides on fields and greenhouses be controlled and their residue levels be measured using the multiresidue method before distribution. The current study findings showed that the multi-residue method with GC/MS could detect a large number of pesticide residues at the same time with high accuracy. Therefore, it is recommended that this method be employed for all vegetables and fruits in order to assess their pesticide residue levels before reaching the markets