جستجوی مقالات مرتبط با کلیدواژه « il-8 » در نشریات گروه « پزشکی »
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Background
Although the role of B cells in normal pregnancy has been recently highlighted, their importance and function are not completely clarified. Until now, some investigations have shown that during pregnancy, regulatory B cells (Breg), a subset of B cells, are one of the key players in immune regulation by both producing IL-10 and cell-cell interactions. Therefore, any decrease in the number or function of these cells may lead to recurrent pregnancy loss (RPL). Thus, the objective of this study was to characterize Breg cell frequency and function in women who suffered from RPL in comparison with healthy non-pregnant and pregnant women (under twenty weeks of gestational age) as controls.
MethodIn this study, peripheral blood samples of women suffering from RPL (n=8), women with normal pregnancy under 20 weeks of gestational age (n=14), and healthy nonpregnant women (n=10) were collected. The frequency of Breg cells (CD19+CD24hiCD38hi) was measured by flow cytometry. The serum level of the IL-10 cytokine, as a marker of Breg cell function, was measured by ELISA.
ResultsThe Percentage of Breg cells in women who suffered from RPL was significantly lower than that of women who had normal pregnancies (P=0.0016). The percentages of Breg cells in women who suffered from RPL were also significantly lower than in non-pregnant women (P=0.0001). Furthermore, no significant differences were observed in Breg cell percentages between normal pregnant and non-pregnant women. Evaluation of IL-10 concentration in the serum of women who had participated in this study showed no significant differences between the three groups.
ConclusionBased on our results, the number of Breg cells was significantly lower in RPL women than in healthy non-pregnant and normal-pregnant women, which shows the significance of these cells in the maintenance of normal pregnancy. However, we could not detect significant differences in the serum levels of IL-10, bringing to mind the notion that the beneficial and supportive function of these cells during pregnancy might be independent of IL-10 secretion. by these cells. Thus, screening of Breg cells in women with pregnancy complications, especially RPL, could be helpful for predicting a healthy pregnancy.
Keywords: Regulatory B Cell, Reproductive Immunology, IL-10, RPL} -
Background
Cytokines are important in many pathobiological processes of chronic obstructive pulmonary disease (COPD). This study aimed to determine the relationship between serum levels of interleukin-33 (IL- 33) and the severity of COPD disease.
MethodIn this cross-sectional research, the study population consisted of all COPD patients referring to the pulmonary clinic of Imam-Ali Hospital of Zahedan city. Sixty patients were selected using the available sampling method. Serum IL-33 levels were measured by the quantitative ELISA method.
ResultsOf 60 patients, 23 (38.3%) and 37 (61.7%) subjects were male and female, respectively. Analysis shows a significant difference between serum IL-33 of the two groups with regard to the severity of COPD disease. There was a statistically significant negative relationship between the serum level of IL-33 and the severity (decrease of forced expiratory volume in one second (FEV1)) of COPD disease.
ConclusionOur results indicate a systemic release of IL-33 correlated with the severity of COPD.
Keywords: Cytokine, IL-33, Severity, Chronic Obstructive Pulmonary Disease, COPD} -
BackgroundAcute lung injury is respiratory failure due to various causes. Increased inflammatory and oxidative processes are recognized to play an essential role in the etiology of ARDS. Abelmoschus esculentus is an herbal product used to treat various diseases due to its anti-inflammatory and antioxidant effects. We aimed to investigate whether Abelmoschus esculentus has an effect on acute lung injury.Materials and MethodsIn this experimental study, we used the ethanol extract of Abelmoschus esculentus seed. It divided forty male Wistar rats into five equal groups: 1) control, 2) Abelmoschus esculentus, 3) lipopolysaccharide,
4) lipopolysaccharide+Abelmoschus esculentus, and 5) lipopolysaccharide+ Abelmoschus esculentus +dexamethasone groups.ResultsIn the lipopolysaccharide group, native thiol, total thiol, IL-10, and IFN-ɣ levels significantly changed. Abelmoschus esculentus was effective when used with dexamethasone in increasing native thiol and total thiol values (p=0.008 and p=0.004, respectively). On the other hand, when Abelmoschus esculentus was used alone, it significantly increased IL-10 levels and decreased IFN-ɣ levels in the lipopolysaccharide group (p=0.025 and p<0.001, respectively). Additionally, improvements were noted in histological findings of alveolar congestion (p=0.006), intra-alveolar hemorrhage (p=0.006), and intra-alveolar macrophages (p=0.001).ConclusionAbelmoschus esculentus, with its anti-inflammatory effect, may represent a new potential for treating acute lung injury.Keywords: Abelmoschus Esculentus, Acute Lung Injury, IL-10, IFN-Ɣ, Thiol} -
پیش زمینه و هدف
AKT، PI3K و IL-1 با دیابت و التهاب قلبی مرتبط هستند. هدف از مطالعه حاضر، بررسی مارکرهای التهاب قلبی به واسطه بیان ژن Akt، PI3K قلبی و فاکتور IL-1β سرمی با تمرین ترکیبی و مصرف مکمل تورین در رت های نر ویستار دیابتی شده بود.
مواد و روش کاردر این مطالعه تجربی، 30 رت نر ویستار بالغ با دامنه وزنی 300-250 گرم و سن متوسط 6 هفته، به صورت تصادفی در 3 گروه شامل: گروه تمرین-مکمل دیابتی (T+S) (10 رت)، گروه کنترل دیابتی (CD) (10 رت)، و گروه کنترل سالم (CH) (10 رت) قرار گرفتند. برای دیابتی کردن رت ها، 55 میلی گرم بر هر کیلوگرم از وزن بدن استرپتوزوسین تزریق شد. قند خون بالای 250 میلی گرم بر دسی لیتر در خون به عنوان دیابتی در نظر گرفته شد. تمرینات ترکیبی و مصرف مکمل تورین به مدت هشت هفته و 5 بار در هفته انجام شد. مکمل تورین به صورت محلول 1درصد در آب روزانه مصرف شد. برای مقایسه تغییرات بین گروهی و درون گروهی متغیرها، ANOVA دوراهه و تعقیبی توکی استفاده شد. سطح معناداری 05/0 بود.
یافته هابیان ژن PI3K در گروه تمرین - مکمل دیابتی در مقایسه با گروه کنترل دیابتی افزایش معنادار داشت (001/0 P=). میزان سرمی فاکتور IL-1β در گروه تمرین - مکمل دیابتی در مقایسه با گروه کنترل دیابتی کاهش معنادار داشت (001/0 P=). بااین حال، بیان ژن AKT در گروه تمرین - مکمل دیابتی در مقایسه با کنترل دیابتی تغییر معناداری نداشت.
بحث و نتیجه گیریبه نظر می رسد تمرینات ترکیبی به همراه مصرف مکمل تورین می تواند باعث افزایش PI3K، کاهش IL-1β و ایجاد تغییرات مطلوب در متابولیسم گلوکز شده و عوامل التهاب قلبی در افراد دیابتی را بهبود بخشد. بنابراین پیشنهاد می شود از تمرینات ترکیبی و مصرف مکمل تورین به عنوان یک روش غیرتهاجمی و کم هزینه در کاهش و بهبود مارکرهای التهابی قلبی افراد دیابتی استفاده شود.
کلید واژگان: AKT, تمرین ترکیبی, دیابت, IL-1Β, PI3K, مکمل تورین}Background & AimsAKT, PI3K and IL-1 are related to diabetes and cardiac inflammatory. The aim of this study was to investigating cardiac inflammatory markers via AKT, cardiac PI3K gene expression and serum IL-1β with combined exercise and consumption of taurine supplementation in diabetic male Wistar rats.
Material & Methodsin this experimental study, 30 male adult Wistar rats weighing 250-300 grams and 6 weeks old were randomly divided into 3 groups including: diabetic exercise+supplement group (T+S) (10 rats), diabetic control group (CD) (10 rats), and healthy control group (CH) (10 rats). To make rats diabetic, 55 mg/kg body weight of streptozocin was injected. Blood sugar above 250 mg/dL was considered as diabetes. Combined exercises and taurine supplementation were performed for eight weeks and 5 times a week. Taurine supplement was taken as a 1% solution in water daily. To compare the inter-group and intra-group changes of the variables, two-way ANOVA and Tukey's follow-up were used. The significance level was 0.05.
ResultsPI3K gene expression was significantly increased in the diabetic exercise+supplement group compared to the diabetic control group (P=0.001). The serum level of IL-1β factor in the diabetic exercise-supplement group had a significant decrease in comparison with the diabetic control group (P=0.001). However, there was no significant change in AKT gene expression in the diabetic exercise+supplement group compared to the diabetic control group.
ConclusionIt seems that combined exercises with consumption of taurine supplementation may lead to the improvement of heart inflammatory factors in diabetic people while increasing PI3K, decreasing IL-1β and creating favorable changes in glucose metabolism. Therefore, it is suggested to use combined exercises and consumption of taurine as a non-invasive and low-cost method in improving cardiac inflammatory markers in diabetic persons.
Keywords: AKT, Combined Exercise, Diabetes, IL-1Β, PI3K, Taurine Supplement} -
Purpose
Rheumatoid arthritis (RA) is a type of autoimmune disease that results in chronic inflammation of the joint synovial tissue, leading to joint damage and significant disability. Despite ongoing research, the exact cause of RA remains unclear, and current treatments have limitations. This study explores the potential of utilizing interleukin-1 receptor antagonist (IL-1RA) and anti-inflammatory macrophages polarized in the vicinity of the supernatant from allogeneic mesenchymal stem cells (MSCs) as a novel therapeutic approach for RA.
MethodsAn expression cassette containing the IL-1RA gene was constructed and expressed in E. coli BL21. The resulting protein was purified and stabilized for use in in vivo experiments. Bone marrow MSCs were isolated and used to produce anti-inflammatory M2 macrophages from the isolated peripheral blood monocytes. The macrophages were then used to treat mice with RA induced by collagen type II.
ResultsThe combination of IL-1RA and M2 macrophages improved clinical and histopathological symptoms of the disease, reduced levels of inflammatory factors, and modulated the immune system in the treated mouse groups. The results showed that this combinatory therapy had a synergistic effect for RA treatment.
ConclusionThe simultaneous use of IL-1RA and M2 cells could be a promising approach for the treatment of RA. This combinatory therapy has the potential to improve the disease and decrease the severity of inflammation in patients with RA.
Keywords: Rheumatoid Arthritis, IL-1RA, Anti-Inflammatory Macrophages, Synergistic Effect, Novel Therapeutic Approach, Combinatory Therapy} -
Background
There is evident inter-individual variability in women's responses to Chlamydial infections and reproductive tract problems. Women's genetic variations within the Interleukin-10 (IL-10) gene have been linked to variances in response to Chlamydia trachomatis infection. This study was aimed to demonstrate the profound association of IL-10 with infertility and demonstrate the role of IL-10 (-592 C/A rs1800872) and (-1082 A>G rs1800896) single nucleotide polymorphism (SNPs) gene in the susceptibility and severity of a C. trachomatis infection.
MethodIn this evaluation study, serum IL-10 concentration was measured in 134 women diagnosed with infertility and 50 healthy volunteers by enzyme-linked immunosorbent assay (ELISA). The tetra-amplification refractory mutation system-PCR (T-ARMS-PCR) analysis was performed to detect the genotyping of the rs1800872 and rs1800896 SNPs genes.
ResultBoth female groups were positive for anti-chlamydial IgM antibody, but the intensity of response differed between cases. At the same time, the incidence of genital C. trachomatis by PCR was 46.2% in infertile women. The serum concentration of IL10 was lower in infertile women than healthy participants and higher in infertile C. trachomatis-positive women compared to infertile C. trachomatis-negative in all groups except endometriosis (Endo) infertility. In rs1800872, the CA genotype and C allele are associated with an increased risk for infertility, except in polycystic ovarian syndrome (PCOS), which is an A allele. In the case of rs1800896, the AG genotype and G allele show a greater risk for infertility.
ConclusionsOur results confirmed that rs1800872 and rs1800896 gene polymorphisms were associated with an increased risk of C. trachomatis infection.
Keywords: Chlamydia Trachomatis, Female Infertility, IL-10, SNP} -
Background
The growing threat of antibiotic resistance and Klebsiella pneumoniae infection in healthcare settings highlights the urgent need for innovative solutions, such as vaccines, to address these challenges. This study sought to assess the potential of using K. pneumoniae OmpA as a vaccine candidate through both in silico and in vivo analyses.
MethodsThe study examined the OmpA protein sequence for subcellular localization, antigenicity, allergenicity, similarity to the human proteome, physicochemical properties, B-cell epitopes, MHC binding sites, tertiary structure predictions, molecular docking, and immune response simulations. The ompA gene was cloned into the pET-28a (+) vector, expressed, purified and confirmed using Western blotting analysis. IgG levels in the serum of the immunized mice were measured using ELISA with dilutions ranging from 1:100 to 1:6400, targeting rOmpA and K. pneumoniae ATCC 13883. The sensitivity and specificity of the ELISA method were also assessed.
ResultsThe bioinformatics analysis identified rOmpA as a promising vaccine candidate. The immunized group demonstrated significant production of specific total IgG antibodies against rOmpA and K. pneumoniae ATCC1 13883, as compared to the control group (p < 0.0001). The titers of antibodies produced in response to bacterial exposure did not show any significant difference when compared to the anti-rOmpA antibodies (p > 0.05). The ELISA test sensitivity was 1:3200, and the antibodies in the serum could accurately recognize K. pneumoniae cells.
ConclusionThis study is a significant advancement in the development of a potential vaccine against K. pneumoniae that relies on OmpA. Nevertheless, additional experimental analyses are required.
Keywords: Glial Fibrillary Acidic Protein, IL-1Β, Post-Traumatic Epilepsy, Traumatic Brain Injury} -
Background and purpose
Anakinra must be injected daily due to its short half-life and this leads to lower patient compliance. Therefore, the aim of this study was to produce an interleukin-1 receptor antagonist (IL-1Ra) with albumin binding domain (ABD) as a novel fusion protein and evaluate its binding ability to albumin and its biological effects.
Experimental approach:
The three-dimensional structure of IL-1Ra-ABD was predicted by MODELLER software and its interaction with IL-1R was evaluated by the HADDOCK server. The expression of IL-1Ra-ABD was performed in E. coli in fusion with intein 1 of pTWIN1 in soluble form and then purified. The affinity of IL-1Ra-ABD to human serum albumin (HSA) was determined on native-PAGE, and its release percent toward time was evaluated. Moreover, an MTT assay was used to determine the antagonizing properties of recombinant IL-1Ra-ABD against IL-1β in A375 and HEK293 cell lines.
Findings/ ResultsThe stable complex of IL-1Ra-ABD with IL-1R established the absence of steric hindrance due to the addition of ABD to IL-1Ra. The expression induction of intein 1-IL-1Ra-ABD using 0.1 mM IPTG at 15 °C, and its cleavage represented bands approximately in 50 and 23 kDa. Furthermore, about 78% of IL-1Ra-ABD was attached to the HSA after 2 h of incubation, and the MTT assay showed no significant differences between the effects of IL-1Ra-ABD and native IL-1Ra in cell survival.
Conclusions and implications:
The production of soluble IL-1Ra-ABD with no significant differences in IL-1Ra antagonizing effects was successfully performed. IL-1Ra-ABD showed suitable interaction with HSA and was released over time. However, the half-life of IL-1Ra-ABD in vivo must be determined in the subsequent investigations.
Keywords: Albumin Binding Domain, Human Serum Albumin, IL-1Ra, Intein} -
Background & Objective
Besides the clinical and laboratory research on the COVID-19 virus, the bioinformatics study in the field of genetics of immunity to COVID-19 is of particular importance. In this account, studies show that in patients with COVID-19, the level of tumor necrosis alpha (TNFα) and interleukin-6 (IL-6) is high and in severe cases of COVID-19, the production of IL-6, TNF-α, and other cytokines increases profoundly. On the other hand, investigating the molecular structure and receptors of IL-6 and TNFα and the structural analysis of the receptor proteins may potentially help to develop new therapeutic plans for COVID-19 infection.
MethodsTo identify genes with significant and different expressions in patients with COVID-19 in a microarray data set containing transcriptional profiles from GEO as a functional genomic database the GEO query package version 2.64.2 in a programming language R version 4.2.1 was downloaded. In this way, functional enrichment analysis for DEGs, WikiPathways, REGO, gene ontology, and STRING database was also investigated and employed.
ResultsThe structure and function of pro-inflammatory cytokines TNFα and IL-6 involved in the pathogenesis of COVID-19 were investigated, and in general, after performing various analyses in this study and extracting A series of genes with different expressions from the KEGG database, the final 5 DEGs include CXCL14, CXCL6, CCL8, CXCR1, TNFRSF10, and the relationship and expression effects of them were observed in different pathways.
ConclusionIL-6 and TNFα were involved in immunological processes that had a direct and indirect relationship with the activation of cytokines, including IL6 and TNF-a, and cytokine storm, and this indicates their role in the formation of problems and complications, including ARDS, in COVID-19 patients. Of course, determining the effectiveness of each of these genes requires more specialized and clinical studies.
Keywords: Bioinformatics, COVID-19, Cytokine, IL-6, TNF-A} -
This investigation aimed to assess the influence of vitamin D3 and calcium on certain immunological and biochemical factors in rats. Forty-eight male rats were assigned to eight distinct groups. There were two main groups. The first group had standard Diet-Fed rats (Vit. D3, Ca+2, Vit. D3, and Ca+2, Sunlight, and Fasting). The second group had high-fat diet-fed rats (HFD and HFD with Vit. D3 and Ca+2), also compared to the control group. The administration of calcium and vitamin D supplements lasted for six weeks. The levels of vitamin K, IL-10, TNF-α, IgM, and Osteocalcin were determined by applying ELISA. The administration of Vitamin D and calcium has been observed to significantly increase Vitamin D, Vitamin K, and Osteocalcin levels in the rats fed on the typical diet. In contrast, sunlight exposure and fasting for the same duration did not substantially impact serum vitamin D and Osteocalcin in rats fed a normal diet. Additionally, a significant reduction in the concentration of Vitamin K in the serum was detected in the experimental rats fed on a normal diet and subjected to sunlight and fasting. The administration of HFD for six weeks was found to provoke hyperglycemia in experimental rats. However, it did not elicit any significant influence on the concentration of vitamin D, vitamin K, and osteocalcin. Furthermore, using calcium and vitamin D for six weeks negatively impacted immune disturbances in rats consuming a normal diet (ND) or HFD by regulating anti-inflammatory cytokine (IL-10) secretion.
Keywords: TNF-α, ND, HFD, IL-10, Vitamin D, Vitamin K, Osteocalcin} -
Today, camel milk consumption in the Middle East is trendy because it is believed that it reduces the risk of cancer. Recently, studies have discovered that most of milk's beneficial effects are because of its nanoparticles, especially exosomes. The objective of the present research was to investigate the anti-cancer effects of camel milk exosomes (CMEXOs) in the murine colorectal cancer cell line (CT-26). Our findings verified the existence of exosomes measuring approximately 114.1±3.4 nm in diameter. Through MTT and migration assays, we established that CMEXOs exhibit dose-dependent anti-proliferative and anti-migration effects on the CT-26 cell line. Furthermore, our study showed that treatment with CMEXOs led to a reduction in TNF-α and IL-6 gene expression in CT-26 cells. While additional in vivo studies are required, our data demonstrate that CMEXOs have anti-proliferative and anti-migration effects on CT-26, possibly by influencing crucial genes within the inflammation pathway.
Keywords: Camel Milk, Colorectal Cancer, Exosomes, IL-6, TNF-Α} -
International Journal of Reproductive BioMedicine، سال بیست و دوم شماره 3 (پیاپی 170، Mar 2024)، صص 235 -244مقدمه
آندومتریوز یک اختلال التهابی مزمن مرتبط با استروژن است که با تکثیر سلول های آندومتر در مکانی خارج از رحم شناخته می شود. حضور زیاد سلول های ایمنی در مایع صفاقی زنان مبتلا به اندومتریوز، دخالت سیستم ایمنی در پاتوژنز بیماری را تایید می کند. سلول های MAIT با تولید IL-17، IFN-γ و TNF-α، تاثیر غیرقابل انکاری بر ایمنی مخاط ایفا می کنند. عملکرد این سلول ها در پاتوژنز اندومتریوز کمتر مورد بررسی قرار گرفته است.
هدفهدف از این مطالعه بررسی نفوذ سلول MAIT با استفاده از تعیین سطح بیان ژن های Va7.2-Jα33 در بافت یوتوپیک و اکتوپیک ضایعات آندومتریوز است.
مواد و روش هادر مطالعه مورد-شاهدی، نمونه های مورد آزمایش شامل 20 بافت اوتوپیک و 20 بافت نابجا زنان مبتلا به اندومتریوز و 20 بافت آندومتر رحم زنان در گروه شاهد بود. بیان ژن های TNF-α، IL-17A، IFN-γ توسط (q-RT-PCR) مورد تجزیه و تحلیل قرار گرفت.
نتایجبر اساس نتایج مطالعه، بیان ژن های Va7.2-Jα33 در رحم و بافت های یوتوپیک آندومتر در مقایسه با کنترل داخلی ژن و همچنین در بافت های نابجا، افزایش قابل توجهی را نشان نداد. تجزیه و تحلیل همبستگی یک رابطه مثبت بین ژن های بیان Vα7.2-Jα33 و سطوح IFN-γ در بافت های نابجا تایید کرد.
نتیجه گیریبا توجه به بیان کم ژن اختصاصی سلول های MAIT در بافت نابجا، می توان نتیجه گرفت که این سلول ها به میزان کمی در محیط اندومتریوز حضور دارند و با ترشح IFN-γ احتمال نقش آنها در پیشرفت اندومتریوز وجود دارد.
کلید واژگان: آندومتریوزیس, MAIT, IFN-Γ, TNF-Α, TCR V Alpha 7.2 J Alpha33, IL-17}BackgroundEndometriosis is a chronic estrogen-related inflammatory disorder that is known by proliferating endometrial cells in a place outside the uterus. The high presence of immune cells in the peritoneal fluid of women with endometriosis confirms the involvement of the immune system in the pathogenesis of the disease. Mucosal-associated invariant T (MAIT) cells play an undeniable impact on mucosal immunity by the production of interleukin-17, interferon-gamma (IFN-γ), and tumor necrosis factor-alpha. The function of the cells in the pathogenesis of endometriosis is less investigated.
ObjectiveThis study aims to investigate the infiltration of MAIT cells by using the determination levels of Vα7.2-Jα33 gene expression in eutopic and ectopic tissue of endometriosis lesions.
Materials and MethodsIn this case-control study, the tested samples include 20 eutopic and 20 ectopic tissues of women with endometriosis and 20 uterine endometrial tissues of women in the control group. Expressions of the Vα7.2-Jα33 tumor necrosis factor-alpha, interleukin-17A, and IFN-γ genes were analyzed by quantitative reverse transcriptase-polymerase chain reaction.
ResultsAccording to the results, Vα7.2-Jα33 gene expression did not show substantial elevation in the uterine and eutopic endometrial tissues compared to internal gene control as well as in ectopic tissues. Correlation analysis approved a positive relationship between Vα7.2-Jα33 expression genes and IFN-γ levels in ectopic tissues.
ConclusionConsidering the low-expression specific gene of MAIT cells in ectopic tissue, it can be concluded that these cells are present in the endometriotic environment to a certain extent, and there is a possibility of their role in the progression of endometriosis by secreting IFN-γ.
Keywords: Endometriosis, MAIT, IFN-Γ, TNF-Α, TCR V Alpha 7.2-J Alpha33, IL-17} -
International Journal of Hematology-Oncology and Stem Cell Research, Volume:18 Issue: 2, Apr 2024, PP 140 -146Background
Chronic lymphocytic leukemia (CLL) is the most common leukemia in adults. Currently, several biomarkers are being used as CLL prognosticators, including elevated protein levels, elevated RNA levels, gene mutations, and epigenetic changes.
Materials and MethodsThis study is a prospective study conducted on 55 patients newly diagnosed with CLL, serum IL-6 level was measured initially and after a 6-month treatment course. Correlation with the course of the disease and the known CLL prognostic parameters was done initially and after 6 months.
ResultsThe initial serum IL-6 level in the patient group (pre-treatment) ranges from 36-91 pg/mL (median 57), and in the patient group (post-treatment) ranges from 1-32 pg/mL (median 2). Serum IL-6 level was positively correlated with WBC count, β2 microglobulin, LDH, ESR, B symptoms, Uric Acid, BM Aspirate (% of lymphocytes), and Binet and Rai staging systems.
ConclusionSerum IL-6 is a useful poor prognostic marker in newly diagnosed CLL patients; its prognostic value goes with the other known prognostic markers such as the BM lymphocyte count, ESR, and LDH.
Keywords: Chronic lymphocytic leukemia (CLL), IL-6, Prognostic markers, Cytogenetics} -
Psoriasis is an autoimmune disease characterized by keratinocyte hyperproliferation and skin thickening. Psoriasis is caused by a complicated interaction between the innate and acquired immune systems. In the skin, this reaction produces abnormal T helper cell (Th1, Th17, and Th23) reactivation. Keratinocyte hyperproliferation is caused by increased cell signaling via cytokines interleukin-17A (IL-17A), IL-17, IL-23, tumor necrosis factor alpha (TNF-α), and interferon-gamma (INF-γ). Obesity, free fatty acids, microorganisms in the skin and digestive tract, free radicals in the body, and the cardiovascular system are also essential variables in psoriasis. Several variables influence the cytokine activation of the IL17/IL-23 pathway. Obesity, which is marked by changes in lipid profile in psoriasis patients, is linked to increased oxidative stress and the generation of proinflammatory cytokines, both of which can potentially trigger psoriasis relapse. Antioxidant-rich diet and intake can be employed as one of the stages in preventing psoriasis recurrence.
Keywords: Psoriasis, Autoimmune, Obese, Oxidative stress, IL-17, IL-23 pathway} -
Background
Melatonin, the controlling hormone of the sleep–wake cycle, has acquired attention due to its role in immunomodulation, anti-inflammation, as well as its proapoptotic effects. Wnt/β-catenin signaling can modulate cancer progression by promoting cell division and migration, while miR-let-7b may inhibit cell growth, migration, and invasion by affecting the function of adaptive immune cells. This work was designed to detect the effect of using melatonin as an immunomodulating therapeutic approach to control the progression of chemically induced hepatocellular carcinoma (HCC).
MethodsThirty male rats were equally divided into control, HCC, and melatonin-HCC groups. Animals in the HCC and melatonin-HCC groups were injected with diethylnitrosamine (intraperitoneal single dose) followed by repeated carbon-tetrachloride subcutaneous injection once weekly for six weeks. Melatonin was given from the first week of the study and continued during the process of HCC induction.
ResultsIn the HCC group, the levels of tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), and Wnt/β-catenin expression significantly increased, while there was a downregulation of microRNA Let7b. Melatonin administration reversed these changes, along with an increase in hepatic content of interleukin-2 (IL-2) and caspase-3.
ConclusionsMelatonin exerted hepatic immunomodulating changes, in addition to proapoptotic and antiangiogenic effects, illustrated by increased IL-2, caspase-3, and decreased VEGF levels, respectively. Moreover, the use of melatonin during hepatocarcinogenesis positively modulated the disrupted expression of microRNA let7b and Wnt/β-catenin significantly.
Keywords: Hepatocellular carcinoma, Melatonin, IL-2, miRNA Let7b, Wnt pathway, β-catenin} -
مقدمه و هدفبیماری پارکینسون یک بیماری پیش رونده سیستم عصبی است که علاوه بر سیستم عصبی مرکزی، دیگر اعضای بدن را نیز تحت تاثیر قرار می دهد. مطالعه حاضر با هدف بررسی اثر یک دوره تمرین تناوبی شنا با شدت بالا بر بیان ژن هیپوکامپی برخی عوامل التهابی در موش های صحرایی مبتلا به پارکینسون انجام شد.مواد و روش هادر مطالعه تجربی حاضر، 21 سر موش نر صحرایی نژاد ویستار 10 -8 هفته ای با میانگین وزن 2/10 ± 200 گرم به طور تصادفی در سه گروه کنترل سالم، بیمار و تمرین شنا قرار گرفتند. القای بیماری به واسطه تزریق 1 میلی گرم به ازای هر کیلو وزن بدن رزرپین و طی 5 روز صورت گرفت. گروه تمرین به مدت شش هفته 20 نوبت 30 ثانیه ای شنا با 30 ثانیه استراحت بین هر نوبت انجام دادند. نتایج با استفاده از تحلیل واریانس یک راهه و آزمون تعقیبی LSD در سطح معنی داری 05/0>P مورد تجزیه و تحلیل قرار گرفتند.نتایجنتایج نشان داد بیان ژن هیپوکامپی TNF-α پس از اجرای تمرینات تناوبی شنا در گروه تمرین نسبت به گروه بیمار (01/0=p) به طور معنی داری کاهش داشته است درحالی که علی رغم پایین تربودن بیان ژن IL-1β در گروه تمرین نسبت به گروه بیمار، اما این کاهش معنی دار نبود (6/0=p).نتیجه گیریبا توجه به نتایج به دست آمده اجرای تمرینات تناوبی شنا با شدت بالا در کاهش بیان ژن برخی عوامل التهابی در بافت هیپوکامپ موش های مبتلا به پارکینسون موثر است و ممکن است به این واسطه به بهبود روند بیماری کمک کند.کلید واژگان: تمرین تناوبی شنا, بیماری پارکینسون, TNF-α, IL-1β}Background and ObjectiveParkinson disease (PD) is a progressive neurodegenerative disease that affects peripheral organs as well as the central nervous system (CNS). The present study was done to examine the effect of high intensity swimming interval training on gene expression of some hippocampal inflammatory markers in rats with PD.Materials and MethodsIn this experimental study, 21 male Wistar rats (aged 8-10 weeks; weight 200 ± 10.2 grams) were divided into three groups including: healthy control, PD and training. PD was induced by injection of 1 mg/kg of reserpine during 5 days. The rats in the training group performed 6 weeks of HIIT including 20 times of 30 seconds of swimming with 30 seconds of rest between each time. Data were analyzed using one-way ANOVA and LSD post hoc test were run using SPSS-22 at the P <0.05.ResultsData revealed that TNF-α gene expression was reduced after 6 weeks of swimming interval training (p=0.01) compared to the PD group. We observed non-significant decrease in IL-1β after the intervention as compared to the PD group (p=0.6).ConclusionAccording the results of this study, high intensity swimming interval training is effective in reducing the gene expression of some hippocampal inflammatory markers in rats with PD and it is suggested as the effective strategy to improve PD.Keywords: Swimming interval training, Parkinson’s disease, TNF-α, IL-1β}
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BackgroundUnderstanding the effects of epigenetic factors on the pathogenesis of rheumatoid arthritis (RA) is important for the early diagnosis and therapeutic intervention of this disease. MicroRNA-150 (miR-150) exerts an important influence on the development and function of lymphocytes. However, the role of miR-150 in the pathogenesis of RA remains unclear.ObjectiveTo explore the role of miR-150 in the pathogenesis of RA and the related immune mechanism.MethodsIn this study, we used miR-150 knock-out (miR-150KO) and created animal models of RA. Flow cytometry, immunohistochemistry, and real-time RT-PCR were employed to assess the frequency of T cell subsets and cytokines expression.ResultsCompared to wild-type (WT) mice, the onset of RA was postponed and the incidence of RA was reduced in miR-150KO mice. The expression of IL-4 and IFN-γ significantly increased while the expression of IL-17 decreased significantly in NKT and CD4+ T cells of KO mice compared to that of WT mice after RA induction. In addition, the expression of IL-4 and IFN-γ increased while the expression of IL-17 decreased significantly in the joint tissues of KO mice compared to that of WT mice. Furthermore, the mRNA expression of TNF-α and IL-17 decreased significantly in the synovial fluid cells of KO mice compared to that of the WT mice after RA induction.ConclusionMiR-150 deficiency decreases the expression of IL-17 in T cells and joint tissues, and alleviates the occurrence and progression of RA in mice.Keywords: IL-17, MicroRNA-150, Rheumatoid Arthritis}
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Background
Traumatic brain injury or TBI can underlie epilepsy. Prevention of PTE has been of great interest to scientists. Given the antiepileptic, antioxidant and anti-inflammatory activities of curcumin, we examined whether this compound can affect epileptogenesis in rats after TBI.
MethodsCurcumin was injected once a day for two weeks. TBI was induced in the temporal cortex of anesthetized rats using a CCI device. One day after TBI, PTZ, 35 mg/kg, was injected i.p. every other day until manifestation of generalized seizures. The number of PTZ injections was then recorded. Moreover, the extent of cortical and hippocampal IL-1β and GFAP expression in the epileptic rats were measured by Western blot analysis.
ResultsCurcumin 50 and 150 mg/kg prevented the development of kindling, wherase TBI accelerated the rate of kindling. Curcumin 20 mg/kg prohibited kindling facilitation by TBI, and reduced the expression of IL-1β and GFAP induced by TBI.
ConclusionCurcumin can stop the acceleration of epileptogenesis after TBI in rats. Inhibiting hippocampal and cortical overexpression of IL-1β and GFAP seems to be involved in this activity.
Keywords: Glial fibrillary acidic protein, IL-1β, Post-traumatic epilepsy, Traumatic brain injury} -
BackgroundPulmonary neutrophils may play a crucial role in the development of bronchiolitis obliterans (BO) following measles virus infection. IL-27 could potentially have a negative regulatory effect on the release of reactive oxygen species and cytotoxic granules in neutrophils.ObjectiveTo investigate the levels of IL-27 in the bronchoalveolar lavage fluid (BALF) of children with post-infectious bronchiolitis obliterans (PIBO) and analyze the relationship between IL-27 levels and neutrophil proportions.MethodsA total of 24 children with PIBO were recruited for the experimental group, while 23 children with bronchial foreign bodies were included in the control group. Bronchoscopic alveolar lavage was performed in both groups. The levels of IL-27 in BALF were measured using enzyme-linked immunosorbent assay (ELISA). The proportions of neutrophils in BALF were determined by smear staining. The relationship between the levels of IL-27 in BALF and the neutrophil proportions was analyzed by the Pearson test.ResultsThe levels of IL-27 in BALF were significantly lower in children with PIBO compared to children with bronchial foreign bodies (p<0.05). Additionally, the proportions of neutrophils in BALF were significantly higher in children with PIBO compared to children with bronchial foreign bodies (p<0.05). The levels of IL-27 were negatively correlated with the neutrophil proportions in BALF in children with PIBO (p<0.05), but not in children with bronchial foreign bodies (p>0.05).ConclusionThe present study suggests that a decrease in IL-27 may be associated with an increase in neutrophils in BALF and may contribute to the pathogenesis of PIBO.Keywords: Bronchoalveolar Lavage Fluid, children, IL-27, Post-Infectious Bronchiolitis Obliterans}
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زمینه و هدف
سنجش میزان و تولید سایتوکاین ها به ویژه اینترلوکین های 10 و 12 ابزار مهمی در بررسی پاسخ های ایمنی در برابر محرک هایی نظیر عوامل بیماری زا از جمله هلیکوباکتر پیلوری می باشد. هدف از این مطالعه بررسی میزان اینترلوکین های 10 و 12 در بیماران مبتال به هلیکوباکترپیلوری و مقایسه آن با افراد سالم به منظور یافتن ارتباط مقادیر آنها با بروز بیماری و گسترش آن می باشد.
روش بررسینمونه سرم 66 فرد مبتال از نظر سرولوژی IgM ضد هلیکوباکترپیلوری، توسط روش االیزا مورد بررسی قرار گرفت و در مجموع، 61 بیمار حایز شرایط مورد سنجش غلظت سرمی اینترلوکین 10 و 12 بوسیله کیت اختصاصی االیزا سنجش سرمی شدند. در این مطالعه از آزمون های test-T و تحلیل واریانس دوطرفه (ANOVA way-Two) با استفاده از نرم افزار آماری SPSS ورژن 21 تجزیه و تحلیل آماری شدند.
نتایجنتایج این مطالعه نشان داد که سطح 12-IL و 10-IL در سرم بیماران مبتال به هلیکوباکتر پ یلوری بیشتر از افراد سالم است. و این احتماال نشان دهنده نقش محافظتی این سیتوکین ها در بیماران است.
نتیجه گیریبا توجه به نتایج بدست آمده می توان گفت در افراد مبتال به هلیکوباکتر پیلوری، با افزایش میزان سطح سرمی اینترلوکین 10 و 12عملکرد بیولوژیکی آنها، موجب پیشرفت بیماری شده و موجب اختالل در سیستم ایمنی بدن می گردد.
کلید واژگان: هلیکوباکتر پیلوری, اینترلوکین 10و 12, سرولوژی, االیز}Background and AimMeasuring the amount and production of cytokines, especially IL-10 and IL-12, is an important tool in investigating immune responses against stimuli such as pathogens, including Helicobacter pylori. This study aims to investigate the level of interleukins 10 and 12 in patients with Helicobacter pylori and compare it with healthy people to find the relationship between their levels and the incidence and spread of the disease.
Materials and methodsThe serum samples of 66 patients were analyzed for IgM serology against Helicobacter pylori by ELISA method. In total, 61 patients with conditions were measured serum concentration of IL-10 and IL-12 in specific ELISA kits. In this study, T-test and two- way ANOVA tests were statistically analyzed using SPSS (version 21) statistical software.
ResultsThe results of this study showed that the levels of IL-12 and IL-10 in the serum of patients with H. pylori is more than that of healthy individuals. And this probably indicates the protective effect of these cytokines in patients.
ConclusionAccording to the obtained results, it can be said that in people suffering from Helicobacter pylori, by increasing the serum level of IL-10 and IL-12, their biological function leads to the progress of the disease and causes disorders in the body's immune system.
Keywords: Helicobacter pylori, IL-10, IL-12, Serology, ELISA}
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