جستجوی مقالات مرتبط با کلیدواژه « interleukin-17 » در نشریات گروه « پزشکی »

Objective

Multiple sclerosis (MS) has a multi-factorial etiology involving genetic factors. Fingolimod (Gilenya ®, FTY720) modulates the G-protein-coupled sphingosine 1-phosphate (S1P) receptors, S1PR1, 2, 3, 4 and 5. Variation in the human S1PR1 coding sequence results in heterogeneity in the function of the receptor. Interleukin-17, producing CD4+ T cells, tends to be increased after treatment with Fingolimod. The aim of the study was to investigate single-nucleotide polymorphisms (SNPs) in the S1PR1 gene or interleukin-17 (IL-17) levels in a small group of Iranian relapsing-remitting MS patients treated with Fingolimod.

In this case-control study, the genomic DNA of 94 MS patients treated with Fingolimod was extracted and Sanger sequencing was performed on polymerase chain reaction (PCR) products to detect variants in the S1PR1 gene. Quantification of IL-17 from the serum of the patients was performed using a commercially available enzyme-linked immunosorbent assay (ELISA).

Among 94 relapsing-remitting MS patients treated with Fingolimod, 69 (73.4%) were responders and 25 (26.6%) were non-responders. There were four novel and five common SNPs in the S1PR1 gene and no significant association between SNP genotype and drug response was detected. In a subset of 34 patients, there was no significant difference in IL-17 serum concentrations before or after treatment and no association with S1PR1 polymorphisms was determined.

This study is the first in Iran to investigate association between SNPs of the S1PR1 gene or IL-17 levels with fingolimod response in a small group of Iranian relapsing remitting MS patients. There was no association with S1PR1 gene SNPs or IL-17 levels before or after treatment.

Interest in probiotic use for respiratory allergies has increased. In this regard, the present study aimed to evaluate the effect of cell wall extractof Saccharomyces boulardii on Aspergillus fumigatus as an allergenic fungus and itseffectiveness in reducing inflammatory cytokines in A549 cells sensitized with A. fumigatus conidia.

Cell wall of S. boulardii was prepared and challenged by A.fumigatus conidia at various concentrations.Secretory protease activity was tested usingthe Casein method. The A. fumigatus allergen 1 (Asp f1) gene expression was calculated by quantitative real-time polymerase chain reaction (qRT-PCR). In another experiment,qRT-PCR was used to examine gene expression of interleukin 13 and interleukin 17 by A549 lung epithelial cells exposed to A. fumigatus conidia and treated with different concentrations of S. boulardii cell wall extract.

Saccharomyces boulardii cell wall extract significantly reduced the proteaseactivity of A. fumigatus at concentrations of 10 and 20 mg/ml (P<0.05). The Asp f1 gene expression was significantly down-regulated in each concentration of S. boulardii cell wallextract (P<0.05). Aspergillus fumigatus conidia upregulated the expression of IL-13 and IL-17 in A549 cells, and S. boulardii cell wall extract could downregulate the expression of the mentioned cytokines at concentrations of 10 and 20 mg/ml (P<0.05).

According to the results, it can be concluded that S. boulardii cell wall extract could be a candidate for IL-13- and IL-17-induced Aspergillus-mediated allergy and asthma therapies. Nevertheless, future studies need to be conducted on the safety of S.boulardii cell wall extract in vivo and its effects on other arms of allergic hypersensitivity.

The current investigation aimed to study the effect of certain parameters, including IL-17A, creatinine, lymphocytes, and basophils, on patients suffering from systemic lupus erythematosus-induced renal failure.

This clinically controlled study involved 105 patients (35 males, 70 females) confirmed to have systemic lupus erythematosus in Thi-Qar Province, Iraq. Researchers collected 5ml of venous blood from 50 healthy controls to conduct immune tests (to determine the levels of IL-17A by ELISA), biochemical tests (to measure creatinine levels by colorimetric method), and CBC tests (to assess lymphocyte and basophil counts). The data was analyzed using SPSS 23 software using the Chi-square and Welch's T-tests.

The systemic lupus erythematosus rate was 83%, and females exhibited a higher infection rate (66.7%) than males (33.3%). Significant differences between the sexes were identified. Also, significant increases were seen in the levels of IL-17A and creatinine in patients compared to healthy controls. Furthermore, females aged 13-45 were more affected than males. While levels of IL-17A and creatinine were elevated in some patients, lymphocyte and basophil levels were decreased in others compared to the healthy group.

IL-17A, creatinine, lymphocytes, and basophils play a fundamental role in the pathogenesis of systemic lupus erythematosus.

T-helper 17 (Th17) cell response is engaged in the onset of allergic rhinitis (AR). Moreover, interleukin (IL)-38 is thought to be involved in inhibiting cytokine secretion in the Th17 pathway. To evaluate the regulatory function of IL-38 on abnormal Th17 responses in Chinese patients with AR. Forty-five participants, divided into an AR group (n=25) and a control group (n=20), were recruited for the study. In addition, the expression of IL-38 and Th17-related cytokines was measured as well as the Th17 cell count in participants. By implementing recombinant IL-38 (rIL-38), the intervention of human peripheral blood mononuclear cells (PBMCs) was performed. Then, flow cytometry, polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA) were used to detect theTh17 milieu. The expression of IL-38 in the AR group notably reduced compared with that in the control, whereas Th17 cell frequency and the expression levels of its transcription factor (RORC) and cytokines (IL-17A and IL-23) increased. The differentiation and immune function of Th17 cells in PBMCs were inhibited by rIL-38. Th17 responses are inhibited by IL-38 in patients with AR. Therefore, the obtained findings indicate that IL-38 is a potential therapeutic target for Chinese patients with AR.

Brucellosis is a zoonotic disease in humans and animals and is a worldwide public health problem. Changes in inflammatory cytokines levels might be deployed as markers for diagnosing infectious diseases from non-infectious medical conditions. This study aimed to evaluate the relationship between serum levels of interleukin-17 (IL-17) and transforming growth factor-beta (TGF-β) in pediatric brucellosis.

The present case-control study included 40 brucellosis patients and 40 matched healthy controls. Serum levels of inflammatory cytokines were measured by ELISA, and the independent student t-test was used to compare the levels in the brucellosis and healthy group. Serum cytokine levels before and after treatment were compared by the paired samples t-test.

The serum TGF-β level was significantly lower in the patients compared to the control group (90.21 ± 24.44 vs. 125.63 ± 23.28 pg/mL, P<0.nv001), and the serum interleukin-17 level was significantly higher in the case group (83.74 ± 23.57 vs. 25.95 ± 17.80 pg/ml, P<0.001). After treatment, serum IL-17 levels significantly decreased in the case group.

In brucellosis patients, the serum IL-17 levels decreased significantly, whereas TGF-β increased significantly in these patients. Hence, the serum levels of these inflammatory cytokines can be indicators for diagnosing pediatric brucellosis.

Chronic periodontitis (CP) is characterized by an immune response, leading to the destruction of periodontal supporting tissue. The effect of inflammatory and genetic factors on periodontitis has been evaluated previously. The interleukin (IL‑17) as an inflammation regulator seems to play a critical role in periodontitis pathogenesis. Here, in the current study, we aimed to investigate the association of ‑197 G > A (rs2275913) IL‑17 gene promoter polymorphism with generalized severe CP in an Iranian population.

In this case–control study, a total of 54 patients with periodontitis and 118 normals were enrolled. The polymerase chain reaction‑restriction fragment length polymorphism technique was applied to detect IL‑17 promoter rs2275913 genotypes in association with the susceptibility to severe CP. Chi‑square test or Fisher’s exact test was employed to compare genotype frequencies between groups. P < 0.05 were considered statistically significant.

The distribution of genotypes and alleles was in Hardy–Weinberg equilibrium. Although no significant association was observed between the risk of periodontitis and genotype frequencies under any of the inheritance models, the GG genotype was higher in healthy controls, while the AG genotype was more frequently observed in patients under the codominant model ([odd ratio [OR] = 2.14, 95% confidence interval (CI) (1.01–4.53), P = 0.13]). The frequency of AG‑AA genotype was higher in patients under dominant inheritance model ([OR = 1.92, 95% CI (0.94–3.93), P = 0.068]), while GG‑AA and AG genotypes were higher in healthy controls under over dominant model (OR = 0.1.95, 95% CI [0.98‑3.86], P = 0.055).

The results of this study showed that the presence of allele A and AG genotypes could be considered possible factors in increasing the risk of developing CP, although the differences of allele and genotype frequencies were remarkable but not statistically significant between the two groups.

This study aimed to investigate the effect of 8 weeks of strenuous endurance training (ET) with ginger extract supplementation on resting levels of serum interleukin-17 (IL-17) and total antioxidant capacity (TAC) of muscle in male Wistar rats.

Forty rats were divided randomly into five groups: control (n = 8), Sham (n = 8), zingier (n = 8), ET (n = 8), and ET + zingiber (n = 8). The training protocols consisted of ET on a treadmill for 8 weeks (5 days a week). Ginger extract (100 g/kg body weight) was injected subcutaneously in the ginger group and ET + zingiber group for 8 weeks (3 days a week) from the beginning of the second week. Forty-eight hours after the last training session and 4 hours of overnight fasting, blood and tissue samples were collected. The serum concentration of IL-17 was measured using the ELISA method by BT kit, China. TAC concentration of muscle tissue was measured using the colorimetric method with ZELBIO kits, Germany. One-way analysis of variance (ANOVA) and Scheffe’ post hoc tests were used to analyze the data.

This study showed that after 8 weeks, serum levels of IL-17 were significantly higher in the ET groups than in the control group (P < 0.05). Also, serum IL-17 was significantly decreased in the ET + ginger group than the endurance group (P < 0.05). However, there was no significant difference in TAC of muscle tissue in the ET, ginger, and ET + ginger groups than the control group (P < 0.05).

This study demonstrated that 8 weeks of endurance training, combined with ginger supplementation, significantly reduced the inflammatory marker of IL-17. Therefore, ginger supplementation with endurance training seems to have a protective role against oxidative and inflammatory factors.

Regular physical activities may have effect on the course of chronic kidney disease (CKD). Here, we aimed to ascertain the changes of serum interleukin-17 (IL-17) following eight weeks of aerobic training in CKD patients.

The CKD patients referred to Zahedan Edalat Clinic and Ali-Ibn Abi Talib hospital in Zahedan city (Iran) were enrolled. Sixty patients aged between 30 and 50 years old were chosen by a random method and assigned into the control and intervention groups (each group constituted 30 people). In this study, aerobic exercises were performed at 50%–80% of the maximal heart rate. Peripheral blood was obtained one day before the beginning of exercise and one day after the end of the intervention. Serum IL-17 level was quantified using a commercial specific ELISA kit.

The mean values of IL-17 in CKD patients before and after 8 weeks of aerobic exercise were 1.67 ± 0.403 pg/mL and 1.58 ± 0.170 pg/mL in the intervention group (P value= 0.039) whereas the mean values of IL-17 in the control group before and after the intervention were 1.31 ± 0.529 pg/mL and 1.35 ± 0.505 pg/mL (P value= 0.794).

Eight weeks of aerobic training can significantly reduce serum IL-17, an inflammatory marker, in CKD patients.

Toxoplasma gondii is a zoonotic protozoan with worldwide distribution. Recent studies have shown that the transmission of T. gondii is facilitated by its ability to modify the host’s behavior.

The present study aimed to investigate the effect of T. gondii infection on anxiety in an animal model and determine the levels of cortisol and interleukin-17 (IL-17) in rats.

In this study, 40 rats were randomly allocated to four groups, namely uninfected animals as the control group, infected group, infected and trimethoprim-sulfamethoxazole (TMP-SMX)-treated group, and infected and dexamethasone-receiving group. Thirty days after the infection, the rats were subjected to behavioral tests utilizing the plus maze. The cortisol and IL-17 levels in the serum of the infected rats were measured by the enzyme-linked immunosorbent assay.

The infected rats had a significantly higher number of entries to the open arms, and the mean spent time in the open arms was higher than that of the control group (P < 0.01). The dexamethasone-receiving and TMP-SMX-treated rats had a lower number of entries to the open arms, and the mean spent time in the open arms was less than that of the infected group; however, there were no significant differences in closed arm entries between different groups and the control group. Regarding the total activity, the infected rats had significantly higher values than the controls, dexamethasone-receiving rats, and TMP-SMX-treated rats; nevertheless, the differences were not statistically significant.

In conclusion, it was observed that T. gondii had anxiolytic effects, and IL-17 and cortisol levels increased in the serum of the infected rats.

Nitric oxide gas is important in regulating blood vessel dilation, and consequently, blood pressure. Nitric oxide is continuously produced by endothelial cells known as a compound that plays an important role in the cardiovascular system.  Also, Interleukin-17 (IL-17) is a pro-inflammatory cytokine that can mediate protective innate immunity to pathogens or contribute to the pathogenesis of inflammatory diseases. In the current study, the plasma levels of nitric oxide and serum level of IL-7 were assessed before and after Ramadan month in fasting people, aimed at the scientific investigation of the possible preventing effects of fasting for cardiovascular disease.

The present study 61 fasting man (30-60 years old) were selected and blood samples were collected from each one day before and one day after Ramadan month. Serum levels of Nitric oxide and IL-17 were measured in two groups by ELIZA kits.  

The results of this study shown the serum level of nitric oxide was significantly increased in individuals before and after Ramadan month (from 16.17 ± 1.15 mmol/L to 22.17 ± 1.46 mmol/L, respectively) (P<0.0001). Also, the results indicate that serum level of IL-17 was statistically different in two groups (from 58.25 ± 10.59 pg/ml to 15.98 ± 4.66 pg/ml, respectively) (P<0.0001).

The results of this study showed that nitric oxide and IL-17 levels decreased during the Ramadan month and so it can be concluded the fasting of Ramadan may have positive effects on cardiovascular and immune factors of the body.

Asthma is a common airway inflammation with an intricate underlying mechanism. The role played by circulating miRNAs in asthma remains unclear. In the present study, we aimed to investigate the role of miR-223-3p in leukocytes of asthma and identify the relationship between miR-223-3p and inflammatory cytokines in asthma. Using real-time polymerase chain reaction (RT-PCR), we detected miR-223-3p expression in peripheral blood leukocytes from 23 asthmatic patients and 20 healthy controls. The levels of IFN-γ (Th1 cytokine), IL-4 (Th2 cytokine), IL-17A (Th17 cytokine) in plasma were examined using enzyme-linked immunosorbent assay (ELISA). Analysis of variance (ANOVA) and Spearman’s test was used for statistical analysis. The expression of miR-223-3p in peripheral blood leukocytes was upregulated in the asthmatic patients compared with that in the healthy controls. Increased miR-223-3p expression was associated with forced expiratory volume in 1-second percent predicted (FEV1% predicted). A positive correlation was noted between miR-223-3p and IL-17A. The findings of this study showed that miR-223-3p plays a vital role in the pathogenesis of asthma and can serve as a novel biomarker for asthma.

Multiple Sclerosis is a central nervous system disease which belongs to the category of autoimmune diseases. The prevalence of this disease in Iran is approaching the European level. Astrocyte cells are nerve tissues that regulate the immune system activity by secreting various cytokines such as IL- 17.  The aim of this study was partial purification of toxin from M.eupeus scorpion venom that has immunomodulatory effect on astrocyte cell line (1321N1) In the present study, purified crude venom of M.eupeus scorpion. Size exclusion and reverse-phase high-performance liquid chromatography was used for fractionation. The fractional molecular weight was determined by Using SDS and Tricine electrophoresis, Astrocyte cells (1321N1) were selected as functional cells in testing the immunomodulatory effect of venom. The viability of cells were determined by MTT and LDH assays. Astrocyte cells were activated by lipopolysaccharide and the release of interleukin-17 in activated cells was estimated using ELISA kit.   fraction 331 (F331) from RP-HPLC contain the purified peptide with molecular weight of about 4500 Dalton. When activated cells exposed to purified peptide the rate of interleukin-17 release was found to be 85 pg/ml which is almost similar to un-activated cells (78 pg/ml). However in activated cells by LPS without treatment with purified peptide the rate of IL-17 release was found to be 147 pg/ml which was significantly (p <0.05) higher than control group.   The purified peptide (F331) from   venom of Mesobouthus eupeus can inactivate the astrocyte 1321N1 cells activated by LPS as indicated by decreased secretion of IL-17 from the cells.

We aimed to detect IL-17, MMP-9 and CD23 in serum of patients with colorectal cancer to provide some proper references for diagnosis and treatment of this disease.

Overall, 287 patients with colorectal cancer were collected in the Digestive Surgery Department of Chinese PLA General Hospital, Beijing, China from January 2017 to November 2018 and were used as the study group, meanwhile, 200 people who took physical examination in the same period were used as the control group. They were retrospectively analyzed. The concentrations of IL-17, MMP-9 and CD23 in serum were detected by ELISA 10 d before and after treatment and 30 d after treatment. The relationship between IL-17, MMP-9 and CD23 concentration and clinicopathology was analyzed.

The concentrations of CD23, IL-17 and MMP-9 in peripheral blood of the patients in the study group were significantly higher than those in the control group (P<0.001). IL-17, MMP-9 and CD23 were negatively correlated with treatment time and pathological features in the study group (P<0.001).

The concentrations of IL-17, MMP-9 and CD23 obviously increased in peripheral blood of patients with colorectal cancer, the three were negatively correlated with treatment time and were significantly correlated with TNM staging and differentiation degree of colorectal cancer. It is expected to estimate the illness.