جستجوی مقالات مرتبط با کلیدواژه « islet cells » در نشریات گروه « پزشکی »

Background and Although it is now certain that insulin injection allows us to control the complications resulting from blood sugar imbalances, its accurate control by insulin injection is difficult. Therefore as a logical solution, transplantation of islet cells is globally considered as a treatment and the present study is conducted to verify it. Methods and Materials: After materials preparation, donor tissue was obtained from 6 male wistar rate cage 75-90 days (weight 250-300 gram). Transplantation was done on diabetic rate induced 2-4 weeks earlier by 60 mg/kg streptozotocin intravenous injection. After islet cells transplantation, blood glucose was reduced to normal (145±5 mg/dl) and insulin and C-peptide increased (1.9±0.1 MIU/L and 0.053±0.001 mg/ml respectively). Clinical symptoms of dibetes induction were relieved after transplantation. The technique of islet cells transplantation in the form of encapsulation with the absence of immunological inhibitors to support the graft against the recipient's immunity system is an innovative method to treat type I diabetes.

Background and It is thought that transplantation of islet cells would cure diabetic patients in world. Islet cells transplantation in people suffering from diabetes is of technical interest. A standardized procedure was developed for the preparation of rat islet cell grafts for purification of islet cells. In this process, after collagens digestion of pancreases, islets were isolated and dissociated, then with enzymatic procedure by DNase and trypsin, the islet cells changed in to single cells and these cells were assayed by flow cytometery. Flow cytometery of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles were lost during digestion. Purified endocrine islet cell grafts were prepared by pure beta-cells, with or without endocrine non-beta cells. The purified aggregates were devoid of non-endocrine cells and damaged cells.

Hyperinsulinism is responsible for about 55% of hypoglycemia in children less than 1 year old. In this study pancreas from 8 children with idiopathic hyperinsulinemic hypoglycemia of infancy(HHI) who underwent pancreatectomy in Ali-Asghar Hospital from 10 years ago were examined, using histochemical method and morphometric measurements. The children ranged from 3 to 13 months, and were presented with seizure. They all had positive glucagon test and high serum ins/glu ratio and were unresponsive to medical therapy(Diazoxide, Somatostatin or Hidrocortisone). Sections were studied with particular reference to islet cell distribution in patients and controls. Quantitative assessments were made of islet size and total surface area of pancreas occupied by endocrine tissue. Four patients had diffuse islet cell hyperplasia and others showed multifocal islet cell hyperplasia, islet cell dysplasia, adenoma and normal pancreas, respectively. Immunostaining can show any recapitulation of islet structure or increase of B cells. Furthermore, frozen section study during surgery can help to distinguish different pathologies of HHI.