جستجوی مقالات مرتبط با کلیدواژه "mentha pulegium" در نشریات گروه "پزشکی"
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سابقه و هدف
در سال های اخیر، نوشیدنی های فراسودمند تولید شده با افزودن آنتی اکسیدان ها، ویتامین ها و باکتری های پروبیوتیک توانسته اند جایگزین مناسبی برای نوشابه ها باشند. این تحقیق به منظور ارزیابی خواص آنتی اکسیدانی، محتوای فنلی و خاصیت مهارکنندگی آنزیم آلفاگلوکوزیداز اسانس اسطوخودوس، خالواش، علف لیمو و نوشیدنی فراسودمند تولید شده از اسانس گیاهان منتخب و ارزیابی حسی نوشیدنی مذکور انجام شد.
مواد و روش هاابتدا اسانس گیاهان با دستگاه کلونجر توسط بخار آب استخراج شد و آنالیز ترکیبات تشکیل دهنده اسانس گیاهان با استفاده از دستگاه کروماتوگرافی گازی انجام شد. خواص آنتی اکسیدانی، محتوای فنلی و مهار آنزیم آلفاگلوکوزیداز در اسانس گیاهان مذکور مورد بررسی قرار گرفت و باتوجه به خواص اسانس گیاهان، از بین سه گیاه مذکور، از اسانس گیاهان علف لیمو و اسطوخودوس برای تولید نوشیدنی انرژی زا فراسودمند استفاده شد. سپس آزمایش خواص آنتی اکسیدانی، تعیین محتوای فنلی و ارزیابی حسی (رنگ، بو و مزه) برای نوشیدنی های تولید شده انجام شد.
یافته هانتایج حاصله در بررسی فعالیت آنتی اکسیدانی نشان داد؛ اسانس اسطوخودوس و علف لیمو بالاترین خاصیت آنتی اکسیدانی را دارد. در خصوص محتوای فنل کل مشخص شد که محتوای فنلی در اسانس خالص گیاهان باهم اختلاف معنی دار دارد (05/0<p). بر اساس نتایج حاصله، اسانس گیاه علف لیمو دارای بالاترین محتوای فنلی بود. درصد مهارکنندگی آلفاگلوکوزیداز برای اسانس گیاه علف لیمو، اسطوخودوس و خالواش حدودا 60 درصد بود. نتایج حاصل از بررسی خواص آنتی اکسیدانی و محتوای فنلی و ارزیابی حسی برای نوشیدنی های حاوی اسانس ها نشان داد که به ترتیب نوشیدنی حاوی اسانس علف لیمو و سپس اسطوخودوس دارای بیشترین مقدار ترکیبات فنلی، خاصیت آنتی اکسیدانی و مطلوبیت حسی هستند.
نتیجه گیرینتایج تحقیق نشان داد که اسانس گیاهان علف لیمو و اسطوخودوس ویژگی های حسی و آنتی اکسیدانی نوشیدنی را افزایش دادند و به دلیل افزودن خواص گیاهی خود به نوشیدنی، می توان آن را فراسودمند نامید. همچنین نتایج ارزیابی حسی نشان داد که نوشیدنی های انرژی زای فراسودمند تولید شده از پذیرش خوبی برخوردارند.
کلید واژگان: نوشیدنی انرژی زا فراسودمند, اسانس, علف لیمو, اسطوخودوس, خالواشBackground and ObjectivesIn recent years, super-beneficial drinks produced by adding antioxidants, vitamins and probiotic bacteria can be appropriate alternatives to soft drinks. This research was carried out to assess antioxidant characteristics, phenolic content and alpha-glucosidase enzyme inhibition of lavender, khalowash and lemongrass essential oils and a beneficial drink made from the essential oils of selected plants and was finally assessed by sensory assessment.
Materials and MethodsFirst, essential oils of the plants were extracted and constituent analysis of the essential oils was carried out using gas chromatography. Antioxidant characteristics, phenolic content and alpha-glucosidase enzyme inhibition were investigated in the essential oils of the plants. Based on the characteristics of the essential plants, essential oils of lemon grass and lavender plants were used to produce beneficial drinks.
ResultsResults included antioxidant characteristics of lemongrass, lavender and khalowash essential oils. Regarding the total phenolic content, it was detected that the phenolic content in the pure essential oils of the plants included significant differences (p ˂ 0.05). Based on the results, lemongrass essential oil included the highest phenolic content. Inhibition percentage of alpha-glucosidase for the plant essential oil was nearly 60%. Then, the produced drinks were assessed for antioxidant characteristics and phenolic content and sensory properties (color, odor and taste).
ConclusionResults showed that the essential oils of lemongrass and lavender plants increased general characteristics of the drinks and because of adding their herbal characteristics to the drinks, it can be addressed as beneficial. Sensory assessment results showed that the produced ultra-beneficial drinks were well accepted.
Keywords: Functional drink, Essential oil, Lemon grass, Lavender, Mentha pulegium -
سابقه و هدف
کاربرد پزشکی نانوامولسیون روغن در آب به دلیل خواص ویژه گسترش یافته است. نانوامولسیون ها بر پایه گیاهان دارویی به دلیل روش تهیه ساده و سریع، کارایی بالا، و صرفه اقتصادی مهم هستند.
روش بررسیدر این پژوهش نانوامولسیون روغن در آب حاوی اسانس پونه به عنوان فاز روغنی، عصاره پونه به عنوان فاز آبی، و سورفکتانت اسپن 80 به مدت 30 دقیقه توسط هموژنایزر تهیه شد. خواص نمونه ها مورد بررسی قرار گرفت. کیتوزان به دلیل زیست سازگاری، زیست تخریب پذیری، و خواص ضدباکتری به عنوان ماده زیستی طبیعی جهت اصلاح نانوامولسیون بهینه استفاده شد. نمونه ها با روش های پراکندگی نور دینامیکی (DLS) جهت بررسی اندازه و توزیع اندازه، میکروسکوپ الکترونی عبوری (TEM) برای سنجش اندازه و شکل، و پتانسیل زتا برای تعیین پایداری ارزیابی شدند. فعالیت ضدباکتری، فعالیت ضداکسیدانی، و سمیت سلولی نمونه ها بررسی شد.
یافته هانتایج فعالیت ضدباکتری اسانس، عصاره، و نانوامولسیون ها علیه باکتری گرم منفی اشرشیاکلی (E.Coli) و باکتری گرم مثبت استافیلوکوکوس اوریوس (S.Aureus) نشان داد که این مواد کاندید مناسب بجای داروهای شیمیایی جهت مقابله با سویه ها هستند. فعالیت ضداکسیدانی نمونه ها بر اساس سنجش ترکیبات فنولی تام و مهار ادیکال آزاد مشاهده شد. همچنین نمونه ها سمیت سلولی روی سلول های سالم ندارند.
نتیجه گیریبر اساس نتایج، نانوامولسیون روغن در آب حاوی اسانس به عنوان فاز روغنی و عصاره به عنوان فاز آبی می تواند کاندید مناسبی برای کاربردهای دارویی و پزشکی باشد.
کلید واژگان: نانوفناوری, نانوامولسیون, پونه, عصاره, اسانسMedical Science Journal of Islamic Azad Univesity Tehran Medical Branch, Volume:33 Issue: 4, 2023, PP 355 -364BackgroundThe medical application of oil-in-water nanoemulsion has been expanded due to its special properties. Nanoemulsions based on medicinal plants are important because of the simple and fast preparation method, high efficiency, and economic efficiency.
Materials and methodsIn this study, the oil-in-water nanoemulsion was prepared containing Mentha pulegium essential oil as the oil phase, Mentha pulegium extract as the aqueous phase, and span 80 surfactant for 30 minutes using homogenizer. The properties of samples were investigated. Chitosan was used as the natural biological material to modify the optimal nanoemulsion due to its biocompatibility, biodegradability, and antibacterial properties. The samples were evaluated by methods of dynamic light scattering (DLS) to investigate size and size distribution, transmission electron microscopy (TEM) to measure size and shape, and zeta potential to determine stability. Antibacterial activity, antioxidant activity, and cytotoxicity of the samples were investigated.
ResultsThe results of the antibacterial activity of the essential oil, its extract, and nanoemulsions against gram-negative bacteria escherichia coli (E.Coli) and gram-positive bacteria staphylococcus aureus (S.Aureus) showed that these materials are suitable candidates instead of chemical drugs to deal with strains. The antioxidant activity of the samples was observed based on the measurement of total phenolic compounds and free radical inhibition. Also, the samples are not cytotoxic on healthy cells.
ConclusionBased on the results, oil-in-water nanoemulsion containing essential oil as the oily phase and extract as the aqueous phase can be suitable candidates for pharmaceutical and medical applications.
Keywords: Nanotechnology, Nanoemulsion, Mentha pulegium, Extract, Essential oil -
Background and Objectives
The aim of this study was to investigate the effect of dietary supplementation with Mentha pulegium and Rosmarinus officinalis on the carcass characteristics, physicochemical properties, color, texture and oxidative stability of Japanese quail breast meat.
Materials and Methods120 Japanese quails were divided into 4 treatments and 3 replications including T1: basic diet (control), T2: basic diet + M. pulegium (1.5%), T3: basic diet + R. officinalis (1.5%) and T4: basic diet + M. pulegium (1.5%) + R. officinalis (1.5%).
ResultsThe results showed there was no significant difference in breast and thigh weight, pH, dry matter, ash, cooking loss and vitamin E content among the treatments. Live weight, protein and fat contents, redness (a*), yellowness (b*), phenol and carotenoid contents of all treatments increased significantly (P<0.05) in comparison to control. Supplementation of diet with M. pulegium and R. officinalis individually or in combination significantly reduced TBARS, lightness (L*) and hardness compared to control (P<0.05).
ConclusionsThe results revealed that supplementation of diet with M. pulegium and R. officinalis improved the protein and fat contents, color and oxidative stability of Japanese quail breast meat.
Keywords: Breast meat quality, Dietary supplementation, Mentha pulegium, Oxidative stability, Rosmarinus officinalis -
هدف
علی رغم درمان های متعدد شپش سر هنوز به عنوان یک مشکل بهداشتی خصوصا در دختران دانش آموز می باشد. در این پژوهش تاثیر استفاده موضعی ترکیب جوشانده سیر و پونه کوهی بر شدت آلودگی به شپش سر بررسی شده است.
مواد و روش هامطالعه از نوع کار آزمایی بالینی تصادفی شده، به صورت چند مرحله ای، بر روی 120 دانش آموز دختر مقاطع ابتدایی و متوسطه اول مبتلا به شپش سر شهر گناباد انجام، پس از اخذ رضایت نامه کتبی از والدین و شفاهی از دانش آموزان، تکمیل پرسش نامه دموگرافیک و چک لیست، به یک گروه جوشانده سیر و پونه کوهی و گروه دیگر لوسیون دایمتیکون4% در دو نوبت به فاصله یک هفته داده شد. فراوانی شدت آلودگی قبل، 7 روز بعد و 14 روز بعد از مداخله اندازه گیری شد.
یافته هاشدت آلودگی به شپش در طی انجام مداخلات در هر دو گروه روند کاهشی معنی داری نشان داد (001/0=P)، اما این تفاوت در دو گروه قبل، روز هفتم و روز چهاردهم مداخله معنی دار نبود (05/0<P).
نتیجه گیرینتایج ما نشان می دهند که هر دو جوشانده سیر و پونه کوهی می توانند برای درمان شپش سر استفاده شوند.
کلید واژگان: سیر, درمان های مکمل, دایمتیکون, پونه, آلودگی با شپشKoomesh, Volume:23 Issue: 6, 2021, PP 767 -776IntroductionDespite numerous treatments, head lice are still a health problem, especially in female students. In this study, the effect of topical application of Allium Sativum and Mentha Pulegium on the severity of head lice infection has been investigated.
Materials and MethodsA multi-stage randomized clinical trial study was performed on 120 female primary and secondary school students with head lice in Gonabad. After obtaining written consent from parents and oral consent from students, a demographic questionnaire and a checklist were completed. One group was given a decoction Allium Sativum and Mentha Pulegium and the other group was given 4% dimethicone lotion twice a week. Evaluation was made before, 7 days and 14 days after the intervention.
ResultsThe severity of lice infection during the interventions in both groups decreased significantly (P=0.001), but the frequency of severity of infection before, 7 days after and 14 days after intervention was not statistically significant in the two groups.
ConclusionOur findings suggest that both Allium Sativum and Mentha Pulegium can be used for the treatment of head lice infection.
Keywords: Garlic, Complementary Therapies, Dimethicone, Mentha Pulegium, Lice Infestations -
Purpose
In this study, leaf/flower aqueous extract of medicinal plant species Mentha pulegium was used to synthesize ZnO and CuO nanoparticles (NPs) as a cost-effective, one-step, and eco-friendly method.
MethodsPhysicochemical properties of both metal oxide NPs (MONPs) were determined by UV-Vis spectroscopy, X-Ray Diffraction (XRD), Fourier-Transform Infra-Red (FTIR) spectroscopy, Scanning Electron Microscope (SEM) and Energy Dispersive X-ray (EDX) techniques.
ResultsPhytofabricated ZnONPs and CuNPs illustrated 65.02±7.55 and 26.92±4.7 nm with antibacterial activities against antibiotic-resistant Escherichia coli and Staphylococcus aureus. Higher antibacterial activities were observed for CuONPs compared with ZnONPs.
ConclusionLarge surface area and more reactivity resulted from smaller size as well as higher production of reactive oxygen species (ROS) were considered to antibacterial efficiency of CuONPs against antibiotic-resistant E. coli and S. aureus.
Keywords: Phytosynthesis, Metal oxide NPs, Mentha pulegium, Antibiotic resistance, Antibacterial activities -
Every year, millions of people worldwide get sick with gastrointestinal diseases such as heartburn. Certain herbs contribute to the alleviation of heartburn, nausea, and improvement of digestion. Moreover, these herbs do not have as many side effects as synthetic drugs. As a health problem and one of the challenging issues in medical sciences, heartburn is common in children and adults worldwide. Hence, in the present study, we tried to report medicinal plants used in cultures and traditions of different regions of Iran to treat heartburn in children and adults. In this review study, articles of Iranian ethnobotanical sources were searched with the keywords of ethnobotanics, heartburn, children, adult, medicinal plants, and Iran. Journal articles published from 2010 to 2019 in several Iranian and International databases, including ISI Web of Science, PubMed, Scopus, ISC, Magiran, were searched to find relevant articles and information. Anethum graveolens L., Punica granatum L., Mentha pulegium, Thymus kotschyanus Boiss. & Hohen., Achillea millefolium, Ocimum basilicum, Nigella sativa, etc., are the plants used in different parts of Iran to treat heartburn.
Keywords: Digestive problem, Heartburn, Anethum graveolens, Mentha pulegium, Medicinal plants EthnobotanyIran -
Background
Among important fungi associated with foods are Aspergillus spp., Penicillium spp., and Geotrichum spp. In this study, we evaluated antifungal effects of Essential Oils (EOs) of Zataria multiflora, Mentha pulegium, and Mentha piperita.
MethodsAntifungal properties of EOs of M. piperita, M. pulegium, and Z. multiflora against Aspergillus spp., Penicillium spp., and Geotrichum candidum were determined by agar well diffusion and broth macrodilution method. Data were analyzed by SPSS 20.
ResultsAmong three studied plant EOs, Z. multiflora EO had the strongest antifungal activity (p<0.05) on tested fungi; so that the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were 0.01 and 0.3% for G. candidum, 0.005 and 0.3% for Penicillium spp., and 0.1 and 0.3% for Aspergillus spp.
ConclusionAll three studied plant EOs showed antifungal activities. However, as Z. multiflora EO showed the most antifungal effect, it could be specially suggested as natural powerful antifungal preservatives in the food industry.
Keywords: Antifungal Agents, Thymus Plant, Mentha pulegium, Mentha piperita -
زمینه و هدف
مصرف روز افزون آنتی بیوتیک ها علیه عفونت ناشی از میکروارگانیسم ها سبب افزایش مقاومت دارویی شده است. این امر سبب شده تا پژوهش های وسیعی بر روی ترکیبات گیاهی با خاصیت ضدمیکروبی و اثربخشی بیشتر به عنوان جایگزین آنتی بیوتیکی صورت گیرد. هدف از این تحقیق تعیین و ارزیابی فعالیت ضدباکتریایی عصاره های دارچین، زنجبیل، چای ترش، زرشک و پونه کوهی بر تعدادی از باکتری های استاندارد استافیلوکوکوس ساپروفیتیکوس، استافیلوکوکوس اور یوس، انتروکوکوس فکالیس، سودومونا آیروژینوزا، اسینتوباکتربومانی، کلبسیلا پنومونیه واشریشیاکولی بود.
روش بررسیاین یک مطالعه تجربی می باشد که در سال 1389 در دانشگاه علوم پزشکی کردستان انجام شد. عصاره الکلی گیاهان دارچین، زنجبیل، چای ترش، زرشک وپونه کوهی با روش ماسراسیون تهیه شد. عملکرد ضد باکتریایی عصاره ها بر روی چندین رده استاندارد باکتریایی با روش چاهک گذاری پس از سه بار تکرار انجام و میانگین قطر هاله عدم رشد اندازه گیری و ثبت شد. سپس حداقل غلظت مهاریMIC با روشرقیق سازی با میکروپلیت Broth Microdilution تعیین شد. میانگین قطر هاله عدم رشد با استفاده از آزمون آماری مقایسه میانگین ها به روش دانکن تجزیه و تحلیل شد.
یافته ها :
در این تحقیق بیشترین اثر ضدباکتریایی مربوط به عصاره زرشک و زنجبیل بر روی استافیلوکوک ساپروفیتیکوس به ترتیب با قطرهاله عدم رشد 25 و23 میلی متر بوده است (05/0>p). عصاره چای ترش بر روی استافیلوکوک ساپروفیتیکوس و عصاره پونه کوهی برروی اشیریشیا کولی و استافیلو کوک ساپروفیتیکوس دارای بیشترین اثر ضدباکتریایی به ترتیب با قطر هاله عدم رشد 16، 13و 13 میلی متر بوده اند (05/0>p). بیشترین اثر ضدباکتریایی دارچین بر استافیلوکوک اوریوس با قطر هاله عدم رشد 19 میلی متر بوده است. حداقل غلظت بازدارندگی از رشد مربوط به عصاره گیاهان زرشک و زنجبیل علیه استافیلوکوکوس ساپروفیتیکوس با 1/3 میلی گرم بر میلی لیتر و پایین تر از سایر انواع عصاره ها مشاهده شد. باکتری سودوموناس ایروژینوزا نسبت به اکثر عصاره گیاهان مورد بررسی مقاومت نشان داد (05/0>p).
نتیجه گیری :
نتایج این بررسی نشان داد عصاره های این مطالعه به ویژه عصاره زرشک خاصیت ضدباکتریایی موثری بر روی اکثر سویه های مورد بررسی دارد و برای نشان دادن اثرات بهینه ضدباکتریال عصاره های مورد مطالعه، بررسی بر روی حیوانات آزمایشگاهی و تعیین اثرات بالینی آنها پیشنهاد می شود.
کلید واژگان: آنتی باکتریال, دارچین, زنجبیل, چای ترش, زرشک, پونه کوهی, باکتری های گرم مثبت, باکتری های گرم منفیArmaghane-danesh, Volume:25 Issue: 6, 2021, PP 717 -730Background & aimIncreasing use of antibiotics against infection caused by microorganisms has increased drug resistance. This has led to extensive research on plant compounds with antimicrobial properties and greater effectiveness as an alternative to antibiotics. The purpose of this study was to evaluate antibacterial activity the five Medicinal plant extracts CinnamomumVerum, Zingiber officinale, Hibiscus sabdariffa, Berberis vulgaris, and Mentha pulegium against standard bacteria: Staphylococcus aureus, Staphylococcus saprophyticus, Entrococcous fecalis, Pseudomonas aeroginosa,Acintobacter bumani, Klebsiella pneumoniae, and Esherishia coli.
MethodsThe present experimental study was conducted in 2020 at Kurdistan University of Medical Sciences, Iran. The ethanol extract of the Cinnamomum verum, Zingiber officinale, Hibiscus sabdariffa, Berberis vulgaris and Mentha pulegium were prepared by maceration method.The antibacterial activity of the extracts was performed on several standard bacterial strains by agar well diffusion method after three replications and diameter of the zone of inhibition was measured and recorded. Then MIC (minimum inhibitory concentration) with Broth microdilution was determined. Mean growth zone diameter was analyzed by Duncanchr('39')s comparison test at the 5% level.
ResultsIn the present study, the highest antibacterial effect was related to Berberis vulgaris and Zingiber officinale extracts on S. saprophyticus with growth inhibition drops of 25 and 23 mm, respectively (p <0.05). Hibiscus sabdariffa extract on S.saprophyticus and Mentha pulegium extract on E. coli had the highest antibacterial effect with a growth halo diameter of 16, 13 and 13 mm, respectively (p <0.05). The highest antibacterial effect of cinnamon was on Staphylococcus aureus with a diameter of 19 mm. The lowest growth inhibitory concentration was observed for Berberis vulgaris and Zingiber officinalis extracts against S. saprophyticus with 3.1 mg / ml and lower than other types of extracts. P. aeruginosa showed resistance to most plant extracts (p<0.05).
ConclusionThe results of the present study revealed that the plant extracts specially Berberis vulgaris had effective antibacterial activity on most of the bacterial strains. In order to show the optimal antibacterial effect of the studied extracts, it is suggested to study on the animal models and determine their clinical effects.
Keywords: Antibacterial Activity, Zingiber officinale Hibiscus sabdariffa, Berberis vulgaris, Cinnamomum verum, Mentha pulegium, Gram- Positive bacteria, Gram -Negative bacteria -
IntroductionRecent researches have shown that many plant Essential Oils (EOs) have a high potential for controlling agricultural pests and can be used as precursors for synthesis of new pesticides. The major limitations for the use of these compounds are rapid evaporation, poor water solubility, and aptitude for oxidation. The aim of this study was to prepare and characterize nanoliposome containing EOs of Mentha pulegium and Ferula gummosa and fumigant toxicity of nanoliposome containing M. pulegium EOagainst T. castaneum.Materials and MethodsIn this study, nanoliposome containing EOs of M. pulegium and F. gummosa were prepared using heating method and its physicochemical properties were evaluated. Also, the impact of fumigant toxicity of M. pulegium EONanoliposome on M. castaneum was investigated.ResultsResults showed that mean (±SD) particles of nanoliposomecontaining M. pulegium and F. gummosa EOs were 345±3.2 and 309±1.67 nm and their encapsulation efficiency were 99.38±0.24% and 96.41±0.26, respectively. The kind of EOs had no significant effect on the physicochemical property of nanoparticles. At the end of 24 h, the release percentage of EOs of nanoliposomes of M. Pulegium and F. gummosa were 46% and 33 %, respectively. The estimated LC50 values for nanoliposome and crude Eos of M. Pulegium against T. castaneum were36.53 and 75.23 µI/I air, respectively.ConclusionsThe results of the current research showed that release and stability of EOs were significantly affected when change to nanoliposome particles. Also, M. pleugium EO nanoliposome showed enhancing fumigant toxicity against T. castaneum in comparison with the crude EO of this plant.Keywords: Essential oil, Nanoliposome, Mentha pulegium, Ferula Gummosa, Heating Method, pesticide
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Background
Hydatidosis is a common disease of both humans and animals, resulting from infection with the larvae of Echinococcus granulosus tapeworm. The aim of this study was to investigate the antiparasitic (protoscolicidal) activities of three essential oils in vitro.
MethodsThis study was designed to evaluate the biochemical composition and in vitro antiparasitic effects of Zataria multiflora, Origanum vulgare and Mentha pulegium essential oils. Gas chromatography was performed to identify the main components of the herbal oils. To determine the antiparasitic properties of the essential oils, live protoscoleces from hydatid cysts were exposed to three concentrations of the herbal oils and were incubated at 37°C for 5, 10, 15, 20 or 25 minutes.
ResultsThe biochemical analysis of these oils indicated that carvacrol and thymol were the major compounds of the Zataria oil. Further, carvacrol and thymol in Origanum essential oil and pulegone and piperitone in Mentha oil were the major compounds. The quickest and slowest antiparasitic effect was achieved from Zataria and Origanum (10%) or from Zataria (0.6%), respectively. The statistical analysis showed a significant difference between the mortality rate of protoscoleces exposed to 0.6% and 1% concentrations, respectively, of Zataria and Origanum at the predetermined exposure times (P<0.05). The three concentrations of Mentha had the same significant statistical differences (P<0.05).
ConclusionEssential oils, Zataria multiflora, Origanum vulgare and Mentha pulegium had significant protoscolicidal activities that were dependent on the concentration of the oils and the exposure times.
Keywords: Protoscoleces, Zataria multiflora, Origanum vulgare, Mentha pulegium, Micro-emulsion, Macro-emulsion -
Background and Purpose
Candida albicans is an important microorganism in the normal flora of a healthy subject; however, it has an expedient pathogenic character that induces hydrolytic virulence. Regarding this, the present study aimed to find an in vitro alternative that could reduce the virulence of this yeast.
Materials and MethodsFor the purpose of the study, the effect of amphotericin B (AmB) combined with the extract of Traganum nudatum (E1) or Mentha pulegium (E2) was evaluated against the hydrolytic activities of esterase, protease, and phospholipase. This effect was determined by calculating the minimum inhibitory concentration (MIC), used to adjust the extract/AmB mixtures in culture media.
ResultsThe evaluated Pz values, which corresponded to the different enzymatic activities, showed a decrease in the hydrolytic activities of C. albicans strains after the addition of E1/AmB and E2/AmB combinations at descending concentrations (lower than the obtained MICs).
ConclusionBased on the findings, it would be possible to reduce the pathogenesis of this species without destabilizing the balance of the flora.
Keywords: Amphotericin B, candida albicans, hydrolytic enzymes, Mentha pulegium, Traganum nudatum -
Background and objective
In recent years, with the increase in resistance due to the indiscriminate use of synthetic antibiotics, it seems necessary to find alternative drugs that have both antibacterial properties and have the least side effects for humans. The purpose of this study is to review the antibacterial properties of some medicinal plants.
Material And MethodIn this review study, the content related to the antibacterial properties of Thymus vulgaris, Mentha pulegium, Crocus sativus, and Salvia officinalis were studied within Magiran, SID, PubMed, MEDLINE, Science Direct, Cochrane Library, Google Scholar, EMBASE, and Scopus databases from 1981 to 2019. Previously published specialized articles and systematic meta-analysis were used as a supplementary source for identifying relevant articles. Finally, data from 46 articles were pooled and analyzed.
ResultExtracts and essential oils of Thymus vulgaris, Mentha pulegium, Crocus sativus, and Salvia officinalis had a good antibacterial properties against a variety of pathogenic bacteria and their infections.
ConclusionAccording to the results of this study, the studied plants can be considered as a suitable option for treating infections caused by pathogenic bacteria and helping to Return the sensitivity of antibiotics in these bacteria, and this requires more comprehensive research on medicinal plants.
Keywords: Antibacterial, Medicinal plants, Thymus vulgaris, Mentha pulegium, Crocus sativus, Salvia officinalis -
In recent years, green synthesis of nanoparticles has attracted a great attention because of medicine and biological applications. In this work, bismuth oxide nanoparticles (Bi2O3 NP) was prepared via green synthesis using mentha pulegium aqueous extract after 24 h at 90°C. The product was characterized by ultraviolet-visible (UV-VIS) spectrophotometer, Fourier transform infrared (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and diffuse reflection spectroscopy (DRS). The antibacterial activities of the samples were determined against Salmonella and Escherichia coli (E.Coli) as Gram-negative bacterial and Staphylococcus aureus (S.aureus) as Gram-positive bacterial. The objectives of this study were the green synthesis of bismuth oxide nanoparticles using aqueous extract with a good potential for UV blocking and antibacterial activity. Based on the obtained results, Bi2O3 NPs can have a good candidate for different applications.
Keywords: Green synthesis, bismuth oxide, Nanoparticles, mentha pulegium, Extract -
Background and objectives
Leishmaniasis is a tropical disease caused by protozoan parasites from the genus Leishmania. In this study, we aimed at investigating the in vitro anti-leishmanial effect of essential oils of Rosmarinus officinalis, Mentha pulegium, Foeniculum vulgare, Lippia citriodora and Pelargonium graveolens.
MethodsThe essential oils were prepared from freshly dried and powdered plants with steam-distilled water. Iranian strain of Leishmania promastigotes was cultured in RPMI medium and the inhibitory effects of different concentrations (25, 32, 62.5, 125, 250, 500 and 1000 μg/ml) of the essential oils were investigated at 24, 48 and 72 hours. The number of live parasites before and after treatment with the essential oils was counted by trypan blue 10% staining and using neobar lam.
ResultsThe essential oils significantly decreased the number of promastigotes in a dose-dependent manner (P<0.05). However, the inhibitory effects of F. vulgare and R. officinalis essential oils were more profound compared to other essential oils. Moreover, concentrations of 500 and 1000 μg/ml of these two essential oils exerted equal and more anti-leishmanial potency compared to glucantime, the first-line drug used for treatment of leishmaniasis.
ConclusionBased on the results, it is recommended to evaluate the in vivo anti-leishmanial effects of the tested essential oils, particularly F. vulgare and R. officinalis.
Keywords: Rosmarinus officinalis, Mentha pulegium, Foeniculum vulgare, Lippia citriodora, Pelargonium graveolens, Leishmania -
نشریه گیاهان دارویی، پیاپی 72 (پاییز 1398)، صص 274 -287مقدمه
یکی از اهداف درمان علم پزشکی، ترمیم زخم در زمان کوتاه تر، با عوارض جانبی کمتر می باشد. اهمیت و اثربخشی استفاده از گیاهان دارویی در سالیان اخیر افزایش یافته است.
هدفمطالعه حاضر جهت بررسی اثر سینرژیک مصرف موضعی اسانس نعنا و عسل در ترمیم پذیری بستر زخم باز پوستی رت انجام شده است.
روش بررسیاین مطالعه تجربی روی 75 سر رت نژاد Wistar انجام گرفت. تحت بیهوشی، زخمی مربع به ابعاد 15 × 15 میلی متر ایجاد شد. برای توزیع موش ها روش تصادفی اعمال شد. سطح زخم ها در گروه های تجربی روزانه یک بار با نعنا، عسل، ترکیبی از هر دو و پماد فنی توئین پوشانیده شد. درگروه شاهد هیچ گونه تیماری انجام نشد. در بررسی ماکروسکوپی و اندازه گیری سطح زخم، در روزهای 1، 4، 7، 10 و 14 ارزیابی شد. در بررسی های هیستوپاتولوژیکی ، یک نمونه از بستر زخم از تمام گروه های مورد مطالعه برداشته شد و مورد پردازش بافتی قرار گرفت. برای بررسی اختلاف معنی داری بین گروه ها آنالیز واریانس یک طرفه و یا دوطرفه استفاده شد. جهت آنالیز داده ها از نرم افزار SPSS نسخه ی 21 استفاده شده و نیز مقدار 05/0 P < از نظر آماری معنی دار درنظر گرفته شد.
نتایجدر گروه درمانی ترکیب عسل و نعنا در مقایسه با گروه های دیگر (درصد بهبودی بیشتر و مساحت زخم کمتر) اثربخشی بر روی تسریع ترمیم زخم، به طور معنی داری بیشتر بود (001/0 < P).
نتیجه گیری:
مصرف ترکیب اسانس نعنا و عسل در ترمیم پذیری زخم باز پوستی موثر بوده و باعث تسریع ترمیم زخم شده است.
کلید واژگان: ترمیم زخم پوستی, سینرژیک, عسل, نعناBackgroundOpen skin lesions cause many problems for the health and the economy of societies in recent years, importance and effectiveness of traditional and complementary medicine have been increased.
ObjectiveSo this study was performed to investigate the synergistic effect of topical use of Mentha pulegium essential oil and honey in healing of cutaneous ulcers in rats.
MethodThis experimental study was performed on 75 healthy wistar male rats. Under anesthesia, a 1.5 * 1.5 mm full thickness square wound was created. In the experimental groups, the surface of the wounds was covered once daily with peppermint, honey, a combination of them and phenytoin ointment. No treatments were performed on the wounds of the control group. For histological studies, using staining (H & E) wound surface and wound healing were evaluated. Data were analyzed by using one-way ANOVA with post hoc Tukey test and P < 0.05 was significant.
ResultsThe results of microscopic study showed histological parameters in wounds bed in the experimental group were significantly different from the control group. Generally, rats treated with honey or mint, or the combination of them, had higher percentage of wound healing and less wound surface, than negative and positive controls groups (P<0.05). Also, combination of honey and peppermint, was more effective than them alone.
ConclusionThe results indicated percentage of wound healing and wound surface, topical use of Mentha pulegium essential oil and honey is effective in healing rat skin ulcers. Also, the synergistic effect of these two agents to accelerate wound healing has been proved.
Keywords: Mentha pulegium, Honey, Skin wound healing, Synergistic effect -
زمینه و هدف
اضطراب یکی از شایع ترین اختلالات روانی است که باعث کاهش کیفیت زندگی و اختلالات مزمن می گردد. با توجه به عوارض جانبی بیشتر داروهای شیمیایی این مطالعه با هدف بررسی اثرعصاره هیدروالکلی برگهای پونه بر اضطراب در موش های صحرایی نر انجام گردید.
روش تحقیقدر این مطالعه تجربی از 24 سر موش صحرایی نر استفاده شد. حیوانات به 4 گروه 6 تایی شامل گروه های کنترل (فاقد تیمار) و سه دسته ی تجربی دریافت کننده ی دوز های 400، 800، 1600 میلی گرم بر کیلوگرم وزن موش عصاره ی هیدروالکلی پونه تقسیم گردیدند. عصاره نیم ساعت قبل از آزمون به صورت داخل صفاقی تزریق شد. جهت سنجش اضطراب از ماز صلیبی مرتفع استفاده شد مدت زمان و تعداد دفعات حضور موش ها در بازو های باز و بسته ماز ثبت گردید. نتایج با استفاده از آزمون های آماری تجزیه واریانس یک طرفه و تست پیگیری توکی مورد ارزیابی قرار گرفتند.
یافته هامیانگین درصد تعداد ورود به بازوی باز و مدت حضور در آنها در موشهایی که در عصاره را دریافت داشتند نسبت به گروه کنترل به صورت معنی دار افزایش(P<0.05) و مدت حضور را بازوی بسته ماز کاهش(P<0.05,0.01) یافت.
نتیجه گیریبرطبق این یافته ها عصاره هیدروالکلی برگ پونه موجب کاهش اضطراب در موش های صحرایی شده و ممکن است این اثر را بر انسان هم داشته باشد که نیاز به مطالعه دارد.
کلید واژگان: پونه, اضطراب, ماز صلیبی مرتفع, موش صحرایی نرBackground and AimAnxiety is one of the most common psychiatric disorders that leads to poor quality of life and chronic disorders. Regarding the side effects of most chemical drugs, this study was conducted to evaluate the effect of hydroalcoholic extract of Mentha pulegium leaves on anxiety in male rats.
Materials and MethodsIn this experimental study, 24 male rats were used. Animals were divided into 4 groups (n=6), including Control groups (untreated) and three experimental groups receiving doses of 400, 800, 1600 mg/kg/w of Mentha pulegium leaves hydroalcoholic extract. The extract was injected intraperitoneally half an hour before the test. Anxiety was assessed by using elevated plus maze. The duration and frequency of the presence of the rats in open arms and closed arms of maze were recorded. Data were analyzed by one-way ANOVA and Tukey post-hock tests.
ResultsThe mean percentage of open arms and their duration was significantly increased in rat receiving the extract compare than control (P <0.05), and the duration of presence in closed arm was reduced by the extract (P <0.05, 0.01).
ConclusionAccording to these findings, Pune's hydroalcoholic extract reduces anxiety in rats and may have this effect on humans’ witch it need to be studied
Keywords: Mentha pulegium, Anxiety, Elevated Plus Maze, Rat -
BACKGROUNDBurn is one of the most common injuries and the 4th common cause of trauma globally. This study was designed to evaluate the effect of the Mentha pulegium extract on second degree burn injuries in rats assessing histopathologic and macroscopic.METHODSM. Pulegium extract dressings was used as a treatment to deep dermal contact burns in rats, compared with two control groups of vaseline dressing and simple dressing by normal saline. After creating second-degree burn on the dorsum of rats, the treatments were applied for 15 min in three groups. Wound dressings were performed on days 1, 3, 7, 14 and 21st and at the same time macroscopic assessment was performed using a digital camera and software processing of photos. Pathologic evaluation of skin specimens was undertaken on days 1, 3,7, 14 and 21st. Wound healing parameters such as epithelialization, angiogenesis, granulation tissue formation, inflammatory cells were compared between these 3 groups.RESULTSApplication of M. Pulegium extract on second degree burn wounds significantly decreased burn surface area and increased fibroblasts in comparison to simple dressing. There was not any statistically significant relationship between M. Pulegium extract treated group and vaseline treated or simple dressing groups on other wound healing parameters.CONCLUSIONThis study delineated that M. Pulegium extract had a positive effect on healing process of second degree burnsKeywords: Mentha pulegium, Burn, Wound, Healing, Rat
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زمینه و هدفآفلاتوکسین ها سموم قارچی طبیعی هستند که توسط گونه های قارچ آسپرژیلوس تولید شده و می توانند مسمومیت،نکروز بافتی وسرطان کبد ایجاد کنند. هدف از این مطالعه، امکان سنجی کنترل تولید آفلاتوکسین B1 قارچ آسپرژیلوس فلاوس توسط عصاره ها و اسانس های گیاهی می باشد.مواد و روش هاعصاره های آبی با کمک روش حرارت دهی مستقیم و اسانس با دستگاه کلونجر، تهیه گردیدند. فعالیت ضدقارچی اسانس و عصاره آبی مرزه و پونه در غلظت های کاهشی متوالی به روش های انتشاردیسک و میکروپلیت دایلوشن تعیین گردید. سپس در غلظت های کمتر از حداقل غلظت ممانعت از رشد، اثر اسانس و عصاره آبی پونه و مرزه بر کنترل میزان تولید آفلاتوکسین B1، با روش HPLC مورد بررسی قرار گرفت.یافته هابیشترین هاله ممانعت از رشد قارچ مربوط به اسانس10 درصد مرزه و عصاره آبی آن به ترتیب با قطر هاله 26 و 12 میلی متر بود. این مقادیر برای اسانس10 درصد و عصاره آبی پونه به ترتیب 18 و 8 میلی متر بود. MIC عصاره آبی مرزه و پونه به ترتیب 031/0و063/0 و اسانس 1 درصد مرزه و پونه به ترتیب 039/0و 078/0 میلی گرم برمیلی لیتر بود. میزان آفلاتوکسین B1 تولید شده در معرض اسانس مرزه با غلظت های 1 ،2 و10 درصد به ترتیب113،122و134 واحد در بیلیون و درمعرض اسانس پونه با همین غلظت ها به ترتیب 163، 168 و 171 واحد در بیلیون بود. عصاره آبی 1 درصد مرزه تولید سم را به مقدار 1/58 درصد و عصاره آبی 1 درصد پونه تولید سم را به مقدار 6/39 درصد کاهش داد.نتیجه گیرییافته های این تحقیق نشان داد که دو گیاه مرزه و پونه دارای توانایی ممانعت از رشد قارچ آسپرژیلوس فلاوس و کنترل تولید آفلاتوکسین B1 در غلظت های پایین می باشند. این محصولات گیاهی جهت مطالعات بیشتر برای کنترل این قارچ پیشنهاد می شوند.کلید واژگان: عصاره آبی, اسانس, مرزه, پونه, آفلاتوکسین B1, آسپرژیلوس فلاوسBackgroundAflatoxins are natural fungal toxins produced by Aspergillus species such as A. flavus. The toxins are poisoning and can cause tissue necrosis and liver cancer. The aim of this study was to determine the control of Aflatoxin B1 production by extracts and essential oils.Materials And MethodsAqueous extracts were prepared by heating and essential oil by Clevenger's apparatus. Antifungal activity of essential oil and aqueous extract of Mentha pulegium and Satureja hortensis were determined by disc diffusion and microplate dilution methods. Production control of Aflatoxin B1 was investigated with concentrations under MIC(Minimum inhibitory growth concentration) of two materials and were determined by HPLC method.ResultsThe most zone of inhibition was 10% belonging to Satureja essential oil and its aqueous extracts with diameters of 26mm and 12mm, respectively. These values for Mentha extract and 10% essential oil were 18mm and 8mm respectively. MIC of the aqueous extract of Satureja and Mentha were 0.031 and 0.063mg/ml respectively, and 1% essential oil of two materials was 0.039 and 0.078 mg/ml, respectively. Aflatoxin B1 produced by A. flavus in concentrations of 1%, 2% and 10% Satureja essential oil were 122, 113 and 134 ppb, in 1%, 2% and 10% Mentha were 163, 168 and 171 ppb, respectively. The aqueous extracts of 1% Satureja reduced the production of toxin as 58.1 and the 1% aqueous extract of Mentha as 39.6.ConclusionThe results of this study showed that both Satureja hortensis and Mentha pulegium have the ability to inhibit the growth of Aspergillus flavus fungus, as well as control of aflatoxin B1 production in low concentrations and recommended for further studies.Keywords: Aflatoxin B1, Aqueous extract, Aspergillus flavus, Essential oil, Mentha pulegium, Satureja hortensis
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زمینه و هدفپونه از جمله گیاهانی است که در صنایع مواد غذایی به عنوان طعم دهنده مصرف فراوانی داشته و به اثرات ضد باکتریایی آن توجه شده است. مطالعه حاضر با هدف بررسی خواص فیتوشیمیایی اسانس پونه (Mentha pulegium L.) به دست آمده از ایران و فعالیت ضد میکروبی آن برعلیه تعدادی از باکتری های بیماری زا انجام شد.مواد و روش کارترکیبات شیمیایی این گیاه دارویی با GC-MS بررسی شد و 16 ترکیب مختلف که در مجموع86/96% کل وزن اسانس گیاه را تشکیل می دهند، شناسایی شدند. فعالیت ضد میکروبی اسانس گیاه پونه در برابر باکتری های استافیلوکوکوس اورئوس، اشریشیا کلی، سودوموناس آئروژینوزا و سودوموناس سیرینگه پاتوار گوجه فرنگی به روش انتشار از دیسک در مقایسه با آنتی بیوتیک سیپروفلوکساسین ارزیابی شد. حداقل غلظت مهارکنندگی اسانس در برابر باکتری های مطالعه شده با استفاده از روش ماکروبراث دایلوشن بررسی شد.یافته هانتایج به دست آمده نشان داد که اسانس پونه از اجزای اصلی پیپریتون (32/1٪)، پیپریتنون (21/71٪) و پولگون (15/85٪) تشکیل شده است. به علاوه، نتایج به دست آمده با استفاده از MICو MBCنشان داد که حداقل غلظت مهارکنندگی اسانس پونه در محدوده mg/mL 40-10 برای باکتری های استافیلوکوکوس اورئوس، اشریشیا کلی، سودوموناس آئروژینوزا و mg/mL 20 برای سودوموناس سیرینگه است. با توجه به نتایج به دست آمده، بیشترین هاله عدم رشد (mm 29/15) در سودوموناس سیرینگه و کمترین هاله عدم رشد (mm 17/08) در سودوموناس آئروژینوزا در غلظت mg/mL 40 اسانس پونه مشاهده شد.نتیجه گیرینتایج به دست آمده حاکی از این است که اسانس پونه دارای اثر مهارکنندگی مناسبی برعلیه باکتری های استافیلوکوکوس اورئوس و اشریشیا کلی می باشد.کلید واژگان: ضد باکتریایی, پونه, اسانس گیاهی, MIC, MBCBackground And AimsMentha pulegium L. is one of the medicinal plants used in the food production as a flavoring substance and its antibacterial effect has also been considered. The present study was designed to evaluate the phytochemical composition of Mentha pulegium L. originated from Iran and its antimicrobial activity against several pathogenic bacteria.Materials And MethodsThe chemical compounds of this medicinal plant were analyzed by GC-MS and 14 components were identified, representing 86.98% of its total essential mass. The antibacterial activity of the essential oil (EO) of Mentha pulegium L., has been evaluated against Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, and Pseudomonas syringae pv. tomato using disc diffusion method compared to synthetic antibiotic ciprofloxacin. The minimum inhibitory concentration (MIC) was evaluated against the above tested bacteria using macrobroth dilution method.ResultsResults showed that the EO of Mentha pulegium L., was formed by the main components of piperitone (32.1%), piperitenone (21.71%) and pulegone (15.85%). In addition, the results obtained using MIC and MBC showed that the minimum inhibitory concentration of the EO of M. pulegium was in the range of 10-40 mg/mL for S. aureus, E. coli, P. aeroginosa and 20 mg/mL for Ps. syringae pv. tomato, respectively. According to the results, the maximum zones of inhibition (29.15 mm) was observed on P. syringae P. tomato in 40 mg/mL of essential oil, and the minimum zone of inhibition (17.08 mm) was related to P. aeroginosa in the same concentration.ConclusionsThe results revealed that EO of Mentha pulegium L. had an appropriate inhibitory effect on Staphylococcus aureus and Escherichia coli.Keywords: Antibacterial activity, Mentha pulegium, Essential oil, MIC, MBC
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Background and objectives
Alternative medicine is widely used to replace a variety of commonly prescribed synthetic drugs in order to achieve a state of substantial efficacy with considerably less adverse effects. The present work has focused on the comparative evaluation of the analgesic efficacy of five members of Lamiaceae family to prioritize their potentials to be used herein.
MethodsTwo common models of pain studies including the hot-plate and tail-flick tests were used to compare the analgesic properties of Thymus vulgaris, Mentha piperita, Rosmarinus officinalis, Satureja hortensis, and Mentha pulegium essential oils (EOs) at two doses of 0.5 and 1 cc per animal.
ResultsSignificant increase in the response times of both tests were recorded compared to the control group following the administration of the EOs with the order of potency T. vulgaris 1 mL > T. vulgaris (0.5 mL) > M. piperita (1 mL) > M. piperita (0.5 mL) > R. officinalis (1 mL) > R. officinalis (0.5 mL).
ConclusionAlthough all studied EOs showed some extents of anti-nociceptive properties; however, T. vulgaris and M. piperita demonstrated the highest potential for pain management due to their rapid onset, long-lasting and steady mode of action. Their more potent anti-nociceptive effects in comparison to R. officinalis with previously proven analgesic efficacy, further supports this idea.
Keywords: Mentha piperita, Mentha pulegium, Rosmarinus officinalis, Satureja hortensis, Thymus vulgaris
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