جستجوی مقالات مرتبط با کلیدواژه "mesenchymal stem cells" در نشریات گروه "پزشکی"
-
Objective (s)
One of the leading causes of endometriosis is the return of menstrual blood flow into the pelvic cavity and the establishment of menstrual blood mesenchymal stem cells (MenSCs) outside the uterus. MenSCs from endometriosis patients (E-MenSCs) and healthy women have been shown to vary in terms of surface markers and gene expression, which may suggest the involvement of these cells in the development and expansion of ectopic lesions. This study aimed to investigate the effects of beta-catenin signaling inhibitor C-82 and naringenin as PI3K signaling pathway inhibitors on E-MenSCs to modulate their gene expression and functional pattern.
Materials and MethodsBriefly, E-MenSCs isolated by density-gradient centrifugation were treated with C-82 and naringenin, and the genes and pathways related to inflammation, proliferation, and survival were evaluated. E-MenSCs showed increased early apoptosis and decreased levels of ROS, IL-6 and IL-8, ER, α-SMA, and Ki-67 protein expression.
ResultsOur results shed light on the function of C-82 and naringenin in modulating E-MenSCs.
ConclusionHowever, more research is needed to analyze the precise effects of small molecule C-82 and naringenin on endometriosis.
Keywords: Cell Proliferation, Endometriosis, Inflammation, Mesenchymal Stem Cells, Survival -
زمینه و هدف
فعالیت منظم هوازی و درمان با سلول های بنیادی تاثیرات امیدوارکننده ای در مدیریت استئوآرتریت دارند. افزایش بیان ژن فاکتور هسته ای کاپا B (NF-κB) نقش مهمی در تخریب مفصل در شرایط استئوآرتریت ایفا می کند. همچنین، بیان ژن ماتریکس متالوپروتئیناز-1 (MMP1) در استخوان مفاصل بیماران مبتلا به استئوآرتریت افزایش می یابد. مطالعه حاضر با هدف بررسی اثرات تمرین هوازی و تزریق سلول های بنیادی بر بیان ژن های NF-κB و MMP1 در بافت غضروف زانو موش های صحرایی نر مدل استئوآرتریت انجام شد.
روش هادر این مطالعه تجربی، 25 سر موش صحرایی نر به صورت تصادفی به پنج گروه تقسیم شدند: کنترل سالم، بیمار (القای استئوآرتریت)، بیمار+تمرین، بیمار+سلول بنیادی، و بیمار+تمرین+سلول بنیادی. برنامه تمرینی شامل 30 دقیقه دویدن روی تردمیل بدون شیب با سرعت 16 متر در دقیقه در هفته اول بود که با رعایت اصل اضافه بار به صورت تدریجی به 50 دقیقه در هفته هشتم افزایش یافت. به گروه های مربوطه، سلول های بنیادی مزانشیمی با دوز 106×1 سلول بر کیلوگرم به صورت تزریق یک مرحله ای تجویز شد. 48 ساعت پس از آخرین جلسه تمرینی، بافت غضروف استخراج و بیان ژن های NF-κB و MMP1 با روشReal-Time PCR ارزیابی شد.
یافته هابیان ژنNF-κB در گروه استئوآرتریت به طور معناداری بالاتر از گروه سالم بود (P=0.001). تمرین هوازی منجر به کاهش معنادار بیان NF-κB در مقایسه با گروه بیمار شد (P=0.043). بااین حال، درمان با سلول های بنیادی به تنهایی تاثیر معناداری بر کاهش بیان این ژن نداشت (P=0.077). علاوه بر این، تفاوت معناداری در بیان ژن MMP1 بین گروه های مطالعه مشاهده نشد (P>0.05)، اما الگوی تغییرات آن مشابه تغییرات NF-κB بود.
نتیجه گیریدر حالی که درمان با سلول های بنیادی به تنهایی تاثیر معناداری بر تعدیل بیان ژن ها نشان نداد، تمرین هوازی منظم توانست بیان NF-κB را کاهش دهد. این یافته ها از پتانسیل سرکوب NF-κB از طریق تمرین ورزشی به عنوان مداخله ای موثر برای مدیریت استئوآرتریت حمایت می کند.
کلید واژگان: فاکتور هسته ای کاپا B (NF-Κb), ماتریکس متالوپروتئیناز-1 (MMP1), سلول های بنیادی مزانشیمی, استئوآرتریت, تمرین هوازیFeyz, Volume:28 Issue: 6, 2025, PP 595 -602Background and AimRegular aerobic exercise and stem cell therapy have shown promising effects in the management of osteoarthritis. The increased expression of nuclear factor kappa B (NF-κB) plays a significant role in joint degradation in osteoarthritis, while matrix metalloproteinase-1 (MMP1) gene expression is elevated in the joint bones of osteoarthritis patients. This study aimed to investigate the effects of aerobic exercise and stem cell injection on the expression of NF-κB and MMP1 genes in the knee cartilage of male rat models of osteoarthritis.
MethodsIn this experimental study, 25 male rats were randomly assigned to five groups: healthy control, osteoarthritis-induced (diseased), diseased + exercise, diseased + stem cells, and diseased + exercise + stem cells. The exercise program included 30 minutes of treadmill running at 16 meters per minute during the first week, gradually increasing to 50 minutes by the eighth week, following the principle of progressive overload. Mesenchymal stem cells were administered to the relevant groups at a dose of 1×10^6 cells per kilogram via a single injection. Forty-eight hours after the final exercise session, knee cartilage tissue was extracted, and the expression of NF-κB and MMP1 genes was evaluated using Real-Time PCR.
ResultsThe expression of NF-κB in the osteoarthritis group was significantly higher than in the healthy control group (P=0.001). Aerobic exercise resulted in a significant reduction in NF-κB expression compared to the diseased group (P=0.043). However, stem cell treatment alone did not significantly affect the reduction of this gene expression (P=0.077). Additionally, no significant differences were found in MMP1 gene expression between the study groups (P>0.05), although the pattern of changes mirrored those observed in NF-κB expression.
ConclusionWhile stem cell therapy alone did not show a significant effect on gene expression modulation, regular aerobic exercise significantly reduced NF-κB expression. These findings support the potential of aerobic exercise as an effective intervention to suppress NF-κB in the management of osteoarthritis.
Keywords: Nuclear Factor Kappa B (NF-Κb), Matrix Metalloproteinase-1 (MMP1), Mesenchymal Stem Cells, Osteoarthritis, Aerobic Exercise -
In the article published in Cell J, Vol 15, No 4, 2015, pages 364-371, the reference for Table 1 and Figures 1B-D, 2,and 5A, B was inadvertently omitted. The missing reference (24), originally written in Persian by the authors, has nowbeen added to the relevant Table and Figures' legends with the permission of the original publisher, Journal of ArdabilUniversity of Medical Sciences.The authors would like to apologies for any inconvenience caused.Keywords: Wharton’S Jelly, Mesenchymal Stem Cells, Crystallin, Differentiation
-
Objective (s)
Reducing the immune response to inflammation is vital for successful transplantation, yet chronic graft rejection remains a major issue despite immunosuppressive drugs. This study explored the effect of bone marrow mesenchymal stem cell-derived exosomes on the survival of skin allografts in mice.
Materials and MethodsC57BL/6 and BALB/c mice underwent skin allograft surgery, followed by intraperitoneal injection of exosomes, which were compared with groups receiving dexamethasone and no treatment group.
ResultsOn day 3, mild signs of graft rejection appeared in both control groups, while none were seen in the exosome-treated group. By day 14, the grafts were completely rejected in the control groups but showed mild rejection in the treatment group. Histopathology revealed severe rejection signs in the control groups, including epithelial necrosis and inflammation, while the treatment group showed signs of angiogenesis and graft acceptance. Additionally, inflammatory cytokine levels (TNF-α, IL-1β, and IL-6) were lower in the treatment group than in the positive control group, particularly on days 3 and 14.
ConclusionThe findings suggest that exosomes can prevent graft rejection and may offer a promising therapeutic approach for solid organ transplantation, though further research is needed to standardize exosome methods and evaluate cost-effectiveness.
Keywords: Exosome, Immunomodulation, Mice, Mesenchymal Stem Cells, Regeneration, Transplantation -
Background
Alzheimer's disease (AD) is a neurodegenerative disease with insidious onset and progressive destruction of behavioral and cognitive functions. Various therapies have been tested to improve or at least effectively change the course of AD. In recent years; stem cell therapy has emerged as a hopeful potential treatment for Alzheimer's. Stem cells can differentiate into various types of cells, including brain cells, potentially replacing damaged cells and improving cognitive function.
ObjectivesIn the present study we investigated the inhibitory effect of mesenchymal stem cells isolated from amniotic membrane (AM-MSCs) on neural progenitor cells (NPC) treated with Scopolamine.
MethodsNPC cell was provided by the Iranian Biological Resource Center. To expose these cells to Alzheimer's situations, scopolamine (0.05 mg/ml) treatment has been used. The inhibitory effect of mesenchymal stem cells isolated from the amniotic membrane was evaluated by using the co-cultivation method. The expression of amyloid beta (Aβ), TERM2, Tau, and ABCA7 genes, was assessed in NPC cells co-cultivated with AM-MSC by Real-time PCR. After the Co-culture of AM-MSC and NPC cells for 72 hours, we evaluated the expression of BDNF and CHAT protein in Co-cultured NPC cells by immunocytochemical test.
ResultsResults of Real-time PCR and Immunocytochemistry showed that in Co-culture of AM-MSC with NPC decreased Aß, TREM2, and Tau gene expression and increased ABCA7 expression. As well, the expression of BDNF and CHAT protein enhanced.
ConclusionsAM-MSCs have attracted much consideration. MSCs have the capability of immune regulation, regeneration, and neuroprotection These cells are a potential candidate for cell therapy due to their easy accessibility and compliance with ethical issues.
Keywords: Mesenchymal Stem Cells, Neural Progenitor Cell, TERM2, Tau, Aβ, ABCA7 -
Background
Alzheimer's disease (AD) is a neurodegenerative disease with insidious onset and progressive destruction of behavioral and cognitive functions. Various therapies have been tested to improve or at least effectively change the course of AD. In recent years; stem cell therapy has emerged as a hopeful potential treatment for Alzheimer's. Stem cells can differentiate into various types of cells, including brain cells, potentially replacing damaged cells and improving cognitive function.
ObjectivesIn the present study we investigated the inhibitory effect of mesenchymal stem cells isolated from amniotic membrane (AM-MSCs) on neural progenitor cells (NPC) treated with Scopolamine.
MethodsNPC cell was provided by the Iranian Biological Resource Center. To expose these cells to Alzheimer's situations, scopolamine (0.05 mg/ml) treatment has been used. The inhibitory effect of mesenchymal stem cells isolated from the amniotic membrane was evaluated by using the co-cultivation method. The expression of amyloid beta (Aβ), TERM2, Tau, and ABCA7 genes, was assessed in NPC cells co-cultivated with AM-MSC by Real-time PCR. After the Co-culture of AM-MSC and NPC cells for 72 hours, we evaluated the expression of BDNF and CHAT protein in Co-cultured NPC cells by immunocytochemical test.
ResultsResults of Real-time PCR and Immunocytochemistry showed that in Co-culture of AM-MSC with NPC decreased Aß, TREM2, and Tau gene expression and increased ABCA7 expression. As well, the expression of BDNF and CHAT protein enhanced.
ConclusionsAM-MSCs have attracted much consideration. MSCs have the capability of immune regulation, regeneration, and neuroprotection These cells are a potential candidate for cell therapy due to their easy accessibility and compliance with ethical issues.
Keywords: Mesenchymal Stem Cells, Neural Progenitor Cell, TERM2, Tau, Aβ, ABCA7 -
The dental pulp is essential for tooth health and sensory function. However, conventional treatments for infected or necrotic pulp, such as root canal therapy, frequently result in tooth loss and increased fracture risk. Regenerative endodontic procedures (REPs) aim to regenerate pulp tissue while preserving tooth vitality. Exosomes from human umbilical cord mesenchymal stromal/stem cells (hUCMSCs) demonstrated potential for tissue regeneration. In this case report, the potential of hUCMSC-derived exosomes for regenerating the dental pulp of a pulpectomized tooth was investigated. The patient, a 40-year-old man, was presented with irreversible pulpitis in the mandibular second premolar. The patient underwent a pulpectomy, followed by the application of a chitosan and hUCMSC-derived exosome mixture into the root canal. This project was conceived and executed as a joint project in Bushehr (Iran), Shiraz (Iran), and Aktobe (Kazakhstan) from 2022 to 2024. Exosomes were isolated and characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Clinical evaluations, including visual inspection, palpation, pulp vitality tests, radiography, and cone beam computed tomography (CBCT) imaging, were conducted over 24 weeks post-treatment. The SEM and TEM images confirmed the effective isolation and characterization of exosomes. Clinical follow-ups showed no signs of infection, swelling, or tenderness around the treated tooth. Radiographic assessments indicated periapical radiolucency and periodontal ligament widening, suggesting active healing. Despite these radiographic changes, the absence of clinical symptoms indicated successful tissue regeneration and integration. This case report demonstrated the potential of hUCMSC-derived exosomes for dental pulp regeneration, with promising results in maintaining tooth vitality and promoting healing.
Keywords: Exosomes, Regenerative Endodontics, Dental Pulp, Regeneration, Mesenchymal Stem Cells -
BackgroundUltraviolet B (UVB) irradiation induces photoaging and apoptosis in various cell types. Inhibition of UVB-induced apoptotic pathways has been explored in different apoptotic cascades. Conditioned media from human umbilical cord blood mesenchymal stem cells (CM-hUCB-MSC) contain important substances for cell regeneration. However, the potential of CM-hUCB-MSC in preventing UVB-induced apoptosis has not been clearly elucidated. Therefore, the current research was conducted to investigate the potential of CM-hUCB-MSC in inhibiting UVB-induced apoptosis and its role in the antiapoptotic signaling pathway.MethodsAn experimental in vitro study was conducted at PT. Prodia StemCell Indonesia, Jakarta, Indonesia, 2019-2022. Initially, hUCB-MSCs were isolated and cultured to produce CM-hUCB-MSC. NIH3T3 cells were pretreated with/without 50 μM LY294002, treated with/without 10% CM-hUCB-MSC, and then irradiated with/without UVB. Subsequently, the cells were analyzed using sub-G1, immunofluorescence, and immunoblotting assays. One-way analysis of variance (ANOVA) was used for data analysis, followed by Tukey’s honest significant difference (HSD) test or the Kruskal-Wallis test, followed by the Dunn-Bonferroni test using IBM SPSS Statistics software version 21. Statistical significance was determined at P<0.05.ResultsCM-hUCB-MSC significantly inhibited UVB-induced apoptosis in NIH3T3 cells (P=0.002, Dunn-Bonferroni test). CM-hUCB-MSC significantly induced Akt phosphorylation at Ser 473 in UVB-irradiated NIH3T3 cells (P<0.001, Tukey’s HSD test). The CM-hUCB-MSC-induced phosphorylation of Akt was significantly inhibited by LY294002 (P<0.001, Tukey’s HSD test).ConclusionTaken together, it can be concluded that CM-hUCB-MSC inhibits UVB-induced NIH3T3 cell apoptosis via the activation of phosphatidylinositol-3-kinase (PI3K)/Akt signaling cascades.Keywords: Ultraviolet Rays, Apoptosis, Culture Media, Conditioned, Mesenchymal Stem Cells, Proto-Oncogene Proteins C-Akt
-
Allergic asthma is a chronic inflammatory disease characterized by airway remodeling, hyperresponsiveness, and exacerbated inflammation. While most patients respond well to current treatments, a small subset remains resistant necessitating new therapeutic strategies. Due to their immunomodulatory properties, stem cells have been proposed as a promising treatment option for asthma. Stem cells can reduce airway inflammation and restore immune balance, demonstrating positive outcomes, particularly in cases of steroid-resistant asthma. However, the mechanisms underlying lung tissue repair are not clearly defined. On the other hand, there are limitations in using these cells and for clinical use of mesenchymal stem cells, which must be produced in accordance with Good Manufacturing Practice. This review article discusses the mechanisms by which stem cells may aid in asthma treatment and addresses and explores the challenges associated with their use. By addressing these areas, we can better understand the potential and limitations of stem cell therapy in asthma and develop more effective strategies to harness their therapeutic benefits for patients with uncontrolled asthma.
Keywords: Airway Inflammation, Asthma, C-Kit Cells, Mesenchymal Stem Cells, Therapeutics -
Background & Objectives
This study aimed to construct a decellularized mouse spleen scaffold and evaluate its cellular compatibility in vitro using murine bone marrow-derived mesenchymal stem cells (BM-MSCs).
Materials & MethodsA combination of physical, chemical, and enzymatic treatments was employed for mouse spleen decellularization. These included multiple freeze-thaw cycles, the ionic detergent sodium dodecyl sulfate (SDS), and enzymatic trypsin. Histological and scanning electron microscopy analyses were conducted up to 7 days post-culture to assess the impact of decellularization and cellular adaptation to the spleen scaffolds.
ResultsHistological studies revealed the attachment and penetration of BM-MSCs into the scaffolds on days 5-7 following cell seeding. Furthermore, cell migration into the scaffold was observed 5 days after the seeding process.
ConclusionThe decellularization approach utilized in this study proved to be effective and biocompatible, supporting the preservation and proliferation of BM-MSCs. These findings indicate its potential for spleen tissue engineering applications.
Keywords: Decellularization, Scaffold, Spleen Tissue, Extracellular Matrix, Mesenchymal Stem Cells, Tissue Engineering -
Background
Heart failure (HF) is a prevalent diagnosis with a significant mortality rate. Various therapeutic approaches exist for treating HF, and human adipose-derived mesenchymal stem cells-conditioned medium (hAMSCs-CM) therapy has emerged as a promising option. Despite its potential efficacy, the precise mechanism of action underlying hAMSCs-CM treatment remains unclear. To address this knowledge gap, we conducted a novel animal study to investigate the mechanism of action of hAMSCs-CM in an HF model, with a specific focus on transforming growth factor-β (TGF-β)/galectin-3, monocyte chemoattractant protein-1 (MCP1), B-type natriuretic peptide (BNP), and aldosterone (ALD).
MethodsForty adult male Wistar rats were divided into 4 groups: control, HF, culture medium, and CM. All rats, except those in the control group, received an injection of isoproterenol to induce an animal model of HF. The CM group was administered the CM, while those in the culture medium group received standard culture media. Subsequently, serum levels of fibrotic factors, including TGF-β/galectin-3, MCP1, BNP, and ALD, were measured using ELISA. Statistical analysis was performed using one-way analysis of variance and the Tukey test.
ResultsSerum levels of TGF-β/galectin-3, MCP1, BNP, and ALD were significantly elevated in the HF, CM, and culture medium groups compared with the control group (P<0.001). Additionally, these fibrotic factors were significantly reduced in the CM group compared with the HF group (P<0.001). Notably, CM therapy could not restore TGF-β/galectin-3, MCP1, BNP, or ALD levels to the normal range observed in the control group.
ConclusionOur findings indicate that hAMSCs-CM modulates the expression of inflammatory and fibrotic cytokines, such as TGF-β/galectin-3, MCP1, BNP, and ALD, in isoproterenol-induced HF in male rats. These results contribute to a better understanding of the therapeutic mechanisms underlying hAMSCs-CM treatment for HF.
Keywords: Mesenchymal Stem Cells, Heart Failure, Inflammation, Fibrosis -
Cancer, as a complicated disease, is considered to be one of the major leading causes of death globally. Although various cancer therapeutic strategies have been established, however, some issues confine the efficacies of the treatments. In recent decades researchers for finding efficient therapeutic solutions have extensively focused on the abilities of stem cells in cancer inhibition. Mesenchymal stem cells (MSCs) are multipotent stromal cells that can the most widely extracted from various sources such as the bone marrow (BM), placenta, umbilical cord (UC), menses blood, Wharton’s jelly (WJ), adipose tissue and dental pulp (DP). These cells are capable of differentiating into the osteoblasts, chondrocytes, and adipocytes. Due to the unique characteristics of MSCs such as paracrine effects, immunomodulation, tumor-tropism, and migration, they are considered promising candidates for cancer therapeutics. Currently, MSCs are an excellent living carrier for delivery of therapeutic genes and chemical agents to target tumor sites. Also, exosomes, the most important extracellular vesicle released from MSCs, act as a strong cell-free tool for cancer therapeutics. MSCs can prevent cancer progression by inhibiting several signaling pathways, such as wnt/β-catenin and PI3K/AKT/mTOR. However, there are several challenges associated with the use of MSCs and their exosomes in the field of therapy that need to be considered. This review explores the significance of MSCs in cell-based therapy, focusing on their homing properties and immunomodulatory characteristics. It also examines the potential of using MSCs as carriers for delivery of anticancer agents and their role in modulating the signal transduction pathways of cancer cells.
Keywords: Mesenchymal Stem Cells, Cancer Therapy, Cell Therapy, Exosomes, Immunomodulation, Chemotherapeutic Agents -
Lung is one of the vital organs that get severely affected by cytokine storm and sepsis, leading to the development of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). Mesenchymal stem cell- derived small extracellular vesicles (MSC- derived sEVs) are one of the therapeutic approaches for ARDS/ ALI caused by sepsis. Apart from sEVs’ ability to carry medication, MSC- derived sEVs also possess anti- inflammatory, tissue repair, and regeneration properties. Targeted drug delivery has been a crucial area of concern in the medical field, specifically for treating lung diseases. To treat lung diseases locally, inhalation of drug products has been developed. Drug delivery by inhalation has emerged as an effective method for local administration of therapeutic agents, with numerous benefits including better efficacy at lower doses and decreased toxicity. Additionally, inhalation administration is a viable option for the systemic distribution of medications due to the lungs' considerable absorption surface and their ability to bypass initial metabolism. Therefore, our hypothesis proposes the inhalation of MSC- derived sEVs as a potential strategy for alleviating acute lung injury induced by sepsis-related cytokine storm. Following the isolation and characterization of these MSC- derived sEVs, they will be administered to an animal model of sepsis via a nebulizer, either in their pure form or loaded with drugs. Several approaches will be employed to evaluate lung functionality, including histological analysis and the measurement of inflammatory and regulatory cytokine levels to assess changes in both the humoral and cellular immune systems
Keywords: Sepsis, Small Extracellular Vesicles, Inhalation, Acute Lung Injury, Mesenchymal Stem Cells -
Objective (s)
Atrophy of the muscles following denervation can lead to the death of myofibers. This study evaluated the sciatic nerve and tibialis cranialis muscle (TCM) regeneration using scaffold and cells.
Materials and MethodsNinety adult male Wistar rats were divided into six main groups (n=15) and three subgroups (2, 4, and 8 weeks). Groups: control; without surgery, Tr; sciatic nerve transected in silicone tube, S; collagen gel put inside the silicone tube, MC; placed 3×104 mast cells mixed with scaffold, MSC; placed 3×104 mesenchymal stem cells mixed with scaffold, and MC+MSC; 3×104 of each of the mast cell and mesenchymal stem cells along with scaffold. Animals were euthanized and sampled for muscle and nerve histological and nerve immunohistochemical evaluations.
ResultsDiameter of muscle fibers, ratio of the muscle fiber’s nuclei to the fibrocyte nuclei (mn/fn), ratio of the muscle fibers nuclei number to the muscle fiber’s number (mn/mf), and ratio of the blood vessels number to the number of muscle fibers (v/mf) in all treatment groups, especially the MC + MSC group, increased compared to the Tr group but the number of mast cells, the percentage of sarcoplasmolysis, and necrosis fibers decreased. Histomorphometric results of the nerve in its various parts and immunohistochemistry results also showed improved nerve conduction, especially in the MC + MSC group.
ConclusionIn this study, nerve improvement happened mainly for two reasons: cells and time. So, the most obvious improvement occurred in the group with mast and mesenchymal cells in the 8th week.
Keywords: Denervation, Histology, Mast Cells, Mesenchymal Stem Cells, Muscle, Rat, Regeneration -
International Journal of Reproductive BioMedicine، سال بیست و دوم شماره 10 (پیاپی 177، Oct 2024)، صص 821 -836مقدمه
آسیب ایسکمی/رپرفیوژن بیضه (I/R) که متعاقب پیچ خوردگی بیضه ایجاد می شود، می تواند منجر به ناباروری مردان شود.
هدفمطالعه حاضر با هدف بررسی اثر سکرتوم های سلول های بنیادی مزانشیمی مشتق از غشای آمنیوتیک انسان (hAMSCs) بر تورشن/دتورشن بیضه در موش سوری انجام شد.
مواد و روش هاکل آزمایشات در گروه آناتومی دانشگاه علوم پزشکی تهران، تهران، ایران در تاریخ اسفند ماه 1401 تا دی ماه 1402 انجام شد. 40 سر موش نر (5 تا 7 هفته، 25 تا 30 گرم) به طور تصادفی به چهار گروه زیر تقسیم شدند: 1) گروه شم: موش ها فقط جراحی ساختگی داشتند و مداخله ی دیگری دریافت نکردند. 2) گروه تورشن/دتورشن، 3) گروه کنترل منفی: تورشن/دتورشن + تزریق داخل بیضه DMEM/F-12 و 4) گروه تورشن/دتورشن + تزریق داخل بیضه فاکتورهای ترشح کننده .hAMSCs سطوح سرمی تستوسترون، رنگ آمیزی H&E و پارامترهای کیفیت اسپرم برای ارزیابی اثرات درمانی فاکتورهای ترشح شده hAMSCs بر ساختار و عملکرد بیضه استفاده شد. استرس اکسیداتیو بافتی با تعیین مالون دی آلدئید، سوپراکسید دیسموتاز، کاتالاز و گلوتاتین پراکسیداز 1 اندازه گیری شد. بیان mRNA ژن Nrf2، Keap1، Sirt1، TNF-α و P53 در بیضه از طریق واکنش زنجیره ای پلیمراز ارزیابی شدند.
نتایجنتایج نشان داد که عوامل ترشح شده از hAMSC با کاهش استرس اکسیداتیو، پاسخ التهابی و آپوپتوز از طریق تعدیل مسیر سیگنالینگ Sirt1/Nrf2/TNF-α، آسیب تورشن-دتورشن بیضه را کاهش می دهند.
نتیجه گیریفاکتورهای ترشح شده از hAMSCs باعث افزایش توان آنتی اکسیدانی، ضد التهابی و ضد آپوپتوز شد که در نتیجه باعث افزایش سطح تستوسترون، اسپرم زایی و پارامترهای کیفیت اسپرم گردید.
کلید واژگان: آسیب خونرسانی مجدد, سلول های بنیادی مزانشیمی, سکرتوم, استرس اکسیداتیوBackgroundTesticular ischemia/reperfusion injury, a significant result of testicular torsion, can lead to the risk of male infertility.
ObjectiveThe current study aimed to evaluate the effect of human amniotic membrane-derived mesenchymal stem cells (hAMSCs) secretome on testicular torsion/detorsion (T/D) in mice.
Materials and MethodsAll the experiments were performed in the Anatomy Department of Tehran University of Medical Sciences, Tehran, Iran, during the period of March 2023 to December 2023. 40 male NMRI mice (5-7 wk, 25-30 gr) were randomized into: 1) the sham group: mice received sham operations with no other interventions, 2) T/D group, 3) negative control group; torsion detorsion + intratesticular injection of Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12, and 4) the T/D group + hAMSCs secreted factors. Serum testosterone levels, hematoxylin and eosin staining, and sperm quality parameters were used to evaluate the therapeutic effects of hAMSCs secreted factors on the testicular structure and function. Tissue oxidative stress was measured by determining malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase-1. Nuclear factor erythroid 2-related factor 2, Kelch-like ECH-associated protein 1, NAD-dependent deacetylase sirtuin-1, tumor necrosis factor-alpha and tumor protein P53 mRNA expressions were assessed in testis via real-time polymerase chain reaction.
ResultsThe results showed that hAMSCs secreted factors alleviated testicular T/D injury by attenuating oxidative stress, inflammatory response, and apoptosis via modulating the sirtuin-1/ nuclear factor erythroid 2-related factor 2/tumor necrosis factor-alpha signaling pathway.
ConclusionhAMSCs secreted factors increased antioxidative, anti-inflammatory, and antiapoptotic properties which consequently increased testosterone levels, spermatogenesis, and sperm quality parameters.
Keywords: Reperfusion Injury, Mesenchymal Stem Cells, Secretome, Oxidative Stress -
Glenohumeral osteoarthritis (GOA) is characterized by chronic inflammation leading to joint damage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) are promising therapies because of their immunomodulatory functions. The anti-inflammatory effects of EVs from human Adipose-derived MSCs (hADSCs) overexpressing microRNA (miR)-146a were investigated in experimental GOA in this study.
hADSCs were transfected with a mimic negative control or miR-146a mimics. GOA was induced in C57/Bl6j mice, and subsequently, the animals were treated intra-articularly with phosphate-buffered saline, miR-146a EVs, or miR-control EVs. The expression of miR-146a and its targeted cytokines interleukin (IL)-4, IL-10, tumor necrosis factor-alpha (TNF-α), IL-17, and interferon-gamma (IFN-γ) were analyzed in the spleen of mice by enzyme-linked immunosorbent assay and in the articular cartilage by real-time polymerase chain reaction.
miR-146a EVs showed enrichment of miR-146a. In GOA mice, miR-146a EV treatment significantly reduced expression levels of inflammatory cytokines IFN-γ, IL-17, and TNF-α and increased the anti-inflammatory cytokine IL-10 and IL-4 compared to controls. miR-146a EV treatment raised the anti-inflammatory cytokines and reduced the pro-inflammatory cytokines of the spleen in treated mice.
This study demonstrates that EVs derived from hADSCs overexpressing miR-146a have enhanced anti-inflammatory potential in GOA by modulating cytokine expression and production. EVs engineered with inflammation-related miRNAs could be a cell-free therapeutic approach for GOA.Keywords: Exosome, Extracellular Vesicles, Glenohumeral, Mesenchymal Stem Cells, Osteoarthritis -
Introduction
This study aimed to assess the photobiomodulation effects of blue and red lasers on the proliferation and osteogenic differentiation of periodontal ligament mesenchymal stem cells (PDLMSCs).
MethodsPDLMSCs were cultured and tested in 4 groups. The first two groups were exposed to 445 nm diode laser irradiation (200 mW, 6 and 12 J/cm2), and the third group was exposed to
660 nm diode laser irradiation (50 mW, 4 J/cm2). The fourth group was also considered as the
control group without irradiation. Cell viability/proliferation was assessed by MTT assay. RUNX2, alkaline phosphatase (ALP), collagen type 1 (col1), and osteocalcin (OCN) were evaluated by RTPCR, and Alizarin red was used to evaluate the colonization. The data were analyzed by means of one-way analysis of variance.ResultsThe results of our study showed that cell survival/proliferation in the second group was significantly lower than that in the control group on days 1 and 7 (P<0.05). RT-PCR showed a significant increase in osteogenic genes in all three laser groups compared to the control group (P<0.05). All groups showed a significant increase in calcium content compared to the control group (P<0.05). ALP activity also confirmed the osteoblastic differentiation of cells in laser groups.
Conclusion445 nm and 660 nm lasers with the studied parameters showed positive effects on the proliferation and osteoblastic differentiation of PDLMSCs.
Keywords: Low-Level Light Therapy, Lasers, Cell Differentiation, Mesenchymal Stem Cells -
Introduction
The adhesion ability of mesenchymal stem cells can significantly affect their viability and is considered a prerequisite for cell therapy. The current study sought to evaluate the effect of fibroblast growth factor 2 (FGF2) and low-level laser therapy (LLLT), either individually or in conjunction, on the adhesion and proliferation of periodontal ligament stem cells (PDLSCs) when applied on the first day of cell seeding.
MethodsThe experimental groups of this study comprised a control group and different combinations of adjunctive FGF2 (50 ng/mL) and LLLT with an 808 nm diode laser in one (LLLT1) or two sessions (LLLT-2) of irradiation. The proliferation and adhesion of cells were evaluated by using the methylthiazolyl tetrazolium (MTT) assay and 4′,6-diamidino-2-phenylindole (DAPI) staining. All experiments were done in triplicates on the first, third, and fifth days after cell seeding. Two-way ANOVA and post hoc Tukey tests were used to analyze the data of the MTT assay. P<0.05 was considered statistically significant.
ResultsOne-day post-culture, only significant differences were found between the control group and the FGF2 (P=0.04) and FGF2+LLLT-2 application (P=0.04) groups. After three days post-cell culture, only a significantly higher proliferation rate was found in the control group than in the FGF2 group (P=0.01). After five days, the control group and LLLT-2 groups showed significantly higher amounts of proliferation compared to the other groups (P<0.05). DAPI staining qualitatively confirmed the results of the MTT assay.
ConclusionThe LLLT can be applied to PDLSCs on the day of seeding without causing a notable decrease in their viability and adhesion. Conversely, the administration of FGF2 should be restricted on the seeding day and postponed to subsequent days as it may have adverse effects on their adhesion and proliferation.
Keywords: Cell Adhesion, Cell Proliferation, Fibroblast Growth Factor 2, Lasers, Semiconductor, Mesenchymal Stem Cells -
Introduction
Exosomes function as cell signaling carriers and have drawn much attention to the cell-free treatments of regenerative medicine. This meta-analysis aimed to investigate the efficacy of mesenchymal stem cell-derived (MSCderived) exosomes in animal models of spinal cord injuries (SCI).
MethodsA comprehensive search was conducted in Medline, Embase, Scopus, and Web of Science to attain related articles published by January 31, 2023. The eligible keywords were correlated with the spinal cord injury and MSC-derived exosomes. The evaluated outcomes were locomotion, cavity size, cell apoptosis, inflammation, neuro-regeneration, and microglia activation. A standardized mean difference was calculated for each sample and a pooled effect size was reported.
Results65 papers fully met the inclusion criteria. TreatmentwithMSC-derived exosomes ultimately improved locomotion and shrunk cavity size (p<0.0001). The administration ofMSC-derived exosomes enhanced the expression of beta-tubulin III, NF200, and GAP-43, and increased the number of NeuN-positive and Nissl-positive cells, while reducing the expression of glial fibrillary acidic protein (p<0.0001). The number of apoptotic cells in the treatment group decreased significantly (p<0.0001). Regarding the markers of microglia activation, MSC-derived exosomes increased the number of CD206- and CD68-positive cells (p=0.032 and p<0.0001, respectively). Additionally, MSC-derived exosome administration significantly increased the expression of the anti-inflammatory interleukin (IL)-10 and IL-4 (p<0.001 and p=0.001, respectively) and decreased the expression of the inflammatory IL-1b, IL-6, and TNF-a (p<0.0001).
ConclusionsMSC-derived exosome treatment resulted in a significantly improved locomotion of SCI animals through ameliorating neuroinflammation, reducing apoptosis, and inducing neuronal regrowth by facilitating a desirable microenvironment.
Keywords: Exosomes, Mesenchymal Stem Cells, Spinal Cord Injury -
Background
There have not been any clear studies on the use of mesenchymal stem cells (MSCs) to treat osteoarthritis (OA) in the knee.
Materials and MethodsThis study investigates the effects of different MSC dosages on pain alleviation in individuals with OA in the knee by conducting a meta?analysis of existing randomized controlled trials. Electronic resources such as Google Scholar,PubMed, Cochrane Library, and Web of Science were searched up until June 2023. Treatment effect sizes were computed using the knee osteoarthritis outcome score (KOOS), the Western Ontario and McMaster Universities Osteoarthritis Index WOMAC), and the Knee Society Score (KSS). Random or fixed effect models were applied to aggregate the data. We performed a subgroup analysis according to dosage level. The heterogeneity of the research was investigated using the Chi?square test and the I2 index.
ResultsThe meta?analysis included 26 studies with a total sample size of 739 patients. A significant reduction in pain was observed 1 year and 2 years following the injection of MSCs into the injured joint, as indicated by the Visual Analogue Scale, WOMAC, KOOS, and KSS indexes (P < 0.05). Patients on MSCs reported much reduced pain after 1 and 2 years compared to the control group (P < 0.05). Subgroup and meta?regression analyses revealed no statistically significant variations in the effectiveness of MSC dosage (P < 0.05). The studies did not report any adverse effects.
ConclusionDifferent dosages of MSCs had the same pain?relieving effectson patients with OA in the knee. MSC injections were safe and beneficial in such cases.
Keywords: Knee, Mesenchymal Stem Cells, Meta‑Analysis, Osteoarthritis, Safety, Treatment Outcome
- نتایج بر اساس تاریخ انتشار مرتب شدهاند.
- کلیدواژه مورد نظر شما تنها در فیلد کلیدواژگان مقالات جستجو شدهاست. به منظور حذف نتایج غیر مرتبط، جستجو تنها در مقالات مجلاتی انجام شده که با مجله ماخذ هم موضوع هستند.
- در صورتی که میخواهید جستجو را در همه موضوعات و با شرایط دیگر تکرار کنید به صفحه جستجوی پیشرفته مجلات مراجعه کنید.