جستجوی مقالات مرتبط با کلیدواژه "platelet derived growth factor" در نشریات گروه "پزشکی"

 Applying autologous growth factors and diode laser in periodontal therapy enhances fibroblast-mediated new attachment and osteoblastic differentiation. Hence, this study compared and evaluated the effectiveness of concentrated growth factor (CGF) alone and with diode laser application in managing intrabony periodontal defects.

 Ten patients with stage III periodontitis were included in this study. All the patients underwent an open flap debridement (OFD) procedure followed by CGF membrane placement in the intrabony defect in site A, whereas, in site B, after OFD, all the patients underwent diode laser irradiation before CGF membrane placement. Plaque and gingival bleeding index (PI & GBI), PPD, and clinical attachment level (CAL) were evaluated at baseline and 3 and 6 months later. Bone fill (BF), BF%, bone crest changes (BCC), and BCC% were assessed radiographically at six months postoperatively.

 Significant reductions in PI and GBI scores, probing pocket depth (PPD), and CAL gain were observed at both sites 3 and 6 months from baseline. A significant reduction in PPD and CAL gain was noted between sites, which were higher in site B than in site A with a mean difference of 0.70±0.05 mm and 1.30±0.18 mm, 0.90±1.89 mm at 3 and 6 months, respectively. Radiographic measurement showed better BF, BF%, BCC, and BCC% at both sites at six months, which were higher at site B than at site A but statistically insignificant.

 The combination of CGF and diode laser application has demonstrated successful and promising results in terms of regeneration, improving the clinical and radiographic parameters.

Umbilical cord blood hematopoietic stem cells (UCB-HSCs) are an attractive source for transplantation. The generation of megakaryocyte-committed cells could lead to shorten period of thrombocytopenia after HSCs transplantation. Platelet lysate (PL) unlike fetal bovine serum (FBS) can prevent immune problems as well as avert transmission of certain diseases to the recipient. In this study, the authors aimed to assess the effect of PL on UCB CD34+ cells expansion and megakaryocyte differentiation.

In this experimental study, PL prepared and the subsequent isolation of UCB CD34+ cells were done by magnetic cell sorting. The isolated cells were cultivated in Iscove’s Modified Dulbecco’s medium (IMDM) supplemented with PL or FBS. Cell expansion was evaluated using Trypan blue. Furthermore, Flow cytometry using monoclonal antibodies (CD41-FITC and CD42b-PE) and the expression of specific genes including GATA1, GATA2, FLI1, NFE2, and RUNX1 via real-time PCR were performed to evaluate the megakaryocyte differentiation.

The results showed that PL insignificantly enhanced UCB CD34+ cell expansion (32.83± 8.47 fold in FBS and 41.67± 10.31 fold in PL containing media). Besides, flow cytometry results showed that expression of CD41 was increased markedly (37.81± 4.78 fold in FBS and 45.78 ± 7.37 in PL containing media, P-value <0.05) but the elevation of CD42b (10.53 ± 2.13 and 13.20 ± 2.06 in FBS and PL containing media, respectively) was not significant (P-value = 0.051). The results of real-time PCR demonstrated a notable increase in GATA binding protein 1 (1.58, P-value <0.01), GATA binding protein 2 (2.45, P-value <0.001), RUNX family transcription factor 1 (1.60, P-value <0.01), Fli-1 proto-oncogene (1.87, P-value <0.001) in PL supplemented media, however, the increase of Nuclear Factor-Erythroid 2 gene expression was not significant in PL supplemented media (P-value = 0.11).

PL improved UCB CD34+ cells expansion and megakaryocyte differentiation compared to FBS.

Angiogenesis in adipose tissue plays an important role in the development or reduction of this tissue. Since exercise is essential in preventing obesity, the purpose of the present study was to investigate the effects of high intensity interval training (HIIT) and moderate intensity continues training (MICT) on platelet derived growth factor (PDGF) gene expression in visceral and subcutaneous adipose tissues in male Wistar rats.

In this experimental study, 24 male Wistar rats with 250-350 gram body masses were selected. As the study sample, the rats were divided into four groups: (1) basal control, (2) 8 weeks control, (3) MICT and (4) HIIT. Training program consisted of 8 weeks and 5 days per week running of MICT (15 to 30 m/min for 15 to 60 minutes) or HIIT (4 to 8 single-minute intervals of intense activity at a speed of 28-55 m/min and one-minute intervals of mild activity at a speed of 12-30 m/min). Adipose tissue samples were removed 48 hours after the last training session and PDGF gene expression was measured by Real-Time PCR methods. Kruskal-Wallis, one way ANOVA and Tukey post hoc tests were used to analyze the results at the significance level of 0.05.

Results indicated that rats’ weight of control group significantly increased (P= 0.001), but in HIIT (P = 0.34) and MICT (P = 0.22) groups, physical training prevented weight gain. In addition, there were significant effects neither subcutaneous (P = 0.38) nor visceral (P = 0.38) adipose tissues by HIIT and MICT on PDGF gene expression. However, both types of exercise activities, especially the HIIT exercise, increased the PDGF gene expression about 2.5 to 3 times in both adipose tissues.

It seems that the HIIT and MICT for 8 weeks do not have significant effects on PDGF gene expression in subcutaneous and visceral adipose tissues, although further studies are needed to clarify the issue.

The anticancer agent imatinib (IM) is a small molecular analog of ATP that inhibits tyrosine kinase activity of platelet derived growth factors (PDGFs) and stem cell factor (SCF) receptor in cancer cells. However these factors have a key role in regulating growth and development of normal Sertoli, Leydig and germ cells.

The aim of this study was to determine cell viability, PDGF and SCF levels in mouse normal Sertoli cells exposed to IM.

In this experimental study, the mouse TM4 Sertoli cells were treated with 0, 2.5, 5, 10 and 20 μM IM for 2, 4 or 6 days. The cell viability and growth factors levels were assessed by MTT and ELISA methods, respectively. For statistical analysis, One-Way ANOVA was performed.

IM showed significant decrease in Sertoli cell viability compared to control group (p=0.001). However, IM increased PDGF and SCF level insignificantly (p>0.05).

Results suggested that IM treatment induced a dose dependent reduction of cell viability in Sertoli cells. It seems that treatment with this anticancer drug is involved in the fertility process. Further studies are needed to evaluate the role of PDGF and SCF in this cell.