جستجوی مقالات مرتبط با کلیدواژه « random skin flap » در نشریات گروه « پزشکی »
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BACKGROUND
Randomized skin flaps have been used as a basic treatment modality for covering skin defects for a long time but they have always been in the risk of an inherent ischemia. Fibroblast growth factor 1 is a known angiogenic factor in in vitro studies which has shown conflicting results in in vivo investigation. We aimed to determine the effect of recombinant fibroblast growth factor on the angiogenesis rate of random cutaneous flap in animal model of rats.
METHODSThis experimental study was conducted on 24 adult male rats randomized to 2 groups. In the first group FGF1 was injected subdermally in equally divided doses and distances of random flap surface in days 1, 3 and 5. In second group, normal saline was injected as control. Flap surgery was done on day 21 after first injection. The extent of necrosis and angiogenesis (mean vessel density) were assessed in day 14 after surgery.
ResultsThe mean percentage of clinically apparent necrosis was 35.2% (±10.5) in intervention (FGF1) group and 38.1% (±8.7) in control (normal saline), re-spectively. Mean vessel density was 86.20±5.6/mm2 in control group and 90.17±5.5/mm2 in intervention group, which showed no statistically signifi-cant difference.
CONCLUSIONMean vessel density and mean percentage of clinically apparent necrosis area were similar in 2 groups of rats with random cutaneous flaps receiving FGF1 or normal saline.
Keywords: Fibroblast growth factor 1, Angiogenesis, Random skin flap} -
Journal of Advances in Medical and Biomedical Research, Volume:26 Issue: 119, Nov Dec 2018, PP 24 -33Background & ObjectiveWound dressing and healing in diabetic patients is encountered with many problems. This study aims to investigate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the survival of random skin flap (RSF) on Streptozotocin-induced diabetic rats (STZ) using an optical microscope.Materials & MethodsIn this study, 60 male Albino Wistar rats were used (average weight 250-300 gr). The rats were divided into six groups: 1). Health-Non (HN), 2). Health- Cells (HC), 3). Health –Sham (HS), 4). Diabetic-Non (DN) that were became diabetic by injecting STZ 70 mg/kg intraperitoneally), 5). Diabetic-Sham (DS), 6). And Diabetic-Cell (DC). In all groups, the day of surgery was considered as the zero day, on the back area of animal, the flap was created with a size of 8 × 3 cm and the BM-MSCs were performed. The sampling was performed on day 7 after surgery from the region where Transitional Zone (TZ) necrosis was initiated.ResultsBM-MSCs increased the number of blood vessels (P=0.009) and the histology parameters (wound demarcation P=0.0001, granulation tissue P=0.0001) significantly compared to the control group. But this increase was not significant in the area of the survival region.ConclusionIt was concluded that after treatment with BM-MSCs, the wound healing process in both non-diabetic and diabetic groups was increased in accordance with histological characteristics.Keywords: Diabetes mellitus, Random skin flap, Bone marrow, Stem cells, Streptozotocin, Survival}
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BackgroundIschemia of skin flaps is an important complication in reconstructive surgery. This study evaluated the effectiveness of eutectic mixture of lidocaine and prilocaine (EMLA) and capsaicin on improving flap survival.MethodsA number of 39 white albino male rats were divided randomly into three groups: EMLA, capsaicin, and control groups. A standard rectangular, distally based dorsal random pattern skin flap was elevated on each rat. Intraperitoneal cefazolin was administered to prevent infection. No pharmaceutical agent was administered for the control group except for pure Vaseline cream. In EMLA group, EMLA cream was administrated daily after surgery for 10 days. In capsaicin group, capsaicin cream was rubbed on the flap surface daily. The rats were evaluated 10 days after the operation for viable and necrotic portions of flaps.ResultsThe mean values of necrosis in the flaps were 603.33 ± 116 mm2, 665.00 ± 220.26 mm2, and 920.00 ± 247.31 mm2 in the EMLA, capsaicin, and control groups, respectively. Both EMLA and capsaicin were effective on flap survival significantly (P = 0.002 and P = 0.011, respectively). Despite advantages, EMLA was not significantly better than capsaicin (P = 0.739).ConclusionsEMLA and capsaicin are effective pharmaceutical agents that significantly increase the viability of random skin flaps in rats. They can be added to other vasoactive topical agents to reach better results and be used as a medical workhorse in reconstructive wards.Keywords: Eutectic mixture of lidocaine, prilocaine, Capsaicin, Random skin flap, Flap}
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Background
Ischemia of skin flaps is an important complication in reconstructive surgery. This study evaluated the effectiveness of eutectic mixture of lidocaine and prilocaine (EMLA) and capsaicin on improving flap survival.
MethodsA number of 39 white-albino male rats were divided randomly into three groups: EMLA, capsaicin, and control groups. A standard rectangular, distally based dorsal random pattern skin flap was elevated on each rat. Intraperitoneal cefazolin was administered to prevent infection. No pharmaceutical agent was administered for the control group except for pure Vaseline cream. In EMLA group, EMLA cream was administrated daily after surgery for 10 days. In capsaicin group, capsaicin cream was rubbed on the flap surface daily. The rats were evaluated 10 days after the operation for viable and necrotic portions of flaps.
ResultsThe mean values of necrosis in the flaps were 603.33 ± 116.00 mm2, 665.00 ± 220.26 mm2, and 920.00 ± 247.31 mm2 in the EMLA, capsaicin and control groups, respectively. Both EMLA and capsaicin were effective on flap survival significantly (P = 0.002 and P = 0.011, respectively). Despite advantages, EMLA was not significantly better than capsaicin (P = 0.739).
ConclusionsEMLA and capsaicin are effective pharmaceutical agents that significantly increase the viability of random skin flaps in rats. They can be added to other vasoactive topical agents to reach better results and be used as a medical workhorse in reconstructive wards.
Keywords: Eutectic mixture of lidocaine, prilocaine, Capsaicin, Random skin flap, Flap survival} -
Objective(s)The aim of this study is to determine the effects of progesterone treatment on the survival of random skin flaps.Materials And MethodsMcFarlane flaps were established and 40 male rats were randomly assigned to the progesterone-treated as the test group or normal saline-treated as the control group. Progesterone or normal saline (10 mg/kg) was administered intraperitoneally once daily. On postoperative day 2, malondialdehyde (MDA) and superoxide dismutase (SOD) were detected using test kits. Flap survival rates were evaluated with transparent graph paper under direct visualization, the levels of inflammation were examined by haematoxylin and eosin (H&E) staining, and the expression of vascular endothelial growth factor (VEGF) was immunohistochemically evaluated on day 7.ResultsCompared to that in the control group, the mean survival area was significantly larger in the progesterone group. SOD activity was increased significantly, but the MDA levels in the test group were decreased. H&E-stained slices revealed that inflammation was inhibited in the test group. VEGF expression markedly increased in the progesterone group.ConclusionThis study showed that progesterone administered intraperitoneally significantly improved random skin flap survival in rats.Keywords: Angiogenesis, Malondialdehyde, Progesterone, Random skin flap, Superoxide dismutase, Vascular endothelial growth factor}
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زمینه و هدفبازسازی پوست با بافت همنوع خود در جراحی پلاستیک و ترمیمی اهمیت ویژه ای دارد و فلپ پوستی با الگوی راندوم یکی از شایعترین اعمال جراحی در جراحی پلاستیک و ترمیمی می باشد. نکروز شدن پوست از عوارض غیرقابل اجتناب بعد از جراحی فلپ است که عوامل متعددی از قبیل ایسکمی، جریان خون ناکافی و تجمع وریدی در ایجاد نکروز نقش دارند. داروهای زیادی برای جلوگیری از این عارضه توصیه شده اند. هدف ما در این مطالعه بررسی تاثیر داروی آنژی پارس را بر افزایش خونرسانی فلپ در مدل حیوانی موش می باشد.مواد و روش هابیست موش صحرایی (رت) نر از نژاد Sprague-Dawley با وزن تقریبی 300-350 گرم انتخاب شده و به طور تصادفی به دو گروه تقسیم شدند. در تمام موش ها پس از بیهوشی یک فلپ به ابعاد 2×7 در خلف ایجاد شد. کرم آنژی پارس به طور موضعی به میزان 0/5 سی سی روزانه در محل فلپ ایجاد شده استفاده شد. پس از ده روز تحت اندازه گیری و محاسبات قرار گرفت. از میانگین و انحراف معیار برای توصیف داده ها و از آزمون تی تست مستقل جهت مقایسه گروه ها استفاده شد.یافته هادر طی مطالعه یک مورد از گروه کنترل را از دست دادیم. هیچ موردی از عفونت یا هماتوم مشاهده نشد. در پایان محاسبات میزان نکروز فلپ در گروه آنژیوپارس 6/43 ±28/86درصد و میزان نکروز فلپ در گروه کنترل 10/52±25/03 درصد بود. تفاوت دو گروه با P-value کمتر از 05/0 معنی دار نبود.نتیجه گیریدر این مطالعه می توان گفت کرم آنژیوپارس در افزایش میزان خونرسانی فلپ راندوم در موش تاثیرگذار نبوده است.
کلید واژگان: فلپ پوستی راندوم, نکروز, آنژی پارس}Introduction &ObjectiveRegeneration of skin tissue is important in the plastic and reconstructive surgery and random skin flap pattern is one of the most common procedures in plastic and reconstructive surgery. Necrosis of the skin flap is an inevitable complication after the surgery. Several factors such as ischemia, insufficient blood flow and venous concentration are causes in necrosis creation. Pharmacological agents and modifications of procedures have been proposed to avoid this complication. In this study, the effects of Angipars cream on skin random flap survival were examined in rats.Materials and MethodsTwenty male Sprague – Dawley rats were selected and randomly assigned to two equal groups. Surgery was done under general anesthesia and a rectangular 2×7 cm flap was generated on the back of each rat and it was sutured once again. Angipars cream was used on flap pedicel in control group and Vaseline was used in the other group for ten days. The mean and standard deviation was used to assess the test data and independent t-test was used to compare the groups.ResultsDuring the study, one rat of the control group died. There were no cases of infection or hematoma. The necrosis rate of the angipars group was 28.86 ± 6.43 percent and in the control group was 25.3 ± 10.52 percent. There was no significant difference in necrosis rate between the two groups.ConclusionsAngipars cream has no effect on increasing perfusion of random flap. Furthermore, also increased angiogenesis was not observed in this study.Keywords: Random Skin Flap, Necrosis, Angipars} -
زمینه و هدف
فلپ تصادفی در جراحی ترمیمی کاربرد زیادی دارد و نکروز آن از مشکلات جراحی پلاستیک است. داروهای سیستمیک و موضعی زیادی برای جلوگیری از این عارضه توصیه می شود. در این مطالعه اثر آسپیرین و پلاویکس و هم چنین اثر ترکیب دو دارو بر بقای فلپ پوستی تصادفی در رت بررسی شد.
روش بررسی40 رت به طور تصادفی به چهار گروه ده تایی تقسیم شدند. پس از بیهوشی عمومی فلپ های cm3×11 در پشت تمام رت ها ایجاد شد. سپس در یک گروه روزانه mg/kg100 آسپیرین خوراکی به مدت یک هفته و در گروه دیگر پلاویکس روزانه mg/kg25 خوراکی به مدت یک هفته تجویز شد. در گروه سوم آسپیرین به علاوه پلاویکس با دوز مشابه گروه اول و دوم به مدت یک هفته استفاده شد. حیوانات گروه چهارم به عنوان گروه کنترل بوده و دارویی دریافت نکردند. پس از هفت روز سطح فلپ تحت اندازه گیری و محاسبات مورفولوژی قرار گرفت. از میانگین و انحراف معیار جهت توصیف داده ها و از آزمون آنالیز واریانس جهت مقایسه گروه ها استفاده شد.
یافته هامیانگین میزان زنده بودن فلپ پوستی تصادفی (نسبت سطح زنده فلپ به سطح کل×100) در گروه کنترل 49/62%، در گروه آسپیرین 04/64%، در گروه پلاویکس 09/65% و در گروه ترکیبی 06/64% بود. مقایسه اختلاف میانگین بین گروه ها نشان داد که اختلاف معنی داری بین گروه های مطالعه با گروه کنترل وجود نداشت (05/0<P).
نتیجه گیریمصرف آسپیرین، پلاویکس و ترکیب آن ها تاثیر قابل توجهی بر بقای فلپ پوستی تصادفی نداشت
کلید واژگان: فلپ, فلپ پوستی تصادفی, ایسکمی, نکروز فلپ, آسپیرین, پلاویکس}BackgroundRandom pattern flap is a common reconstructive surgery procedure but its necrosis is a challenging problem. A lot of pharmacological agents and surgical procedures have been examined for the prevention of this complication to maximize the length to width ratio of these surgical flaps. Therefore, we designed an experimental study to evaluate the effects of aspirin, clopidogrel bisulfate (Plavix) and their combination on random skin flap survival in rats.
MethodsForty male rats were randomly assigned to four equal groups. Surgery was done under general anesthesia. A random, rectangular 3×11 cm dorsal skin flap was designed, elevated and sutured back into its primary site. In group one, 100 mg/kg Aspirin and in group two, 25 mg/kg Plavix were administered orally for 7 days postoperatively. Aspirin and Plavix were co-administered in the third group for the same period of time while the control group received no medication. After 7 days, the total surface of flaps, the viable and also the necrotic parts were measured by Image J software. Mean standard deviation and analysis of variance were calculated to compare the results.
ResultsThe mean area of flap survival was 62.49% in the control, 64.04% in Aspirin, 65.09% in Plavix and 64.06% in combination groups. No statistically significant differences were found between treatment groups and control rats.
ConclusionIn this study, we found no significant differences between Aspirin, Plavix or their combination on the survival of random skin flaps.
Keywords: aspirin, clopidogrel, ischemia, necrosis, random skin flap}
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