جستجوی مقالات مرتبط با کلیدواژه "remineralization" در نشریات گروه "پزشکی"

 This in vitro study compared the remineralization potential of Dabur red (DR), a non-fluoridated herbal toothpaste, with Colgate Strong Teeth (CST), a fluoride-based toothpaste, on permanent teeth with artificially created white spot lesions. Vickers microhardness (VMH), quantitative light-induced fluorescence system (QLF), and scanning electron microscopy (SEM) were used for data analysis.

 Enamel samples were prepared from the buccal surface of forty-five extracted maxillary premolars and subjected to demineralization to achieve adequate fluorescence loss (ΔF). The samples were divided into DR, CST, and control groups (n=15) and underwent 21 days of pH cycling. VMH was measured at baseline and after 21 days. QLF imaging was performed on days 7, 14, and 21. One randomly selected sample from each group underwent SEM examination to compare morphological variations. The statistical analysis was conducted using SPSS 22.0. Intra-group comparisons were made with repeated measures of analysis of variance (ANOVA), and one-way ANOVA with Tukey’s post-hoc test was utilized for inter-group comparisons (P<0.05).

 Mean VHN increased significantly in all three groups from baseline (DR=40.47, CST=41.91, control=36.42) to the 21st day (DR=119.47, CST=120.36, control=74.36) (P=0.00). Significant differences were found between the control and test groups, but not between DR and CST. No statistically significant difference was observed in ΔF at baseline and 7 days, but there were significant differences among the groups at 14 and 21 days. SEM images revealed dense mineralization for both DR and CST.

 After 21 days of in vitro remineralization, both DR and Colgate toothpaste demonstrated similar remineralizing potential as evaluated by surface MH and QLF.

 This study evaluated the efficacy of grape seed extract (GSE) on the remineralization of primary tooth enamel alone or in combination with remineralizing agents.

 The initial microhardness value of 90 primary tooth enamel samples was calculated; then, the samples were demineralized. The post-demineralization hardness of the samples was measured and the samples were randomly divided into 6 groups as follows: G1: negative control, G2: GSE, G3: NaF, G4:Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), G5: GSE+NaF, and G6: GSE+CPP-ACP (n=15). Oral environment pH cycle was applied and hardness measurements were repeated after treatments. The samples were stained with 1% rhodamine B dye and sectioned, and the lesion depth was measured. Statistical significance was set at P<0.05.

 The hardness decrease of the GSE and GSE+NaF groups was less than the other groups (P<0.05). The decrease was also less in the other groups than in the control group (P>0.05). GSE showed a positive effect when combined with NaF in maintaining microhardness but did not show the same effect when combined with CPP-ACP (P<0.05). Concerning penetration depth, all the groups had statistically lower values than the control group (P<0.05). The lowest penetration rates were observed in the GSE+NaF and GSE+CPP-ACP groups (P<0.05).

 The lowest hardness decrease was observed in the GSE and GSE+NaF groups, and the lowest penetration rates were observed in the GSE+NaF and GSE+CPP-ACP groups. It has been determined that a 15% GSE solution might be used as an alternative to fluoride in primary tooth remineralization and can increase the effectiveness of fluoride when used together.

The acidic component of liquid medicinal syrups used by pediatric patients may cause erosion and partial demineralization. This study aimed to evaluate the effect of cheese and casein phosphopeptide‑amorphous calcium phosphate (CPP‑ACP) on erosive lesions of primary teeth enamel following exposure to amoxicillin and ibuprofen syrups.

In this in vitro study, 60 noncarious deciduous molars were used. After measuring the surface microhardness of the samples, they were randomly separated into two groups and immersed in either amoxicillin or ibuprofen for 1 min three times per day. CPP‑ACP, cheese, and artificial saliva were then applied to each of the three subgroups (n = 10). After each immersion time, 10 min of therapy was given. Between treatment intervals, the samples were kept in artificial saliva. The microhardness was remeasured after 1 week. Data were analyzed using SPSS software through repeated‑measures ANOVA (α = 0.05).

All samples’ microhardness reduced considerably after immersion in liquid pharmaceuticals(amoxicillin [84.9 kgf/mm2 ] and ibuprofen [75.1 kgf/mm2 ]), but increased significantly following exposure to therapeutic solutions. There was no difference between the amoxicillin‑cheese and amoxicillin‑CPP‑ACP subgroups (P = 0.975). A statistically insignificant difference was found between the ibuprofen group and the ibuprofen‑CPP‑ACP subgroup (P = 0.499).

As a result, cheese and CPP‑ACP can be utilized to remineralize erosive lesions caused by amoxicillin or ibuprofen exposure.

The purpose of this study was to investigate the effect of adding CPP-ACP into a daily-use toothpaste on the remineralization of enamel caries lesions. Thirty enamel blocks were obtained from bovine incisors. Each specimen was divided into three equal parts. One-third of each block was coated with varnish to serve as a sound control area, while the remaining two-thirds underwent a demineralization process. After demineralization, another one-third of the surface was varnished, leaving only one-third of the enamel to undergo remineralization. The enamel blocks were divided into three groups (n=10), according to the remineralization treatment applied as follows: Group 1: fluoride-containing toothpaste, Group 2: CPP-ACP-containing toothpaste, and Group 3: fluoride- and CPP–ACP–containing toothpaste.  Remineralization was assessed through the Vickers microhardness test at various depths (20, 50, 120 and 200 µm). The data were analyzed by ANOVA and LSD test, and P< 0.05 was considered statistically significant. There was a significant difference in remineralization efficacy between the groups at the depth of 20 µm (P 0.001). Pairwise comparisons revealed that the toothpaste containing both fluoride and CPP-ACP had a significantly greater microhardness than other experimental groups (P 0.05). No significant difference was observed between the study groups concerning microhardness at 50, 120 and 200 µm depths (P 0.05). CPP-ACP can serve as a suitable alternative to fluoride in daily-use toothpaste for enamel remineralization. The concurrent use of fluoride and CPP-ACP in toothpaste can generate a synergistic remineralizing effect at the enamel surface layer.

The present study aimed to use a DIAGNOdetnt unit to compare the effects of casein phosphopeptideamorphous calcium phosphate fluoride (CPP-ACPF), 0.05% sodium fluoride (NaF) mouth rinse, and glass ionomer (GI) on enamel remineralization.

This in vitro study was performed on 50 intact human premolars in five groups: A (artificial saliva), B (CPP-ACPF), C (NAF), D (GI), and E(GI+NAF). All samples were assessed using a DIGNOdetnt unit before and after demineralization. The teeth in each group were treated (except for the control group) then pH cycled repeatedly for 30 days. After this, the remineralization evaluation was evaluated. Data gathered by DIAGNOdetnt were analyzed by One way analysis of variance. The least significant difference (LSD) test was used for multiple comparisons (α=0.05).

After the treatment, the mean DIAGNOdetnt values in groups B(3.6), C(4.7), D (5.1), and E(3.5) were significantly lower than that in group A(P<0.0001). LSD test showed that the mean DIAGNOdetnt value in groups B and E was lower than for groups C and D(P<0.001).

Although the repeated application of all the experimental materials was efficient, CPP-ACPF and GI+NAF had more prominent remineralization potential in comparison to NAF and GI alone.

The purpose of this in vitro study is to ascertain how NovaMin and Er,Cr:YSGG laser radiation affect the remineralization of primary tooth enamel lesions.

40 main teeth served as the study’s sample size. These teeth were allocated into five groups at random. The first group served as a negative control (artificial saliva); the second group served as a positive control (APF gel 1.23%); the third group NovaMin, the fourth group Er,Cr:YSGGlaser, the fifth group Er,Cr:YSGG laser and finally, the application of NovaMin. For the purpose of measuring microhardness, all samples were submitted to the dental materials laboratory three times.

The collected data were compared using the SPSS 28 program between the baseline measurement, after demineralization, and after remineralization. The data were analyzed using ANCOVA and Bonferroni tests. All groups demonstrated a considerable increase in microhardness as compared to the negative control group (P < 0.05). With the exception of the third and fourth groups, there was no discernible difference in the rise in microhardness between the other groups. The third and fourth groups were compared, and the results revealed that NovaMin had a greater impact than laser therapy alone (P = 0.023). Scanning electron microscopy (SEM) analysis backed up the findings.

In comparison to using the Er,Cr:YSGG laser alone, using NovaMin alone can speed up the remineralization of erosion lesions on the surface of primary teeth.

This study evaluated the color changes induced by the treatment of intact and demineralized enamel surfaces with casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and sodium fluoride.

Ninety-six sound teeth were collected. The samples were divided into “intact” and “demineralized” groups and then subjected to different treatments (CPP-ACFP, sodium fluoride, and CPP-ACP). After the treatment phase, the colors of samples were evaluated and compared with the pretreatment values.

Both demineralized and intact enamel samples underwent increased whiteness (L*) and reduced yellowness (b*) upon treatment with any of the agents (P < 0.001). More pronounced results were achieved with CPP-ACP.

These treatments generally make the teeth look whiter, brighter, and clearer with a more natural appearance.

Formation of white spot lesions (WSL) subsequent to orthodontic treatment is a cosmetic concern. This research assessed the effects of bioactive-glass toothpaste on remineralization of orthodontic white spot lesions.

Orthodontic brackets were boned to seventy extracted premolars. Then they were submersed in a demineralization solution (pH=4.52) at 37°C for 96 hours, which led to the formation of artificial caries lesions on enamel. Occlusal brackets were assigned to the treatment group, whereas gingival brackets were assigned to the control group.Specimens were allocated to one of two groups: group 1) toothpaste containing sodium fluoride (1,450 ppm), group 2) toothpaste containing bioactive glass (5%). The samples underwent a 14-day remineralization/demineralzation cycle consisting of a twice-daily 30-minute submersion in a demineralizing solution (pH=4.52, 37°C) followed by a five-minute toothpaste treatment for the occlusal of the brackets. Each sample was analyzed using a polarized microscope, and AutoCAD 2007 was used to quantify the mineralization depth and area. Using SPSS version 23, the independent and paired t-tests were used to assess data statistically.

In terms of both remineralization area and depth, the study's findings revealed a statistically significant difference between the experimental and control groups (p < 0.001). Area and depth differences between the two remineralization methods in the treatment group were also statistically significant (p < 0.001).

Both sodium fluoride and bioactive-glass toothpaste are useful for remineralizing the WSL, while bioactiveglass toothpaste seems to work more effectively than sodium fluoride toothpaste.

 Colorless Silver Diamine Fluoride (SDF) solution is effective in increasing the remineralization of hard tooth tissue, and the silver ion in it has antibacterial properties. The aim of this study was to compare the effect of Sodium Fluoride (NaF) varnish with SDF on oral saliva pH in children with premature dental caries.

 In this clinical trial study, 25 children with the age group of 3-6 years old were randomly selected, and then randomly divided to two groups of receiving NaF and receiving SDF. The rate of salivary pH change in both study groups was measured and recorded before treatment (baseline time), 5, 10, 20, and 30 minutes after treatment, using digital pH meter. The results were evaluated using repeated measures and independent t-test at a significance level of 0.05%.

 The mean salivary pH level of the SDF group in the fifth (p = 0.018), tenth (p = 0.022), and twentieth (p = 0.039) minutes after treatment was significantly higher than it in the NaF group. In the SDF group, saliva pH at 5 (p = 0.001), 10 (p <0.001), 20 (p <0.001) and 30 minutes (p = 0.002) was significantly higher than the baseline time.

 The saliva pH of children increased significantly after using SDF compared to NaF. Due to the other positive effects of SDF in preventing caries compared to NaF, this substance is recommended for oral pH control in ECC and S-ECC treatment plans, especially in the cases where anesthesia is not possible.

Statement of the Problem:

 Every effort for increasing the calcium concentration in the saliva would be beneficial for prevention of dental caries. Regarding this issue, the natural products could be considered safer and more cost effective. Sesame is rich in calcium but the data about the effect of sesame on enamel roughness is inadequate.

This study aimed to assess the effect of an experimental sesame gel on the surface roughness of human enamel by using atomic force microscopy (AFM).

In the current experimental study, fifteen enamel slices with 1mm thickness were prepared. They were polished and acid etched to produce a substantial rough surface prior to the first AFM analysis. The enamel blocks were randomly divided into three groups treated with distilled water, fluoride gel, and an experimental prepared sesame gel correspondingly. The treating agent was applied for 3minutes at 0, 8, 24, and 48h intervals and washed by distilled water after each cycles. Ultimately, the final AFM micrographs were prepared. The statistical analysis was performed using paired t-test, one-way ANOVA, and Tukey Post Hoc tests (a=0.05).

Statistical analysis revealed that the surface roughness was significantly reduced in both sesame and fluoride groups (p= 0.017 and 0.018, respectively) while the control group (distilled water) were not noticeably changed (p= 0.12). The control group had statistically significant difference with both the sesame and the fluoride groups (p= 0.007 and 0.007, respectively) while the there was no significance difference between sesame and fluoride groups (p= 0.997). 

Following demineralization by acid etched process, the sesame gel significantly reduced surface roughness of enamel and its effect was similar to fluoride gel.

Background: This systematic review aimed to evaluate the efficacy of casein phosphopeptide‑amorphous calcium phosphate (CPP‑ACPF) varnish for remineralization of white spot lesions (WSLs) “in vitro” in human teeth. Materials and Methods: Literature search included three databases, namely Medline (via PubMed), The Cochrane Controlled Clinical Trials Register, and Google Scholar from 2010 to January 2021. The studies assessing WSL depth, calcium, phosphate ion release, and microhardness due to artificial demineralization or remineralization were considered for review. Reference articles were retrieved, and a customized risk assessment tool was used. The Cochrane risk of bias assessment tool was used to generate the risk of bias summary graph. Meta‑analysis was performed using RevMan 5.4. Heterogeneity was evaluated by Cochrane’s test, and random effects model was used to pool estimate of effect and its 95% confidence intervals (CIs) for surface microhardness. Results: Eighteen studies were selected for review based on the eligibility criteria. Four studies showed superior remineralizing effect of CPP‑ACPF compared to fluoride varnishes. Four studies involving 120 human permanent teeth samples were included in the meta‑analysis. Efficacy of CPP‑ACPF varnish was equivalent to other fluoride varnishes in improving surface microhardness after remineralization during 7‑day period (mean surface microhardness: 3.94, 95% CI [−9.08–1.21], I 2: 75%, P = 0.13). Major risks of bias associated with the studies included in the review were inadequate sample size, improper sample preparation, and unexplained blinding. Conclusion: CPP‑ACPF varnish appears to be equally effective as other fluoride varnishes in remineralizing artificially induced WSLs, but quality of evidence is low.

Different factors play a role in causing tooth decay. Modern dentistry is looking for a way to prevent tooth decay and suggests different ways to increase remineralization. The aim of this study was to evaluate the effect of nanohydroxyapatite toothpaste and mouthwash on remineralization of primary enamel lesions and obstruction of tubules.

In this study, 90 human extracted central teeth were used. Artificial decay was induced by placing the specimens for 72 hrs in a demineralization solution in an incubator at 37 °C. Hardness of the specimens was determined by a microhardness test before any surface treatment. The specimens were then divided randomly into 6 groups of 15. For 1-6 groups, the specimens were subjected to a pH cycling for 14 days, which included 3 hrs of demineralization and 21 hrs of remineralization. At the same time, 1-3 groups were in contact with diluted toothpaste in a ratio of 1:3 with deionized water at a rate of 5 ml twice a day for two min. Eac specimen of 4-6 groups was exposed to 5 ml of three types of mouthwashes for one min. Then, the specimens were cut in the buccolingual direction in order to simultaneously evaluate the dentinal tubules and Vickers microhardness test was performed again and the before and after microhardness values were compared. Then, the percentage of deposition on the surface and tubule obstruction were analyzed using SEM (Scanning electron microscope) (P=0.006). Data were analyzed using SPSS24 software bu the descriptive statistics, ANOVA analysis of variance, and multiple Tukey comparisons.

The results of analysis of variance test showed that there was a statistically significant difference in the microhardness changes between study groups (P=0.006). Also, in-group analysis of microhardness changes, there was a significant difference (P<0.0001). Besides, only in the group containing 0.5% nanohydroxyapatite toothpaste, more than 50% tubule deposition and obstraction was observed (P<0.05).

According to the present study, nanohydroxyapatite can be one of the treatment strategies to repair incipient lesions of teeth and the addition of nanohydroxyapatite, preferably to toothpaste, increases the dental microhardness.

Statement of the Problem:

Studies on the efficacy of erbium laser for enhancement of enamel resistance to acid attacks and its effects on fluoride uptake by the enamel are limited.

This study sought to assess and compare the effects of erbium-doped yttrium aluminum garnet (Er:YAG) laser irradiation and application of acidulated phosphate fluoride (APF) gel (alone and in combination) on remineralization of artificial white spot lesions (WSLs).

This in vitro, experimental study evaluated 90 buccal and lingual slabs of extracted human premolars. The specimens underwent pH cycling to induce WSLs. They were then randomly divided into 6 groups of caries-free positive control (c+), negative control with WSLs (ws), 1.23% APF gel applied on the enamel (F), Er:YAG laser irradiation (80 mJ, 10 Hz, and 8 J/cm2) of enamel (L), APF gel application followed by laser irradiation (FL), and laser irradiation followed by fluoride gel application (LF). The fluoride ion content of specimens was measured before and after the intervention using a potentiometer. Data were analyzed by ANOVA (p < 0.05).

APF gel application before/after laser irradiation maximally increased the fluoride uptake by the enamel (p = 0.000). Application of APF gel in group F and laser irradiation in group L increased fluoride uptake by the enamel, compared with groups 1 and 2 (p = 0.000). Laser- treated (L) and APF-treated (F) groups had no significant difference in this respect (p = 0.945). Maximum fluoride concentration was noted in combined laser and fluoride groups (FL=332.07ppm and LF=341.27ppm) with no significant difference between the two (p = 1.000).

Er:YAG laser irradiation changes the chemical composition of enamel and probably promote its remineralization, especially when combined with APF gel application, which highlights its cariostatic potential.

Background:

 Dental caries occurs due to the imbalance in the course of demineralization-remineralization in favor of demineralization. With remineralization, some signs of halted progression could be observed in the primary stages. The aim of this study is to in-vitro investigation of the effect of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP), fluoride varnish, and fluoride gel on the microhardness of the enamel of permanent teeth.

In this in vitro study, 344 specimens prepared from human premolars were randomly divided into four groups (n=86 specimens in each group). After preparing the specimens, the initial enamel value was first evaluated by Vickers microhardness technique. The specimens were immersed in a demineralizing solution and then treated with remineralizing compounds (I. Control, II. Fluoride varnish, III. Fluoride gel and IV. CPP-ACP). Microhardness values were re-measured at the end of demineralization and remineralization stages. The data were analyzed using SPSS software (version 16.0).

The results of the present study indicated a statistically significant difference in all groups in terms of initial enamel microhardness (0.288 ± 42.77), remineralization (0.213 ± 26.89), and demineralization values (0.167 ± 63.87) (p <0.001). Besides, there was a statistically significant difference in different groups in terms of remineralization values (p <0.001). In other words, the mean microhardness was 241.21 ±15.60 in Group III, 221.12 ± 75.69 in Group II, 211.16 ± 43.04 in Group IV, and 178.21 ± 63.9 in group- I.

Conclusion :

All remineralizing compounds resulted in enhanced microhardness of the enamel. Nevertheless, the fluoride-containing products showed greater potential in improving the level of microhardness and strength of the teeth compared to the compounds containing CPP-ACP.

Preventing caries or stopping primary caries lesions is one of the most important goals of oral health care. The aim of this study was to compare the effect of flavonoids and the whey extract on tooth enamel by measuring the microhardness of enamel following its demineralization.

In general, 42 samples of healthy enamel were prepared in this experimental-laboratory study. After measuring the initial microhardness using Vickers hardness tester )Buehler, Lake Bluff, IL, USA(, the samples were immersed in the demineralization solution for 4 days and re-tested for microhardness. Then, the samples were randomly divided into three groups of 14 and treated, including the grape seed extract w/v8%, the whey extract, and the artificial saliva. The samples were in contact with the treatment material for 8 days in an incubator at 37° C and then their microhardness was evaluated again.

The analysis of covariance showed that there was a significant difference in the amount of microhardness changes between the study groups (P<0.001) so that microhardness changes were the highest in the grape seed extract group while they decreased in the control group. However, the post hoc test showed no statistically significant difference between the whey extract and grape seed extract groups (P=1.000). Finally, the control group had a statistically significant difference from the whey extract and grape seed extract groups.

Both grape seed extract and whey extract increased remineralization and the microhardness of tooth enamel compared to the control group. Thus, these two substances can be considered as natural and effective substances in the non-invasive treatment of carious lesions.

Preventing caries or stopping primary caries lesions is one of the most important goals of oral health care. The aim of this study was to compare the effect of flavonoids and the whey extract on tooth enamel by measuring the microhardness of enamel following its demineralization.

In general, 42 samples of healthy enamel were prepared in this experimental-laboratory study. After measuring the initial microhardness using Vickers hardness tester )Buehler, Lake Bluff, IL, USA(, the samples were immersed in the demineralization solution for 4 days and re-tested for microhardness. Then, the samples were randomly divided into three groups of 14 and treated, including the grape seed extract w/v8%, the whey extract, and the artificial saliva. The samples were in contact with the treatment material for 8 days in an incubator at 37° C and then their microhardness was evaluated again.

The analysis of covariance showed that there was a significant difference in the amount of microhardness changes between the study groups (P<0.001) so that microhardness changes were the highest in the grape seed extract group while they decreased in the control group. However, the post hoc test showed no statistically significant difference between the whey extract and grape seed extract groups (P=1.000). Finally, the control group had a statistically significant difference from the whey extract and grape seed extract groups.

Both grape seed extract and whey extract increased remineralization and the microhardness of tooth enamel compared to the control group. Thus, these two substances can be considered as natural and effective substances in the non-invasive treatment of carious lesions.

The aim of this study is to quantitatively evaluate the remineralization potential of three remineralizing systems as follows: fluoride, casein phosphopeptide‑amorphous calcium phosphate (CPP‑ACP), and CPP‑ACP with fluoride, under scanning electron microscope with energy‑dispersive X‑ray analysis.

In this in vitro study A total of 40 enamel specimens were prepared from the buccal or lingual surfaces of human premolars extracted for orthodontic reason. Specimens were then placed in demineralizing solution for 96 h, to produce artificial caries‑like lesion. Calcium and phosphate weight percentage of demineralized specimens was measured. Specimens were divided into four groups as follows: (a) control, (b) CPP‑ACP, (c) CPP‑ACP with fluoride, and (d) fluoride varnish. Except for the control group, the entire specimens were subjected to remineralization using respective remineralizing agents of their groups. The prepared specimens were assessed for calcium and phosphate weight percentage using scanning electron microscopy‑energy dispersive X‑ray spectroscopy. One way analysis of variance (ANOVA), followed by Tukey’s test, was performed with the help of critical difference (CD) or least significant difference at 5% and 1% level of significance. P ≤ 0.05 was taken to be statistically significant and P < 0.001 as statistically highly significant.

The mean weight percentage of calcium and phosphorus of specimens treated with CPP‑amorphous calcium phosphate nanocomplexes plus fluoride (ACPF) was significantly higher than other groups.

All the groups showed statistically significant remineralization. However, because of added benefit of fluoride, CPP‑ACPF showed statistically significant amount of remineralization than CPP‑ACP