جستجوی مقالات مرتبط با کلیدواژه « saliva » در نشریات گروه « پزشکی »

Oral candidiasis is the most common infection of oral mucosa caused by Candida albicans. A common predisposing factor for candidiasis is immune system suppression in specific diseases such as AIDS and various cancers. This study aimed to analyze the effect of Kefir probiotic products on the count of C. Albicans in the saliva of chemotherapy patients.

In this single-blinded clinical trial, 50 patients were selected who have signed informed consent forms. Patients aged 20-60 years with colon or breast cancer who received the same chemotherapy regimen were included and those with a history of radiation therapy, underlying diseases, using antibiotics, anti-fungal and GCSF medicines were excluded. Matched patients in test and control groups received 100 ccs Kefir probiotic and mineral water, respectively at a specific daily time for five weeks. Blood and saliva samples were collected in five steps. Data were analyzed using SPSS Version 21 and the significance level was set at p< 0.05.

In saliva samples, the count of C. Albicans in the test group dropped significantly (p<0.05), while there were no significant differences between test and control groups in blood samples (p>0.05). Comparing the follow-up sessions, in the test groups, WBC and Neutrophil, and in the control groups, WBC count and hematocrit showed significant differences (p< 0.05).

Based on the results, using probiotic products daily over a short-term period drops the count of oral C. Albicans. Therefore, Kefir probiotic products can be used as an additional treatment for chemotherapy patients.

Oral cancer is the sixth most common cancer in the world and the third most common cancer in developing countries. Early detection of oral cancer can reduce mortality in several ways. The aim of the present study was to combine the quantitative results of various studies concerning serum and salivary microRNAs for early diagnosis of head and neck squamous cell carcinoma (HNSCC).

This systematic review and meta-analysis was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Guideline. We searched all the relevant English studies in international databases of PubMed/Medline, Scopus, Web of Science, Cochrane, ProQuest, Embase, and Wiley until February 2022. A random-effects model was used to estimate the pooled odds ratio (OR) and its corresponding 95% confidence interval (CI) for each study. A total of 672 articles were found. After screening, 93 articles were approved for systematic review. Finally, 5 completely relevant articles were examined in the meta-analysis.

Considering all studies regarding miRNAs, the combined results indicated that AUC  =  0.73, with a sensitivity of 71.68% and a specificity of 69.95%, could be used for HNSCC diagnosis. Due to the moderate sensitivity and specificity of miRNAs, they may be able to confirm or exclude suspected cases of this disease, enhancing their utility as clinical diagnostic indicators.

The available data provide evidence that miRNAs, especially MiR-31 expression in the saliva, serum, or plasma, can be used as a diagnostic biomarker for HNSCC patients. However, controlled clinical trials with large sample sizes are needed to validate different miRNAs.

Inflammatory bowel disease (IBD) is a chronic relapsing inflammatory disorder of the gastrointestinal tract that affects millions of people worldwide. Since acetylcholine is one of the effective factors in reducing inflammation and considering the benefits of using saliva, in this study, the amount of cholinesterase activity in saliva and serum in patients with IBD and healthy people was investigated.

Thirty patients with IBD who were referred to Imam Reza Hospital, as well as 30 healthy individuals, participated in this study. Saliva and serum samples were collected in the morning. Cholinesterase activity was evaluated using the photometric method, and data were analyzed using an unpaired Student’s t-test or Mann-Whitney test.

The mean activity of cholinesterase and saliva flow rate in saliva and serum were not significantly different between the two groups. Xerostomia inventory score was significantly higher in the IBD group (P<0.05).

It seems that cholinesterase activity does not change in patients with IBD, but patients feel more dry mouth than healthy people.

This study aimed to investigate the effects of two types of gum, containing xylitol or casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), on saliva properties.

In this single-blind clinical trial, 60 dental students were randomly divided into three groups (n=20). Each group was instructed to chew one of the following substances: paraffin (control), xylitol gum, or CPP-ACP gum. Saliva samples were collected before and five minutes after chewing. Saliva volume was measured using a pipette, and saliva pH was evaluated with a digital pH meter. The calcium concentration was determined using the photometric method, while phosphorus concentration was measured via spectrophotometry. Statistical analysis was performed using the chi-square test, ANOVA, Tukey HSD post hoc test, and paired t-test (α = 0.05).

Chewing all three substances significantly increased saliva volume and pH while reducing phosphorus ion concentration (P < 0.05). The calcium ion level increased significantly after five minutes of chewing the CPP-ACP gum (P = 0.002). After the intervention, between-group comparisons revealed no significant difference in saliva volume and pH (P > 0.05). However, the calcium level in the CPP-ACP group was significantly higher than the control group (P = 0.01). Additionally, the phosphorus ion level was significantly lower in the xylitol gum group than in the control and the CPP-ACP groups (P < 0.05).

Chewing CPP-ACP gum may promote oral health more effectively than xylitol gum by increasing salivary calcium levels and less affecting phosphorus levels.

Hashimoto's thyroiditis (HT) is one of the common causes of hypothyroidism. Although various factors are involved in its development, recently the role of oxidative stress in its pathogenesis has been known. The present study aimed to investigate the level of total antioxidant capacity (TAC), catalase (CAT), and salivary superoxide dismutase (SOD) in patients with HT compared with the control group. The present case-control design included patients aged 18-80 years suffering from HT referred to the endocrine clinic. Eligible patients were selected by the available sampling method. Complete unstimulated saliva was collected under a rest state in a comfortable room between 10:00 AM and 12:00 AM and a checklist was used to collect data. The chi-square, t-test, and Mann-Whitney U tests were used for data analysis using SPSS 22 software. The mean age of the participants was 36.55±9.37 years (range: 20-56). The two groups were the same in terms of age and gender (P>0.05). The findings indicated that the difference in the means CAT between the two groups was 22.63 which was strongly and statistically significant (P<0.001). In this study, the level of TAC and SOD in Hashimoto's thyroid patients was decreased and the level of CAT was increased. These initial findings show that oxidative stress can be associated with Hashimoto's thyroid disease or the possibility of developing this disease increase.

Iron deficiency anemia is a prevalent form of anemia, particularly among women. Diagnosing and tracking this condition typically requires various measurements of serum iron, ferritin, and total iron-binding capacity (TIBC) through blood testing.

This study aims to evaluate the utility of saliva as a predictive marker for monitoring iron levels in iron deficiency anemia by comparing and correlating iron, ferritin, and TIBC levels in the serum and saliva of anemic and healthy women.

In this comparative analysis, 40 women aged 20 - 40 with a similar periodontal status (anemic subjects = 20 and healthy subjects = 20) were examined. After a complete hematological and oral examination, salivary samples were collected using the spitting method, and iron, ferritin, and TIBC levels were measured and compared to the serum levels. Data were analyzed using SPSS 24.

The study results showed that the differences in saliva iron and ferritin levels were insignificant between the two groups (P-value = 0.07 and 0.26, respectively), but those were statistically significant in the serum (P-value = 0.0001 and 0.0001, respectively). Additionally, a significant difference was found in the saliva TIBC level (P-value = 0.001), which was negligible in the serum (P-value = 0.118).

The results underscore the efficacy of integrating both serum and saliva analyses for comprehending iron-related parameters. While saliva analysis cannot entirely substitute for serum analysis, it represents a convenient approach that may serve as an initial step or be employed in large-scale anemia screenings.

Systemic sclerosis (SSc) is a rare chronic inflammatory disorder characterized by diffuse fibrosis and vascular abnormalities in the skin and internal organs. Interleukin-23 (IL-23) is a pro-inflammatory cytokine that can enhance the expansion of T helper 17 (Th17) cells and thus plays a critical role in many inflammatory autoimmune diseases. This study aimed to assess the salivary IL-23 levels in Iranian patients with SSc compared to healthy individuals.

In this cross-sectional study, unstimulated saliva samples (5 cc) were collected from 88 SSc patients and 88 age- and sex-matched healthy individuals. The salivary levels of IL-23 in the saliva samples were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit.

The mean salivary levels of IL-23 in the patient group were significantly higher than in the control subjects (164.5 ± 22.1 ng/L vs. 95.8 ± 15.7 ng/L, P < 0.0001). In SSc patients, the salivary IL-23 levels were significantly elevated in ACA-positive compared to ACA-negative participants (179.8 ± 11.2 ng/L vs. 144.3 ± 15.7 ng/L, P < 0.0001). However, IL-23 was not associated with gender or age (P > 0.05).

The results suggest that IL-23 is associated with the pathogenesis of SSc; therefore, this pro-inflammatory cytokine is not only a valuable supportive biomarker for monitoring the disease progression but also blocking IL-23 could be considered a potential therapeutic target, especially in early SSc. Further comprehensive studies are needed to confirm our findings.

This study aimed to measure bone-specific alkaline phosphatase (B-ALP) levels in saliva and determine how they relate to different stages of skeletal development, as assessed by hand-wrist X-rays using the Hagg and Taranger method. Since orthodontic treatment is closely linked to an individual’s growth, the development of the jaws, face, and overall body, and how they affect the alignment of teeth, the suggestion to use biomarkers to evaluate an individual’s skeletal maturity has emerged as a promising approach. Unlike traditional radiographic methods, which are subjective and based on morphology, biomarkers provide objective indicators related to the patient’s physiology, and they do not involve radiation exposure or magnification errors. The main goal of this study was to measure B-ALP levels in saliva and examine their correlation with varying degrees of skeletal maturity. In a positive correlation, the total ALP present in saliva could be a valuable biological indicator in growing patients.

Thirty patients were randomly selected for the study based on the inclusion criteria: Age of the individual: 9‒19 years, with good general health and no nutritional issues. A sample of unstimulated whole saliva was collected using a passive drooling method to estimate levels of the bone alkaline phosphatase using an enzyme-linked immunosorbent assay (ELISA) kit. After saliva collection, hand-wrist radiographs were immediately obtained and manually traced onto 50-micron-thick lead acetate tracing sheets using an 0.5-mm lead pencil. The hand-wrist radiographs were then categorized into five groups, which involved analyzing the morphology of the hand-wrist radiographs: group SO (prepubertal), group S (pubertal onset), group MP3G (peak pubertal), group DP3 (pubertal deceleration), and group RJ (growth completion).

There were significant differences between the B-ALP levels between different skeletal ages. The salivary B-ALP values of the group MP3 were significantly higher than those of groups SO and RJ. The mean salivary B-ALP levels consistently increased from the group SO to the group MP3, followed by a gradual decrease.

Salivary alkaline phosphatase activity was significantly higher during the peak pubertal period (group MP3) compared to both the pre-peak (group SO) and post-peak (group RJ) values. This finding suggests that salivary bone alkaline phosphatase can be used as an additional indicator alongside conventional skeletal maturation indicators to assess pubertal development.

In this article published in Cell J, Vol 17, No 1, 2015, on pages 159-162, the authors found that the affiliation of secondauthor in address 2 was missed during the formatting of the paper. Therefore, we corrected it.The authors would like to apologize for any inconvenience.

Recurrent oral ulcers constitute the most common complaint of patients with Behçet’s disease (BD) and recurrent aphthous stomatitis (RAS). Enhanced innate immune response and neutrophilic activity might be a possible etiopathogenesis of BD. This study aimed to determine the significance of salivary HNP 1-3 in BD and RAS patients and detect their correlation with different clinical presentations, disease activity, and characteristics of oral ulcers.This cross-sectional study included 25 BD patients and 25 RAS patients as well as 25 healthy participants. 5 cc of unstimulated saliva were collected and levels of HNP 1-3 were measured by ELISA. Other data were obtained through interviews, examination, and reviews of medical records. SPSS 25.0 was used to analyze the data.Salivary HNP 1-3 levels were not significantly different between the study groups (P=0.282). Duration of oral ulcers did not correlate with HNP 1-3 levels in RAS and BD patients (P>0.05). Also, BD patients with involvements other than oral ulcers were not found to have different levels of HNP 1-3 comparing to those who did not manifest these conditions.The validity of HNP 1-3 to be used as a probable biological marker for evaluation, diagnosis, and estimation of disease activity in patients with BD and RAS is still questionable due to our results.

Saliva, as a complex fluid composed of secretory products from salivary glands, plays different roles in the oral cavity. Saliva has potentially been used for the diagnosis of viral, bacterial, and systemic diseases in the last few years. Individuals with minimal training can collect saliva as a diagnostic fluid easily and non-invasively. Saliva is a simple and economical method for screening the public. However, saliva has various levels of variability and instability. A systematic search of published literature was performed on Medline using PubMed. Several keywords, including saliva, systemic disease, disease, health, and diagnosis, were used in this search. This review discusses the diagnostic potential of salivary systemic diseases such as infectious, malignant, and other systemic diseases.

The present study aimed to investigate the relationship between stress and temporomandibular disorders (TMDs) by quantification of occupational stress using the Dental Environment Stress (DES) questionnaire as well as measuring the stress markers in the saliva, including cortisol and immunoglobulin A (IgA), in fourth to sixth-year dental students of Qom Dental School.

In the present study, 60 students were included. A clinical examination was first performed using the Research Diagnostic Criteria/Temporomandibular Disorder (RDC/TMD) criteria in order to determine the temporomandibular joint (TMJ) status, and then the DES questionnaire was completed in order to determine the occupational stress level of dental students. Finally, saliva samples were obtained from the students, and the levels of salivary cortisol and IgA were measured using enzyme-linked immunosorbent assay (ELISA). Independent t-test, ANOVA, Chi-square test, and Pearson's correlation coefficient were used for data analysis (alpha=0.05).

The mean salivary levels of cortisol and IgA were found to be 4.74 ± 2.80 ng/mL and 220.26 ± 121.22 µg/mL, respectively. The mean DES score was 2.48 ± 0.58. Of all students, 24 (40%) suffered from TMDs. There was no correlation between cortisol and IgA levels and the DES score (P>0.05). No significant relationship was found between TMD and salivary cortisol, salivary IgA, or DES score (P>0.05).

Stress is one of the most important causes of TMDs. Although salivary biomarkers are known as useful markers for detection of stress in patients with TMDs, this issue was not confirmed in the present study.

The hormonal changes associated with puberty, menstruation, pregnancy, and menopause exert varying effects on the saliva and oral health of women. The aim of this study was to investigate the effect of pregnancy and menopause on saliva (pH and flow rate) and oral health utilizing saliva sampling and oral examination.

This case-control study was performed on 120 women in three groups of pregnant women, menopause and control group (each group = 40). Subjects were evaluated for any oral lesions and were examined for DMFT, gingival index (GI) and community periodontal index (CPI), and their salivary flow rate and pH were measured. Data analysis was performed using descriptive statistics, Analysis of Covarience, partial Spearman’s correlation coefficient, adjusted logistic re-gression.

According to the ANCOVA, there was a significant difference among groups based on the pH and salivary flow rate. The highest and lowest pH has been observed in menopausal (6.80±0.42) and pregnant (6.02±0.5) group, respectively. The mean salivary flow rate was highest in the pregnant group (2.91±0.92) and lowest in the menopausal group (2.12±0.85). There was no significant difference among the groups in terms of DMFT, CPI and GI after adjusting the effect of age as covariate. There was a significant difference between the three groups in terms of xerostomia and hali-tosis (p <0.05).

Both pregnancy and menopause lead to alterations in oral health. In this investigation, the metrics of pH, xerostomia, and halitosis exhibited higher values in the menopausal group compared to the pregnant group.

The type of diet is one of the important causes of caries and one of the techniques to evaluate the carcinogenicity of foods is to measure the pH of saliva after consuming them. This study aimed to measure the pH of artificial saliva after exposure to a series of common snacks in the community. In this laboratory study, all samples (including 18 common snacks in the community) were completely crushed. Then, 8 g of each sample was dissolved in 50 ml of artificial saliva and continuously mixed with a magnet in a human. The pH of the solutions was then measured by a digital pH meter at intervals of 0, 1, 2, 4, 6, 8, and 10 minutes after the onset of dissolution. Statistical analysis was performed with related tests and SPSS statistical software version 22. The pH of all groups except chocolate and whole wheat biscuits decreased significantly during 10 minutes (P-value <0.05). The obtained results indicated that the groups lowered the pH of artificial saliva below the critical pH (5.5) including vinegar chips, peanut vinegar, cherry industrial dish, home-made dish, industrial swallow, house swallow, and plum. The pH level of the other groups was higher than the limit was critical. The snack with the least pH change and the snack with the most and most severe pH change were whole-grain biscuits and homemade desserts, respectively. All snacks tested except chocolate and whole wheat biscuits significantly reduced the pH of artificial saliva. The pH drop was high for acidic groups.