جستجوی مقالات مرتبط با کلیدواژه « stool » در نشریات گروه « پزشکی »

Escherichia albertii is a gram-negative and facultatively anaerobic bacterium that has been isolated as a causative agent of diarrhea in recent years. Due to the lack of sufficient information on the phenotypic and biochemical characteristics of this bacterium, it is difficult to distinguish it from other species of the Enterobacteriaceae, especially Escherichia coli pathotypes. So, the aim of this study was to evaluate the prevalence of E. albertii in the urine and stool samples of patients with urinary tract and gastrointestinal tract infection in Kermanshah hospitals using culture and molecular methods.

Four hundred samples consisted of 200 urine and 200 stool samples confirmed as Enterobacteriaceae phenotypically were collected. After subculture and biochemical tests, finally white colonies on XRM-McConkey agar (suspected to be E. albertii) were selected and subjected to PCR using specific primer for cdt gene (Eacdt). SPSS version 16 software was used for the statistical analysis of the data, and the p-value less than 0.05 was considered statistically significant.

Only 5 samples (1.25%) were positive in the final culture on XRM-MacConkey agar. None of them were then confirmed as E. albertii by the PCR.

According to the phenotypic isolation of this bacterium in present study and previous studies on the prevalence of it, it seems that the use of Eacdt specific primer is not efficient for the detection of this bacterium. However, further studies are recommended to confirm it.

Colorectal cancer (CRC) is the third most prevalent cancer with approximately 9,000 annual deaths worldwide. However, early detection can provide a high survival rate. The fecal DNA, as a non-invasive method for detecting the genetic markers, such as the TP53 gene, can be conducive to disease diagnosis. In this study, we aimed to investigate the presence of the TP53 mutations in the stool samples and their relationship with somatic mutations in the tissue samples of CRC patients from northwestern Iran. In the present cohort study, tumor and stool samples were obtained from 64 CRC patients (mean age of 60), who were undergoing surgery. Total genomic DNA was extracted from the tissue and stool samples, and TP53 mutations were detected using the PCR-SSCP method, followed by direct sequencing. Differences between mutations were observed in the tumors, and the stools were examined using the McNemar method. Of 64 CRC patients, 19 individuals (30%) demonstrated 27 point mutations in exons 5-7 of the TP53 in the tumor samples. Furthermore, analysis of the stool specimens revealed that the 22 mutations (81.5%) identified in the tumor specimens were also present in the stool of 12 patients (P = 0.063). Based on the results, the DNA from the tissue could be replaced with fecal DNA in the mutation detections for CRC. Given the non-invasive nature of fecal sampling, it can be desirable and acceptable for patients in molecular screening tests as it increases the screening rates and improves timely CRC diagnosis.

The present study implemented an RT-qPCR assay for the detection and quantification of human cosavirus in stool specimens from pediatric patients involved in acute gastroenteritis.

Human cosavirus is a newly recognized virus that seems to be partly related to acute gastroenteritis in pediatric patients. However, the relationship between human cosavirus and diseases in humans is unclear

From January 2018 to December 2019, a total of 160 stool samples were collected from pediatric patients presenting with acute gastroenteritis in a hospital in Karaj, Iran. After viral RNA extraction, RT-qPCR was performed to amplify the 5’UTR region of the human cosavirus genome and viral load was analyzed. Results The human cosavirus genomic RNA was detected in 4/160 (2.5%) stool samples tested. The maximum viral load was determined to be 4.6×106 copies/ml in one sample obtained from a 4-year-old patient.

The human cosavirus as a new member of the Picornaviridae family was illustrated in fecal samples from pediatric patients with acute gastroenteritis in Iran. This is the first documentation of human cosavirus circulation in Iranian children.

Ascaris worm, as one of the commonest helminthic infections, constitutes a major public health challenge and concern in the majority of developing countries. This study was conducted to assess the prevalence of Ascaris worm infection and its associated risk factors among primary school children in Lambata community to determine the prevalence of Ascaris infection, age, gender and associated risk factors among them to create awareness and effective management program. This is a cross-sectional descriptive study conducted between January 2019 and November 2020, in nine selected primary schools in Lambata community. A total of 303 stool samples were collected using random sampling to determine the prevalence of Ascaris infection using stool smear technique. The socio-demographic data were collected, using a structured interview questionnaire. The collected data were analyzed using simple percentages, OR and chi-square analytical methods. Out of the 303 screened stool samples, 156 (51.5%)  had Ascaris infection. The most infected age-groups were 11-12 years old (73.8%; OR = 2.11) and 9-10 years (57.1%; OR = 2.01), while 6-8 year old subjects had the lowest rate (42.3%; OR = 1.00) of infection. Males (65.9%; OR= 2.00) were more infected than their female (39.9%; OR = 0.09) counterparts (p <0.05). Age, educational status / occupational status of parents, and defecation habits were significantly(p <0.05) associated with the prevalence of Ascaris infection. With the overall prevalence of 51.5% of Ascaris infection among the subjects, there is an indispensable need for health education promotion and coordinated de-worming of the primary school children in this community

Cytomegalovirus (CMV) infection is the most common viral opportunistic infection causing gastrointestinal diseases such diarrhea and colitis in immunocompromised patients. The development and performance of a robust and sensitive PCR assay are usually evaluated to detect CMV DNA in human fecal specimens. In this study, our aim was to detect CMV DNA in stool samples taken from patients with HIV/AIDS, cancer, and transplant recipient patients with chronic and persistent diarrhea using a non-invasive method.
A total of 633 immunocompromised patients (451 males and 182 females) suffering from persistent or chronic diarrhea were included in this study. Among them, 392 were HIV/AIDS patients, 151 had cancer and were receiving chemotherapy, and 90 were recipients of a solid organ or bone marrow transplant. CMV genome was extracted from the stool samples using phenol: chloroform: isoamyl alcohol method. CMV DNA was identified by polymerase chain reaction using sequence specific primers on genomic DNA.
Looking at the frequency of CMV DNA in 392 HIV/AIDS patients, we found that only 5 patients (1.27%) were positive for CMV genome, while this frequency was 4.63% (7/151) and 5.5% (5/90) in patients with cancer receiving chemotherapy and in those with solid organ or bone marrow transplant, respectively.
The results of this study revealed that the cause of chronic or persistent diarrhea in HIV/AIDS, cancer, and graft recipient patients might be related to CMV infection. Accordingly, we recommend a non-invasive method, such as stool sample, as a first line of diagnosis of enteritis when the physician suspects that a patient has CMV infection.

Most cancer studies focus on exploring non-invasive biomarkers for cancer detection. In the present study, we sought to investigate the expression level of microRNA-21 (miR-21), as a potential diagnostic marker, in serum and stool samples from 40 patients with colorectal cancer (CRC) and 40 healthy controls.
Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 in serum and stool. At the same time, the sensitivity and specificity of this marker was evaluated by receiver operating characteristic (ROC) curve analysis.
miR-21 expression levels of serum and stool were up-regulated 12.1 (P The results of this study indicated that miR-21 expression levels in serum and stool can be considered as a potential diagnostic biomarker for the diagnosis of CRC patients. However, more studies are required to confirm the validity of miR-21 as a valuable non-invasive diagnostic tool for CRC.

Among the most important parasitic disease, causing diarrhea, Gi­ardia lamblia is noteworthy. Nowadays detection methods for these parasites in­clude parasitological methods such as microscopic examination. The sensitivity of these methods relies on the expertise and experience of examiners. In contrast, molecular methods such as PCR are less dependent on the expertise of the exam­iner. Here we developed a PCR for the detection of G. lamblia genome in stool samples in comparison with microscopy, which is the gold standard.
For the evaluation of primers, 22 positive samples and 47 negative samples were used. QIAamp DNA Stool Mini Kit (QIAGEN, Germany) was used for DNA extraction from feces. Primers for PCR were designed using Primer-BLAST which uses Primer 3 to designing specific primers (NCBI/ Primer-BLAST).
Sensitivity of the PCR was done with 100% (95%CI: 84.56-100) for the detection of G. lamblia DNA isolated from patients stool samples which were posi­tive for G. lamblia cysts and/or trophozoites using microscopy as gold standard. In comparison with microscopy, PCR had showed the specificity of 97.87% (95%CI: 88.71-99.95).
We designed new primers for the Giardia, and PCR method for the rapid and accurate identification of Giardia parasites established. With considera­tion to the routine diagnosis techniques in medical parasitology and their limita­tions such as time consuming, laborious, less sensitivity etc. This G. lamblia PCR is a sensitive and specific application for the diagnosis of G. lamblia and provides us a reliable method in the routine intestinal parasitic infection laboratory diagnosis.