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عضویت

جستجوی مقالات مرتبط با کلیدواژه « storage » در نشریات گروه « پزشکی »

  • Leila Nateghi*, Elahesadat Hosseini, Fatemehsadat Mirmohammadmakki
    Background and Objectives

    Potatoes (Solanum tuberosum), as starchy plants, have been highly esteemed for their rich supply of nutrients. Numerous studies have investigated the potential health benefits of potatoes and explored potential solutions. Among these considerations, the discussion regarding microbial contaminants has remained an important topic.

    Materials and Methods

    The present study used cold atmospheric plasma (CAP) to evaluate the microbial quality (includ- ing mesophilic and psychrotrophic bacteria and mold and yeasts) of raw potato slices during a 14-day storage period. To achieve this goal, the duration of CAP exposure was set at 5, 10, and 15 min, utilizing an electric voltage of 60 kV and a specific frequency of 20 kHz.

    Results

    The findings revealed the effectiveness of CAP pre-treatment in inhibiting microbial growth over the 14 days when compared to the control sample (untreated sample), with a statistically significant difference (P < 0.05). Moreover, with an extension of the CAP exposure duration to 15 min, there was a significant reduction in the logarithmic count of mesophilic, psychrotrophic microorganisms, molds, and yeasts (4.95, 2.85, and 2.22CFU/g, respectively) in comparison to the control groups (7.5, 5.62, and 5.5CFU/g) on days 0, 7, and 14 of the storage periods (P < 0.05).

    Conclusion

    The results of this study highlight the potential of CAP pre-treatment on reducing the microbial load in raw potato slices prior to frying, which could potentially influence the overall quality of potato-based products.

    Keywords: Bacteria, Cold plasma, Fungi, Storage, Solanum tuberosum}
  • Nastaran Akbarieh, Parisa Sadighara, Nabi Shariatifar, Ebrahim Molaee-Aghaee, Ramin Aslani

    Butter is popularly known in dairy products and consists of large amounts of milk fat (at least 80%). The risk of oxidation increases by a higher content of unsaturated fatty acids in milk fat and adversely affects the quality and safety of the product. Primary oxidation products are hydroperoxides, which can decompose to form lower molecular weight compounds such as alcohols, aldehydes, free fatty acids (FFA), and ketones. This process may give rise to butter rancidity. In this study, 20 samples of traditional butter, including 10 samples of cow butter and 10 samples of sheep butter, were purchased from different traditional dairy product supermarkets in southern Tehran and were analyzed to evaluate the amount of fat oxidation. The peroxide levels in all samples of traditional butter were within the permissible limit established by Iran's national standard. According to the results, it was found that the amount of peroxide in cow butter (1.08 ± 0.12 mEq/kg) is higher than in sheep butter (p=0.037). It may be related to the temperature and time of storage. Therefore, strategies such as diminishing temperatures, maintaining products in a dark environment, removing oxygen by creating a vacuum in the packaging, and using antioxidant compounds can prevent butter oxidation.

    Keywords: Butter, Lipid oxidation, Free fatty acids, Hydroperoxide, Peroxide value, Storage}
  • Ramin Salyari, Esmaeil Seifi *, Feryal Varasteh, Mahdi Alizadeh
    Peach is one of the most important horticultural crops and its storage and postharvest issues are increasingly taken into consideration. This experiment was conducted to the assessment of salicylic acid spray at 0, 1, 2, and 4 mmol concentrations, and salicylic acid submergence in 2 mmol concentration on the post-harvest quality of peach cultivar Robin, and the measurement in the four storage times in 2014-2015. In this experiment, the weight loss, volume decrease, length decrease, diameter decrease, pH, total acidity, total soluble solids, vitamin c, antioxidant capacity, total phenol, total flavonoid, and fruit Peach is one of the most important horticultural crops and its storage and postharvest issues are increasingly taken into consideration. This experiment was conducted to the assessment of salicylic acid spray at 0, 1, 2, and 4 mmol concentrations, and salicylic acid submergence in 2 mmol concentration on the post-harvest quality of peach cultivar Robin, and the measurement in the four storage times in 2014-2015. In this experiment, the weight loss, volume decrease, length decrease, diameter decrease, pH, total acidity, total soluble solids, vitamin c, antioxidant capacity, total phenol, total flavonoid, and fruit firmness were measured. The results suggested that the salicylic acid treatment caused the inhibition of the weight losses, volume decrease, length decrease, and diameter decrease over the storage period and maintained the fruit firmness. The best result obtained from 2 mmol spray of salicylic acid before harvest. The lowest total soluble solids and pH were observed in 2 mmol salicylic acid spray in all the storage times, while in the other treatments such as control, their contents increased. The results also demonstrated that the highest vitamin C content was in 2 and 4 mmol salicylic acid spray and 2 mmol salicylic acid submergence treatment, and the highest total phenol content resulted from 15 days after storing in all the salicylic acid treatments. The treatment of salicylic acid did not have much effect on the total flavonoid content and antioxidant capacity. Among all the treatments, the spray of 2 mmol salicylic acid, two weeks before the fruit harvest, revealed the best result and can be considered in the enhancement of peach postharvest shelf life.
    Keywords: Antioxidant capacity, Prunus persica, shelf life, Storage, Total phenol}
  • سولماز عالی پور، ناصر همدمی*، محسن دلوی اصفهان، سعیده فلاح، جوشقانی
    سابقه و هدف

    روش های مختلفی برای کاهش تلفات در حین انبارداری میوه ها و سبزیجات به کار گرفته شده است که در میان آنها، کاربرد صنعتی میدان الکتریکی با ولتاژ  بالا مناسب تر به نظر می رسد. هدف از این مطالعه بررسی اثرات میدان الکتریکی ولتاژ بالا (HVEF) بر چهار ویژگی اصلی کیفی انار (Punica granatum L.) در طول انبارداری بود.

    مواد و روش ها

    میوه های انار در معرض میدان الکتریکی با قدرت 2 و 4 کیلو ولت بر سانتی متر و اعمال میدان در روز اول انبارداری و در فواصل زمانی منظم طی انبارداری قرار گرفتند. ویژگی های کیفی میوه از قبیل رنگ، بافت، فعالیت آنتی اکسیدانی و سرعت تنفس در فواصل زمانی هر پانزده روز یکبار به مدت شصت روز ارزیابی شدند.

    یافته ها 

    نتایج نشان دادند که سرعت تنفس میوه های انار با افزایش زمان ذخیره سازی کاهش یافت و نمونه های تیمار شده با میدان الکتریکی سرعت تنفس کمتری نسبت به نمونه کنترل داشتند. شاخص روشنایی پوست انارهای تیمار شده تحت میدان الکتریکی بیشتر از نمونه کنترل بود (05/0<p) و نیروی لازم برای فشردن دانه های انار تیمار شده تحت میدان الکتریکی طی انبارداری بیشتر از نمونه کنترل و نمونه های تیمار شده با میدان الکتریکی تنها در روز اول انبارداری بود. همچنین نتایج نشان داد که اعمال میدان الکتریکی توانست فعالیت آنتی اکسیدانی میوه انار را در حین انبارداری افزایش دهد.

    نتیجه گیری

    قرار گرفتن میوه در معرض میدان الکتریکی با ولتاژ بالا می تواند موجب تحریک تولید آنزیم های بازدارنده گونه های فعال اکسیژن در سیستم دفاعی میوه انار شود. علاوه بر این، این مطالعه اثربخشی تیمار میدان الکتریکی با ولتاژ بالا در نگهداری انار در مراحل پس از برداشت را ثابت کرد.

    کلید واژگان: انار, نگهداری, میدان الکتریکی ولتاژ بالا, ویژگی های کیفی}
    S Alipour, N Hamdami*, M Dalvi-Isfahan, S Fallah-Joshaqani
    Background and Objectives

    Several methods have been used to decrease losses during storage of fruits and vegetables. Of these methods, high-voltage electric field seems promising. The objective of this study was to investigate effects of high-voltage electric field on four major quality attributes of pomegranate fruit (Punica granatum L.) during storage.

     Materials & Methods

    Pomegranate fruits were exposed to high-voltage electric fields with strengths of 2 and 4 kV cm-1 at two various modes (use of high-voltage electric field alone at Day 1 of storage or repeat the high-voltage electric field treatment with the regular intervals during storage). Quality characteristics of the fruits such as color, texture, antioxidant content and respiration rate were assessed at 15-d intervals for 60 d.

    Results

    Results showed that the respiration rate of pomegranate fruits decreased with storage time extension and high-voltage electric field treated pomegranates demonstrated significantly lower respiration rates, compared to the control. The lightness value (L*) of pomegranates treated under electric field was higher than that of the control sample
    (p < 0.05) and the force needed to compress the pomegranate seeds treated under the electric field during storage was more than those of the control and samples treated with electric field alone on Day 1 of storage. Results also showed that use of electric field could increase the antioxidant activity of pomegranate fruits during storage.

    Conclusion

    It can be concluded that exposure to high-voltage electric field could induce or stimulate production of reactive oxygen species inhibitory enzymes in pomegranate defense systems. In addition, this study verified efficacy of high-voltage electric field treatment in postharvest preservation of pomegranate.

    Keywords: Pomegranate, Storage, High voltage electric field, Quality attributes}
  • Amita R*, Arya AB, Sathyabhama S, Debasish Gupta
    Background and Aims

    Leukoreduction of blood components has reduced the incidence of transfusion-associated adverse events. Leucofiltration is the most effective method of leukoreduction. We encountered haemolysis in a series of leucofiltered units. This stressed our precious inventory, added to financial loss, increased our turn-around time to issue leucofiltered blood units, and placed doubts on the safety of our leucofiltered products. A systematic root cause analysis was done to identify the reason for haemolysis.

    Materials and Methods

    A total of 69 units were leucofiltered during the study period, of which 13 units showed lysis following filtration.

    Results

    This study warranted a review of our existing leucofiltration standard operating procedures to keep strict adherence to manufacturer instructions to ensure quality in the end product.

    Conclusions

    Among the leucofiltered units showing hemolysis, 62% were due to higher temperature, 23% due to increased time for filtration, and 15% due to increased holding time of red cell units.

    Keywords: Haemolysis, Leucofiltration, Packed red cells, Root cause analysis, Storage, Temperature}
  • Fatemeh Mousavi, Seyed Mansour Razavi, Parisa Shojaei *
    Introduction
    Medication errors (MEs) are a common explanation for iatrogenic adverse events. This study appraised the incidence of MEs and identified the error types in the hospitals affiliated to Azad University of Medical Sciences, located in Tehran, Iran.
    Materials and Methods
    This cross-sectional descriptive study was conducted on all patients admitted to the hospitals affiliated to Azad University of Medical Sciences. The data were collected by means of a drug safety checklist and then extracted and analyzed in SPSS software (version 22.0). Moreover, drug interaction was assessed based on the World Health Organization guidelines and Medscape application.
    Results
    The mean MEs incidence was obtained as 43.38±5.25. Moreover, the mean numbers of errors in prescribing drugs and antibiotics were 4.71±2.93 and 1.02±0.95, respectively. According to the results, drug interactions occurred in 12.3% of the medicines. In addition, ME showed a significant correlation with education status, ward type, admission type, hepatic failure, hepatic enzymes, patient weight (when on antibiotics), antibiotic effect, and patient age (65> years).
    Conclusion
    The results were indicative of a high prevalence of prescription errors. Moreover, it was found that most of MEs were made by doctors, mainly due to the lack of an electronic drug registration system. Therefore, physicians need to be educated on how to increase patient safety through drug safety.
    Keywords: Hospital, Medication error, management, prescription, Storage, Usage}
  • AmirMohammad Monsef, Azam Abbasi *, Samaneh Shaghaghian, Reza Sharafati Chaleshtori
    Background

    walnut production and consumption has shown a steady rise in both amount and value in recent years. According to the seasonal production of walnuts, the storage condition plays a great role in its quality.

    Methods

    In this cross-sectional study, effects of storage temperature and removing hard peels of walnut were studied on the shelf life of walnut over 6 months. The aflatoxin levels, lipid oxidation, antioxidant activity, and sensory evaluation were measured every 16 days (d).

    Results

    The aflatoxin reduction was statistically signifcant in all stored walnut samples kept in the fridge, freezer, and their hard shell at room temperature after 151 days. The highest lipid oxidation and the lowest antioxidant activity were observed at the 6th month in walnuts preservedat room temperature. Through the sensory evaluation and chemical tests, walnuts preserved at fridge had the highest acceptance among panelist and least chemical changes after 6 months.

    Conclusion

    the best storage condition for a long time is keeping peeled walnuts in the fridge.

    Keywords: Walnut, Storage, Shelf life, Temperature, Aflatoxin}
  • Shadi Kolahdoozan, Sadaf G. Sepanlou, Elaheh Shaker, Ameneh Shayanrad, Reza Malekzadeh, Shahin Merat*, Hossein Poustchi
    Background

    It is increasingly common to collect and store specimens for future unspecified research. However, the effects of prolonged storage on the stability and quality of analytes in serum have not been well investigated. We aimed to determine whether the stability of liver enzymes extracted from frozen bio-samples stored at the baseline is affected by storage conditions.

    Methods

    A total of four liver enzymes in the sera of 400 patients were examined following storage. After deter-mining the baseline measurements, the serum of each patient was aliquoted and stored at −70°C for three and six months, as well as one, two, and five years after collecting the original sample. The percent change from baseline measurements was calculated both statistically and clinically. Linear models were also used to correct the results of the samples based on the time they were frozen.

    Results

    In almost all samples, liver enzymes were detectable until two years after the baseline, while in a signifi-cant proportion of samples, enzymes were not ultimately detectable five years after the baseline. Linear regression analysis on log-transformed levels of enzymes shows that the performance is acceptable until one year after the baseline. The performance of the prediction model declines substantially two and five years after the baseline, except for GGT.

    Conclusion

    Long-term storage of serum samples significantly decreases the concentration of the liver enzymes from the baseline, except for GGT. It is not recommended to store samples for more than two years, as liver en-zymes are not detectable afterwards

    Keywords: Enzymes, Liver, Storage, Temperature, Time}
  • Ali Dini *
    Introduction

    Pistachio nuts are considered a functional foodstuff containing various antioxidants consumed mostly in a roasted form. Mild to severe roasting conditions can cause oil oxidation. Therefore, different methods, such as coating, are used to increase the shelf life of nuts.

    Materials and Methods

    Tragacanth-based edible coatings were prepared using propylene glycol as a plasticizer both individually and in combination with free α tocopherol (FT), or its Nanocapsules (NC) and Nanoesphers (NS). Chemical and sensory properties of coated pistachio nuts were measured during 6-month storage at 40, 50, and 60ᵒC.

    Results

    At storage temperatures of 40, 50, and 60°C, PV and Pa.V were evaluated 3.92, 3.85, 7.1 and 6.89, 13.3, 21.1 meq/kg, respectively. Unlike acidic value, coating with tragacanth caused a significant reduction in PV and Pa.V indices as they did not exceed 4 and 6, respectively. Sensory evaluation showed the overall acceptance of uncoated roasted pistachios to be lower than average after 120 and 60 days of storage at 50 and 60°C, respectively. Thus, the anisidine value was measured in the range of 2.9-5.6. No significant difference was observed in oxidation indices and sensory properties of coated samples with different forms of α-tocopherol.

    Conclusion

    Using nanoparticle forms of α-tocopherol in coating reduced the oxidation rate and improved overall acceptance during storage. There was a correlation between the chemical markers of oxidation and sensory properties of roasted pistachio nuts, and p-anisidine value was appropriate to monitor pistachio nuts' oxidative during shelf-life.

    Keywords: edible coating, Pistachio Nuts, α-Tocopherol, Nanocapsule, Spontaneous Emulsification, Storage}
  • Ahmad Shakerardekani *, Mohamad Sadeghi
    Introduction

    Drying is one of the most important stages of pistachio processing. The aim of this study was to investigate two common types of dryers available in Rafsanjan with emphasis on health related aspects. Fresh pistachio cultivars of Fandoghi were harvested from Rafsanjan gardens and the pistachio samples were dried after peeling and washing using Silage and Wagon dryers.

    Materials and Methods

    After drying, pistachio cultivars of Fandoghi were tested for 0, 1.5 and 3 months at room temperature (20 ± 2 ºC). Moisture content, peroxide value, conjugated dienes and conjugated triene, mold and yeast count were measured with three replicates. The experiment was conducted as a factorial experiment in a completely randomized design with three replications.

    Results

    Peroxide value of dried pistachio samples in two drying machines were in the permissible range (less than 1 millequivalent /kilogram). Silage dryer samples showed less peroxide value, conjugated dienes and conjugated trienes than wagon dryer samples. At the end of the three-month period, the growth rate of mold and yeast in pistachio samples dried using wagon dryer decreased and their growth rate was less than the permissible limit (less than 1000 cell forming unit/gram).

    Conclusion

    Mold and yeast growth were not observed in dried pistachio samples using silage dryer. It is recommended to use more silage dryer in pistachio processing units.

    Keywords: Pistachio nut, dehydration, Sensory, Health, Storage}
  • قربان زارع گشتی*، یاسمن اعتمادیان، علی اصغر خانی پور، ژاله خوشخو، صغری کمالی
    سابقه و هدف
    با توجه به کاهش ذخایر آبزیان در چند سال اخیر، استفاده از منابع جدید پروتئینی گسترش یافته که بهره برداری از میگوهای ریزجثه تالاب بین المللی بندر انزلی به دلیل ارزش غذایی بالا، ضروری به نظر می رسد.
    مواد و روش ها
    میگوهای غیربومی Macrobrachium nipponense پس از صید و شستشو با آب سرد، در دستگاه های آتموس، آون معمولی و آون خلاء در دمای C◦70 به مدت 6، 21 و 7 ساعت خشک گردیده؛ و سپس سر و پوسته آن ها جدا و گوشت خشک شده در فیلم های پلی اتیلنی با پوشش آلومینیومی تحت شرایط خلاء، بسته بندی و به مدت 6 ماه در دمای محیط نگهداری و مورد ارزیابی قرار گرفت.
    یافته ها
    نتایج نشان داد که گوشت خشک میگو با مقادیر بالایی از پروتئین و اسیدهای چرب امگا 3، قابلیت ماندگاری به مدت 6 ماه در دمای محیط را دارد. همچنین نتایج حاصل از مقایسه خشک کردن میگو در سه دستگاه ذکر شده نشان داد که به ترتیب کیفیت نهایی فرآورده در دستگاه آون خلاء و کنترل رطوبت در دستگاه آتموس بهتر بوده است. از سویی دیگر، تغییرات اکسیداسیون چربی تا ماه ششم در تمام تیمارها به خوبی کنترل شد و نیز شمارش میکروارگانیسم ها در میگوهای خشک شده به وسیله دستگاه آتموس نسبت به آون معمولی و خلاء، کمتر بود. در رابطه با ارزیابی حسی تیمارها، امتیاز 5 (عالی) برای ماه اول و امتیاز 3 (خوب) در پایان مدت زمان نگهداری، شاخص اعلام گردید.
    نتیجه گیری
    گوشت خشک حاصل از میگوهای ریز M. nipponense به دلیل پروتئین بالا و اسیدهای چرب چند غیر اشباع می تواند به عنوان یکی از تنقلات مغذی در جامعه معرفی و مورد استفاده قرار گیرد.
    کلید واژگان: تالاب بندر انزلی, میگوی ریز M, nipponense, خشک کردن, تغییرات کیفی, نگهداری}
    Ghorban Zarehgashti*, Yasaman Etemadian, Aliasghar Khanipour, Zh Khoshkhoo, S Kamali
    Background and Objectives
    With recent declines in aquaculture resources, the use of new protein sources has expanded; as a result, it is necessary to use tiny shrimp of Anzali International Lagoon  for its high nutritional value.
    Materials & Methods
    Macrobrachium nipponense non-native shrimp, after catching and washing with cold water, was dried in cabinet, oven and under vacuum dryers at 70°C for 6, 21 and 7 hours. Then the shells and heads were removed. Finally, the dried meats were packed in metalize films under vacuum and evaluated at room temperature for 6 months.
    Results
    It was shown that the dried shrimp meat with high protein and omega-3 was saved at room temperature for 6 months. Also the results of shrimp drying in the three mentioned devices indicated that the final quality of the product and moisture control in vacuum oven and cabinet devices were better, respectively. On the other hand, lipid oxidation changes to six months were well controlled in all treatments, and the count of microorganisms in the shrimp dried by the cabinet dryer was less than that of the ordinary oven and vacuum. In relation to sensory evaluation of the treatments, the score of 5 (excellent) for the first month and the score of 3 (good) at the end of the maintenance period were announced.
    Conclusion
    Dried meat from M. nipponense tiny shrimp can be introduced and used as a nutritious snack in the community due to its high protein and unsaturated fatty acids.
    Keywords: Bandar Anzali lagoon, M. nipponense tiny shrimp, Drying, Qualitative variations, Storage}
  • Ahmad Shakerardekani *, Abdolhossein Mohamadi
    Introduction

    Roasting process improve the taste, colour, texture, and overall acceptability of the tree nuts.

    Materials and Methods

    In this study, raw pistachio were placed in a solution containing 10, 15, 20 and 25% salt for 15 min to select suitable conditions of salting and roasting. Then pistachio roasted in oven and fluidized bed roaster for 10-25 min (130-190˚C). Sensory evaluation was carried out in terms of frangibility, salt percent, oil rancidity, flavor, color of shell, splitting of shell and overall acceptability. Salted and roasted pistachio samples stored at 25˚C for one year. Peroxide value, acid value, amount of salt and moisture content was measured each two months during storage period.

    Results

    The pistachio salted in 10% salt solution and roasted in fluidized bed roaster at 130˚C (30 min), 190˚C (10 min) and 190˚C (30 min) obtained highest score respectively. Quality test results showed that salted and roasted pistachios maintain its quality up to 10 months.

    Conclusion

    The best condition for salting and roasting of pistachio was salting in 10% salt solution and roasting at 130˚C for 30 min in fluidized bed roaster.

    Keywords: Salting, Toast, Storage, Sensory, pistachios}
  • Fabrice Djikeng *, Audrey Therese Nzwinda, Mallampali Karuna, Hilaire Womeni
    The effect of sunlight on the quality of groundnut oil in comparison with the same oil samples stored in the dark at room temperature was aimed to study. The oil samples were exposed to sunlight (34-40°C) and ambient storage (~24°C in the dark) for 90 days. The samples were collected every 30 days for the analysis of their physicochemical properties including peroxide value (PV), p-anisidine (p-AnV), total oxidation (TOTOX), thiobarbituric acid (TBA), free fatty acid (FFA), color, induction period on Rancimat and fatty acid composition. At the beginning of the experiment, the PV, p-AnV, TOTOX, TBA and FFA values of groundnut oil were 10.31 meq O2/kg, 4.39, 25.01, 0.99 and 1.49% respectively; color 1.40R, 22.00Y and induction time (IT) 5.33 h. After 90 days storage at room temperature, the PV, p-An-V, TOTOX, TBA and FFA values were respectively ranged between 10.31-21.32 meq O2/kg, 4.39-7.36, 25.01-50.12, 0.99-2.42 ppm and 1.49-2.64%; the color in the red and yellow unit 1.40-1.20R and 22.00-19.00Y respectively; and the induction time 5.33-3.77 h. However, the same parameters at the end of exposure to sunlight were ranged in the same order, between 10.31-43.47 meq O2/kg, 4.39-22.24, 25.01-109.18, 0.99-3.25 ppm and 1.49-5.83% respectively; the color in the red and yellow units 1.40-0.50R and 22.00-1.30Y respectively; and the induction time 5.33-0.03 h. Results also showed that the amount of linoleic and gadoleic acids significantly dropped under sunlight compared to dark at room temperature. Sunlight significantly reduced the quality of groundnut oil. This oil should be stored in the dark at room temperature.
    Keywords: Groundnut oil, Sunlight, Physicochemical property, Storage}
  • Shideh Montasser Kouhsari *, Ehsan Gharib
    Pancreatic β-cells dysfunction and impairment of insulin action usually leads to hyperglycemia. Punica granatum L. is a well-known traditional herbal remedy in Iran due to its positive effects on ameliorating blood glucose homeostasis. In this study, we aimed to investigate and compare the pomegranate fruits aqueous extract (PE) on glucose and lipid metabolisms in normal and Alloxan-induced diabetic male Wistar rats. Therefore, normal (N) and diabetic (D) rats were each divided into four groups (control, a, b and c) which were treated orally with PE at doses of 0, 100, 200 and 350 mg/kg (bw), respectively. Oral glucose tolerance test (OGTT) followed by short-term and long-term glycemic controls were performed on the experimental groups. Also, plasma insulin, free fatty acids and triglyceride levels and tissues contents of glycogen and triglyceride were evaluated. By using Real-time PCR, the possibility of modulations of the Insulin receptor substrate 1 (IRS-1), Protein kinase B (Akt), Glucose transporter 2 and 4 (Glut-2, 4) mRNAs expression levels in treated rats were investigated. The obtained data showed noticeable reduction in fasting blood glucose (FBG) by 28.1% and 67.9% in short-term and long-term treatment models, respectively, in the PE+Dc group. Also, there were marked increase in the mRNAs expression levels of IRS-1, Akt, Glut-2 and Glut-4, which results in improvement of glucose uptake and storage. Taking together, it is suggested that PE administration contributed to the modulation of both hyperglycemia and hyperlipidemia in Alloxan-diabetic Wistar rats.
    Keywords: Alloxan monohydrate, Diabetes mellitus, Insulin expression, secretion, Insulin signaling mediators, Glucose uptake, storage, Pomegranate fruits aqueous extract}
  • محمدرضا دهقانی مشکانی، حسنعلی نقدی بادی*، کامبیز لاریجانی، علی مهرآفرین
    مقدمه
    عملیات پس از برداشت نظیر عملیات خشک کردن و شرایط انبارداری تاثیر زیادی بر کمیت و کیفیت مواد مؤثره گیاهان دارویی دارد.
    هدف
    بررسی اثر پیش خشک کردن و شرایط و زمان انبارداری بر میزان و اجزای اسانس گیاه دارویی آویشن دنایی از اهداف این تحقیق می باشد.
    روش بررسی
    این آزمایش به صورت فاکتوریل بر پایه طرح بلوک های کامل تصادفی در سه تکرار اجرا شد. فاکتور اول شامل عملیات پیش خشک کردن (شامل پیش خشک کردن و عدم پیش خشک کردن) ، فاکتور دوم شامل دو روش انبارداری (نگهداری در سایه و نگهداری در یخچال با دمای 4 درجه سانتی گراد) و فاکتور سوم شامل مدت زمان انبارداری (نگهداری اندام خشک گیاه به مدت 60، 120 و 180 روز) می باشد. جهت استخراج اسانس، از دستگاه کلونجر و برای شناسایی ترکیبات تشکیل دهنده اسانس از دستگاه GC/Mass و دستگاه GC استفاده شد.
    نتایج: بیشترین مقادیر اسانس و مواد مؤثره لینالول، بورنئول، ترپینن-4 ال، تیمول متیل اتر، ای- انتول، تیمول و کارواکرول در زمان نگهداری 60 روز مشاهده شد. همچنین بیشترین مقادیر آلفا- پینن، کامفن، بتا- پینن، میرسن، آلفا فلاندرن، آلفا-ترپینن، لیمونن، سیس- سابینن هیدرات و ترانس سابینن هیدرات در گیاهان خشک شده در سایه بعد از عملیات پیش خشک و نگهداری در یخچال به مدت 120 روز مشاهده شد.
    نتیجه گیری
    خشک کردن گیاه آویشن دنایی در سایه بدون اعمال پیش خشک و نگهداری آنها در دمای 4 درجه سانتی گراد بهترین روش برای حصول حداکثر میزان اسانس و ترکیبات اصلی آن (تیمول و کارواکرول) بود.
    کلید واژگان: آویشن دنایی, اسانس, انبارداری, تیمول, عملیات خشک کردن, کارواکرول}
    MR Dehghani Mashkani, H Naghdi Badi*, K Larijani, A Mehrafarin
    Background
    The post-harvesting process of medicinal plants such as drying operation and storage conditions has great influence on their quality and quantity of the active ingredients.
    Objective
    The aim of this experiment was to investigate the effect of pre-drying operation and also storage conditions and duration on the essential oil content and composition of Thymus daenensis.
    Methods
    A factorial experiment was conducted based on randomized complete block design with three replications. The first factor include drying operations (including pre-drying and without pre-drying), the second factor include storage method (shade storage and storage in a refrigerator at 4 °C), and the third factor was storage duration (storing the plant’s organs for 60, 120 and 180 days). The essential oil was extracted by clevenger apparatus and its components were identified using GC and GC/Mass.
    Results
    The highest amounts of essential oil, linalool, borneol, terpinene-4-ol, thymol methyl ether, e-anethol, thymol and carvacrol were observed in plants stored for 60 days. Also, the highest amounts of α-pinene, camphen, β-pinene, myrcene, α-phellandrene, α-terpinene, limonene, cis-sabinene hydrate and trans-sabinene hydrate were observed in the plants dried in the shade after pre-drying operation and stored in the refrigerator for 120 days.
    Conclusion
    Drying of T. daenensis plants under shade without pre-drying operation and storing in 4 ° C was the best way to obtain the highest amounts of essential oil and its main components (thymol and carvacrol).
    Keywords: Thymus daenensis Celak., Carvacrol, Drying operation, Essential oil, Storage, Thymol}
  • مینا پنجی، پیمان قجربیگی، سعید شهسواری، رزاق محمودی *
    زمینه وهدف
    توت فرنگی محصولی ارزشمند بوده که عمر انباری نسبتا کوتاهی دارد. کاربرد ترکیبات شیمیایی غیرطبیعی ضد قارچ جهت افزایش ماندگاری این میوه نگرانی هایی به دنبال داشته است. در سال های اخیر استفاده از پوشش های خوراکی به عنوان یک روش ایمن گسترش یافته که تا حد زیادی در حال جایگزینی با موارد سنتزی هستند. هدف این مطالعه افزایش ماندگاری و حفظ کیفیت میوه توت فرنگی با استفاده از پوشش خوراکی کنسانتره پروتئین آب پنیر و اسانس دانه زنیان طی 10 روز انبار مانی در دمای 1± 4 درجه سلسیوس و رطوبت نسبی 5± 75 درصد بود.
    روش بررسی
    در مطالعه ای آزمایشگاهی، اسانس دانه زنیان با دستگاه کلونجر، استخراج و با دستگاه گاز کروماتوگراف – طیف سنج جرمی ، اجزای تشکیل دهنده آن شناسایی شد. در این تحقیق از پوشش خوراکی بر پایه کنسانتره پروتئین آب پنیر و اسانس دانه زنیان در غلظت های متفاوت (2/0، 4/0 و 6/0 درصد) استفاده شد. کیفیت میکروبی (تعداد کپک ومخمر و بارمیکروبی کل) ، خصوصیات فیزیکوشیمیایی (کاهش وزن، اسیدیته، pH و کل موادجامد محلول، سفتی بافت و پوسیدگی) و ویژگی های حسی توت فرنگی های پوشش داده شده پس از بسته بندی، قبل از انبارمانی (لحظه صفر) و سپس 3، 5، 8 و 10 روز پس از انبار مانی در مقایسه با نمونه کنترل ارزیابی گردید.
    یافته ها
    این پوشش به طور معنی داری رشد میکروارگانیسم ها را به تاخیر انداخت به طوری که با افزایش غلظت 4/0 و 6/0 درصد اسانس میزان کپک و مخمر در طی 10 روز انبارمانی، به صفر رسید و بار میکروبی کل یک سیکل لگاریتمی کاهش یافت. در مقایسه با نمونه شاهد استحکام بافت افزایش ، افت وزن، و درصد پوسیدگی به طور معنی داری کاهش یافت. در روز دهم میزان پوسیدگی برای گروه کنترل 86% و برای تیمارحاوی 6/0 درصد اسانس، 10% بود. تغییرات pH، اسیدیته و مواد جامد محلول نامحسوس بود. بیش ترین امتیاز ویژگی های حسی مربوط به تیمار با 2/0 درصد اسانس بود که این تیمار همچنین تاثیر مثبتی بر پارامترهای مورد مطالعه داشت.
    نتیجه گیری
    پوشش دهی میوه توت فرنگی با کنسانتره پروتئین آب پنیر و اسانس دانه زنیان می تواند به عنوان روشی ایمن و کارا در افزایش نگه داری و حفظ بهتر کیفیت میوه توت فرنگی در شرایط سرد معرفی شود.
    کلید واژگان: توت فرنگی, پوشش خوراکی, انبارمانی, پروتئین آب پنیر, اسانس دانه زنیان}
    Razzagh Mahmoudi*
    Background and Aim
    Strawberry is a valuable product that has a relatively short storage life. Use of chemical fungicides to extend storage life has been a matter of concern. In recent years, use of edible coatings has been regarded as a safe method in order to replace synthetic coatings. The aim of this study was to increase the storage-life and maintain the quality of strawberry by using edible coatings of whey protein concentrate and Trachyspermum copticum essential oil during a storage time of 10 days at 4 ± 1 ° C and relative humidity of 75 ± 5%.
    Materials and Methods
    We extracted the essence of the seeds by Clevenger apparatus. Then, the active components of the essence were identified by gas chromatography - mass spectrometry (GC-MS) method. In this study we used edible coating of whey protein concentrate and Trachyspermum copticum essential oil at different concentrations (0.2, 0.4 and 0.6%).Microbial quality )number of molds and yeasts, total microbial count(, physicochemical characteristics (weight loss, acidity, pH, soluble solids, texture firmness, and decay) and organoleptic characteristics were assessed in the experimental group after packing, before storage (at the beginning of storage ) and then 3, 5, 8 and 10 days after storage and compared with those in the control group.
    Results
    This coating significantly delayed the growth of microorganisms. By increasing concentrations of 0.4 and 0.6%, the mold and yeast counts after 10 days of storage reached zero and we found a logarithmical cycle reduction in the microbial load. As well texture firmness, weight loss, and decay percentage significantly decreased in the experimental group compared to those in the control group. On the 10th day, the decay rate was 86% for the control group and 10% for the case group containing 0.6% essential oil. Changes in PH, acidity and soluble solids were not significant. The best organoleptic properties were related to the priming with 0.2% essential oil, which also had a positive effect on the parameters in our study.
    Conclusion
    Strawberry fruit coating with whey protein concentrate and Trachyspermum copticum essential oil can be regarded as a safe and effective way to increase the storage life and quality of strawberry fruit in cold environments
    Keywords: Strawberry, edible coating, Storage, whey protein, Trachyspermum copticum essential oil}
  • بهادر حاجی محمدی، هاشم منتصری، سلیمان ارجمند طلب*، محمد مهدی رزمجو، محراب صیادی، محمد جلالی، مهرنوش شیردلی
    مقدمه
    تحقیقات بیانگر افزایش مصرف محصولات پروبیوتیک در جهان است. میزان بقا باکتری های پروبیوتیک و ویژگی های حسی از مهمترین فاکتورهای کیفی ماست پروبیوتیک به شمار می آیدکه تحت تاثیر عوامل مختلفی قرار می گیرند. بنابراین هدف این مطالعه بررسی زنده مانی باکتری های پروبیوتیک و خواص حسی دو نوع ماست پروبیوتیک بود.
    روش بررسی
    با استفاده از استارترهای تجاری ABY3 و MY 1821 دو نوع ماست پروبیوتیک تهیه گردید. به وسیله ی روش پورپلیت تعداد باکتری های لاکتوباسیلوس اسیدوفیلوس، بیفیدوباکتر لاکتیس و لاکتوباسیلوس کازیی و ویژگی های حسی شامل طعم، احساس دهانی، ظاهر و بافت هر دو نوع ماست در مدت 21 روز و با فاصله زمانی هفت روزه اندازه گیری شد. نتایج به دست آمده با استفاده از آزمون های آماری T-test و آنالیز واریانس با اندازه های تکراری در نرم افزار SPSS نسخه 18 با سطح معنی داری 05/0 تحلیل گردید.
    یافته ها
    در پایان تعداد باکتری های لاکتوباسیلوس اسیدوفیلوس و بیفیدوباکتری در ماست MY 1821 به ترتیب 04/0 ± 24/7 و 06/0 ± 90/6 و در ماست ABY3 11/0 ± 45/6 و 06/0 ± 90/6 کلنی در گرم (Cfu/gr) بود. تعداد باکتری لاکتوباسیلوس کازیی در ماستMY-1821 04/0 ±7. 55 تعیین گردید. همچنین تعداد لاکتوباسیلوس اسیدوفیلوس به میزان 1/1 و 89/0Cfu/gr، بیفیدوباکتری به میزان 48/0 و 38/1 Cfu/gr در ماست MY 1821 و ABY3 کاهش نشان دادند، تعداد لاکتوباسیلوس کازیی نیز به میزان 55/0 در ماستMY 1821 کاهش یافت. متوسط امتیاز طعم، احساس دهانی، بافت و ظاهر در ماست MY 1821 به ترتیب 09/3 ± 2/21، 80/1 ± 36/12، 014/1 ± 2/7 و 352/0 ± 86/3 و در ماست ABY3 به ترتیب 22/4 ± 6/17، 46/2 ± 73/10، 97/0 ± 66/6 و 488/0 ± 33/3 به دست آمد.
    نتیجه گیری
    تعداد باکتری های پروبیوتیک در پایان مطالعه بالاتر از محدوده استاندارد ملی ایران بود. همچنین ویژگی های حسی هر دو نوع ماست تا پایان مطالعه رضایت بخش تا بسیار رضایت بخش ارزیابی گردید. لذا این استارترها در صنایع لبنی جهت ارتقا سلامت عمومی جامعه توصیه می گردد.
    کلید واژگان: باکتری های پروبیوتیک, ماست, ارزیابی حسی, زنده مانی, نگهداری}
    Bahador Hajimohammadi, Hashem Montaseri, Soleyman Arjmandtalab*, Mohammad Mahdi Razmjoo, Mehrab Sayadi, Mohammad Jalali, Mehrnosh Shirdeli
    Introduction
    Because of many health benefits, production and cosumption of probiotic products was incresead worldwide. Viability of probiotic bacteria and sensory propperties are important factors in quality of probiotic yoghurt. Many factor can affect the sensory property and survival of probiotic bacteric in yoghurt. Thus, the aim of this study was to assess the the viability of probiotic bacteria and sensory properties of two kind probiotic yoghurt.
    Methods
    Two kind of probiotic yoghurt named as MY 1821 and ABY3 were made using commercial starter culures according to manufacturer instruction. Viability of probiotic bacteria (lactobacillus acidophilus, bifidibacteria and lactobacillis casei) and sensory properties (flavour, mouthfeel, appearance and texture) of yoghut were assessed during 21-days at 7 days interval. statistical analysis was carreid out using T-test and ANOVA (repeated measure) in SPSS software. P value less than 0.05 was considered as significant.
    Results
    At the end of experiments the number of lactobacillus acidophilus and bifidobacteria were 7.42± 0.04 and 6.90 ± 0.06 log cfu/gr in MY1821 and 6.45 ± 0.11 and 6.16 in ABY3 and the number of lactobacillis casei was 7.55± 0.04 log cfu/gr in MY 1821. population of lactobacillus acidophilus and bifidobacteria decreased by 1.1 and 0.48 log cfu/gr in MY 1821 And 0.89 and 1.38 cfu/gr in ABY3 yoghurts.viability of L. casei also reduced and by 0.55 Log cfu/gr in MY 1821 yoghurt. At the end of experiments the mean score for flavour, mouthfeel, appearance and texture were 21.2± 3.09 , 12.36 ± 1.80, 7.2± 1.1.01, 3.86± 0.35 in MY 1821 yoghurt and 17.6±4.22, 10.73±2.46, 6.66± 0.97 and 3.33± 0.49 in ABY3 yoghurt respectively.
    Conclusion
    The number of probiotic bacteria at the end of storage were above the criteria determined by Iran national standard. The result of sensory evaluation showed that the prepared yoghurt had good to excellent acceptance. Thus using these starter cultures by diary industry is recommended. Because of helth benefits of probiotics, consomption of these products for their high population of probiotic bacteria can improve the public health.
    Keywords: probiotic bacteria, yoghurt, sensory test, viability, storage}
  • Z. Salehi, M. Shams, Ghahfarokhi *, A. Fattahi, M. Ghazanfari, S.A. Yazdanparast
    Aims
    Transportation of clinical samples and long-term recoverability of fungal strains are critical to epidemiological studies. In addition, the study of fungi often requires the use of living pure cultures. The aim of this study was to evaluate the methods used to preserve culture collections of dermatophytes, consisted of storage in sterile distilled water, cryopreservation with glycerol, preserving in tryptic soy broth (TSB), and freezing mycobiotic agar.
    Materials and Methods
    in this experimental study, ninety-two dermatophyte isolates belonged to 10 species were tested. The freezing protocol was done in 4 forms of sterile distilled water, cryopreservation with glycerol, freezing mycobiotic agar, and preserving in TSB. The viability of the dermatophytes species was assessed after 3 years at morphological (macro and microscopic features), physiological (Using Dermatophyte Test Medium; DTM, urease test media, and the hair perforation test), and genetic levels by restriction fragment length polymorphism (RFLP).
    Findings
    The survival rate was 84 out of 92 water stored fungal strains (91.3%) and 81 out of 92 mycobiotic agar stored strains (88.0%) and 75 out of 92 glycerol 40% stored strains (81.5%) and 43 out of 92 TSB stored fungal strains (46.7%). Overall, more than 88% of the strains survived in the distilled water storage and freezing mycobiotic agar, methods, while storage in TSB had the least success in the maintenance of dermatophytes.
    Conclusion
    The procedure to preserve cultures in sterile distilled water is reliable, simple, and inexpensive.
    Keywords: Dermatophytes, Storage, distilled water, freezing mycobiotic agar, TSB, Cryopreservation PCR, RFLP}
  • لیلا فهمیده*، محمود توکلی*، سمیرا عمرانی پور، امیر رجبی
    سابقه و هدف
    وضعیت ظاهری محصولات مهم ترین شاخص ارزیابی بازارپسندی است. وجود هر گونه علایم آلودگی، پوسیدگی و نرم شدن میوه طی انبارداری باعث کاهش بازارپسندی محصول می شود. به همین دلیل استفاده از روش های ایمن برای کنترل فساد و حفظ کیفیت بافت میوه در دمای مناسب در انبار امری ضروری است.
    مواد و روش ها
    به منظور ارزیابی اثرات تیمارهای مواد شیمیایی و دما بر روی ویژگی های کمی و کیفی میوه چیکو رقم اوال در مرحله پس از برداشت، آزمایشی در قالب فاکتوریل بر پایه طرح کاملا تصادفی در مرکز تحقیقات کشاورزی شهرستان جیرفت انجام گرفت. در این پژوهش عوامل مورد بررسی شامل مواد شیمیایی (کلریدکلسیم 60 میلی مولار و اسپرمیدین 2 میلی مولار) و دما (7 و 12 و 22 درجه سانتی گراد) بود.
    یافته ها
    نتایج جدول تجزیه واریانس نشان داد که اکثر صفات مورد بررسی در محدوده ارقام معنی دار هستند. تیمار کلریدکلسیم و نگهداری در دمای 7 درجه سانتی گراد نقش موثرتری را نسبت به سایر تیمارها بر روی ویژگی های افت وزن، حفظ سفتی بافت، حفظ میزان pH، مقدار ماده جامد محلول، اسیدیته قابل تیتراسیون و رنگ نشان داد. تیمار شاهد و نگهداری در دمای 22 درجه، زودتر باعث فساد، خرابی و تغییرات شیمیایی میوه شد.
    نتیجه گیری
    عمر انبارمانی با کلرید کلسیم با نگهداری در دمای 7 درجه سانتی گراد می تواند به عنوان روشی ایمن و کارا در افزایش نگه داری میوه چیکو در انبار و حفظ بهتر کیفیت این میوه ی سلامتی بخش معرفی شود.
    کلید واژگان: چیکو, کلرید کلسیم, اسپرمیدین, انبارمانی, کیفیت}
    L. Fahmideh, M. Tavakoli *, S. Omranipour, A. Rajabi
    Background And Objectives
    One of the most important factors of consumer acceptance is the products appearance. Any signs of contamination, caries and softening of fruits during storage, leads to a reduction in market acceptance of the products. So, it is essential to use safe controling methods to prevent the damage and maintain the quality of fruit at proper warehouse temperatures.
    Materials and Methods
    In order to evaluate the effects of preservative and temperature treatments on quantitative and qualitative characteristics of Chico-Oval- fruit at post-harvest stage. A randomised controlled trial with full factorial designs study was conducted at Jiroft Agricultural Research Center. In this study two factors included preservative (calcium chloride 60 mM and spermidine 2 mM) and temperature (7, 12 and 22°C) were studied.
    Results
    The analysis of variance showed that most of the studied factors were statistically significant. The treatments of calcium chloride and storage at 7°C were more effective than other treatments on factors like wieght loss, Soft-Tissue Firmness, pH, soluble solids content, titratable acidity and color. Control and treatment at a temperature of 22°C showed earlier damage, deterioration and chemical changes in the Chico fruit.
    Conclusion
    Calcium chloride and storage at 7°C can increase the storage life of Chico fruit in warehouses.
    Keywords: Chieko, CaCl2, Espermidine, Storage, Quality}
  • ابراهیم یارمحمدی، زهرا خاموردی، فرناز منصف اصفهانی *، علیرضا سلطانیان
    پیش زمینه و هدف
    استفاده از آنتی اکسیدان ها می تواند بر ماندگاری استحکام باند سیستم های ادهزیو تاثیر داشته باشد. هدف این مطالعه بررسی دو ادهزیو اچ و شستشوی دومرحله ای با حلال های متفاوت بر دوام باند کامپوزیت رزین به عاج درمان شده با پروآنتوسیانیدین بود.
    مواد و روش کار
    80 دندان مولر سالم، بعد از برداشتن مینای سطح اکلوزال با دیسک الماسی، بر اساس نوع حلال موجود در ادهزیو به طور تصادفی به 4 گروه تقسیم شدند، که در هر گروه نیز نیمی از نمونه ها تحت درمان سطحی با پروانتوسیانیدین قرار گرفته و نیمی دیگر به عنوان گروه کنترل در نظر گرفته شدند. بعد از گذشت 24 ساعت پس از آماده شدن نمونه ها طبق دستور کارخانه سازنده و نگهداری در آب مقطر، تحت ترموسیکل و برش قرار گرفتند، نیمی از نمونه ها بلافاصله و نیمی دیگر 6 ماه بعد با دستگاه استحکام باند ریز کششی، مورد آزمایش قرار گرفتند. نحوه شکست نمونه ها با استریو میکروسکوپ و مورفولوژی سطح شکست با SEM بررسی شد.
    یافته ها
    هیچ یک از متغیرهای کاربرد پروآنتوسیانیدین، نوع حلال، و ذخیره سازی به تنهایی اثر معنی داری را بر مقادیر استحکام باند ریز کششی نمونه ها نشان ندادند، ولی در بررسی اثر متقابل کاربرد پروآنتوسیانیدین و نوع حلال، و نیز اثر متقابل کاربرد پروآنتوسیانیدین و ذخیره سازی، تاثیر معنی دار مشاهده گردید (05/0>P).
    بحث و نتیجه گیری
    کاربرد پروآنتوسیانیدین باعث ثبات باند می گردد و همراه با ادهزیو هایی با پایه الکلی، این اثر افزایش پیدا می کند.
    کلید واژگان: پروآنتوسیانیدین, استحکام باند, باندینگ, دخیره سازی}
    Ebrahim Yarmohammadi, Zahra Khamverdi, Farnaz Monsef Esfehani *, Alireza Soltanian
    Background and Aims
    Use of the antioxidants can affect the durability of bond strength in some adhesive systems. This study sought to evaluate the effect of two types of the two step etch and rinse adhesive systems with different solvents on durability of resin composite bonding to the treated dentin with proanthocyanidins.
    Materials and Methods
    In this in vitro experimental study, 80 human molar teeth, after removal of the coronal enamel, were randomly divided into 4 groups, based on the type of solvent used in the adhesive. Half of them were treated with proanthocyanidins, and others were considered as the control. Thermocycling and cutting were done on the specimens 24 hours after preparing and water storage. Half of them were immediately subjected to the microtensile bond strength test, and the others after 6 months storage in distilled water. The mode of failures and the fractured surface morphology were examind under a stereomicroscope and scanning electron microscope, respectively.
    Results
    None of the variables including the use of proanthocyanidin, type of solvent and storage had significant effect lonely, but the interaction between the use of proanthocyanidin and solvent type, and the interaction between the use of proanthocyanidin and storage had statistically significant effect on the values of microtensile bond strenght (P
    Conclusion
    Use of proanthocyanidin leads to bond stability and this effect improves significantly accompanying adhesives with ethanolic base.
    Keywords: proanthocyanidins, Bond strength, Adhesive, Storage}
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