جستجوی مقالات مرتبط با کلیدواژه "white adipose tissue" در نشریات گروه "پزشکی"

Beta-adrenergic signaling deficiency has been established to be related to obesity and related diseases. Beta3- adrenergic receptor (Adrb3) and beta-arrestin2 (Barr2) are pivotal agents in the beta-adrenergic-signaling pathway. This study aimed to investigate the preventive effect of aerobic training on dysregulation of Adrb3 and Barr2 gene expression that was induced by high-fat diet (HFD) in inguinal white adipose tissue of mice.

Twenty-one C57BL/6 mice were assigned to three groups as follows: 1) control group (n=7), 2) high-fat diet- induced overweight (HFD-OW) (n=7), and 3) high-fat diet with aerobic training (HFD-AT) (n=7). The HFD-OW group were fed with a HFD for 12 weeks. The HFD-AT group had aerobic training for six weeks on a treadmill in addition to feeding with the HFD. The real-time polymerase chain reaction (PCR) method was used to measure the gene expression of Adrb3 and Barr2 in inguinal white adipose tissue.

The gene expression of Adrb3 did not significantly change between groups (P > 0.05). However, the expression of Barr2 in HFD- OW group was significantly increased as compared to the control group (1.5-fold: P = 0.001). Interestingly, the Barr2 expression in HFD-AT group was significantly lower compared with HFD-OW group (P = 0.045).

The results indicated that aerobic training could inhibit the upregulation of Barr2 induced by HFD. It seems that a portion of the preventive effect of aerobic training on the development of obesity may be mediated by inhibiting the Barr2 expression in adipose tissue.

Reducing white adipose tissue is an important strategy for treatment of obesity and its related metabolic disorders. The purpose of this study was to determine the effect of endurance exercise and adenosine consumption on the expression of selected genes of thermogenic pathway in the visceral adipose tissue of obese male rats. Twenty male wistar rats were fed on a high-fat diet (40 % fat) 12 weeks to get mean weight 319±30 gr. The rats were selected randomly in four groups; control-saline, control-adenosine, exercise-adenosine and exercise-saline. The adenosine groups received 0.2mgr/kg adenosine 12 weeks (seven days a week), 3 hours before exercise. The exercise schedule included running on the turning wheel without slope, performed 12 weeks, five sessions a week, with the speed of 20-25 meters per minute for 15-31 minutes. About 48 hours after the last exercise session, the rat’s kidneys were removed and frozen immediately. In order to measure the relative expression of UCP-1 gene, the method of Real Time (RT) - PCR based on SYBR-Green dye was used. The gathered data was studied using the statistical method of two-way analysis of variance. Twelve weeks of endurance exercise with medium intensity and adenosine consumption resulted a significant increase of UCP-1 gene expression (P-value: 0.001 and P-value: 0.005 accordingly). Nevertheless, the collective effect of exercise and adenosine is indicative of insignificant difference in changes of the relative expression of UCP-1 gene in visceral adipose tissue. Endurance exercise and adenosine consumption independently led to an increase in UCP-1 gene expression in the visceral adipose tissue of obese male rats Nevertheless, the collective effect of exercise and adenosine is indicative of insignificant difference in changes of the relative expression of UCP-1 gene in visceral adipose tissue. It is probable that some mechanisms be activated through the collective effect of exercise and adenosine that reduce the synergic effect of exercise and adenosine