جستجوی مقالات مرتبط با کلیدواژه « اسهال گوساله » در نشریات گروه « دامپزشکی »

The amount of antibodies produced in colostrum following injection of the trivalent Rota-corona virus and E. coli vaccine was evaluated to undrestand the priventive effects of vaccine against calf diarrhea. Forthy six and 27 pregnant cows were selected from industrial dairy herds as a treatment and control groups، respectively in Urmia Iran. A 5 ml trivalent Rota-corona virus and E. coli vaccine was injected subcutaneousely in over 7 months pregnant treatment group. After parturition a 50 ml colostrum from both groups was colleced and evaluated for antibodies using competetive ELISA method and commercial kits of Rota، corona virus and E. coli Of 46 vaccinated cows، antibodies in colostrum againt E. coli، Rota and corona virus were detected in 71. 7%، 100% and 100% and in control group were 14. 8%، 100% and 100%، respectively. The percentages of cows with +2 positive to antibodies produced in treatment group were 60. 9%، 93. 5% and 73. 9%، respectively. The highest and lowest inhibition rate in vaccinated cows for E. coli antibodies were 85% and 1%، rotavirus were 59% and 20% and corona virus were 61% and 20%، respecrively. Chi-Square tests showed significant differeces (P<0. 05) between vaccinated and control groups for E. coli antibodies (X=8. 31، P<0. 05)، cows with +2 positive antibodies produced for E. coli and control group (X=11. 7، P<0. 05)، cows with positive and negative to antibody produced for E. coli in control group (X=9. 4، P<0. 05) and cows with negative to antibodies produced and control group (X=7، P<0. 05). There were no significant differences between vaccinated group with rota and corona virus and control group. A 2. 2% of calves in treatment group and 7. 5% in control group were appeared diarrhea in which the differences was not significant. Thus، it is concluded that antibodies produced by the injection of E. coli vaccine in pregnant cows was significantly different from control group، while not for rota and corona virus vaccine. It seems that using monovalent E. coli vaccine is more reliable، economics and practicible in the prevention of calf diarrhea than polyvalent vaccine E. coli، rota and corona virus.

IDiarrhea is known as the current and economic disease among new born calves. It is characterized by dehydration، loss of macro-minerals، weakness، recumbency and death. In calves diarrhea with different etiology، loss of liquid and electrolytes are the principle signs and the cause of death too. Therefore، determination of the amounts of liquid and mainly electrolytes depletion is important in the prognosis of disease. For this reason fecal، blood and milk consumption samples from 34 diarrheic and 10 healthy calves were collected to investigate، 1) The fecal pH، specific gravity (SG)، protein، dry matter (DM) and hematocrit (PCV)، 2) The macro-mineral concentrations in samples and comparison between diarrheic and healthy calves، 3) The interrelationships among macro-minerals in fecal، blood and milk samples، and finally 4) the sequence and importance of macro-minerals leave by diarrheic feces in Urmia، Iran. Mean PCV in diarrheic calves was higher than healthy calves (P<0. 01). Mean DM (P<0. 01)، protein (P<0. 01) and pH in diarrheic calves were lower than healthy calves. Mean SG of feces in diarrheic calves was 1. 015 and DM in diarrheic calves was 2. 46 times less than healthy calves. Mean serum macro-mineral concentrations in diarrheic calves was less than healthy calves (P<0. 05). There was no different in milk serum macro-mineral concentrations between diarrheic and healthy calves. The highest and lowest percent of fecal K and Na was found in diarrheic calves، respectively. Mean comparison of the fecal and milk serum macro-mineral concentrations between diarrheic and healthy calves were also different (P<0. 05). The minimum values were found in diarrheic calves and the maximum in healthy calves. The results of correlations between serum and fecal macro-minerals in diarrheic calves were lower than in healthy calves. There was no correlations between fecal and milk serum macro-minerals in diarrheic and healthy calves. Fecal and blood Na showed correlation with the majority of macro-minerals. Thus، it is concluded that fecal excretion of macro-minerals mainly Na in diarrheic calves is remain important and must be compensate together with fluid therapy in diarrheic calves.

The major histocompatibility complex genes (mhc) encode MHC I and II molecules which present peptide fragments to T cells. Therefore these polymorphic molecules critically influence susceptibility to infectious diseases. At present study potential relationship between amino acid sequences in the antigen binding groove of different BoLA-DRB3 alleles and susceptibility or resistance to calf diarrhea was investigated. Twelve different DRB3 alleles were found among 171 calves (84 diarrheic and 87 healthy) analyesd by PCR-RFLP method. Amino acid sequences of the encoded peptide binding region were compared. 26 polymorphic positions were detected in this region. A significant association (p< 0. 05) was shown between occurrence of diarrhea and the presence of glutamic acid and tyrosine in pocket 4 and valine، glutamine and leucine in pocket 9 of peptide binding region. Thus it can be concluded that pockets 4 and 9 of the BoLA-DRB3 molecule would be involved in conferring susceptibility of calf to diarrhea.