جستجوی مقالات مرتبط با کلیدواژه "سمنان" در نشریات گروه "دامپزشکی"

In recent years, there has been a growing interest in research into the Anaplasmataceae family. In this family, there is the genus Ehrlichia, which is a compulsive intracellular and zoonotic bacterium. The main carriers of these bacteria are mites, the most common species of hard ticks that are found mainly in all parts of the world, such as Ixodes, Dermacentor, Rhipicephalus and Amblyomma. The aim of this study was to track the molecular detection of Ehrlichia ewingii among referred dogs referred to the veterinary clinic of Semnan University. In this study, 134 dogs were randomly assigned to each of them in the amount of 4-5ml of blood and they were used to extract DNA using phenol-chloroform method. Subsequently, with the help of Anaplasmataceae primers, the desired gene was traced and positive cases were recorded. Based on the results, out of the total number of reference samples, 35 samples were positive, of which 21 were male and 14 were female but none of them were Ehrlichia positive after the second round of PCR.

A cross-sectional study was conducted during four seasons from April 2016 to Mach 2017 to assess the status of cystic hydatidosis in the slaughtered camels at the Semnan municipal slaughterhouse,  in Semnan province. Hydatid cyst count and characterization were conducted based on routine meat inspection. Out of the total of 254 camels (97 females and 157 males divided in three age groups as <5, 5–10, and >10 years old) examined visually and manually by palpation and incision, 51(20/07%) were found harboring hydatid cysts in lung, liver and spleen. A significantly higher infection was detected in older camels (P<0.05) than younger ones. Of the total 51 infected, 25 (9.81 %) had hydatid cysts only in the lung, 11 (4.33 %) in the liver, 16 (6.29 %) in both the liver and lungs, and 2 (0.78 %) in lung, liver and spleen. Out of the 70 viscera harboring hydatid cysts, the highest (58.57%) was the lungs followed by the liver (38.57%), and the spleen (2.85%). In addition, out of the total 405 cysts collected, 16.79% were calcified cysts. The rate of cyst calcification was higher in the liver than in the lung. The results showed that hydatidosis is one of the major parasitic diseases in the study area. In light of the result obtained and the current situation in Municipal abattoirs in Semnan province, serious attention for its prevention and control is warranted.

The Mediterranean form of visceral leishmaniasis (VL), or kala-azar, is a potentially fatal vector-borne zoonotic disease caused by Leishmania infantum, which is sporadic in most parts of Iran, while in other parts it is considered endemic. Domestic dogs (Canis familiaris) are the primary reservoir hosts for human VL. The objective of the present study is to determine the seroprevalence of VL in the dogs with owners in rural areas of Semnan. For this purpose, 140 blood samples from dogs, which had no clinical signs, were taken randomly. Blood samples were tested by direct agglutination test (DAT) to detect the anti-Leishmania antibodies in dogs, using a cut-off value of ≥1:80. We considered anti-leishmanial antibodies titers at ≥ 1:320 with a clinical sign as Leishmania infection and at ≥ 1:80 with no clinical symptoms as parasitologically infected. Pathological specimens including spleen, liver, and lymph nodes from an infected dog (1:20480) were prepared for Dub smear in Laboratory and staining with Giemsa. Also, the stages of amastigote leishmaniawere had been observed in isolated tissues. The anti-Leishmania antibody (≥ 1:320) was detected in 4 dogs (2.9 %) of the total 140 studied dogs. No significant difference between VL infection and gender & age was found. The results of this study showed that VL with low endemicity is circulating in dogs of Semnan.

Abstract Brucellosis is a disease of animals caused by Brucella species and is transmissible to humans. This study was undertaken to determine the sero-prevalence of the disease in camels slaughtered in Semnan abattoir during a 4-month period in 2014. To investigate brucellosis in these animals, serological examinations including Rose Bengal Plat Test (RBPT), Wright and 2-ME were performed on 150 camel’s serum samples. Positive results were obtained in 14 (9.3%) camels by RBPT, then, Wright and 2-ME assays were performed to assess the titers of Anti-Brucella Antibodies, respectively. There was no relation observed between the seropositivity and age and sex of the tested animals. The study revealed that the prevalence of Brucellosis among camels slaughtered in Semnan and the results reflect the necessity of control programs and surveillance of the disease needed to be adopted.

To assess hard ticks population on Sangsari sheep breed under the effect of weather’s parameters such as duration of sun shine، humidity، ambient temperature، altitude، 5% of a flock (almost 1000 sheep in mountains and fields of Semnan and Tehran provinces) were randomly separated and were observed monthly. During a year، a total of 1505 hard ticks (635 females and 870 males) containing Dermacentor marginatus، Dermacentor raskemensis، Hyalomma marginatum marginatum were separated. Infestation rate were vary from 0% in two last months of autumn and winter to 82% in September. Intensity of infestation in different times، was vary from 0 to 12. 45 ticks on each sheep. Results showed that there is a significant relation between duration of sun shine and humidity with prevalence of infestations (p<0. 01). Average prevalence of infestation in different months was 56. 85% and average of intensity of infestations was 7. 56 ticks on each sheep. Also it was found out that altitude is one of the parameters which can affect the site of ticks on sheep’s body.

Poultry products have been recognized as major sources of human gastrointestinal disease caused by Salmonella spp. and several outbreaks have been reported where eggs were the source of human infection. This study was carried out to determine the prevalence of Salmonella spp. in the quail egg interior contents from retail stores of Semnan, Iran and to characterize the isolated Salmonella serovars via serotyping and Multiplex PCR techniques. 140 packages of quail eggs (each package containing 12 eggs) were collected from different batches during summer 2010 and tested for the presence of Salmonella through conventional culture and serotyping methods. From these samples, S. enteritidis was detected in the egg contents of one package (0.71 %) out of 140 packages. This isolate was confirmed by Multiplex PCR generated amplification products for a random sequence that is specific for the genus salmonella and spv and sefA genes. According to our results, S. enteritidis is the most prevalent serotype of quail egg content contaminant in the Semnan area of Iran and the multiplex PCR method could be used as a reliable method of identifying Salmonella serovars.