جستجوی مقالات مرتبط با کلیدواژه "لینگواتولا سراتا" در نشریات گروه "دامپزشکی"

Linguatula serrata is the cause of linguatolosis, a parasitic disease common to humans and animals. Canines and ruminants are important final and intermediate hosts for the parasite respectively. The objective of this study was to determine the seroprevalence of Linguatula serrata infection in goats of different regions in Ilam province by using the enzyme-linked immunosorbent assay (ELISA). The ELISA test was designed to detect Linguatula serrata infection in goats using excretory-secretory antigens, serum samples (62 negative and 40 positive) and anti-goat IgG conjugate.  A total of 805 goat serum samples collected from five regions of Ilam province were tested by ELISA.Seroprevalence of parasite infection among goats in the province of Ilam was 28.57 percent, along with 65.8% in Ilam (center), 63.4% in Ivan (north), 19.2% in Dehloran (south), 16.9% in Chardavol (east), and 9.9% in Mehran (west).The seroprevalence varied significantly between different areas of the province (p<0.01). There was no significant difference between the rate of infection in age groups 1- <2, 2- 3>, 3- ≥4 with 27.7%, 29.5%, 28.3%, respectively (P = 0.849), and also in resident (29.1%) and nomadic (26.9%) herds were not observed (P=0.545).The results of this study indicate that the ELISA test can be used as one of the convenient serological methods to detect Linguatula serrata infection in goats. To control and manage the disease, educational and preventive measures are recommended due to the presence of infection in livestock of the region.

Linguatula serrata is a toungue-shaped parasite and one of the ethiologic causes of zoonotic diseases between human and animals. Human can be infected by both the nymph stage and the egg, causing a disease condition called nasopharyngeal linguatulosis or halzoun syndrome, and a condition called visceral linguatulosis respectively.In this study an eight year old female camel with history of infertility was scarified in autumn 2017 in one of the vilages of Garmsar county in Semnan province northeast of Iran. Mesenteric and mediastinal lymph nodes were inspected postmortem for the presence of L. serrate nymphs and proper samples obtained and transferred to parasitology department for further investigation.Nymphs were examined and detected through sectioning and maceration of lymphatic glands and digestion in digestive solution. After completing the required tests, lymph node samples were recognized as positive and the case was reported as the first case of Linguatulosis in camel in Semnan province.

This study was performed to determine the prevalence of Linguatula serrata in sheep and goats by observing the parasite in the mesenteric lymph nodes and measuring the antibody titer by indirect haemagglutination test and also evaluating the activity of serum complement activity by classical method and lysozyme. 791 samples of blood and mesenteric lymph nodes from sheep (456) and goats (335) were collected in the slaughterhouse of Ahvaz (Khuzestan province). The results showed that 7.8% of all ruminants in the present study were infected with L. serrata. There was no significant difference in the level of complement activity by classical method and lysozyme activity between infected and non-infected animals. Also, using an indirect haemagglutination test, in addition to accurately identifying infected samples from non-infected animals, the levels of IgG and IgM antibodies against L. serrata were estimated. IgG levels in infected samples were higher than IgM levels and the levels of these antibodies were significantly lower in non-infected samples. Overall, the present study suggests that this parasite in the intermediate host may escape from some important components of the innate immune system (such as the complement and lysozyme). Although the results of the present study showed that infection with the parasite increased the level of some antibodies, but these changes do not seem to provide the conditions for the eradication of the parasite.

Linguatula serrata is a zoonotic parasite causing Halazoun syndrome in humans. Consumption of raw or semi-cooked infected edible offal induces the infection in human.
The main objective of this study was to investigate the outbreak and molecular characterization of Linguatula serrata in sheep and goat of Yazd slaughterhouse.
To determine the prevalence and severity of Linguatula serrata, mesenteric lymph nodes of 200 slaughtered sheep and 200 slaughtered goats in the Yazd industrial slaughterhouse were examined. DNA extraction was performed using commercially DNA extraction kit as manufacturers’ protocol. In order to genetic evaluation, the partially 18srRNA gene as a target was amplified using the specific primer pair which was designed by Primer3 software.The PCR product sent for sequencing and the sequence was BLAST. Data were then analyzed using SPSS version 16.0 and by the Pearson correlation test and χ2 at a significance level of 0.01.
In the present study, prevalence of the infection of slaughtered goats and sheep was 25.5% and 22.5%, respectively. No statistically significant difference was observed between the prevalence of this parasite in different ages and sexes groups (goats and sheep). The results of genetic evaluation showed no variation between this isolate in comparison with the ones in GenBank.
This study was the first report of molecular identification of Linguatula serrate in Iran. Considering high prevalence of infection in domestic animal and lack of knowledge and hygienic practice of the people about consumption of animal offal infection of the people to Linguatula serrata is probable. Therefore, in this context, using appropriate and reliable diagnostic methods for detection of infection in abattoirs as well as educating people on the proper use of animal offal is effective steps to prevent this disease.

Linguatula serrata is one of the parasitic zoonoses. The adults, larval stages of the parasite live in nasal airways of canids and visceral organs of herbivors respectively. The aim of this study was to determine the seroprevalence of L. serrata nymph infection in sheep from west of Iran by indirect enzyme-linked immunosrobent assay (ELISA). Blood samples (n=1048) were taken from sheep of two province (Ilam (n= 598) and Kermanshah (n=450)) in west of Iran ( at least five different cities of each province). The collected sera were tested by ELISA using excretory-secretory antigens of L. serrata nymph and anti IgG sheep conjugate. Data were classified according to sex, ages of the animals (1-0.05). Seroprevalence was significantly increased with the increase of age, lower (12.1%) in 1-

Linguatula serrata as a cosmopolitan parasite is one of the parasitic zoonoses. The adult parasites live in the nasal airways or frontal sinuses of canids as the definitive hosts and larval stages in the liver¸ lung and mesenteric lymph nodes of ruminants as intermediate hosts. Humans may be infected by eating eggs or nymphs of the parasite. All intermediate hosts may be infected without showing any clinical signs. No serological methods presented for detection of the infection in herbivorous animals. In this study، for the first time، an indirect ELISA test was developed for the detection of Linguatula serrata infection in sheep by using somatic and excretory-secretory antigens، isolated from nymphs of the parasite. Nymphs of the parasite were collected from mesenteric lymph nodes of infected sheep and used for preparation of somatic antigens by sonication (250 nymphs/10ml، PBS- RPMI1640). The excretory-secretory antigens were prepared by incubation of the larvae (200 nymphs/10ml) in RPMI-1640 media for 24 h. Appropriate dilutions of sera، anti-Sheep IgG conjugate، somatic and excretory-secretory antigens were determined as 1:100، 1:5000، 1:40 and 1:4 respectively. Evaluation of the ELISA was performed by 121 sera (73 positive and 48 negative). The sensitivity، specificity، positive predictive value (PPV)، negative predictive value (NPV) and accuracy of the ELISA for somatic antigen were 90. 4%، 75. 0%، 84. 6%، 83. 7%، 84. 2% and those for excretory-secretory antigen were 89. 0%، 93. 8%، 95. 6%، 84. 9% and 90. 9%، respectively. Data analysis of the present study by SPSS software and Mac Nemar Test showed that excretory-secretory antigens are better than somatic antigens for detecting of the infection in sheep. Therefore the ELISA test using excretory-secretory antigen of nymphs of Linguatula serrata can be used for detection of infection in sheep، especially in epidemiologic studies and developed for other hosts.

Linguatla serrata is a zoonotic parasite which causes different forms of liguatulosis in humans, carnivores and ruminants. The most important way of human infection is injection of vegetables, fruits and water contaminated by parasite eggs and through nasal and oral secretions and feces of carnivorous especially stray dogs. Also, consumption of raw and under cook meat of sheep, goats, cattle and other herbivores is another risk factor in human infection by Linguatla serrata. This study was conducted in order to determine the infection rate of dogs by Linguatla serrata in the city of Urmia. In the present study, 37 dogs including 22 male and 15 female animals from different parts of the city were studied. The frontal sinuses, nasal turbinates, brain cavity, nasopharynx and eustachian tubes were examined for adult Linguatla serrata. The recovered parasites were fixed in 10% formalin solution, cleared by lactophenol and stained with azocarmin. Thirty of the studied dogs (81.01%) were infected by Linguatla serrata. The results indicated that body weight, age, sex and geographical locations had no significant effect in the prevalence rate of the parasite. The number of parasites recovered from each dog ranged from 1 to 7 with an average of 2.93 in each dog. The length of the mature linguatula varied from 35-50 mm in females and 2-18 mm in males. The greatest number of parasites was found in the cranial part of the frontal sinus with 7 parasites.