جستجوی مقالات مرتبط با کلیدواژه "کلرامفنیکل" در نشریات گروه "دامپزشکی"

Residues of drugs used in poultry farming are recognized as significant pollutants, posing potential risks to consumer health. Among these, antibiotics are widely administered to combat infectious diseases, with residues frequently detected in various products, including chicken meat. Given the high consumption of chicken in the country, it is crucial to monitor commonly used antibiotics such as chloramphenicol and florfenicol in these products. In this study, a combined method of microwave-assisted extraction and homogenous liquid-liquid microextraction was employed to extract chloramphenicol and florfenicol from chicken meat samples. The extracted antibiotics were subsequently analyzed using high-performance liquid chromatography with a diode array detector. Under optimal conditions, extraction recoveries were 60% for chloramphenicol and 66% for florfenicol, demonstrating the method’s efficiency. The detection limits were 0.20 ng/g for chloramphenicol and 0.17 ng/g for florfenicol, indicating the method’s sensitivity to low concentrations. Notably, florfenicol residues were detected in two of the ten chicken samples tested.

Nowadays، uncontrolled and frequent use of antibiotics may cause emergence of microbial resistance among pathogenic agents. Therefore، the use of new synthetic and natural antimicrobial compounds is inevitable. One source of natural compounds in this respect comes from plants. The purpose of this study as to examine the antibacterial effects of peel extracts from sour and sweet pomegranate. Methanolic extracts of sour and sweet pomegranate peels and aqueous solutions of tetracycline and chloramphenicol were prepared. Antibiogram tests using disk diffusion technique and serial dilution method were performed against ten pathogenic bacteria isolated from animals، and relative minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were also determined for the above compounds. The greatest zone of inhibition induced by the action of pomegranate peel extracts was obtained for Staphylococcus aureus (about 25 mm) and the smallest zone of inhibition was obtained for Pasteurella multocida (about 9 mm). In addition، the lowest MIC and MBC values of pomegranate peel extract were obtained for Staphylococcus aureus (7. 8 and 62. 5 mg/ml، respectively). Results of serial dilution tests indicate that bactericidal effect of sour pomegranate peel extract was more than that for sweet pomegranate peel extract; and sweet pomegranate peel extract exerts a bacteriostatic action against bacteria. The antibacterial effect was greater against Gram-positive bacteria compared to that for the Gram-negative bacteria. Effects of these extracts were considerably lower than those for tetracycline and chloramphenicol. In conclusion، methanolic extracts of pomegranate peels exhibit relatively good bacteriostatic and bactericidal effects.