جستجوی مقالات مرتبط با کلیدواژه « کلی باسیلوز » در نشریات گروه « دامپزشکی »

The purpose of this research is to identify and isolate antibiotic-resistant Escherichia coli strains and determine the antibiotic resistance pattern in 30 Escherichia coli isolates isolated from broiler chickens with colibacillosis in Behbahan city. The antibiotic resistance pattern of all isolates was determined against 15 important and common antibiotics in Iran's poultry industry using the disk diffusion method. The percentage of resistance to difloxacin, enrofloxacin, trimethoprim, chlortetracycline, erythromycin, tylosin, tiamulin, doxycycline, florfenicol, lincomycin, danofloxacin, neomycin, colistin, amikacin and gentamicin is 100, 100, 100, 96/6, 96/6, respectively. 96/6, 90, 76/66, 66/6, 63/33, 60, 53/33, 26/66, 10 and 0 were observed. 17 drug resistance patterns were identified among 30 Escherichia coli isolates to 15 commonly used antibiotics in the poultry industry, of which 26 isolates (86.67%) belonged to more than one pattern, while the other 4 isolates (13.33%) each They belonged to only one pattern. The results of this study showed that the resistance of the isolates to the majority of commonly used drugs in Iran's poultry industry is high, which makes it necessary to implement a strong national monitoring plan for antimicrobial resistance and the principled use of antibiotics.

Escherichia coli is the causative agent of colibacillosis in poultry leading to heavy economic losses and increased antibacterial use in poultry flocks with a consequence of increased drug resistance. In order to reduce drug resistance, as a global problem, and to establish better health among humans, animals and environment, a periodical monitoring of drug resistance rate is required. The aims of this study were to investigate the resistance of Escherichia coli isolates to different antimicrobial agents and also to detect florfenicol (cfr, fexA, floR) and colistin (mcr-1) resistance genes among isolates. One hundred Escherichia coli isolates originated from typical lesions of colibacillosis were collected from poultry referred to a private laboratory in Tehran and University bacterial collection and the drug resistance of isolates to 16 antimicrobial agents including ampicillin, neomycin, gentamicin, enrofloxacin, flumequine, difloxacin, chloramphenicol, florfenicol, Fosbac, erythromycin, colistin, tetracycline, oxytetracycline, trimethoprime+sulfa, linco-spectin and doxycycline was determined by using disk diffusion method. Then, the chromosomal and plasmid DNA were extracted and florfenicol (cfr, fexA, floR) and colistin (mcr-1) resistance genes were detected among all isolates by using polymerase chain reaction test (PCR). Based on antimicrobial susceptibility test, the highest resistance was observed to erythromycin, doxycycline and tetracycline and the lowest resistance rate was found to linco-spectin, gentamicin and Fosbac. All isolates were resistant to at least one and 10% of isolates were resistant to at least 12 antimicrobial agents. The 16% of the isolates belonged to one identical pattern and 35% of isolates each belonged to a separate pattern. Among 85 tested isolates, 40 and 52.94% of the isolates showed floR gene on their plasmid and chromosomal locations, respectively. However, no isolate was positive for fexA, cfr and mcr-1 resistance genes. The findings of this study demonstrated the high frequency of resistance to commonly used antimicrobial agents among E. coli isolated from colibacillosis. Detection of resistance genes increases the researchers’ knowledge on the epidemiology of drug resistance. This information indicates the necessity for implementation of the right management programs for poultry farms and rational antimicrobial therapy besides periodic antimicrobial susceptibility monitoring.

Among diseases caused by Escherichia coli, there is a severe systemic form termed colisepticaemia, which is characterized by the presence of E. coli in the blood, and colonization of organs including the heart, liver and spleen. The aim of the present study was to investigate different phylogenetic groups of E. coli isolated from broiler breeder with colibacillosis in Urmia. In this study, eighty swabs collected from liver and lung were cultured on MacConkey agar plates. Pink color colonies were isolated and confirmed as E.coli by biochemical tests and followed by multiplex-PCR to identify different phylogenetic groups. Out of 80 samples 21 isolates were identified as E.coli. Eight of isolates (38%) were belong to group A, 2 of them (9.5%) were belong to group B1, 6 of them (28.6%) were belong to group B2 and 5 of them (23.8%) were belong to group D2. According to the results of present study different phylogenetic group were observed in breeder herds. Most of them were classified as group A which is commensal. Studies showed that pathogenic E.coli has a considerable antibiotic resistance rate which might be transmitted to broilers in different ways and poses economic constraint to poultry industry. Thus, important strides must be made on eradication of different pathogenic E.coli.

Escherichia coli is an opportunistic organism which can cause Colibacillosis syndromes (colisepticemia, pericarditis, perihepathitis, salpingitis and …). E.coli has four phylogenetic groups including A, B1, B2 and D. Phylotyping is an adequate method for surveying the dissipation type of the phylogenetic groups of E.coli isolates in different regions. The present study was conducted to determine the phylogenetic groups of E.coli in Zabol city. In order to determine the frequency of the phylogenetic groups of E.coli in Zabol, 144 broilers suspected of Colibacillosis were sampled and samples were transferred to laboratory in TSB. After culturing and applying some common biochemistry tests, a total number of 100 E.coli were isolated. DNA of all isolates was extracted by boiling method and phylogenetic groups were determined by triplex PCR procedure. In this study; 36 %, 27 %, 23 % and 14 % of 100 E.coli isolates belonged to B1, D, A and B2 phylogenetic groups; respectively. The present study concluded that almost all of E.coli samples which were collected from broilers suspected of Colibacillosis in Zabol belonged to B1 phylogenetic group.