جستجوی مقالات مرتبط با کلیدواژه « ethanol extract » در نشریات گروه « دامپزشکی »

Infectious diseases widely occur through bacteria such as Salmonella spp. in different hosts such as domestic animals and human.

The aim of this study was to isolate Salmonella Typhimurium bacteria from poultry feces and in vitro assessment of antimicrobial effects of 9 species of Zagros native medicinal plants.

Ethanol extracts of jooshir, shallots, Iranian eremurus, artichokes, sheng, amaranth, sorrel, oregano and harmel were extracted through rotary vacuum system. Furthermore, 12 strains of Salmonella Typhimurium were isolated from poultry feces in Sistan region. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts were determined through micro dilution method. Sensitivity of different strains to antibiotics was tested through standard Kirby-Bauer diffusion disk methods.

Salmonella Typhimurium strains are resistant to Penicillin (100%), Ampicillin (100%), Tetracycline (16.6%), Amikacin (8.3%) and Oxacillin (75.0%). Results of the antimicrobial assay have shown that extracts of amaranth, jooshir and artichokes had the highest MIC against Salmonella Typhimurium.

Results of the current study showed that according to the antibiotic resistance of Salmonella Typhimurium isolated from poultry, antibiotic treatment, in particular with penicillin, ampicillin and oxacillin with the bacterium resistance of 75 to 100% would not be effective. In contrast, using plant ethanol extracts especially jooshir, amaranth and artichokes could be recommended as an alternative to conventional antibiotic treatments.

In spite of novel progresses in the methods for foodstuff’s preparation and production, consumer’s health and safety in the public hygiene import increasingly. One of the ways to control sickening microorganisms is the use of chemical retentive in the food. Today consumers are complaining to use natural retentive that are derivatives of herbaceous resources. As for harmful effects of food’s synthetic and chemical retentive. This study performs as for determining antibacterial Effects of Digitalis plant’s extracts on Staphylococcus aureus pathogenic bacteria in the foodstuff. In this study the ethanol extract alone and in combination with ciprofloxacin antibiotic on gram-positive bacteria, Staphylococcus aureus (ATCC25923), were evaluated by micro broth dilution method. MIC test results for Digitalis plant extract and Ciprofloxacin antibiotic evaluated 4.9 mg/ml and 0.5 μg/ml. effect of both extract and antibiotic results showed that growth of Staphylococcus aureus in the simultaneous use of 3.5 mg/ml extract and 0.28 μg/ml was inhibited by antibiotics. Evaluation of MBC tests for Digitalis plant extract and ciprofloxacin antibiotic evaluated 7.1 mg/ml and 1 μg/ml, results show that the combination of 4.2 mg/ml of extract and 0.14 μg/ml antibiotics cause the bacteria is eliminated. Concomitant use of alcohol extract and antibiotics, decreasing 48 percent of antibiotic to inhibit the growth of Staphylococcus aureus, and simultaneous use of alcohol extract and antibiotic, antibiotic use decreased 86 percent in the MBC for Staphylococcus aureus.

Nowadays, multiple drug resistance and side effects such as residual drug, hypersensitivity, immune-suppression and allergic reaction has developed among humane society and animal due to the irregular use of commercial antimicrobial drugs. Therefore, use of natural compounds such as medicinal herbs for therapeutic goals is recommended. In the present study, an attempt has been made to investigate the antibacterial characteristics of Nigella sativa against ten different wild veterinary bacterial pathogens. Identification of clinical isolates from different bacteria was done on the basis of morphology, cultural characteristics and biochemical reactions. MIC and MBC were determined by macro broth dilution method using serially two folds diluted of ethanol extracts from Nigella sativa. The main bactericidal and bacteriostatic activity of ethanol extract of Nigella sativa against pathogenic bacteria were respectively as MBC (mg/ml) and MIC (mg/ml) Yersinia enterocolitica (200-100), Listeria monocytogenes (25-12.5), Corynebacterium pseudotuberculosis (12.5-12.5), C. renale (6.25-3.125), Brucella abortus (50-25), Pasteurella multocida (6.25-6.25), Mannheimia haemolytica (3.125-3.125), Escherichia coli (200-100), Trueperella (Arcanobacterium) pyogenes (6.25-3.125), Staphylococcus aureus (50-50). The highest antibacterial activity was shown against M. haemolytica with the MIC of 3.125 mg/ml and the least for E. coli and Yersinia enterocolitica with the MIC of 100 mg/ml. The result of this study showed that ethanol extract of Nigella sativa exhibited an inhibition in the growth of all examined pathogenic bacteria and this extract or the compounds derived from it can be used in control of infectious agent.