جستجوی مقالات مرتبط با کلیدواژه « seminal plasma » در نشریات گروه « دامپزشکی »

This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the first and second ejaculations and their relationships with semen quality parameters in Fleckvieh bulls. Repetitive ejaculates were separately collected, analyzed, and frozen from the sires. Progressive motility of frozen-thawed semen (PMFT) was considered the main factor for more data classification into three following groups: <40.00%, 40.00 - 50.00%, and >50.00%. Seminal plasma trace elements and enzymes were determined using atomic absorption spectrometry and ELISA, respectively. The results revealed significant differences between the first and second ejaculations. Semen concentration, SOD, GPx, and Fe were different in ejaculations. Although PMFT groups in different ejaculations did not show significant differences, there was significant alteration between different PMFT groups and first and second ejaculations. All frozen-thawed semen CASA parameters (except lateral head displacement) were associated with fresh motility parameters and before and after thawing sperm viability. Also, a correlation between seminal Zn concentration with fresh semen gross and progressive motility, average path velocity, and beat cross frequency, Cu with SOD and Fe and semen concentration was observed. CAT was associated with fresh and frozen-thawed sperm motility parameters except for lateral head displacement and angular displacement. Although our findings showed differences between the first and second ejaculations in some parameters, PMFT, which is the most important indicator for estimating bull fertility, was not different between them.

Artificial insemination is a well-established and widely used method for genetic improvement in cattle breeding industry. Recently, researchers have shown an increased interest in the cryoprotective effects of minerals and antioxidants on semen. Previous studies on calcium (Ca) and magnesium (Mg), two main macro-minerals, have mainly investigated their roles in mammalian spermatogenesis and fertility. In addition, the experimental data examining the semen content regarding these minerals and antioxidants from different animal species are rather controversial and there is no general agreement about their associations with semen quality. Therefore, this study was conducted to assess the seminal plasma concentrations of Ca, Mg and total antioxidant capacity (TAC) in first and second ejaculations of dual-purpose Fleckvieh bulls and to link them to the sperm characteristics of fresh and frozen-thawed semen. Sperm progressive motility after thawing was used to classify the data into three groups: < 40.00%, 40.00 to 50.00% and > 50.00%. The measurements of two minerals and TAC were carried out using spectrophotometry and enzyme-linked immunosorbent assays, respectively. The results showed that there were significant differences in several parameters of semen quality between first and second ejaculations. No significant differences were also found on Ca and Mg concentrations and Ca/Mg ratio. The TAC level was significantly higher in the first ejaculation than the second one. The findings of this study suggest that TAC is a potential marker for bull semen quality assessment in the frozen semen production industry.

One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. The objective of this investigation was to evaluate recovery, motility, and plasma membrane integrity of alpaca spermatozoa after centrifugation in one of two different solutions at one of three different combinations of speed and time. A total of 24 ejaculates was recovered from seven reproductively sound Huacaya males using a modified artificial vagina (AV) after training the animals for semen collection. A 2 × 3 factorial treatment arrangement was utilized for this study. Ejaculates were divided into fractions for centrifugation in one of two solutions (Tris extender or PureSperm®80 density gradient solution) at one of three combinations of speed and time (492 × g for 15 min, 1968 × g for 10 min, or 4448 × g for 7 min). The experiment was replicated eight times. Analysis revealed that centrifugation at 4448 × g for 7 min in PureSperm80 provided a high recovery rate of spermatozoa with the highest sperm motility and functional integrity of plasma membrane post-centrifugation. Results suggest that adoption of this procedure (centrifugation at 4448 × g for 7 min in PureSperm80) in the initial processing of alpaca ejaculates may enhance subsequent ability to use semen for AI and other assisted reproductive biotechnologies in this species.

Two experiments were designed to examine the effects of crude seminal plasma (CSP) exposure of ejaculated and epididymal spermatozoa before freezing. Experiment 1، two consecutive ejaculates were collected (n=4) within 5 min، the second one was carried out in a tube containing Fiser extender (coated spermatozoa); by centrifugation، seminal plasma was removed from coated ejaculates. The pellets were split into three parts and 0، 50 and 100% CSP (v/v) was added and they were frozen. Samples were thawed and incubated at 37°C for 6 h. The highest progressive motility (10. 41%) and the highest hypo-osmotic responses (12. 62%) were found for the coated spermatozoa without CSP at 6 (P<0. 05). The maximum viability was found for coated spermatozoa without CSP at 0 (29. 4%) and 6 (17%، P<0. 05). Experiment 2، epididymal spermatozoa were recovered (n=6)، pooled and split into three fractions and 0، 50 and 100% CSP and the diluents were added، after that they were frozen. Thawed epididymal spermatozoa were incubated at 37°C for 6 h. The highest (33. 3%) and lowest (25%) progressive motility were found for the epididymal spermatozoa without CSP and the epididymal spermatozoa with 100% CSP at 0، respectively (P<0. 05). The highest (19. 37%) and the lowest (9. 38%) viability were related to the epididymal spermatozoa with 0 and 100% CSP at 6، respectively (P<0. 05). Under the conditions of the current study، the addition of CSP to the ram epididymal and coated spermatozoa strengthened the detrimental effect of the freezing procedure.

The Indian riverine buffaloes are more susceptible to variations in the environment. These variations affect seminal plasma proteins، which in turn affect fertility. This study was conducted to evaluate variations in the Indian Bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) during winter and summer seasons، and its relation with sperm motility and viability. The nine ejaculates from three animals were from winter season while the other nine ejaculates from the same animals were from summer season. The semen samples collected from all bulls in both seasons have similar characteristics of mass activity and total concentration. However، sperm viability was significantly higher in winter season. The 2D-PAGE pattern displayed 42، 29 and 28 protein spots during winter season while 44، 29 and 29 spots during summer season for the first، second and third bull، respectively. Ultra high performance liquid chromatography-mass spectrometry (UPLC-MS) was performed to identify expressed protein spots in winter and summer seasons. During both seasons، four commonly expressed protein spots B6 (pI 8. 5، Mr 122. 54 kDa)، B7 (pI 9. 7، Mr 89. 98 kDa)، B9 (pI 9. 3، Mr 19. 72 kDa) and B10 (pI 9. 7، Mr 16. 80 kDa) were identified as glucose phosphate isomerase، epididymal secretory protein E1، peroxiredoxin 5 precursor and tubulin polymerization-promoting protein، respectively. These proteins are involved in either the mechanism of sperm maturation or structural formation. In addition، B37W (pI 6. 0، Mr 32. 88 kDa)، B48W (pI 8. 2، Mr 80. 14 kDa)، B59W (pI 5. 6، Mr 90. 37 kDa) and B61W (pI 5. 8، Mr 15. 34 kDa) were differentially expressed protein spots in winter season only. Spots B37W، B48W، B59W، B61W، B6، B7، B9 and B10 were identified as phosphoglycerate kinase LOC538592، androgen regulated protein، hypothetical protein LOC514663، sorbitol dehydrogenase، glucose phosphate isomerase، epididymal secretory protein E1، peroxiredoxin 5 precursor and tubulin polymerization-promoting protein family member. These proteins are known to have modulating properties on sperm motility and viability and also to provide high energy source to sperm. The better fertilizing ability of bull during winter season can be due to the differentially expressed proteins.

In order to determine calcium (Ca), magnesium (Mg) content and total antioxidant capacity (TAC) of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested by centrifugation and its Ca and Mg content were estimated and its TAC determined. The Ca and Mg content of the seminal plasma (Mean ± SEM) were recorded as 22.36 ± 0.52 mg dl-1 and 11.94 ± 0.36 mg dl-1 respectively, while, its mean TAC value was 1.50 ± 0.02 mmol L-1. The mean Ca value was highly associated with sperm progressive motility, gross motility, viability (P = 0.000 for all), negatively with semen volume (P = 0.01), and with Mg and TAC values (P = 0.000 for both). The mean Mg values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and TAC (P = 0.000 for all) and negatively associated with semen volume (P = 0.014). The mean TAC values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and Mg (P = 0.000 for all). For further clarification of these associations, the data was categorized in three groups of excellent (Ex, >90% motile, n = 33), good (Go, 80-89% motile, n = 15) and moderate (Mo, <79% motile, n = 6) according to their percentage of sperm motility. The mean progressive motility in Ex group was 92.24 ± 0.51%, in Go group it was 81.66 ± 0.62 %, and in Mo group it was 71.66 ± 1.05 %. The mean Ca, Mg and TAC values were respectively recorded as 25.12 ± 0.29 mg dl-1, 13.78 ± 0.20 mg dl-1, and 1.57 ± 0.009 mmol L-1 in Ex, 18.74 ± 0.63 mg dl-1, 9.14 ± 0.33mg dl-1, and 1.42 ± 0.044 mmol L-1 in Go, and 17.34 ± 0.18 mg dl-1, 8.06 ± 0.25 mg dl-1, and 1.23± 0.05 mmol L-1 in Mo groups. The associations in groups are discussed. These results show that seminal plasma Ca and Mg content and TAC are associated with semen characteristics, and synergistically have an effect on motility and viability of the spermatozoa after ejaculation, which are important factors in semen fertility.

It is of critical importance to understand the modalities of BLV presence in semen, especially with regard to artificial insemination (AI). Presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. In this study paired blood and semen samples from 45 bulls were assessed for the presence of part of gag gene and antibodies to BLV in blood, semen and cell-free fraction of the semen (seminal plasma). Proviral DNA was detected in 5 out of 45 seminal plasma samples. PCR products were sequenced and submitted to gene bank. This data strongly suggested that seminal plasma of seropositive bulls can be positive in PCR.

The objective of this study was to investigate aspartate-amino-transferase (AST), g-glutamyl-transferase (GGT), alkaline phosphatase (ALP), lactate-dehydrogenase (LDH) and acid phosphatase (AcP) activities and semen parameters (volume, pH, concentration, total sperm number (TSN), progressive motility, dead sperm, total morphological defect (TMD) and hypo-osmotic swelling test (HOST)) in seminal plasma of Arabian horses. Furthermore, correlations between enzyme activities and semen parameters were examined. The study was performed using seven healthy Arabian stallions of proven fertility, between 11 and 17 years of age, from the Karacabey Stud Farm in Bursa, Turkey. Overall, 21 semen samples were collected from stallions during the breeding season from March to May. A significant negative correlation was observed between semen volume and concentration of TMD, AST, ALP (P<0.05) and LDH (P<0.01). pH showed a significant correlation with live:dead ratio, GGT activity (P<0.05) and progressive motility (P<0.01). All semen concentrations correlated significantly with TSN, TMD, ALP, AcP (P<0.01). Furthermore, significant correlations were found between live:dead ratio and TSN, HOST (P<0.05); TSN and ALP, AcP (P<0.01); progressive motility and HOST (P<0.01), GGT (P<0.001); AST and ALP, LDH, AcP (P<0.001); GGT and LDH (P<0.05); ALP and LDH, AcP (P<0.01) and LDH and AcP (P<0.001). No significant correlation was found between enzyme activities in stallion seminal plasma and semen parameters in different months, except for pH and HOST.