جستجوی مقالات مرتبط با کلیدواژه "sequence" در نشریات گروه "دامپزشکی"

Oestrosis is nasal myiasis, which results from infestation with the larvae of flies that belong to the genus Oestrus. Nasal discharge and sneezing are the most common clinical signs in infected animals. Myiasis larvae were collected from sheep in different climatic regions of Iran. Morphological identification of the larvae was made based on the diagnostic keys. The species was confirmed by PCR amplifying the partial fragment (610 bp) of the mtCOI gene. Genetic distance was assessed in COI sequences, and a phylogenetic tree was drawn. Sequencing showed no difference in the partial COI gene among the Iranian isolates, and this gene had a high similarity with the sequences of O. ovis isolates from Iraq, Bosnia and Herzegovina, and Croatia. The present study provided the first molecular dataset for O. ovis species, which is crucial for the phylogenetic relationships assessment and the molecular identification of these parasites.

Fungal diseases are the common cause of death in wild animals and birds of prey. This study was designed to investigate the development of fungal infections among wild birds in Denmark. In this study, fungal samples were isolated from such sources as Barn swallows' feathers, White stork, and birds of prey. The fungal species were isolated by direct culture of feathers on SD Agar with chloramphenicol and incubated at 28±2ºC. The fungal genomic DNA was isolated from each species, PCR reaction was performed, and the resulting fragments of the 18S rRNA DNA were sequenced and used for identification. A comparison between the resulting fragments was made to find out the percentage of similarity among the different fungal species. The multiple sequence alignment showed percentages of similarities ranging from 39% to 99%. To sum up, the 18S rRNA DNA sequence has been evolved dramatically even within the same species, while still conserved in others. It is a useful tool to be used for the identification of fungal species as it reduces time. Moreover, according to the results, there were no comprehensive high homology percentages among the species infecting the same bird.

Fasciola species are parasitic trematode with world wide distribution that infects wild and domesticated herbivores, particularly ruminants. The aim of the present study was to investigate the intra species variations of F. gigantica, from goats and buffalos isolates in two common geographic climates of Iran.
Fasciola species were collected from goat, buffalo, sheep, and cattle in different regions. Cytochrome c oxidase I (COX1) of mitochondrial DNA (mt-DNA) was amplified from individual trematodes by polymerase chain reaction (PCR), using universal primers, and the amplicons were consequently sequenced and sequencing data were analyzed, using Clutal W software against the GenBank database.
A monomorphic DNA segment of approximately 499bp was seen in Fasciola isolates. The results of the amino acid sequence alignment defined strictly conserved amino acid residues in buffalo isolates of F. gigantica and partially conserved residues for goat isolates of F. gigantica. There are four tandem amino-acid replacements in the goat isolates at the position of 135-138, where Leucine (L), F (Phenylalanine), T (Threonine), and D (Aspartate) sequences changed into S (Serine), L (Leucine), H (Histidine), and L (Leucine), respectively. Furthermore, a replacement in the sequence of amino acid was found in isolates from buffalo at the position of 154, where Serine (S) was transformed into Leucine (L).
CONCLOUSION: The findings our study indicate that the variants of goat and buffalo can be responsible for persistence of Fasciola infection in the endemic areas of Iran. It seems that biological differences could be occurred by considering a variety of F. gigantica-hosts in Iran. Thus, suitable approaches are required for effective treatments and useful control strategies.

VI' 1 protein of foot-and-mouth disease ViruS (FMDV) contains the immonogenic hypervariable region of the virus. The antigenic variation in FMDV is particularly related to the difference between nucleotidc and amino acid sequences of capsid protein. On the basis of this phenomenon. type diagnosis of FMDV can be done by the polymerase chain reaction (PCR). In order to specifically identify the 0, FMDV serotype of Iran the complete coding sequence of its VPI prote in was amplified by RT-PCR, and nuclcotidc and amino acid sequences of the PCR product were dctermincd. The nuclcotide and deduced amino acid sequence exhibited 84% and 88% homology with the VP 1 region of serotype O,K. respectively.

Fourteen isolates of infectious bronchitis virus (IBV) from Iran in 2001 were typed by a type-specific multiplex RT-PCR. The RT-PCR reaction has been designed to detect and differentiate strains of Massachusetts, D274 and 4/91 (793/B) types. Based on the DNA band produced in RT-PCR, twelve isolates were c1assilied in the type Massachusetts and two isolates (13/2001 and 14/2001) in the type 4/91. The identity of isolate 14/2001. as being from the type 4/91, was also confirmed by sequence analysis of its RT -PCR product. The result of this study shows the presence of at least two types of IBV in Iran.