جستجوی مقالات مرتبط با کلیدواژه « Serum » در نشریات گروه « دامپزشکی »

Selenium is a component of some important selenoproteins and enzymes required for main functions in organisms as antioxidant defense, reduction of inflammation, thyroid hormone production, DNA synthesis, fertility, reproduction and killing cancer cells (by reducing the blood supply to tumors). This study was conducted to determine the effects of organic and inorganic selenium (Se) supplementation on serum Se status. 21 healthy mix breed horses 5-8 years of age were randomly divided into three groups, control without Se (CTRL), non-organic bolus (INORG) and organic Se (ORG) and in one of the horse riding clubs in Tehran province. Animals were located in control and treatment groups. Control group received only routine equine diet (were fed a typical alfalfa, grain and straw yields) for 28 days. First treatment group received routine diet plus premix (included 1 mg sodium selenite/day) and a second group was supplemented with 1 mg commercially available –enriched yeast (O-Sel). Blood samples (5ml) were taken by venoject tube from jugular vein on day 0, 14 and 28. Serum selenium levels were measured by atomic absorption method and serum glutathione peroxidase was measured using a biochemical kit. Serum activity of glutathione peroxidase showed a significant difference between the two treatment groups compared to control group (p≤ 0.05). Mean serum Se concentration on d 14 and 28 was greater (P<0.006) for Se-supplemented horses compared with CTRL horses, and tended (P<0.004) to be greater in ORG vs. INORG on d 28. The results of present study showed that use of selenium premix increased blood sera according to results and organic supplementation in horse appeared more efficient and the enzyme glutathione peroxidase could be a good indicator for assessing selenium status in the study groups.

Hemagglutination Inhibition (HI) test is a universal method in veterinary laboratories that has low sensitivity and high specificity in determining the level of antibody. This study aimed to evaluate the repeatability of HI test and to determine the suitable antigen for HI test. Two experiments were conducted in this study. In the first, 60 serum samples were divided into four groups, including negative, low, medium, and high titer serums for Newcastle virus. Also, to evaluate the repeatability of the HI test results, eight pairs of repeating serums were placed from all four serum groups. The sera were sent to 11 different veterinary laboratories, and asked to determine the Newcastle HI titers by standard protocols. In second experiment, 41 sera were used to determine the appropriate antigen using five antigens (Clone Lasota, Lasota, B1, commercial and laboratory antigen) for the HI test. the first experiment showed that most laboratories could identify high titers, but there is a significant difference in determining zero, low, and medium titers. Also, based on results obtained from each of laboratories, it was found that the reproducibility of HI results within a laboratory is low.  In the second experiment, the best antigen was determined in terms of the same answers for use in the HI antigen test of Lasota Clone vaccine. This study is the first study on the quality control of the HI in Iran. The results showed that the quality of test is not satisfactory, and the IVO and research centers should standardize this test.

Peste des petits ruminants (PPR) is an economically important viral disease between sheep and goats that is endemic in Iran. This study was performed to Comparison of Serumic Concentration of Vitamin A and Beta-Carotene in the Serum Positive Against Peste des Petits Ruminant and Healthy Dams in sheep and goats in healthy animals in Khodabandeh area in summer of 2018.During this study, 200 samples were collected from Jugular veins of sheep (150) and goats (50) which were not vaccinated against PPR disease and after serum isolation the samples were analyzed using competitive ELISA kit (ID.vet French). Seroprevalence of PPR in sheepwas 108 cases (72%) and in goats was 36 cases (72%). Seropositive animals were placed in the sick group (144 animals) and Seronegative animals were placed in the healthy group (56 animals). Then Serum levels of Vitamin A and Beta-Carotene measured in all serum samples by hexane method. The mean and standard deviation of vitamin A and Beta-Carotene in serum was 27/6 ± 4/1 mg/dl and 19/5 ± 3/2 mg/dl in diseased group and 58/9 ± 3/2 mg/dl and 19/8 ± 2/1 mg/dl in healthy group, Respectively. Serum level of vitamin A was significantly different between healthy and diseased animals (p<0.05), but Beta-Carotene serum level was not significantly different. The final result is that given the low levels of vitamin A in animals infected with the disease, in areas where the disease is contagious, it can be administered injectable form to provide this vitamin and strengthen the immune system.

Since the the natural delivery mechanism is related to the immunologic process, it is possible that level of different Inflammatory factors involved in immune responses (IL-6 as Pro-Inflammatory Cytokine) may change in the near-parturition time (before and after parturition). The aim of this study is evulation of IL-6 level change in the ewe, one week before until one week after Parturition. For this purpose a total 4 pregnant ewes in the second term of their conception, are selected. Preservation and feeding was same for all of them. Blood sampling were taken from Jugular vein by means of nonobjective and vacuum pipe (5-7cc each 24h, one week before parturition until one week after it). The blood samples for serum separation, were transferred to laboratory and after serum separation, for prevention of otolysis and destruction of immune chains were put in -70ċ. IL-6 was measured useing of specific kit and by Elisa test (Enzyme-linked immunodeficient assay). Results were showed that in the different days before and after parturition the level of IL-6 has slightly changed that is insignificant (P = 0.7625). In total result showed that serum level of IL-6 in different times of parturition doesn’t affect.

Piroplasmosis is a protozoal disease that can cause acute inflammation in the horse. The disease is transmitted by ticks and spread worldwide. Measurement of acute inflammatory phase proteins is important in the diagnosis of inflammation from the disease. This study was conducted to evaluate serum Amyloid A and Haptoglobin levels in horses with pyroplasmosis and compare them with healthy horses. 19 horses with piroplasmosis (12 males and 7 females) were identified on horseback riding around Tabriz and Ardebil in the summer. From the livestock, after taking the history, blood samples were collected from the jugular vein and the serum was isolated. Also, 18 healthy horses (negative blood samples) were also sampled with the same age and nutritional and management status as the healthy group. In each blood sample prepared after serum separation, the serumicheptoglobin and amyloid A values ​​were measured on the basis of immunoassay and specific kits. The mean serumic amyloid A level in horses with piroplasmosis was higher than healthy horses, non significantly (p = 0.164). The mean serum haptoglobin increased in the patient group, significantly (p = 0.014). There was no significant difference between sex and age in serumic levels of these two parameters. The final result is that in piroplasmosis disease in the horse, the serumic levels of amyloid A and heptoglobin increase, and its measurement will be useful in the diagnosis and interpretation of the inflammatory process.

Environmental temperature and photoperiod are among the important factors that may influence the reproductive cycle of various species. The aim of the present survey was to evaluate the different seasonal influences (cold= January and warm= July) and their intermediate seasons (April and October) on serum testosterone concentration in cats of Ahvaz region. For this purpose, one hundred and twenty adult male cats (domestic short hair breed) were examined. Blood samples were taken of thirty cats in each season, and testosterone concentration was measured using ELISA technique in serum samples. The average testosterone concentration of 3.42±0.49 ng/ml was detected in the studied cats. The minimum and maximum of serum testosterone level was also 0.01 and 17.2 ng/ml, in July and January respectively. The minimum of mean testosterone concentration was obtained in the warm season (0.62±0.23 ng/ml), while the maximum mean level was detected in the cold season (9.87±1.28 ng/ml). The mean testosterone concentration in spring and autumn was 2.26±0.5 and 0.94±0.3 ng/ml respectively. Environmental temperature and photoperiod correlated negatively with the testosterone concentration. The correlation coefficient between testosterone concentration with the average of minimum, maximum and total temperature, was calculated as R= -0.572, R= -0.615 and     R= -0.597 respectively. The correlation coefficient indicated an acceptable and high correlation of the negative (inverse) type, between testosterone concentration and photoperiod (R= -0.256). In conclusion, testosterone concentration is under the influence of season, with the mean testosterone concentration significantly lower in summer than the other seasons (p<0.05).

It seems that the evaluation of parameters like serum biochemical changes following intense physical activity can help to provide data which may help in predicting the athletic capacity of horse in racing conditions. In present study, serum concentration of some biochemical factors was determined before and after intense physical activity in nine, 4-10 years old Arab horses. The blood samples were collected at 1 h before (T0) just after )T1(, 3 hours (T2) and 24 hours (T3) after running with maximum speed in a 1250-meters course. The measuring of serum values of total protein, albumine, BUN, creatinine and glucose and also serum activity of creatine phosphokinase and lactate dehydrogenase enzymes were done by routine laboratory methods. The data were analyzed statistically with one-way repeated measures ANOVA and Tukey's test. The results showed that all of the measured parameters, except BUN were significantly increased after running .It seems that heamoconcentration caused by severe sweating and stress induced by racing conditions, are the reason of these changes.

The aim of this study was to improve culture conditions of bovine embryos produced by the Hand-made cloning (HMC) method with the use of serum and myoinositol. For this purpose, after oocyte in vitro maturation followed by elimination of the zona pellucida, bovine oocytes were enucleated by separation of the metaphase spindle protrusion using a very thin pulled Pasteur pipette. After attachment of adult fibroblasts as donor cell into the enucleated oocyte and subsequent fusion and activation, reconstructed embryos were cultured by Well of the Well (WOW) method in six groups, including IVCSOF, IVCSOF-CSS, IVCSOF-ci, IVCSOF-ciCSS, IVCSOFci and IVCSOFci-CSS. In these groups, the embryos were cultured in IVCSOF with or without myoinositol-sodium citrate media until the third and then CSS and/or myoinositol-sodium citrate was added. Finally, development rate was recorded and analyzed statistically. Our result showed that despite the ineffectiveness of myoinositol on embryonic development capabilities, the embryos cultured in the presence of this combination and serum significantly reached earlier to blastocyst stage (p

The present study was conducted to detect the level of serum copper concentration in urban and rural dogs in Ahvaz district, based on the biochemical analysis and atomic absorption spectrophotometery. This survey was accomplished on a group of 250 serum samples of clinically healthy urban and rural dogs with different ages between 2013 and 2014. The urban dogs were selected between referred cases to the Veterinary Hospital of Shahid Chamran University of Ahvaz and the rural dogs were categorized into four areas (north, east, west and south). Classification was made by age, sex, breed, region and diet. The studied dogs were divided into three groups based on age (3 years). The most breed distribution was Mixed (24%), German shepherd (21.6%), Terrier (18.4%), Spitz (11.2%) and Doberman pinscher (9.6%) in the urban dogs. The present results showed that the mean and standard deviation of serum copper concentration was 21.09±0.49 and 15.27±0.29 µmol/L in the urban and rural dogs respectively. Serum copper level was significantly higher in urban dogs than rural dogs (p0.05). This survey showed that the serum copper concentration was significantly higher in urban dogs than rural dogs and in small breeds than large breeds. It seems that, this study is the first research in dogs in Iran.

Serum biochemical parameters are important aspects in the management of endangered species. The values of these parameters can be used for confirming the maturity and for monitoring any changes in the quality of waters and related soils. The aim of present study was to investigate some of blood serum biochemical parameters in immature females and males of Argyrosomus hololepidotus. Blood samples were collected from twenty captured fish from coastal waters of Chabahar (Ramin waterfront). Serum levels of glucose، blood urea nitrogen (BUN)، cholesterol (CHO)، triglyceride، bilirubin، total protein، albumin، phosphorus (P)، sodium (Na)، potassium (K)، calcium (Ca) and alkaline phosphatase (ALP) were determined as Mean±SD in immature males and females. The levels of different biochemical parameters in males and females were respectively as follows; Na (239. 50±17. 06، 257. 80±20. 80)، K (3. 87±0. 98، 1. 94±0. 49)، P (2. 73±0. 39، 2. 79±0. 53)، Ca (2. 89±0. 47، 1. 56±0. 32) (mmol/l)، glucose (71±10. 33، 24. 04±2. 50)، bilirubin 0. 46±0. 11، 0. 24±0. 05)، ALP (68. 30 ±124. 17، 244. 50±68. 98) (Iu/l)، BUN (1. 94±0. 50، 0. 63±0. 29)، triglyceride (94. 70±9. 56، 32. 85±7. 56)، CHO (13. 02±2. 69، 4. 94±3. 29) (mg/dl)، total protein (1. 65±0. 51، 3. 15±0. 86) and albumin (0. 44±0. 16، 0. 12±0. 04) (g/dl). The results showed that the serum levels of Na and total protein in females were significantly higher than males and there were significant differences in all of the serum values (except P) between males and females (P<0. 05). It can be concluded that the values of blood biochemical parameters may be affected by sex of fish.

Minerals are essential materials in animal reproduction. The determination of these elements is valuable in relation to different conditions in Arabian horses. The purpose of this study was to evaluate the effects of pregnancy on certain macro and micro minerals in Arabian horses. Blood samples were collected from one hundred apparently healthy mares from different regions of Ahvaz including 50 pregnant mares (light, medium, heavy) and 50 non-pregnant mares. Macro-minerals (calcium, phosphorus, magnesium, sodium and potassium) and micro-minerals (copper, zinc and iron) were measured, using standard common laboratory methods. Methods of O-cresol phetalein, Molybdate reduction, Xylidil blue were used for measurement of calcium, phosphorus and magnesium respectively. Flame photometry was used for measurement of sodium and potassium. Amounts of copper, zinc and iron were measured by atomic absorption spectroscopy. In order to analyze the results, all the data was statistically compared by SPSS-17.0 software. The study of serum calcium levels between the pregnant and non-pregnant mares showed that the differences were not significant. In this study there was significant differences between serum phosphorus levels between pregnant and non-pregnant mares (p<0.05). The serum phosphorus levels during different stages of pregnancy was significantly different (p<0.05), and the level of serum phosphorus during pregnancy declined in total compared to non-pregnant mares. Serum magnesium did not show significant differences between two groups. Serum sodium levels, compared to the other two groups, did not show significant changes. Changes in serum potassium levels showed significant difference between two groups of pregnant and non-pregnant mares (p<0.05). The level of potassium in pregnant mare’s serum was less than non-pregnant, and the least level of this element was seen in medium pregnancy. Except phosphorus, potassium and zinc, other minerals did not affected different stages of pregnancy. Although the relationship between these changes and pregnancy can be the result of physiological processes, the other factors such as parity, age, nutrition, season, weather conditions and other factors should not be ignored in the evaluation.

The purpose of this study was to investigate the effect of melatonin implant on adrenal function and serum cortisol level in Arabian ewes out of the breeding season. For this purpose، 16 Arabian ewes with 2 to 4 years old (mean weight 35±5 kg) and non-pregnant were divided into two groups. The survey was conducted from late December to early March for 10 weeks. The first group (control) received no melatonin. Implantation of melatonin was used in the second group. Weight of adrenal gland in treatment of melatonin implant in compare with control group is significantly dicrease (p<0. 01). Weight of adrenal gland and thickness of the cortex، capsule، glomerulosa، fasciculate and reticularis in treatment of melatonin implant has been decreased in compare with control group (p<0. 01). In all times of study، there was no significant differences in cortisol levels between melatonin and control treatments (P>0. 05). in control group، the cortisol concentration of blood serum in day 14 was significantly higher than days 42 and 56 (p<0. 01). But in melatonin treatment، there were no significant different in cortisol value among all times of study (P>0. 05). With regard to the results of this study can be said، use of melatonin to induction breeding season in Arabian ewes، not as a stressor and its application in out of the breeding season did not increase the cortisol level.

Staphylococcus aureus is one of the main causes of acne in the all of ages. Many drugs are prescribed to treat it. Utilization of water resources in order to secure access to new drugs is the issues that people are always followed. The present study investigated anti- bacterial effect of the serum of Cyprinus carpio and Hypophthalmychthys molitrix against two strains of Staphylococcus aureus the causative agent of acne. In this study، different concentrations of the serum of C. carpio and H. molithrix1. 16، 1. 32، 1. 4، 1. 2، 1. 8 placed on to nutrient agar and The experimental results of this study were achieved by determining of the minimum inhibitory concentration (MIC) by serial dilution method، the percentage of inhibition of bacterial growth by using absorbance microplate reader device، minimum bacterial concentration (MBC) and the determination of zone growth by the disk diffusion methodantibacterial activity of the serum was evaluated using disc diffusion on two strains of S. aureus. Results of present study indicated that unlike A strain A of S. aureus (No. 48)، strain B bacteria (No. 947) was not sensitive to common carp serum compared with the vancomycin antibiotic. Two strains of S. aureus bacteria were not shown sensitivity to silver carp serum. It can be concluded that C. carpio serum as a drug of natural origin can be used to eliminate strain A of S. aureus.

Negative energy balance in cow occurs in transitional period, high-yielding dairy cows immediately after calving and feed restriction. In response to energy demand cow mobilizes fatty acids from adipose tissue causing an increase in the circulation of NEFA. Increased blood NEFA is associated with low glucose, high free fatty acids and ketone bodies as well as lipid and lipoprotein disturbances in the blood. Does buffalo follows the same as cow, is not yet cleared. The purpose of the present study was to investigate the effects of starvation on some lipid parameters in serum and liver of buffalos in Ahwaz. Five two-year-old male buffalos with average body weight of 200 kg were fattened with a diet containing alpha, barely, wheat, and maze for 8 weeks and then they were fastened for 8 days. During fasting period the animals had free access to barely and water. After fasting period the buffalos were again fattened for 8 weeks. Blood samples were collected from the coccygeal vein during the first and second fattening period with 20 days intervals and every day in the fasting period. Plasma samples were obtained, stored, and analyzed for glucose, BHBA and NEFA using standard kits. The liver of each buffalo was sampled at the beginning and end of feed restriction period and sent to the laboratory for the measurement of the liver TG content. Analyses of variance were carried out to compare mean values in fed and fasted periods. The results of present study revealed that there were no significant differences in plasma NEFA, BHBA and liver TG concentration before and after feed restriction (p>0.05), but there was significant difference in plasma glucose (p<0.05). It appears that in the period of food depravation, buffalos are essentially able to cope with these hard conditions probably through control of lipolysis and gluconeogenesis at the aim of preventing metabolic disorders particularly fatty liver. The results of the present research reveal probably for the first time, this inheritance phenomenon with the emphasis of further research.