Detection of somatic antigens in Mycobacterium avium subspecies paratuberculosis

Bakground: Johne's disease or Paratuberculosis as a chronic granulomatosis enteritis in ruminants will be caused by Mycobacerium avium subsp. Paratuberculosis. Detecting whole bacterial cell wall antigens would be helpful in potential applications for diagnosis, vaccine production, and elucidation of pathogenesis. To determine secreted somatic cell antigens of Mycobacterium avium subspecies Paratuberculosis. Standard strain (III-V) of Mycobacterium avium subspecies Paratuberculosis DNA was extracted from the cultured and gene analysis was done using PCR to confirm bacterial purity. On the other hand, protein concentrations in both media and cell entracts were determined. Furthermore, all proteins pattern were shown by SDS -PAGE. Electrophoretic findings showed some somatic antigens in the range of 19 - 100 KDa. These purified somatic antigens can be used for further study and potential application in vaccine production.