Investigation on preservative effects of Aspirin on Human Hemoglobin glycation in diabetic condition

Diabetes is a common disease which is characterized by hyperglycemia and the increase of protein glycation. The aim of this study was to investigate the effect of aspirin-induced damage in human hemoglobin in diabetic glycation. In this study، hemoglobin extracted from the blood of healthy individuals was incubated in the presence and absence of glucose and aspirin for 5 weeks. The rate of haem glycotation was determined in different conditions by studding products of Heam degradation، sort-band shifting and febrile state. Data were analyzed using One-Way analysis of Variance and Tukey’s test. In the presence of aspirin، the amount of glycation reduced 50%. Furthermore، studies using band-shift sorting and febrile status indicated significant reduce in the amount of protein glycation in the presence of Aspirin. Aspirin reduces extent of glycation when hemoglobin is incubated in the presence of glucose. Likely، aspirin exerts its effect by acetylating amine groups in proteins.