ffects of Aerobic Training on Tissue Nesfatin-1/Nucleobindin-2 mRNA, Plasma Nesfatin-1 and High-density Lipoprotein Concentration in Female Rats

Nesfatin-1 is a protein derived from a precursor molecule of the nucleobindin-2 gene. Nesfatin-1 has been suggested to act as a novel inhibitor and a potential regulator of food intake and body weight. It has been reported that changes in proteins and peptides that are released from hypothalamus and adipose tissue are related to cardiovascular diseases (CAD). Therefore, the aim of this study was investigate the effects of eight weeks of aerobic training on nesfatin-1/nucleobindin-2 expression, plasma concentration, and plasma high-density lipoprotein (HDL-C) in female rats (Liver, small intestine and kidney tissues). In this study ten Wistar rats (6-8 weeks old and 125-135 g weight) were used. Animals were randomly assigned into control and training groups. Training group was given exercise on a motor-driven treadmill at 25 m/min (0% grade), 60 min/day, 5 days/week, for eight weeks. 72 hours after the last training session and after four hours of fasting, rats were sacrificed. Liver, small intestine and kidney were excised, total RNA was extracted, and after PCR procedure, quantitative real-time RT-PCR was performed. Plasma was also collected for plasma variable measurement. Data are expressed as means ± SEM and the significance level was set at P