Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system
Author(s):
Abstract:
Objective
This study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis Materials And Methods
Isolated testicular cells were cultured in DMEM/F12. Characteristics of the isolated cells and obtained ES-like cell were immune-cytochemically confirmed by examining the presence of PLZF, vimentin, Oct4 and Nanog protein. Expression of the pluripotency and germ-cell specific genes was analyzed by qPCR in derived ES-like colony and SSCs respectively. Results
The experiment results indicated that our method of obtaining pluripotent ES-like cells from spermatogonial cells (SCs) is simpler than the described methods. ES-like cells were immunopositive for pluripotency markers. ES-like cell qPCR results indicated significant increase in pluripotency genes expression and significant decrease in germ cell-specific genes expression. Conclusion
The results indicated that ES-like cell with pluripotency characteristic were generated from freshly isolated spermatogonial cells. The pluripotent stem cells provide a cellular reservoir usable for regenerative medicine instead of embryonic stem cells.Keywords:
Language:
English
Published:
International Journal of Reproductive BioMedicine, Volume:12 Issue: 1, Jan 2014
Pages:
37 to 46
https://magiran.com/p1237058
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