Designing a phage-derived gene nanocarrier and examination of its capacity for delivering and expression of transgene in human cell line

Bacteriophage lambda comprises critical advantages that have made it as an ideal gene delivery vehicle into the eukaryotic cells. These advantages reside in both the structure and biology of the lambda phage، for example، recent investigations have revealed common ancestry for the tailed phages and eukaryotic DNA viruses. Considering these features as well as the double-stranded nature of its genome، bacteriophage lambda might comprise a great advantage for phage-mediated gene delivery into eukaryotic cells. A phage lambda-derived gene nanocarrier bearing reporter gene GFP (λ-GFP) was constructed and utilized to transfect AGS cell line. λ-GFP particle-mediated gene delivery and expression as well as its internalization by AGS cell line was investigated.