Histopathological and biochemical study of Allium hirtifolium and Sodium Phenytoin on healing of cutaneous lesions in Common carp

Cutaneous lesions of fish from viral، bacterial، parasitic and chemical and mechanical factors are common. The rapid progress of infection and the mortality of fish need more attention. Allium hirtiofolium is a native plant in Iran which contains saponins، it has a role in inhibiting and killing cancer cells with anti-allergenic، anti-bacterial، anti-inflammatory and anti-virus effects. In this Study 120 Common Carp (10 ± 60 g) with 3% NaCl solution was sterilized for 15 minutes. At the beginning of the test a long cut 1. 5 cm and in depth of 2 mm in the rear (left side) of all of the fish was created. Afterward they were divided into 3 groups (n=40 in each)، Group 1 (control)، group2 (treated with5 mg / lit Sodium phenytoin) and group3 (treated with 5 mg / lit Allium hirtifolium). Days 0، 3، 6، 14 and 20 tests، seven fish from each group were randomly selected after that blood and tissue samples were taken from the site of the lesion. The results of the biochemical changes showed that difference in cholesterol and liver enzymes aspartate amino transferase (AST) and alanine amino transferase (ALT) in group3 compared to the control group was significant (P <0. 05)، in triglyceride was found a significant difference and it was higher in groups 3 and 1 (p <0. 05)، but No significant difference in blood glucose between groups were observed in any of the test days Between group1 and 3. Histopathology results obtained from 3 groups showed that in the group treated with phenytoin sodium healing was in less time than in group3. According to the results، further studies are recommended.