The effect of Dorema ammoniacum pretreatment on the progression of seizures induced by chemical kindling in rats

The purpose of this study is to investigate the effect of Dorema ammoniacum on PTZ induced seizures in male rats. In chemical kindling، animals received pentylenetetrazol، 30mg/kg/48h، intraperitoneally and five different stages of seizure were appeared gradually and seizure parameters including maximum seizure stage (SS)، stage 4 latency (S4L)، and seizure duration (SD) were measured during 20 min after PTZ injection. Subsequently، the effects of Dorema ammoniacum (50 and 100mg/kg)، injected 30 min before PTZ، on these parameters were evaluated and compared with antiepileptic drug، phenobarbital (30 mg/kg، IP). Results showed that Dorema ammoniacum decreased SS and SD and increased S4L significantly (P<0. 001، P<0. 01، respectively). This antiepileptic effect was as much as 50%-60% of phenobarbital effects. On the basis of obtained results it may be concluded that Dorema ammoniacum has anticonvulsant effect on PTZ induced kindling model of epilepsy. Considering that Dorema ammoniacum increased the latency of seizure stages occurrence and reduced duration of different seizure stages (SD) and seizure stage (SS)، it may prevent spreading of epileptiform activity in the nervous system and contribute to turn off excessive neuronal firing.