Antimicrobial Effect of Portulaca oleracea Extract on Infectious Microorganisms- In-vitro Study

Today with raised use of antibiotics and prevalence of resistant strains، there is need for antimicrobial drugs that have fewer side effects than antibiotics. The aim of this study is to investigate the antimicrobial effect of Portulaca oleracea on Escherichia coli PTCC 1330،Staphylococcu aureus PTCC 1337، Staphylococcus epidermidis PTCC 1435، Streptococcus pyogenes PTCC 1447 and Pseudomonas aeruginosa PTCC 1310. In this in vitro study Portulaca oleracea was used to evaluate its antimicrobial effects. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the microplate method. Data was analyzed by SPSS18 software and one-way ANOVA test analysis and Tukey test. p <0. 05 was considered significant. In this study، the most efficacy of ethanol extract of Portulaca oleracea was at concentration of 100 mg/ml against Streptococcus pyogenes and Staphylococcus epidermidis. MIC of ethanolic extract for Streptococcus pyogenes، Staphylococcus epidermidis، Staphylococcu aureus، Escherichia coli and Pseudomonas aeruginosa was 6. 25،6. 25، 12. 5،25 and 50 mg/ ml and their MBC of the was 6. 25، 12. 5، 12. 5، 50 and 50 mg/ml، respectively. MIC of aqueous extract for Streptococcus pyogenes، Staphylococcus epidermidis، Staphylococcu aureus، Escherichia coli andPseudomonas aeruginosa was 12. 5، 12. 5، 25، 50 and 100 mg/ ml and their MBC of the was 25، 25، 50، 100 and 200 mg/ml، respectively. The results of this study showed that Portulaca oleracea can be used instead of synthetic antimicrobial that their resistance against microbial increased daily.